Difference between revisions of "Team:William and Mary/Protocols"

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<center>  <div style = 'padding-right: 70px; padding-left:100px;' >Protocols </div> </center>
 
<center>  <div style = 'padding-right: 70px; padding-left:100px;' >Protocols </div> </center>
  
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<div style = 'padding-right: 70px; padding-left: 70px;' >Fluorescence-activated cell sorting (FACS) </div>  
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<div style = 'padding-right: 70px; padding-left: 70px;' >FLow Cytometry - We used the BioRad S3e Cell Sorter to carry out single-cell fluorescence measurements. We diluted our culture in 400ul of Phosphate Buffered Saline (PBS) adding 100ul of culture.For our measurements with inductions, we split and induced the cells with IPTG 6 hours after they were inoculated.Four hours after the cells were induced, we diluted the cells in order to get our ideal concentration of  We used FlowCal for downstream analysis and conversion to absolute fluorescence units.</div>  
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Revision as of 23:16, 31 October 2017


Protocols
FLow Cytometry - We used the BioRad S3e Cell Sorter to carry out single-cell fluorescence measurements. We diluted our culture in 400ul of Phosphate Buffered Saline (PBS) adding 100ul of culture.For our measurements with inductions, we split and induced the cells with IPTG 6 hours after they were inoculated.Four hours after the cells were induced, we diluted the cells in order to get our ideal concentration of We used FlowCal for downstream analysis and conversion to absolute fluorescence units.