Difference between revisions of "Team:BostonU HW/Demonstrate"

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<h3>★  ALERT! </h3>
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.text_section{
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<div class="column full_size">
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<h1>Demonstrate</h1>
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<h3>Gold Medal Criterion #4</h3>
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<p>
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Teams that can show their system working under real world conditions are usually good at impressing the judges in iGEM. To achieve gold medal criterion #4, convince the judges that your project works. There are many ways in which your project working could be demonstrated, so there is more than one way to meet this requirement. This gold medal criterion was introduced in 2016, so check our what 2016 teams did to achieve a their gold medals!
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</p>
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<p>
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Please see the <a href="https://2017.igem.org/Judging/Medals">2017 Medals Page</a> for more information.
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</head>
  
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<body>
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<!-- *************THIS IS WHERE PAGE STARTS************* -->
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<div class="landing-page">
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<div class="wrapper" id="page_background">
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<div class="header" id="Header_Pic">
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<img src="https://static.igem.org/mediawiki/2017/0/02/MARSbackground.png" id="BACKGROUND">
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<img src="https://static.igem.org/mediawiki/2017/2/22/MARSLogo2.png" id="MARS">
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<img src="https://static.igem.org/mediawiki/2017/e/e3/MARS_Repository.png" id="TITLE">
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</div>
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<div class="main main-raised" id = "main_text" style="margin-bottom:5%;">
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<div class="container">
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<h1 class="text-center"> Explore the MARS Repository</h1>
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<div class="text_section col-md-12">
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<div class="text">
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When the team began designing the MARS Repository, we wanted to base it on the principles behind most complex synthetic biology protocols.
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<br>
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<br>
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We began by generalizing synthetic biology protocols into three key stages:
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<ol>
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<li>Isolation: Acquiring the desired genetic material</li>
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<li>Modification: Manipulated or processing genetic material</li>
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<li>Quantification: Measuring the success of the previous two stages</li>
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</ol>
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These stages “fit” together to form the entirety of the protocols that are carried out everyday in synthetic biology labs.
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<br>
 +
<br>
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In the existing fields of microfluidics, each of these stages is taken care of on individual chips. However, when it comes time to replicate a full synbio procedure using these chips - it becomes difficult to “fit” them together realistically. This is due to the fact that most chips:
 +
<ol>
 +
<li>Fabrication using different and complicated techniques such as soft lithography</li>
 +
<li>Operated using varying hardware</li>
 +
<li>Lack thorough documentation required to run them</li>
 +
<li>Do not have easily accessible design files </li>
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</ol>
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Furthermore, the majority of these chips are highly specialised to specific experimental protocols. As a result, they are not relevant in the context of day-to-day procedures in most synthetic biology labs.
 +
<br>
 +
<br>
 +
After consultation with researchers at BU’s Biological Design Center, we were able to identify nine essential synthetic biology protocols and generalize them into these three categories. After confirming that this chip selection was relevant with an iGEM team poll, we moved on to designing and iterating on chips. The resulting design files, fabrication instructions and usage protocols are housed here - easily accessible to synbiologists and structured in a standardised workflow.
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</div>
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</div>
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<div class="col-md-4">
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<div class="text_section row">
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<h3 class="text-center"><u>Isolation</u></h3>
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<div class="text">
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Lorem ipsum dolor sit amet, consectetur adipiscing elit. Integer volutpat, mi feugiat blandit feugiat, ante nisl dictum lacus, in egestas ligula nulla eu ex. Quisque vel justo congue, volutpat neque at, placerat nulla. Nam malesuada tellus sed justo pretium, eu vulputate mauris elementum. Donec elementum gravida ipsum id rhoncus.
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</div>
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</div>
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</div>
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<div class="col-md-4">
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<div class="text_section row">
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<h3 class="text-center"><u>Modification</u></h3>
