Nowadays intracellular products take up a great share o the market, and with the increase of people’s need, they will create a growing market value. But the way to extra the target products from inside of the cells has always been a problem. Chemical disruption ways will mix in new impurities which need to be separated later, and physical ways may bring high temperature leading to unstable products so they need chillers. Considering of this problem, we came up with an idea that if cells could self-lyse under the control of some convenient conditions provided by environment, for example, the light, because light serves as an excellent trigger to achieve precise control on synthetic systems as it can regulate in wavelength, timing, intensity and location. Based on this thought, the project showed in the video just came out. In particular, we focused on Escherichia coli in the project because it’s common used and easy to operate. The project may fit other bacteria in further study.

References and sources we used in our research.

Wu H, Wang Y, Wang Y, Cao X, Wu Y, Meng Z, Su Q, Wang Z, Yang S, Xu W, Liu S, Cheng P, Wu J, Khan MR, He L, Ma G., Quantitatively relating gene expression to light intensity via the serial connection of blue light sensor and CRISPRi. ACS Synth Biol. 2014, 3(12): 979-982
Gardner L, Deiters A. Light-controlled synthetic gene circuits. Curr Opin Chem Biol. 2012, 16(3-4):292-299
Camsund D, Lindblad P, Jaramillo A. Genetically engineered light sensors for control of bacterial gene expression. Biotechnol J. 2011, 6(7):826-836
Ohlendorf R, Vidavski RR, Eldar A, Moffat K, Möglich A., From dusk till dawn: one-plasmid systems for light-regulated gene expression. J Mol Biol. 2012, 416(4): 534-542
Sun Q, Kuty GF, Arockiasamy A, Xu M, Young R, Sacchettini JC., Regulation of a muralytic enzyme by dynamic membrane topology. Nat Struct Mol Biol. 2009 Nov;16(11):1192-1194

Team:ZJUT-China/Description

ZJUT-China

Description

Conception

References and sources we used in our research.