We submitted four parts to the Registry (see list below). Unfortunately, some parts that we’ve worked with are under patent, like SCI-57 and PenShuf, and though we had no legal problems working with it in the lab, we could not submit or make any profits and have credit for these parts.
We also had problem cloning some parts and we were not able to submit them: the usp45 promoter and the signal peptide from Lactococcus lactis, and the cell-penetrating peptide penetratin. However, we have submitted interesting and novel parts to the Registry: BBa_K2270010: a super-folding GFP codon-optimized that exhibits strong fluorescence in L. lactis. We’ve characterized this part, providing future teams an useful reporter to be used in constructions with L. lactis as chassis; BBa_K2270005: the regulatory promoter from the gal operon of L. lactis repressed by the presence of glucose and can be useful for many future constructions; BBa_K2270006: a synthetic small RNA that regulates the gene expression at posttranscriptional level and can be used in different constructions, under the control of different promoters and controlling the gene expression of any gen designed with the 5’ sequence complementary to the sRNA sequence. Thus, we provided a new alternative to regulate the gene expression in bacteria.

Our parts help to fill the lack of Lactococcus lactis parts that we found in the Registry and can also be useful to stimulates other teams to work with L. lactis as chassis, that has been recently used for many applications as a platform for protein expression and therapeutics.

Furthermore, we have improved the srfA promoter (long variant) from Bacillus subtilis (BBa_K305008) that we’ve used in one of our constructions. We have characterized this promoter and showed that it’s stronger that the veg promoter, considerate a strong B. subtilis promoter. Thus, we’ve contributed to improve the Bacillus subtilis collection in the Registry and we offer future teams a new option when choosing a constitutive strong promoter for their devices.

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