Protocols

Cloning

Cloning procedure Transformation CaCl method PCR with One Taq Polymerase PCR with Q5 Polymerase Long flanking homology PCR SOP protocols for cloning with the Evaluation Vector and the Signal Peptide Toolbox

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Working with Bacillus subtilis

DNA isolation (for PCR) DNA isolation (for transformation) Transformation of B. subtilis

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Media and Solutions

Media, Solutions & Antibiotics Jensen's Medium MCSE Medium

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Primer

Primerlist general Primerlist Signalpeptides

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Assays

Disc Diffussion Assay Plate reader assay Fluorescence Assay

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Protein work

His-Tag purification with agarose beads Laemmli SDS-PAGE

Peptidosome methods

Peptidosome creation Encapsulation of B. subtilis Plate reader Wellscan Peptidosomes