Difference between revisions of "Team:William and Mary/Parts"

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<cfheader name="X-XSS-Protection" value="1">
 
  
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<link rel="stylesheet" href="https://cdnjs.cloudflare.com/ajax/libs/font-awesome/4.7.0/css/font-awesome.min.css">
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<style>
  
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<div style='padding-top: 30px;'>
 
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<center>
 
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<img src="https://static.igem.org/mediawiki/2017/d/d0/T--William_and_Mary--parts5.jpeg" width = "100%"/>
 
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</center>
 
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table {
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float: right;
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  <div style='padding-top: 40px;padding-bottom: 40px;padding-left: 250px;padding-right: 250px; font-size: 22px; font-family: "Optima";'>This part is designed to easily facilitate appending the protein degradation tag (pdt)#3 tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.</div>
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</div>
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</center>
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table, th, td {
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  <div class="col-sm-9" style='padding-top: 80px; font-size: 30px;font-family: "Optima";'><span class="pull-right">mF-Lon</span></div>
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  <div class="col-sm-3" style='padding-left: 75px;'></div>
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<div class="row">
 
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  <div class="col-sm-9" style='padding-right:31px; padding-top: 5px; font-size: 30px;font-family: "Optima";'><span class="pull-right"><img src="https://static.igem.org/mediawiki/2017/4/4c/T--William_and_Mary--Separator1.jpeg" height = "18"/></span></div>
 
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  <div class="col-sm-3" style='padding-left: 75px;'></div>
 
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<div class="row">
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  <div class="col-sm-9" style='padding-top: 5px; padding-left: 75px; font-size: 22px; font-family: "Optima";'>Upcoming</div>
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padding: 1px;
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  <div class="col-sm-3" style='padding-right: 75px;'><IMG SRC="" width=220px></div>
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</head>
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<body>
  
 
<br></br>
 
<br></br>
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<center><div>Submitted Parts to the iGEM Registry</div></center>
 +
 
<br></br>
 
<br></br>
 +
 +
<!------UNS PDT----->
  
  
 
<div class="row">
 
<div class="row">
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<center>
 
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  <div class="col-sm-6" style='padding-top: 5px; padding-left: 75px; font-size: 30px; font-family: "Optima";'>Constitutive mf-Lon</div>
 
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</center>
 
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<center>
 
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  <div class="col-sm-6" style='padding-top: 5px; padding-right: 0px; font-size: 30px; font-family: "Optima";'>Inducible mf-Lon</div>
 
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</center>
 
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</div>
 
  
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<div class="container">
 
  
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<div class="col-sm-7">
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<div style='padding-left: 10%;'>test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test  </div>
 +
<div>
 +
<img src =""width="200px;"/>
 +
</div>
  
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  <div class="row">
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</div>
  
  
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  <div class="col-md-5">
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<div class="col-sm-4">
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  <div class="card add-animation-stopped animation-1">
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  <div class="icon">
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  <p style='font-size: 18px;'> This part is designed to easily facilitate appending the protein degradation tag (pdt)#3 tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.</p>
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−
  </div>
+
−
  <h3 style='font-family:Bookman; font-size: 25px;'><a href="https://2017.igem.org/Team:William_and_Mary/Measurement">
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−
                            </a></h3>
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  </div>
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  </div>
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<center>
 
<center>
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  <div class="col-md-2" style='padding-top: 2px;'>
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<table>
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  <div class="card add-animation-stopped animation-2">
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<tr>
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  <div class="icon">
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<th style='background-color: #BEB9C7;column-width: 50px;'><center>Part</center></th>
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  <a><img src="https://static.igem.org/mediawiki/2017/2/28/T--William_and_Mary--SpecialLine.jpeg" height= "800" width= "30"></a>
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<th style='background-color: #BEB9C7;column-width: 250px;'><center>Description</center></th>
−
  </div>
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</tr>
−
  <h3 style='font-family:Bookman; font-size: 25px;'><a href="https://2017.igem.org/Team:William_and_Mary/Results">  
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−
                            </a></h3>
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<tr>
−
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<td style='background-color: #9892A1;column-width: 50px; font-size: 17px;'><a href="http://parts.igem.org/Part:BBa_K2333401"> K2333401 </a></td>
−
  </div>
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<td style='background-color: #ECE7F2;column-width: 250px; font-size: 17px;'>UNS pdt #3A</td>
−
  </div>
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</tr>
 +
 
