Team:KAIT JAPAN/result
RESULT
Construction of Cure allergic Rhinitis System
- Plasmid 1
- IL-10Receptor, STAT1 and JAK are cloned to produce plasmid 1.
- Figure below shows colonies observation images obtained by binding all genes, placing them in pSB1C3 and transforming them into DH5α.
- Picture of agar gel electrophoresis result of plasmid extraction from the obtained colony is shown in figure below.
- From figure above, we can confirm that the plasmid obtained is consistent with the molecular weight targeted.
- Plasmid 2
- Total: about 7443bp
- In order to create plasmid 2 (4 patterns), GFAPpromoter, HlyD, HlyB, TolC, and HlyA + IL-12 or GFP were cloned.
- This four plasmids has the same molecular weight.
- The four figure below shows colony observation image obtained by binding all genes and inserts them in pSB1C3 and transforms them into DH5α.
- Agarose gel electrophoresis result of plasmid extraction from the obtained colonies is shown in figure below.
- From figure above, we can confirm that all the plasmids obtained are plasmid 2 since the molecular weight is consistent with the molecular weight targeted.
Contact: kaitjapan@gmail.com Twitter:@KAIT_JAPAN
