Difference between revisions of "Team:Heidelberg/Collaborations"
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Mutagenesis plasmids are crucial to enable rapid mutation that makes continuous evolution possible in short time scales. The mutagenesis plasmids we used have a \(P_{BAD}\) promotor that is arabinose inducible but suppressed by glucose<x-ref>RN159</x-ref>. Consequently controlling the glucose concentration is important in order to have a strong induction of the mutagenesis plasmids that results in a high mutation rate, which leads to a larger covered sequence space. This model uses ordinaray differential equations (ODE) to model both the glucose and the <i>E. coli</i> concentration, assuming both are independend of each other. This is plausible because <a href="https://2017.igem.org/Team:Heidelberg/Experiments#medium">the medium used in the experiments</a> contained other carbon sources than glucose. The glucose consumption rate per <i>E. coli</i> is assumed to be independent of the glucose concentration. | Mutagenesis plasmids are crucial to enable rapid mutation that makes continuous evolution possible in short time scales. The mutagenesis plasmids we used have a \(P_{BAD}\) promotor that is arabinose inducible but suppressed by glucose<x-ref>RN159</x-ref>. Consequently controlling the glucose concentration is important in order to have a strong induction of the mutagenesis plasmids that results in a high mutation rate, which leads to a larger covered sequence space. This model uses ordinaray differential equations (ODE) to model both the glucose and the <i>E. coli</i> concentration, assuming both are independend of each other. This is plausible because <a href="https://2017.igem.org/Team:Heidelberg/Experiments#medium">the medium used in the experiments</a> contained other carbon sources than glucose. The glucose consumption rate per <i>E. coli</i> is assumed to be independent of the glucose concentration. | ||
Revision as of 21:48, 29 October 2017
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