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 50px; font-size: 17px;'><a href="http://parts.igem.org/Part:BBa_K2333402"> K2333402 </a></td>
 +
<td style='background-color: #DED9E5;column-width: 250px; font-size: 17px;'>UNS pdt #3B</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 50px; font-size: 17px;'><a href="http://parts.igem.org/Part:BBa_K2333403"> K2333403 </a></td>
 +
<td style='background-color: #ECE7F2;column-width: 250px; font-size: 17px;'>UNS pdt #3C</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 50px; font-size: 17px;'><a href="http://parts.igem.org/Part:BBa_K2333404"> K2333404 </a></td>
 +
<td style='background-color: #DED9E5;column-width: 250px; font-size: 17px;'>UNS pdt #3D</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 50px; font-size: 17px;'><a href="http://parts.igem.org/Part:BBa_K2333405"> K2333405 </a></td>
 +
<td style='background-color: #ECE7F2;column-width: 250px; font-size: 17px;'>UNS pdt #3E</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 50px; font-size: 17px;'><a href="http://parts.igem.org/Part:BBa_K2333406"> K2333406 </a></td>
 +
<td style='background-color: #DED9E5;column-width: 250px; font-size: 17px;'>UNS pdt #3F</td>
 +
</tr>
 +
 
 +
</table>
 
</center>
 
</center>
−
  <div class="col-md-5">
 
−
  <div class="card add-animation-stopped animation-3">
 
−
  <div class="icon">
 
−
  <p style='font-size: 18px;'> This part is designed to easily facilitate appending the protein degradation tag (pdt)#3 tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.</p>
 
−
  </div>
 
−
  <h3 style='font-family:Bookman; font-size: 25px;'><a href="https://2017.igem.org/Team:William_and_Mary/Interlab">
 
