Difference between revisions of "Team:TU Dresden/Experiments"
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<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/e/e0/T--TU_Dresden--M_Cloning.pdf">Cloning procedure</a> | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/e/e0/T--TU_Dresden--M_Cloning.pdf">Cloning procedure</a> | ||
| − | <a class="pdf-resources" href="https://static.igem.org/mediawiki/2017/6/6d/T--TU_Dresden--M_TransformationCaCl.pdf">Transformation CaCl method</a> | + | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/6/6d/T--TU_Dresden--M_TransformationCaCl.pdf">Transformation CaCl method</a> |
| − | <a class="pdf-resources" href="https://static.igem.org/mediawiki/2017/0/09/T--TU_Dresden--M_OneTaqPCR.pdf">PCR with One Taq Polymerase</a> | + | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/0/09/T--TU_Dresden--M_OneTaqPCR.pdf">PCR with One Taq Polymerase</a> |
| − | <a class="pdf-resources" href="https://static.igem.org/mediawiki/2017/1/17/T--TU_Dresden--M_Q5PCR.pdf">PCR with Q5 Polymerase</a> | + | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/1/17/T--TU_Dresden--M_Q5PCR.pdf">PCR with Q5 Polymerase</a> |
| − | <a class="pdf-resources" href="https://static.igem.org/mediawiki/2017/b/bf/T--TU_Dresden--M_LFH.pdf">Long flanking homology PCR</a> | + | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/b/bf/T--TU_Dresden--M_LFH.pdf">Long flanking homology PCR</a> |
<p> | <p> | ||
<hr> | <hr> | ||
<h2> Working with <i>Bacillus subtilis</i></h2> | <h2> Working with <i>Bacillus subtilis</i></h2> | ||
| − | <a class="pdf-resources" "https://static.igem.org/mediawiki/2017/3/3c/T--TU_Dresden--M_DNA_Isolation.pdf">DNA isolation (for PCR)</a> | + | <a class="pdf-resources" target="_blank" href= "https://static.igem.org/mediawiki/2017/3/3c/T--TU_Dresden--M_DNA_Isolation.pdf">DNA isolation (for PCR)</a> |
| − | <a class="pdf-resources" href="https://static.igem.org/mediawiki/2017/f/f7/T--TU_Dresden--M_IsolationDNATrafo.pdf">DNA isolation (for transformation)</a> | + | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/f/f7/T--TU_Dresden--M_IsolationDNATrafo.pdf">DNA isolation (for transformation)</a> |
| − | <a class="pdf-resources" href="https://static.igem.org/mediawiki/2017/5/5e/T--TU_Dresden--M_Transformation_Bacillus.pdf">Transformation of <i>B. subtilis </i></a> | + | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/5/5e/T--TU_Dresden--M_Transformation_Bacillus.pdf">Transformation of <i>B. subtilis </i></a> |
<p> | <p> | ||
<hr> | <hr> | ||
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<h2>Media and Solutions </h2> | <h2>Media and Solutions </h2> | ||
| − | <a class="pdf-resources" "https://static.igem.org/mediawiki/2017/9/9d/T--TU_Dresden--M_Media_Antibiotics.pdf">Media, Solutions & Antibiotics</a> | + | <a class="pdf-resources" target="_blank" href= "https://static.igem.org/mediawiki/2017/9/9d/T--TU_Dresden--M_Media_Antibiotics.pdf">Media, Solutions & Antibiotics</a> |
| − | <a class="pdf-resources" href="https://static.igem.org/mediawiki/2017/f/f2/T--TU_Dresden--M_JensensMedia.pdf">Jensen's Medium</a> | + | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/f/f2/T--TU_Dresden--M_JensensMedia.pdf">Jensen's Medium</a> |
| − | <a class="pdf-resources" href="https://static.igem.org/mediawiki/2017/4/45/T--TU_Dresden--M_MCSE.pdf">MCSE Medium</a> | + | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/4/45/T--TU_Dresden--M_MCSE.pdf">MCSE Medium</a> |
<p> | <p> | ||
<hr> | <hr> | ||
| Line 44: | Line 44: | ||
<h2>Primer</h2> | <h2>Primer</h2> | ||
| − | <a class="pdf-resources" href="https://static.igem.org/mediawiki/2017/b/b9/T--TU_Dresden--M_Primerlist.pdf">Primerlist general</a> | + | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/b/b9/T--TU_Dresden--M_Primerlist.pdf">Primerlist general</a> |
| − | <a class="pdf-resources" href="https://static.igem.org/mediawiki/2017/9/9b/T--TU_Dresden--M_Primerlist_Signalpeptides.pdf">Primerlist Signalpeptides</a> | + | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/9/9b/T--TU_Dresden--M_Primerlist_Signalpeptides.pdf">Primerlist Signalpeptides</a> |
<p> | <p> | ||
<hr> | <hr> | ||
<h2>Assays</h2> | <h2>Assays</h2> | ||
| − | <a class="pdf-resources" href="https://static.igem.org/mediawiki/2017/0/07/T--TU_Dresden--M_DiscDiffusion.pdf">Disc Diffussion Assay</a> | + | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/0/07/T--TU_Dresden--M_DiscDiffusion.pdf">Disc Diffussion Assay</a> |
| − | <a class="pdf-resources" href="https://static.igem.org/mediawiki/2017/7/71/T--TU_Dresden--M_PR_Luminescence.pdf">Plate Reader Assay</a> | + | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/7/71/T--TU_Dresden--M_PR_Luminescence.pdf">Plate Reader Assay</a> |
<p> | <p> | ||
<hr> | <hr> | ||
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<h2>Protein work</h2> | <h2>Protein work</h2> | ||
| − | <a class="pdf-resources" href="https://static.igem.org/mediawiki/2017/8/81/T--TU_Dresden--M_Proteins.pdf">His-Tag purification with agarose beads</a> | + | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/8/81/T--TU_Dresden--M_Proteins.pdf">His-Tag purification with agarose beads</a> |
Revision as of 09:48, 29 October 2017