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<div class="text">
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Lorem ipsum dolor sit amet, consectetur adipiscing elit. Integer volutpat, mi feugiat blandit feugiat, ante nisl dictum lacus, in egestas ligula nulla eu ex. Quisque vel justo congue, volutpat neque at, placerat nulla. Nam malesuada tellus sed justo pretium, eu vulputate mauris elementum. Donec elementum gravida ipsum id rhoncus.
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</div>
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</div>
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</div>
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<div class="col-md-4">
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<div class="text_section row">
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<h3 class="text-center"><u>Quantification</u></h3>
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<div class="text">
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Lorem ipsum dolor sit amet, consectetur adipiscing elit. Integer volutpat, mi feugiat blandit feugiat, ante nisl dictum lacus, in egestas ligula nulla eu ex. Quisque vel justo congue, volutpat neque at, placerat nulla. Nam malesuada tellus sed justo pretium, eu vulputate mauris elementum. Donec elementum gravida ipsum id rhoncus.
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</div>
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</div>
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</div>
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</div>
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<div class="container">
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<div class="col-md-12">
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<div class="row">
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<h3><u>Isolation</u></h3>
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<div class="column col-md-4">
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<a href="https://2017.igem.org/Team:BostonU_HW/Lysis"><img src="https://static.igem.org/mediawiki/2017/0/09/MARS_Matrix_Lysis.png" alt="Picture"></a>
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<h4 class="text-center">Cellular Lysis</h4>
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</div>
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<div class="column col-md-4">
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<a href="https://2017.igem.org/Team:BostonU_HW/Digestion"><img src="https://static.igem.org/mediawiki/2017/c/cd/MARS_Matrix_Digest.png" alt="Picture"></a>
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<h4 class="text-center">DNA Digestion</h4>
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</div>
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<div class="column col-md-4">
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<a href="https://2017.igem.org/Team:BostonU_HW/Ligation"><img src="https://static.igem.org/mediawiki/2017/5/5e/MARS_Matrix_Ligation.png" alt="Picture"></a>
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<h4 class="text-center">Ligation</h4>
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</div>
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</div>
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<div class="row">
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<h3><u>Modification</u></h3>
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<div class="column col-md-4">
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<a href="https://2017.igem.org/Team:BostonU_HW/Transformation"><img src="https://static.igem.org/mediawiki/2017/5/5b/MARS_Matrix_Trans.png" alt="Picture"></a>
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<h4 class="text-center">Transformation</h4>
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</div>
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<div class="column col-md-4">
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<a href="https://2017.igem.org/Team:BostonU_HW/PCR"><img src="https://static.igem.org/mediawiki/2017/6/63/MARS_Matrix_PCR.png" alt="Picture"></a>
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<h4 class="text-center">PCR</h4>
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</div>
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<div class="column col-md-4">
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<a href="https://2017.igem.org/Team:BostonU_HW/Culturing "><img src="https://static.igem.org/mediawiki/2017/f/fb/MARS_Matrix_CC.png" alt="Picture"></a>
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<h4 class="text-center">Cell Culturing</h4>
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</div>
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</div>
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<div class="row">
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<h3><u>Quantification</u></h3>
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<div class="column col-md-4" style="margin-bottom:3%;">
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<a href="https://2017.igem.org/Team:BostonU_HW/Antibiotic"><img src="https://static.igem.org/mediawiki/2017/c/cc/MARS_Matrix_AR.png" alt="Picture"></a>
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<h4 class="text-center">Antibiotic Resistance</h4>
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</div>
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<div class="column col-md-4">
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<a href="https://2017.igem.org/Team:BostonU_HW/Fluorescence"><img src="https://static.igem.org/mediawiki/2017/d/d6/MARS_Matrix_Flo.png" alt="Picture"></a>
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<h4 class="text-center">Fluorescence</h4>
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</div>
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<div class="column col-md-4">
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<a href="https://2017.igem.org/Team:BostonU_HW/Isolation"><img src="https://static.igem.org/mediawiki/2017/8/8e/MARS_Matrix_CS.png" alt="Picture"></a>
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<h4 class="text-center">Cell Sorting</h4>
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</div>
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</div>
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</div>
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</div>
 