−
                            </a></h3>  
 
−
  </div>
 
−
  </div>
 
 
</div>
 
</div>
  
 +
<div class="col-sm-1"></div>
  
  
 +
</div> <!----out of row-->
  
  
−
<br></br>
 
−
<div class="container">
 
  
 +
<!------GFP---->
  
−
  <div class="row">
 
  
 +
<div class="row">
  
−
  <div class="col-md-2">
+
 
−
  <div class="card add-animation-stopped animation-1">
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<div class="col-sm-7">
−
  <div class="icon">
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<div style='padding-left: 10%;'>test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test  </div>
−
  <a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2333401'><img src="https://static.igem.org/mediawiki/2017/d/d3/T--William_and_Mary--PDT3.jpeg" height= "120" width= "200"></a>
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−
  </div>
+
−
  <h3 style='font-family:Bookman; font-size: 25px;'><a href="https://2017.igem.org/Team:William_and_Mary/Measurement">
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−
                            </a></h3>
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−
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−
  </div>
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−
  </div>
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−
  <div class="col-md-2" style='padding-top: 2px;'>
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−
  <div class="card add-animation-stopped animation-2">
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−
  <div class="icon">
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−
  <a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2333402'><img src="https://static.igem.org/mediawiki/2017/6/67/T--William_and_Mary--PDT3a.jpeg" height= "120" width= "200"></a>
+
−
  </div>
+
−
  <h3 style='font-family:Bookman; font-size: 25px;'><a href="https://2017.igem.org/Team:William_and_Mary/Results">
+
−
                            </a></h3>
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−
+
−
  </div>
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−
  </div>
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−
  <div class="col-md-2">
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−
  <div class="card add-animation-stopped animation-3">
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−
  <div class="icon">
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  <a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2333403'><img src="https://static.igem.org/mediawiki/2017/5/5e/T--William_and_Mary--PDT3b.jpeg" height= "120" width= "200"></a>
+
−
  </div>
+
−
  <h3 style='font-family:Bookman; font-size: 25px;'><a href="https://2017.igem.org/Team:William_and_Mary/Interlab">
+
−
                            </a></h3>  
+
−
  </div>
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−
  </div>
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−
  <div class="col-md-2">
+
−
  <div class="card add-animation-stopped animation-4">
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−
  <div class="icon">
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−
  <a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2333404'><img src="https://static.igem.org/mediawiki/2017/3/38/T--William_and_Mary--PDT3c.jpeg" height= "120" width= "200"></a>
+
−
  </div>
+
−
<h3 style='font-family:Bookman; font-size: 25px;'>
+
−
<a href="https://2017.igem.org/Team:William_and_Mary/Medal_Requirements">
+
−
                          </a></h3>
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−
  </div>
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−
  </div>
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−
  <div class="col-md-2">
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−
  <div class="card add-animation-stopped animation-4">
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−
  <div class="icon">
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  <a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2333405'><img src="https://static.igem.org/mediawiki/2017/3/3d/T--William_and_Mary--PDT3d.jpeg" height= "120" width= "200"></a>
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−
  </div>
+
−
<h3 style='font-family:Bookman; font-size: 25px;'>
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−
<a href="https://2017.igem.org/Team:William_and_Mary/Medal_Requirements">
+
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                          </a></h3>
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  </div>
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  </div>
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  <div class="col-md-2">
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−
  <div class="card add-animation-stopped animation-4">
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−
  <div class="icon">
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−
  <a href='http://parts.igem.org/wiki/index.php?title=Part:BBa_K2333406'><img src="https://static.igem.org/mediawiki/2017/5/5c/T--William_and_Mary--PDT3e.jpeg" height= "120" width= "200"></a>
+
−
  </div>
+
−
<h3 style='font-family:Bookman; font-size: 25px;'>
+
−
<a href="https://2017.igem.org/Team:William_and_Mary/Medal_Requirements">
+
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                          </a></h3>
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−
  </div>
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−
  </div>
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−
  </div>
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−
  </div>
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−
  </div>
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<div>
 
<div>
 +
<img src =""width="200px;"/>
 +
</div>
  
 +
</div>
  
−
<center> For a full list of all parts submitted to the iGEM registry, <a href ="https://2017.igem.org/Team:William_and_Mary/PartsTable"> click here </a>.</center>
 
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</html>
 
  
 +
<div class="col-sm-4">
 +
<center>
 +
<table>
 +
<tr>
 +
<th style='background-color: #BEB9C7;column-width: 50px;'><center>Part</center></th>
 +
<th style='background-color: #BEB9C7;column-width: 250px;'><center>Description</center></th>
 +
</tr>
  
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 50px; font-size: 17px;'><a href="http://parts.igem.org/Part:BBa_K2066059"> K2066059 </a></td>
 +
<td style='background-color: #ECE7F2;column-width: 250px; font-size: 17px;'>J23100 GFP</td>
 +
</tr>
  
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 50px; font-size: 17px;'><a href="http://parts.igem.org/Part:BBa_K2333407"> K2333407 </a></td>
 +
<td style='background-color: #DED9E5;column-width: 250px; font-size: 17px;'>J23100 GFP pdt #3A</td>
 +
</tr>
  
