Difference between revisions of "Team:Heidelberg/Materials"

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{{Heidelberg/templateus/Contentsection|{{Heidelberg/accpanel|#F8991D|{{Heidelberg/accord|antibiotics|{{Heidelberg/templateus/Tablebox||{{#tag:html|<table class="table table-bordered mdl-shadow--4dp" XSSCleaned="overflow-x: scroll !important">
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<th>Name</th> <th>Supplier</th> <th></th> </tr>
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<td>Carbenicillin dissodium salt</td> <td>Carl Roth</td> <td></td> </tr>
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<td>Amicillin sodium salt</td> <td>Carl Roth</td> <td></td> </tr>
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<td>Chloramphenicol</td> <td>Carl Roth</td> <td></td> </tr>
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<td>Kanamycin sulfate</td> <td>Sigma Aldrich</td> <td></td> </tr>
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<td>Tetracycline</td> <td>Sigma Aldrich</td> <td></td> </tr>
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<td>Spectinomycine</td> <td>Sigma Aldrich</td> <td></td> </tr>
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</tbody></table>
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{{Heidelberg/accord|enzymes|{{Heidelberg/templateus/Tablebox||{{#tag:html|<table class="table table-bordered mdl-shadow--4dp" XSSCleaned="overflow-x: scroll !important">
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<thead style="background-color: #005493 !important;"> <tr>
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<th>Name</th> <th>Supplier</th> <th></th> <th></th> <th></th> <th></th> <th></th> <th></th> <th></th> <th></th> <th></th> </tr>
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</thead><tbody> <tr>
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<td>BamI </td> <td>New England BioLabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
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<tr>
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<td>BamHI-HF</td> <td>New England BioLabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
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<tr>
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<td>BgIII </td> <td>New England BioLabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
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<tr>
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<td>BsaI-HF</td> <td>New England BioLabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
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<tr>
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<td>BsmBI </td> <td>New England BioLabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
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<tr>
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<td>DpnI </td> <td>New England BioLabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
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<tr>
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<td>EcoRI </td> <td>New England BioLabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
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<td>EcoRI-HF </td> <td>New England BioLabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
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<tr>
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<td>Esp3I</td> <td>Thermo Fisher Scientific</td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
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<tr>
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<td>HindIII-HF </td> <td>New England BioLabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
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<tr>
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<td>Hinfl, recombinant</td> <td>New England BioLabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
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<tr>
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<td>HpaII </td> <td>New England BioLabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
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<tr>
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<td>KpnI-HF </td> <td>New England BioLabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
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<tr>
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<td>MfeI-HF </td> <td>New England BioLabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
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<tr>
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<td>MlyI, recombinant</td> <td>New England BioLabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
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<tr>
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<td>NheI-HF </td> <td>New England BioLabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
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<tr>
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<td>NotI-HF </td> <td>New England BioLabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
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<tr>
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<td>PacI </td> <td>New England BioLabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
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<tr>
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<td>PstI-HF </td> <td>New England BioLabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
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<tr>
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<td>PvuI-HF </td> <td>New England BioLabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
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<tr>
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<td>SalI-HF </td> <td>New England BioLabs </td> <td></td> <td></td> <td></td> <td> </td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
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<tr>
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<td>Sau3AI</td> <td>New England BioLabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
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<tr>
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<td>SpeI-HF </td> <td>New England BioLabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
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<tr>
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<td>XbaI </td> <td>New England BioLabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
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</tbody></table>
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{{Heidelberg/accord|equipment|{{Heidelberg/templateus/Tablebox||{{#tag:html|<table class="table table-bordered mdl-shadow--4dp" XSSCleaned="overflow-x: scroll !important">
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<thead style="background-color: #005493 !important;"> <tr>
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<th>Equipment</th> <th>Name</th> <th>Manufacturer</th> <th></th> <th></th> </tr>
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</thead><tbody> <tr>
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<td></td> <td></td> <td></td> <td></td> <td></td> </tr>
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<tr>
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<td>Table Centrifuge </td> <td>Microfuge® 18 Centrifuge </td> <td>Beckman CoulterTM</td> <td></td> <td></td> </tr>
