Difference between revisions of "Team:William and Mary/Results"

Line 11: Line 11:
  
 
<link rel="stylesheet" href="https://cdnjs.cloudflare.com/ajax/libs/font-awesome/4.7.0/css/font-awesome.min.css">
 
<link rel="stylesheet" href="https://cdnjs.cloudflare.com/ajax/libs/font-awesome/4.7.0/css/font-awesome.min.css">
 +
<link href="https://fonts.googleapis.com/css?family=Modak" rel="stylesheet">
 +
 +
<style>
 +
      body {
 +
        font-family: 'Modak', cursive;
 +
        font-size: 48px;
 +
      }
 +
 +
</style>
 +
 +
</head>
 
          
 
          
 +
<body>
 +
 +
<br>
 +
</br>
  
−
<div>
 
 
<center>
 
<center>
−
<img src="https://static.igem.org/mediawiki/2017/d/d6/T--William_and_Mary--resultstitle8.jpeg" height = "12%" width = "100%"/>
+
  <div>This part is designed to easily facilitate appending the protein degradation tag (pdt)#3 tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.</div>
−
</center>
+
 
</div>
 
</div>
 +
</center>
 +
  
  
 +
</body>
  
  

Revision as of 19:14, 15 October 2017




This part is designed to easily facilitate appending the protein degradation tag (pdt)#3 tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.