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 50px; font-size: 17px;'><a href="http://parts.igem.org/Part:BBa_K2333408"> K2333408 </a></td>
 +
<td style='background-color: #ECE7F2;column-width: 250px; font-size: 17px;'>J23100 GFP pdt #3B</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 50px; font-size: 17px;'><a href="http://parts.igem.org/Part:BBa_K2333409"> K2333409 </a></td>
 +
<td style='background-color: #DED9E5;column-width: 250px; font-size: 17px;'>J23100 GFP pdt #3C</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 50px; font-size: 17px;'><a href="http://parts.igem.org/Part:BBa_K2333410"> K2333410 </a></td>
 +
<td style='background-color: #ECE7F2;column-width: 250px; font-size: 17px;'>J23100 GFP pdt #3D</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 50px; font-size: 17px;'><a href="http://parts.igem.org/Part:BBa_K2333411"> K2333411 </a></td>
 +
<td style='background-color: #DED9E5;column-width: 250px; font-size: 17px;'>J23100 GFP pdt #3E</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 50px; font-size: 17px;'><a href="http://parts.igem.org/Part:BBa_K2333412"> K2333412 </a></td>
 +
<td style='background-color: #DED9E5;column-width: 250px; font-size: 17px;'>J23100 GFP pdt #3F</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 50px; font-size: 17px;'><a href="http://parts.igem.org/Part:BBa_K2333420"> K2333420 </a></td>
 +
<td style='background-color: #ECE7F2;column-width: 250px; font-size: 17px;'>aTC-inducible GFP</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 50px; font-size: 17px;'><a href="http://parts.igem.org/Part:BBa_K2333421"> K2333421 </a></td>
 +
<td style='background-color: #DED9E5;column-width: 250px; font-size: 17px;'>aTC-inducible GFP pdt #3A</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 50px; font-size: 17px;'><a href="http://parts.igem.org/Part:BBa_K2333422"> K2333422 </a></td>
 +
<td style='background-color: #ECE7F2;column-width: 250px; font-size: 17px;'>aTC-inducible GFP pdt #3B</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 50px; font-size: 17px;'><a href="http://parts.igem.org/Part:BBa_K2333423"> K2333423 </a></td>
 +
<td style='background-color: #DED9E5;column-width: 250px; font-size: 17px;'>aTC-inducible GFP pdt #3C</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 50px; font-size: 17px;'><a href="http://parts.igem.org/Part:BBa_K2333424"> K2333424 </a></td>
 +
<td style='background-color: #ECE7F2;column-width: 250px; font-size: 17px;'>aTC-inducible GFP pdt #3D</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 50px; font-size: 17px;'><a href="http://parts.igem.org/Part:BBa_K2333425"> K2333425 </a></td>
 +
<td style='background-color: #DED9E5;column-width: 250px; font-size: 17px;'>aTC-inducible GFP pdt #3E</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 50px; font-size: 17px;'><a href="http://parts.igem.org/Part:BBa_K2333426"> K2333426 </a></td>
 +
<td style='background-color: #DED9E5;column-width: 250px; font-size: 17px;'>aTC-inducible GFP pdt #3F</td>
 +
</tr>
 +
 +
 +
</table>
 +
 +
</center>
 +
</div>
 +
 +
<div class="col-sm-1"></div>
 +
 +
 +
</div> <!----out of row-->
 +
 +
 +
</body>
 +
 +
</html>
  
 +
{{Team:William_and_Mary/JS_SOURCE}}
 
{{Team:William_and_Mary/FOOTER_SMALL}}
 
{{Team:William_and_Mary/FOOTER_SMALL}}

Revision as of 23:51, 28 October 2017



Submitted Parts to the iGEM Registry


test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test
Part
Description
K2333401 UNS pdt #3A
K2333402 UNS pdt #3B
K2333403 UNS pdt #3C
K2333404 UNS pdt #3D
K2333405 UNS pdt #3E
K2333406 UNS pdt #3F
test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test test
Part
Description
K2066059 J23100 GFP
K2333407 J23100 GFP pdt #3A
K2333408 J23100 GFP pdt #3B
K2333409 J23100 GFP pdt #3C
K2333410 J23100 GFP pdt #3D
K2333411 J23100 GFP pdt #3E
K2333412 J23100 GFP pdt #3F
K2333420 aTC-inducible GFP
K2333421 aTC-inducible GFP pdt #3A
K2333422 aTC-inducible GFP pdt #3B
K2333423 aTC-inducible GFP pdt #3C
K2333424 aTC-inducible GFP pdt #3D
K2333425 aTC-inducible GFP pdt #3E
K2333426 aTC-inducible GFP pdt #3F