Difference between revisions of "Team:Heidelberg/Toolbox"

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                                             <h2 style="text-align: center; color:#6698BE !important; font-weight: 400 !important ">Plasmid</h2>
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<div class="content" style="padding: 10px 20px 10px 20px !important"><i>1. First, choose your preferred backbone from the pull down menu. We provide three different origins of replication that differ in their copy number and therefore affect the selection stringency, your AP will have. Be aware that the origin of replication should be compatible to the origin of replication of all other plasmids you plan to use. In most of the cases it is best to combine your origin of replication with an ampicillin resistance, but we provide other antibiotic resistances for special applications as well.</i></div>
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<div class="content" style="padding: 10px 20px 10px 20px !important"><i>2. Second, decide which RBS you need for control of geneIII translation. We offer six different RBS with different strengths. For further information look...</i></div>
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<div class="content" style="padding: 10px 20px 10px 20px !important"><i>3. Next, decide, which reporter you would like to choose. A set of different fluorescent and luminescent reporters is available</i></div>
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                                             Choose on of our sequences or upload your own
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                                             <div class="content" style="padding: 10px 20px 10px 20px !important"><i>4. When all standard parts are defined, the promoter and associated regulatory sequences must be set. Decide between the given options. Enter either an own sequence (as text or upload the file) or use one of the provided standard promoters that were already used by our team. Notice that the length of this part should not be below 200 bp. Ensure, that your sequence does not include a RBS.</i></div>
 
                                              
 
                                              
 
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                                          <div class="content" style="padding: 10px 20px 10px 20px !important"><i>5. Finally, decide which additional gene you would like to express on your AP. Most circuits that are used in PACE, need at least one additional protein, like split-proteins, chaperone or interacting factors. Again, it is possible to enter the desired sequence or choose one of the provided genes. Make sure, you enter a whole expression cassette, with promter, RBS, CDS and terminator.</i></div>
 
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Revision as of 13:13, 31 October 2017

Welcome to our
Toolbox Guide!

To enable rapid design and simple cloning of APs, we created a software tool, so that cloning can easily be planned. All parts that can be chosen are available in pSB1C3 backbones from the registry. Just follow the instructions and create your own fully customized AP.

Are you ready to PREDCEL?

First question

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Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua.

Second question

Plasmid

1. First, choose your preferred backbone from the pull down menu. We provide three different origins of replication that differ in their copy number and therefore affect the selection stringency, your AP will have. Be aware that the origin of replication should be compatible to the origin of replication of all other plasmids you plan to use. In most of the cases it is best to combine your origin of replication with an ampicillin resistance, but we provide other antibiotic resistances for special applications as well.
2. Second, decide which RBS you need for control of geneIII translation. We offer six different RBS with different strengths. For further information look...
3. Next, decide, which reporter you would like to choose. A set of different fluorescent and luminescent reporters is available

Resistance & Ori

RBS + geneIII

RBS + Reporter

Activator & Promoter

4. When all standard parts are defined, the promoter and associated regulatory sequences must be set. Decide between the given options. Enter either an own sequence (as text or upload the file) or use one of the provided standard promoters that were already used by our team. Notice that the length of this part should not be below 200 bp. Ensure, that your sequence does not include a RBS.

Additional sequence

5. Finally, decide which additional gene you would like to express on your AP. Most circuits that are used in PACE, need at least one additional protein, like split-proteins, chaperone or interacting factors. Again, it is possible to enter the desired sequence or choose one of the provided genes. Make sure, you enter a whole expression cassette, with promter, RBS, CDS and terminator.
Create Plasmid