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<tr>
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<td>Table Centrifuge </td> <td>Microfuge® 22R Centrifuge </td> <td>Beckman CoulterTM</td> <td></td> <td></td> </tr>
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<tr>
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<td>Centrifuge </td> <td>Allegra X-12R </td> <td>Beckman CoulterTM</td> <td></td> <td></td> </tr>
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<tr>
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<td>Shaker </td> <td>VORTEXGENIE 2 </td> <td>Scientific Industries, SITM</td> <td></td> <td></td> </tr>
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<tr>
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<td>Heating plate (magnet stirrer)</td> <td>MR Hei-Standard </td> <td>Heidolph</td> <td></td> <td></td> </tr>
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<tr>
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<td>Heatblock </td> <td>QBD2</td> <td>Grant</td> <td></td> <td></td> </tr>
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<tr>
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<td>Heatblock </td> <td>QBD4 </td> <td>Grant</td> <td></td> <td></td> </tr>
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<tr>
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<td>Heatblock (shaking function) </td> <td>Thermomixer comfort </td> <td>Eppendorf</td> <td></td> <td></td> </tr>
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<tr>
 +
<td>PCR-Machine</td> <td>MyCycler thermo cycler</td> <td>BioRad</td> <td></td> <td></td> </tr>
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<tr>
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<td>PCR-Machine</td> <td>T100 Therma Cycler</td> <td>BioRad</td> <td></td> <td></td> </tr>
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<tr>
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<td>PCR-Machine</td> <td>Mastercycler nexus X2</td> <td>Eppendorf </td> <td></td> <td></td> </tr>
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<tr>
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<td>Gel documentation</td> <td>Transluminator </td> <td>Vilber Lourmat</td> <td></td> <td></td> </tr>
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<tr>
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<td>Scale (fine)</td> <td>PioneerTM PA114C </td> <td>OHAUS</td> <td></td> <td></td> </tr>
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<tr>
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<td>Scale</td> <td>PioneerTM PA4101C </td> <td>OHAUS</td> <td></td> <td></td> </tr>
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<tr>
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<td>Incubator</td> <td>KBW 400</td> <td>Binder</td> <td></td> <td></td> </tr>
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<tr>
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<td>Incubator/ Shaker</td> <td>ISF1-X</td> <td>Kuhner shaker</td> <td></td> <td></td> </tr>
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<tr>
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<td>Incubator / Shaker</td> <td>HT Multitron Version 2 </td> <td>INFORS</td> <td></td> <td></td> </tr>
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<tr>
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<td>Plate Reader </td> <td>Infinite M200 </td> <td>Tecan</td> <td></td> <td></td> </tr>
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<tr>
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<td>Plate Reader </td> <td>GloMax® Discover</td> <td>Promega</td> <td></td> <td></td> </tr>
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<tr>
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<td>UV/VIS Spectrometer </td> <td>Ultrospec 3300 pro </td> <td>Amersham Biosciences</td> <td></td> <td></td> </tr>
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<tr>
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<td>Microvulume spectrometer</td> <td>NanoPhotometer NP80</td> <td>Implen</td> <td></td> <td></td> </tr>
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<tr>
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<td>PH-Meter</td> <td>PH-Meter 765 Calimatic </td> <td>Knick</td> <td></td> <td></td> </tr>
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<tr>
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<td>Lyophilizer </td> <td>Gefriertrocknungsanlage ALPHA 1-2 LD Plus Best. Nr. 101521 </td> <td>Christ</td> <td></td> <td></td> </tr>
 +
<tr>
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<td>Sonificator</td> <td>Sonoplus Gm 2070 (2002)</td> <td>Bandelin</td> <td></td> <td></td> </tr>
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<tr>
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<td>Vacuum Manifold </td> <td>Qiavac 24 Plus</td> <td>Qiagen</td> <td></td> <td></td> </tr>
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<tr>
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<td>HPLC</td> <td>HPLC system with binary pump, VWD detector and autosampler</td> <td>Knauer</td> <td></td> <td></td> </tr>
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<tr>
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<td>Nucleic acid purification system</td> <td>QIAcube</td> <td>Qiagen</td> <td></td> <td></td> </tr>
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<tr>
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<td></td> <td></td> <td></td> <td></td> <td></td> </tr>
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<tr>
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<td></td> <td></td> <td></td> <td></td> <td></td> </tr>
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<tr>
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<td>PACE related equipment is specified here:</td> <td></td> <td></td> <td></td> <td></td> </tr>
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</tbody></table>
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<thead style="background-color: #005493 !important;"> <tr>
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<th>Name</th> <th>Supplier</th> <th></th> <th></th> </tr>
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</thead><tbody> <tr>
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<td>QIAquick® Gel Extraction Kit</td> <td>Qiagen </td> <td></td> <td></td> </tr>
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<tr>
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<td>QIAquick® PCR Purification Kit </td> <td>Qiagen </td> <td></td> <td></td> </tr>
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<tr>
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<td>QIAprep® Spin Miniprep Kit </td> <td>Qiagen </td> <td></td> <td></td> </tr>
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<tr>
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<td>DNA Clean & Concentrator™-5</td> <td>Zymo Research</td> <td></td> <td></td> </tr>
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<tr>
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<td>ZymoPURE™ Plasmid Miniprep Kit</td> <td>Zymo Research</td> <td></td> <td></td> </tr>
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<tr>
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<td>ZymoPURE™ Plasmid Midiprep Kit</td> <td>Zymo Research</td> <td></td> <td></td> </tr>
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<tr>
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<td></td> <td></td> <td></td> <td></td> </tr>
