Difference between revisions of "Team:AshesiGhana"

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    background-image: url(https://static.igem.org/mediawiki/2017/2/28/T--AshesiGhana--backgroundPattern.jpg);
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<h1> Welcome to iGEM 2017! </h1>
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    <body>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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</div>  
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<div class="clear"></div>
 
  
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<h5>Before you start: </h5>
 
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<p> Please read the following pages:</p>
 
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<ul>
 
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<li>  <a href="https://2017.igem.org/Competition">Competition Hub</a> </li>
 
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<li> <a href="https://2017.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
 
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<li> <a href="https://2017.igem.org/Resources/Template_Documentation">Template documentation</a></li>
 
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</ul>
 
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</div>
 
  
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<div class="highlight">
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<body>
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<h5> Styling your wiki </h5>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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<div class="column full_size" >
 
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<h5> Wiki template information </h5>
 
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2017.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
 
  
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                    <!-- Wrapper for slides -->
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                                    <h2>Meet the team solving a national crisis</h2>
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                                    <p>
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                                        This is a team of bright scientists and engineers who have gathered the courage to engineer a biological solution for one of their nation's biggest problems. 
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                                    </p>
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                                </div>
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                        <div class="item two">
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<h5> Editing your wiki </h5>
 
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
 
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<p> <a href="https://2017.igem.org/wiki/index.php?title=Team:Example&action=edit"> </a>Use WikiTools - Edit in the black menu bar to edit this page</p>
 
  
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                            <div class="text-center">
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                                <!--<div class="shell-custom bck">
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                                    <h1 class="fh5co-intro-lead animate-1 to-animate"> Team<br>AshesiGhana</h1>
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                                    <h2 class="fh5co-intro-sub animate-2 to-animate">Introducing the Greatest Gold Miners in the World....Microbes</h2>
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                                </div>-->
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                            </div>
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                        </div>
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<div class="carousel-caption">
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    <div class="shell-custom bck">
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                                <h2>Obuasi Gold Fields </h2>
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                                <p>Mining fields like these have made Ghana  one of the world's leading producers of Gold. However the scent of Gold attracts people who do not always have much care for the environment. Learn More About <a href = "https://en.wikipedia.org/wiki/Galamsey">Galamsey</a></p>
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                            </div>
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                                    <h2>Pioneer Team from Ghana</h2>
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                                    <p>From a well meshed background of Engineering and Computer Science, unfazed by the challenges of Synthetic Biology and fuelled by a drive to Solve Problems that impact our scoiety. </p>
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<h5>Tips</h5>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<ul>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Be clear about what you are doing and how you plan to do this.</li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2017.igem.org/Calendar">iGEM 2017 calendar</a> </li>
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<li>Have lots of fun! </li>
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</ul>
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            </div>
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        </div>
  
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<h5>Inspiration</h5>
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</header>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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    <!--Banner ends here-->
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<ul>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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</ul>
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<h5> Uploading pictures and files </h5>
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<p> You can upload your pictures and files to the iGEM 2017 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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When you upload, set the "Destination Filename" to <br><code>T--YourOfficialTeamName--NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)<br><br>
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UPLOAD FILES
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                                <h1 class="fh5co-intro-lead animate-1 to-animate">Team AshesiGhana</h1>
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                                <h2 class="fh5co-intro-sub animate-2 to-animate">Introducing the Greatest Gold Miners in the World....Microbes</h2>
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                        <h2 class="fh5co-section-heading to-animate">Project Description<span class="fh5co-border"></span></h2>
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                                <p>For the 2017 IGEM, Team AshesiGhana is working on a bio-mining project. The objective of our project is to develop an organism capable of liberating gold from the refractory ore. We aim to provide not only an alternative, non-toxic approach for small scale mining, but also deliver an easy and fast bio-detection and quantification method of ore in the mining industry of our country. The availability of such a biosensor will allow routine monitoring of the ore before a mining endeavor is undertaken thus preventing the destruction of the environment. The project will engineer the typical environmental organism Acidithiobacillus ferroxidans with a FRET probe.</p>
  
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                                <p>This new organism will primarily be capable of sensing and quantifying the amount of gold in the ore. This will be accomplished by using a two-part probe, a donor part which is made up of gold binding protein (golB) attached to a green fluorescent protein (nowGFP). The second part of the acceptor is also made up of a binding protein and a red fluorescent protein (mRuby2). In the presence of a high amount of free gold, the two parts would be in close proximity and energy transfer can take place and the red protein would be excited giving off a fluorescent signal. Using calibration experiments, we can relate the amount of fluorescence to the amount of gold present,liberated by the organism from the ore.</p>
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                                <p>As Acidithiobacillus ferroxidans is a difficult organism to grow in large quantities, we will engineer a strain of E coli to produce two of the main oxidizing enzymes for iron and sulphite which will liberate the gold from the ore. The organism will also be engineered with protective enzymes against the low pH, which is one of the bi-products of the gold liberation reaction, and metal toxicity. The same FRET biosensor part can be added to the E coli strain for the quantification of gold. This new organism can easily be grown in large batches and so can be used to extract gold from refractory ore without the need of any toxic treatment thus providing a safe alternative for small scale mining. </p>
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{{Template:AshesiGhana/Footer}}

Latest revision as of 19:50, 27 October 2017

Ashesi University
College

Project Description

For the 2017 IGEM, Team AshesiGhana is working on a bio-mining project. The objective of our project is to develop an organism capable of liberating gold from the refractory ore. We aim to provide not only an alternative, non-toxic approach for small scale mining, but also deliver an easy and fast bio-detection and quantification method of ore in the mining industry of our country. The availability of such a biosensor will allow routine monitoring of the ore before a mining endeavor is undertaken thus preventing the destruction of the environment. The project will engineer the typical environmental organism Acidithiobacillus ferroxidans with a FRET probe.

This new organism will primarily be capable of sensing and quantifying the amount of gold in the ore. This will be accomplished by using a two-part probe, a donor part which is made up of gold binding protein (golB) attached to a green fluorescent protein (nowGFP). The second part of the acceptor is also made up of a binding protein and a red fluorescent protein (mRuby2). In the presence of a high amount of free gold, the two parts would be in close proximity and energy transfer can take place and the red protein would be excited giving off a fluorescent signal. Using calibration experiments, we can relate the amount of fluorescence to the amount of gold present,liberated by the organism from the ore.

As Acidithiobacillus ferroxidans is a difficult organism to grow in large quantities, we will engineer a strain of E coli to produce two of the main oxidizing enzymes for iron and sulphite which will liberate the gold from the ore. The organism will also be engineered with protective enzymes against the low pH, which is one of the bi-products of the gold liberation reaction, and metal toxicity. The same FRET biosensor part can be added to the E coli strain for the quantification of gold. This new organism can easily be grown in large batches and so can be used to extract gold from refractory ore without the need of any toxic treatment thus providing a safe alternative for small scale mining.