Team:Stockholm/Protocols

A mucus degrading lung probiotic

Protocols

Protocols that was used during the project.

• 3A Assembly
• Amicon Ultra Filtration
• DNA Kit Plate
• Endo-β-galactosidase assay
• Gel Electrophoresis
• Gel Purification
• Glycerol Stocks
• IMAC
• Ligation
• Media and Agar
• Osmotic Pressure Testing
• PCR Purification
• Phusion High Fidelity PCR
• Q5 High Fidelity 2X PCR
• Q5 High Fidelity PCR
• Taq DNA Polymerase PCR
• Plasmid Purification
• Protein Expression
• Restriction Digestion
• Sialidase Assay
• Sonication
• Transformation
• SDS-PAGE

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