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<h4> What should we do for our demonstration?</h4>
 
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<h5> Standard teams </h5>
 
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<p>
 
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If you have built a proof of concept system, you can demonstrate it working under real world conditions. If you have built a biological device that is intended to be a sensor, can you show it detecting whatever it is intended to sense. If it is intended to work in the field, you can show how this might work using a simulated version in the lab, or a simulation of your device in the field.<strong> Please note biological materials must not be taken out of the lab</strong>.
 
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<h5> Special track teams </h5>
 
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<p>
 
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Special track teams can achieve this medal criterion by bringing their work to the Jamboree and showcasing it in the track event. Art & Design, Measurement, Hardware and Software tracks will all have showcase events at the Giant Jamboree.<strong> Please note biological materials must not be taken out of the lab</strong>.
 
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Revision as of 21:23, 28 October 2017

BostonU_HW

Repository

Explore the MARS Repository

When the team began designing the MARS Repository, we wanted to base it on the principles behind most complex synthetic biology protocols.

We began by generalizing synthetic biology protocols into three key stages:
  1. Isolation: Acquiring the desired genetic material
  2. Modification: Manipulated or processing genetic material
  3. Quantification: Measuring the success of the previous two stages
These stages “fit” together to form the entirety of the protocols that are carried out everyday in synthetic biology labs.

In the existing fields of microfluidics, each of these stages is taken care of on individual chips. However, when it comes time to replicate a full synbio procedure using these chips - it becomes difficult to “fit” them together realistically. This is due to the fact that most chips:
  1. Fabrication using different and complicated techniques such as soft lithography
  2. Operated using varying hardware
  3. Lack thorough documentation required to run them
  4. Do not have easily accessible design files
Furthermore, the majority of these chips are highly specialised to specific experimental protocols. As a result, they are not relevant in the context of day-to-day procedures in most synthetic biology labs.

After consultation with researchers at BU’s Biological Design Center, we were able to identify nine essential synthetic biology protocols and generalize them into these three categories. After confirming that this chip selection was relevant with an iGEM team poll, we moved on to designing and iterating on chips. The resulting design files, fabrication instructions and usage protocols are housed here - easily accessible to synbiologists and structured in a standardised workflow.

Isolation

Lorem ipsum dolor sit amet, consectetur adipiscing elit. Integer volutpat, mi feugiat blandit feugiat, ante nisl dictum lacus, in egestas ligula nulla eu ex. Quisque vel justo congue, volutpat neque at, placerat nulla. Nam malesuada tellus sed justo pretium, eu vulputate mauris elementum. Donec elementum gravida ipsum id rhoncus.

Modification

Lorem ipsum dolor sit amet, consectetur adipiscing elit. Integer volutpat, mi feugiat blandit feugiat, ante nisl dictum lacus, in egestas ligula nulla eu ex. Quisque vel justo congue, volutpat neque at, placerat nulla. Nam malesuada tellus sed justo pretium, eu vulputate mauris elementum. Donec elementum gravida ipsum id rhoncus.

Quantification

Lorem ipsum dolor sit amet, consectetur adipiscing elit. Integer volutpat, mi feugiat blandit feugiat, ante nisl dictum lacus, in egestas ligula nulla eu ex. Quisque vel justo congue, volutpat neque at, placerat nulla. Nam malesuada tellus sed justo pretium, eu vulputate mauris elementum. Donec elementum gravida ipsum id rhoncus.

Isolation

Picture

Cellular Lysis

Picture

DNA Digestion

Picture

Ligation

Modification

Picture

Transformation

Picture

PCR

Picture

Cell Culturing

Quantification

Picture

Antibiotic Resistance

Picture

Fluorescence

Picture

Cell Sorting