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| − | <h2 style='padding-top: 40px; font-family: "Verlag-Book"; font-size: 50px;'>
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| − | Interlab
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| − | <h3 style='padding-top: 50px; padding-bottom: 50px;'>Advisors</h3>
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| − | <div class="description">
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| − | <p class='large' style="padding-left:40px; text-indent: 50px;">
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| − | We participated in the Interlab Measurement Study using Flow Cytometry analysis on a FACS (Fluorescence-Activated Cell Sorter)
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| − | machine following the protocol provided by iGEM. Our results are presented here:
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| − | <img src="https://static.igem.org/mediawiki/2016/c/c7/Updated_interlab_160902_data.svg" width="600px">
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| − | <p class='large' style="padding-left:40px; text-indent: 50px; padding-top: 40px;">
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| − | In addition to participating in the Interlab Study, we also wanted to determine if there would be a noticeable difference
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| − | between measurements taken at Midlog and measurements taken on those same samples at Saturation.
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| − | <img src="https://static.igem.org/mediawiki/2016/a/af/Midlog_vs_saturated.svg" width="600px">
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| − | <p class='large' style="padding-left:40px; text-indent: 50px; padding-top: 40px;">
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| − | We noticed that although there were differences between the midlog and saturation measurements, they were not very drastic
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| − | across the different devices when looking at bulk population-level mean measurements, with the exception of the negative control.
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| − | </p>
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| − | <p class='large' style="padding-left:40px; text-indent: 50px;">
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| − | However, because we measured the devices with flow cytometry, we were additionally able to assess the changes in population
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| − | heterogeneity which occur with between midlog phase and saturation phase. We found that the midlog populations tended to be more
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| − | unimodal than the saturation populations.
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| − | <p class='large' style="padding-left:40px; text-align: center !important; padding-top: 500px; color: #A9A9A9; font-size: .75em !important;">
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| − | Fig. 3: Representative single-cell histograms of absolute fluorescence levels for the same sample at midlog phase vs. at saturation phase
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| − | </p>
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| − | <p class='large' style="padding-left:40px; text-indent: 50px; padding-top: 40px;">
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| − | A possible explanation for this phenomenon is that the antibiotic selection for the reporter construct may weaken over time,
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| − | allowing subpopulations of cells to develop which have varying metabolic emphases on the reporter construct.
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| − | </p>
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| − | <p class='large' style="padding-left:40px; text-indent: 50px;">
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| − | All of the FACS plots from our Interlab study are available upon request.
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| | {{Team:William_and_Mary/JS_SOURCE2}} | | {{Team:William_and_Mary/JS_SOURCE2}} |
| | {{Team:William_and_Mary/FOOTER_SMALL}} | | {{Team:William_and_Mary/FOOTER_SMALL}} |