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<tr>
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<td></td> <td></td> <td></td> <td></td> </tr>
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<tr>
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<td></td> <td></td> <td></td> <td></td> </tr>
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<tr>
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<td></td> <td></td> <td></td> <td></td> </tr>
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<tr>
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<td>NEB iGEM Support Kit 2</td> <td>New England Biolabs</td> <td></td> <td></td> </tr>
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<tr>
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<td>NEB Quick Cloning Box-HF</td> <td>New England Biolabs</td> <td></td> <td></td> </tr>
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<tr>
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<td>HiScribe T7 Quick High Yield RNA Synthesis Kit</td> <td>New England Biolabs</td> <td></td> <td></td> </tr>
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<tr>
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<td>MinION Basic starter pack (1 MinION, 2 Flow cells, 1 Sequencing Kit, 1 Wash kit)</td> <td>Oxford Nanopore </td> <td></td> <td></td> </tr>
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</tbody></table>
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{{Heidelberg/accord|marks|{{Heidelberg/templateus/Tablebox||{{#tag:html|<table class="table table-bordered mdl-shadow--4dp" XSSCleaned="overflow-x: scroll !important">
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<thead style="background-color: #005493 !important;"> <tr>
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<th>Name</th> <th>Supplier</th> <th></th> <th></th> </tr>
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</thead><tbody> <tr>
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<td>Quick-Load 2-Log DNA Ladder (0.1-10.0 kb) </td> <td>New England BioLabs </td> <td></td> <td></td> </tr>
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<tr>
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<td>Quick-Load 1 kb DNA Ladder </td> <td>New England BioLabs </td> <td></td> <td></td> </tr>
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<tr>
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<td>100 bp DNA Ladder</td> <td>New England BioLabs </td> <td></td> <td></td> </tr>
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<tr>
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<td>Novex® Sharp Pre-stained Protein Standard </td> <td>Invitrogen </td> <td></td> <td></td> </tr>
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<tr>
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<td>SERVA Triple Color Protein Standard </td> <td>SERVA Electrophoresis </td> <td></td> <td></td> </tr>
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</tbody></table>
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{{Heidelberg/accord|media|{{Heidelberg/templateus/Tablebox||{{#tag:html|<table class="table table-bordered mdl-shadow--4dp" XSSCleaned="overflow-x: scroll !important">
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<thead style="background-color: #005493 !important;"> <tr>
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<th>Media</th> <th>Composition</th> <th></th> </tr>
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</thead><tbody> <tr>
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<td>LB medium (Luria/Miller für d-2,5 kg Best. Nr.: X968.3</td> <td>10 g NaCl, 10 g tryptone/ peptone, 5 g yeast extract in 1 L dH2O</td> <td></td> </tr>
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<tr>
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<td>2xYT medium für die Molekularb-2,5 kg Best. Nr.: X966.3</td> <td>5 g NaCl, 16 g tryptone/ peptone, 10 g yeast extract in 1 L dH2O</td> <td></td> </tr>
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<tr>
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<td>SOB medium</td> <td>0,5 % yeast extract, 2 % tryptone/peptone, 10 mM NaCl, 2.5 mM KCl, 10 mM MgCl2, 10 mM MgSO4</td> <td></td> </tr>
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<tr>
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<td>SOB medium</td> <td>SOB supplemented with 20 mM glucose</td> <td></td> </tr>
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<tr>
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<td>M9 minimal medium</td> <td></td> <td></td> </tr>
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</tbody></table>
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{{Heidelberg/accord|other enzymes|{{Heidelberg/templateus/Tablebox||{{#tag:html|<table class="table table-bordered mdl-shadow--4dp" XSSCleaned="overflow-x: scroll !important">
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<thead style="background-color: #005493 !important;"> <tr>
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<th>Name</th> <th>Supplier</th> <th></th> <th></th> <th></th> <th></th> <th></th> <th></th> </tr>
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</thead><tbody> <tr>
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<td>Gibson Assembly® Master Mix </td> <td>New England Biolabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
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<tr>
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<td>T4 DNA Ligase </td> <td>New England Biolabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
 +
<tr>
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<td>T7 RNA Polymerase</td> <td>New England Biolabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
 +
<tr>
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<td>T4 Polynucleotide Kinase (PNK)</td> <td>New England Biolabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
 +
<tr>
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<td>T4 RNA Ligase 1 (ssRNA Ligase)</td> <td>New England Biolabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
 +
<tr>
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<td>Alkaline Phosphatase (CIP)</td> <td>New England Biolabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
 +
<tr>
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<td>Shrimp Alkaline Phosphatase (rSAP)</td> <td>New England Biolabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
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</tbody></table>
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<thead style="background-color: #005493 !important;"> <tr>
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<th>Name</th> <th>Supplier</th> <th></th> <th></th> <th></th> <th></th> <th></th> <th></th> </tr>
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</thead><tbody> <tr>
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<td>OneTaq® Quick-Load® 2X Master Mix with Standard Buffer</td> <td>New England Biolabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
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<tr>
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<td>Phusion Flash High-Fidelity PCR Master Mix</td> <td>Thermo Fisher Scientific</td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
 +
<tr>
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<td>Q5® High-Fidelity DNA Polymerase 2X Master Mix</td> <td>New England Biolabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
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Revision as of 17:00, 1 November 2017

