Team:ZJUT-China/Improve
ZJUT-China
Improve
Gold Medal Criterion #2
We improved the characterization of one existing BioBrick Part this year.
The existing part is :BBa_K1065303
The improved part is :BBa_K2277555
To improve sensitivity of the light sensing system, we made modifications in the following aspects:
- Replaced the lacIq promoter with the IGEM standard element promoter J23100
- Added IGEM standard element terminator B001 at the downstream of egfp
- Replaced mrfp with egfp
- Replaced the pBR322 origin with pUC origin
- Replaced the original promoter PFixK2 with P’FixK2 documented in the reference (J Mol Biol, 2012, 416: 534-42)
Sequences
Original promoter PFixK2
GCCGG AGCCG ATTAT CCGCA CCCGT CCTTG GTCTT GGACA CACTC GCTCG
CGATC CGAAG CCGCC TTTCA AAGAT ACTCC GCAGT GAAAC GCATC TTTTA
AGCGC GACTT GTCGC CTTCG CGCAG CAGAA CACTC GTATG GCACT CAATC
CATGA TCGCT TCGTT CGCTC CGACG CGCGT TGCGG GGCCC CCCTC GACCG
GATGA CAACA CATTG CGTCA CCTCA ACTGC TGCGC GCGCA CTGCG TCACA
Replaced promoter PFixK2
ACGCC CGTGA TCCTG ATCAC CGGCT ATCCG GACGA AAACA TCTCG ACCCG
GGCCG CCGAG GCCGG CGTAA AAGAC GTGGT TTTGA AGCCG CTTCT CGACG
AAAAC CTGCT CAAGC GTATC CGCCG CGCCA TCCAG GACCG GCCTC GGGCA
TGACC TACGG GGTTC TACGT AAGGC ACCCC CCTTA AGATA TCGCT CGAAA
TTTTC GAACC TCCCG ATACC GCGTA CCAAT GCGTC ATCAC AACGG AG
