Difference between revisions of "Team:William and Mary/Parts"
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</center> | </center> | ||
</div> | </div> | ||
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| + | <div class="container"> | ||
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| + | |||
| + | <div class="row"> | ||
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| + | |||
| + | <div class="col-md-5"> | ||
| + | <div class="card add-animation-stopped animation-1"> | ||
| + | <div class="icon"> | ||
| + | <p style='font-size: 18px;'> This part is designed to easily facilitate appending the protein degradation tag (pdt)#3 tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.</p> | ||
| + | </div> | ||
| + | <h3 style='font-family:Bookman; font-size: 25px;'><a href="https://2017.igem.org/Team:William_and_Mary/Measurement"> | ||
| + | </a></h3> | ||
| + | |||
| + | </div> | ||
| + | </div> | ||
| + | <center> | ||
| + | <div class="col-md-2" style='padding-top: 2px;'> | ||
| + | <div class="card add-animation-stopped animation-2"> | ||
| + | <div class="icon"> | ||
| + | <a><img src="https://static.igem.org/mediawiki/2017/2/28/T--William_and_Mary--SpecialLine.jpeg" height= "800" width= "30"></a> | ||
| + | </div> | ||
| + | <h3 style='font-family:Bookman; font-size: 25px;'><a href="https://2017.igem.org/Team:William_and_Mary/Results"> | ||
| + | </a></h3> | ||
| + | |||
| + | </div> | ||
| + | </div> | ||
| + | </center> | ||
| + | <div class="col-md-5"> | ||
| + | <div class="card add-animation-stopped animation-3"> | ||
| + | <div class="icon"> | ||
| + | <p style='font-size: 18px;'> This part is designed to easily facilitate appending the protein degradation tag (pdt)#3 tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.</p> | ||
| + | </div> | ||
| + | <h3 style='font-family:Bookman; font-size: 25px;'><a href="https://2017.igem.org/Team:William_and_Mary/Interlab"> | ||
| + | </a></h3> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | |||
| + | |||
Revision as of 21:39, 5 July 2017
This part is designed to easily facilitate appending the protein degradation tag (pdt)#3 tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.
This part is designed to easily facilitate appending the protein degradation tag (pdt)#3 tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.