Notebook
Materials
antibiotics

Name Supplier
Carbenicillin dissodium salt Carl Roth
Amicillin sodium salt Carl Roth
Chloramphenicol Carl Roth
Kanamycin sulfate Sigma Aldrich
Tetracycline Sigma Aldrich
Spectinomycine Sigma Aldrich
enzymes

Name Supplier
BamI  New England BioLabs 
BamHI-HF New England BioLabs 
BgIII  New England BioLabs 
BsaI-HF New England BioLabs 
BsmBI  New England BioLabs 
DpnI  New England BioLabs 
EcoRI  New England BioLabs 
EcoRI-HF  New England BioLabs 
Esp3I Thermo Fisher Scientific
HindIII-HF  New England BioLabs 
Hinfl, recombinant New England BioLabs 
HpaII  New England BioLabs 
KpnI-HF  New England BioLabs 
MfeI-HF  New England BioLabs 
MlyI, recombinant New England BioLabs 
NheI-HF  New England BioLabs 
NotI-HF  New England BioLabs 
PacI  New England BioLabs 
PstI-HF  New England BioLabs 
PvuI-HF  New England BioLabs 
SalI-HF  New England BioLabs 
Sau3AI New England BioLabs 
SpeI-HF  New England BioLabs 
XbaI  New England BioLabs 
equipment

