Team:Heidelberg/Demonstrate

iGEM TEAM HEIDELBERG 2017

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Demonstrate

Our project works

In vivo Evolution

Demonstration of our PREDCEL Toolbox:

Cytochrome engineering

We engineered the product specificity of cytochrome P450 1A2 using PREDCEL.

Protein Interaction

We used PREDCEL to evolve a split T7 polymerase towards improved auto-reassembly.

Optogenetics

We used light to control selection stringency during PREDCEL

In silico Evolution

Demonstration of our AiGEM Software:

Evolution of \(\beta\)-Lactamases

We observed a correlation of the measured \(\beta\)-lactamase activities with the protein label scores from AiGEM

Reprogramming of \(\beta\)-Glucoronidase

We used AiGEM to inform the design of a \(\beta\)-glucoronidase mutant with near-wildtype \(\beta\)-galactosidase activity.

Organosilicons

Demonstration of Carbon-Silicon-Bond formation:

Organosilicons

We used an engineered cytochrome to catalyze carbon-silicon bond formation in whole-cell extracts.