Equipment Name Manufacturer
Table Centrifuge  Microfuge® 18 Centrifuge  Beckman CoulterTM
Table Centrifuge  Microfuge® 22R Centrifuge  Beckman CoulterTM
Centrifuge  Allegra X-12R  Beckman CoulterTM
Shaker  VORTEXGENIE 2  Scientific Industries, SITM
Heating plate (magnet stirrer) MR Hei-Standard  Heidolph
Heatblock  QBD2 Grant
Heatblock  QBD4  Grant
Heatblock (shaking function)  Thermomixer comfort  Eppendorf
PCR-Machine MyCycler thermo cycler BioRad
PCR-Machine T100 Therma Cycler BioRad
PCR-Machine Mastercycler nexus X2 Eppendorf
Gel documentation Transluminator  Vilber Lourmat
Scale (fine) PioneerTM PA114C  OHAUS
Scale PioneerTM PA4101C  OHAUS
Incubator KBW 400 Binder
Incubator/ Shaker ISF1-X Kuhner shaker
Incubator / Shaker HT Multitron Version 2  INFORS
Plate Reader  Infinite M200  Tecan
Plate Reader  GloMax® Discover Promega
UV/VIS Spectrometer  Ultrospec 3300 pro  Amersham Biosciences
Microvulume spectrometer NanoPhotometer NP80 Implen
PH-Meter PH-Meter 765 Calimatic  Knick
Lyophilizer  Gefriertrocknungsanlage ALPHA 1-2 LD Plus Best. Nr. 101521  Christ
Sonificator Sonoplus Gm 2070 (2002) Bandelin
Vacuum Manifold  Qiavac 24 Plus Qiagen
HPLC HPLC system with binary pump, VWD detector and autosampler Knauer
Nucleic acid purification system QIAcube Qiagen
PACE related equipment is specified here:
kits

Name Supplier
QIAquick® Gel Extraction Kit Qiagen 
QIAquick® PCR Purification Kit Qiagen 
QIAprep® Spin Miniprep Kit Qiagen 
DNA Clean & Concentrator™-5 Zymo Research
ZymoPURE™ Plasmid Miniprep Kit Zymo Research
ZymoPURE™ Plasmid Midiprep Kit Zymo Research
NEB iGEM Support Kit 2 New England Biolabs
NEB Quick Cloning Box-HF New England Biolabs
HiScribe T7 Quick High Yield RNA Synthesis Kit New England Biolabs
MinION Basic starter pack (1 MinION, 2 Flow cells, 1 Sequencing Kit, 1 Wash kit) Oxford Nanopore
marks

Name Supplier
Quick-Load 2-Log DNA Ladder (0.1-10.0 kb)  New England BioLabs 
Quick-Load 1 kb DNA Ladder  New England BioLabs 
100 bp DNA Ladder New England BioLabs 
Novex® Sharp Pre-stained Protein Standard  Invitrogen 
SERVA Triple Color Protein Standard  SERVA Electrophoresis 
media

Media Composition
LB medium (Luria/Miller für d-2,5 kg Best. Nr.: X968.3 10 g NaCl, 10 g tryptone/ peptone, 5 g yeast extract in 1 L dH2O
2xYT medium für die Molekularb-2,5 kg Best. Nr.: X966.3 5 g NaCl, 16 g tryptone/ peptone, 10 g yeast extract in 1 L dH2O
SOB medium 0,5 % yeast extract, 2 % tryptone/peptone, 10 mM NaCl, 2.5 mM KCl, 10 mM MgCl2, 10 mM MgSO4
SOB medium SOB supplemented with 20 mM glucose
M9 minimal medium
other enzymes

Name Supplier
Gibson Assembly® Master Mix  New England Biolabs 
T4 DNA Ligase  New England Biolabs
T7 RNA Polymerase New England Biolabs
T4 Polynucleotide Kinase (PNK) New England Biolabs
T4 RNA Ligase 1 (ssRNA Ligase) New England Biolabs
Alkaline Phosphatase (CIP) New England Biolabs
Shrimp Alkaline Phosphatase (rSAP) New England Biolabs
polymerases

Name Supplier
OneTaq® Quick-Load® 2X Master Mix with Standard Buffer New England Biolabs 
Phusion Flash High-Fidelity PCR Master Mix Thermo Fisher Scientific
Q5® High-Fidelity DNA Polymerase 2X Master Mix New England Biolabs