Latest revision as of 20:57, 1 November 2017

Interlab

1. Interlab Study

As a first step to contribute to this year's iGEM competition we decided to participate in iGEM's fourth International Interlaboratory Study along with many other teams from around the world. This study is organized by iGEM's measurement committee in an effort to establish standardized, reliable and repeatable measurement tools for the iGEM community and the synthetic biology community as a whole. This year's iGEM InterLab study is about establishing a standardized protocol for the measurement of GFP using a plate reader. To start things off we needed a plate reader that is qualified to measure GFP fluorescence. Namely, Tecan Infinite M200 Pro plate reader. Additionally, we needed competent E.coli DH5α. These were prepared from glycerol stocks. Together with the material iGEM had provided we were ready for work. Throughout our experiments, we tested 8 plasmids (2 controls and 6 test devices) by measuring the OD₆₀₀ and the fluorescence of the cells carrying the constructs. The workflow can be separated into four segments. The first segment is the transformation of all plasmids into competent DH5α cells.

1.1 Transformation of 8 test devices into competent DH5α cells

The transformation was successful for all the plasmids and resulted in a sufficient amount of colonies on all plates. Therefore, two colonies from each plate were picked and inoculated in LB medium containing chloramphenicol overnight for approximately 16-18 hours at 37°C and 220 rpm.

1.2 Measurement of LUDOX-HS40 OD600 Reference Point

The second segment is the measurement of the LUDOX-HS40 solution to obtain a ratiometric conversion factor which allows us to transform the absorbance data into a standard OD₆₀₀ measurement. This is necessary because plate readers, in general, do not have a 1 cm path length which means that measurements are volume dependent. By doing so, we obtained following data.

Table 1: With these replicates measured, we obtained a ratiometric conversion factor of 4.30 for our specific plate reader when measured 100 μL of the provided LUDOX-HS40 to water.

	LUDOX-HS40	H2O
Replicate 1	0,0543	0,0424
Replicate 2	0,0508	0,0413
Replicate 3	0,0515	0,0424
Replicate 4	0,051	0,042
Arith. Mean	0,0519	0,042
Corrected Abs600	0,009875
Reference OD600	0,0425
OD600/Abs600	4,303797

1.3 Graphing a Fluorescein fluorescence standard curve

In the third segment, we were instructed to measure a standard curve for fluorescence of Fluorescein which can be used to correct our cell-based readings to an equivalent Fluorescein concentration. Following the instructions, we could obtain following results for the serial dilution of Fluorescein in phosphate buffered saline.

Table 2:

uM Fluorescein	50,00	25	12,5	6,25	3,125	1,5625	0,7813	0,3906	0,1953	0,0977	0,0488	0
Replicate 1	43791	28969	17384	9562	5001	2591	1355	676	344	173	87	3
Replicate 2	44517	29931	17243	9266	5010	2575	1325	668	324	170	84	2
Replicate 3	42992	28755	17684	10262	5451	2851	1439	733	369	184	91	2
Replicate 4	42990	28734	17280	9617	5261	2667	1381	696	337	178	90	5
Arith. Mean	43573	29097	17398	9676,8	5180,8	2671	1375	693,25	343,5	176,25	88	3
Arith. Std.Dev.	733,96	565,88	199,95	419,51	216,75	126,53	48,415	28,999	18,912	6,1305	3,1623	1,4142

Table 3:

uM Fluorescein/a.u.	50,00	25	12,5	6,25	3,125	1,5625	0,7813	0,3906	0,1953	0,0977	0,0488
Mean uM Fluorescein/a.u.	0,0011	0,0009	0,0007	0,0006	0,0006	0,0006	0,0006	0,0006	0,0006	0,0006	0,0006
Mean of med-high levels:	0,0007

Figure 1.1:

Fluorescence is plotted against the Fluorescein concentration

Figure 1.2:

Fluorescence is plotted against the Fluorescein concentration on a logarithmic scale

These data give us an approximate linear regression line when plotted on a logarithmic scale and show an expected exponential course when plotted in a linear form.

1.4 Measurement of OD₆₀₀ and Fluorescence of Transformed Cells

In the last of the four segments, the OD₆₀₀ and the fluorescence of the transformed cells were measured according to the protocol after 0, 2, 4 and 6 hours. Measurements of all these time points gave us the following data and calculations which were conducted with the values we obtained from the standard curves and the reference point.

Table 4:

Fluorescence Raw Readings:										Abs600Raw Readings:
Hour 0:	Neg. Control	Pos. Control	Device 1	Device 2	Device 3	Device 4	Device 5	Device 6	LB + Chlor (blank)	Hour 0:	Neg. Control	Pos. Control	Device 1	Device 2	Device 3	Device 4	Device 5	Device 6	LB + Chlor (blank)
Colony 1, Replicate 1	228	262	294	371	227	275	249	230	221	Colony 1, Replicate 1	0,0473	0,0467	0,0478	0,0493	0,0483	0,0486	0,0474	0,0456	0,0435
Colony 1, Replicate 2	222	298	270	302	214	271	237	230	242	Colony 1, Replicate 2	0,0459	0,0466	0,0464	0,0468	0,0471	0,0474	0,0459	0,0442	0,043
Colony 1, Replicate 3	196	265	269	285	227	268	231	219	205	Colony 1, Replicate 3	0,05	0,0499	0,0502	0,0503	0,0518	0,0513	0,0502	0,0479	0,0473
Colony 1, Replicate 4	227	302	285	312	225	272	244	232	235	Colony 1, Replicate 4	0,061	0,0667	0,0691	0,0714	0,0725	0,0709	0,0596	0,0624	0,0412
Colony 2, Replicate 1	226	258	297	287	230	284	255	221	221	Colony 2, Replicate 1	0,0473	0,0471	0,0504	0,0484	0,0482	0,0482	0,0496	0,0482	0,0474
Colony 2, Replicate 2	217	277	301	311	221	283	244	244	231	Colony 2, Replicate 2	0,0469	0,0472	0,0492	0,0485	0,0473	0,047	0,0479	0,0474	0,0477
Colony 2, Replicate 3	213	261	287	285	222	276	236	225	234	Colony 2, Replicate 3	0,0512	0,0499	0,0563	0,0527	0,052	0,0523	0,0539	0,0533	0,0521
Colony 2, Replicate 4	223	267	294	303	224	293	249	238	243	Colony 2, Replicate 4	0,0604	0,0696	0,0686	0,0617	0,0719	0,0714	0,0608	0,0611	0,0646

Hour 2:	Neg. Control	Pos. Control	Device 1	Device 2	Device 3	Device 4	Device 5	Device 6	LB + Chlor (blank)	Hour 2:	Neg. Control	Pos. Control	Device 1	Device 2	Device 3	Device 4	Device 5	Device 6	LB + Chlor (blank)
Colony 1, Replicate 1	234	841	916	973	241	640	740	242	222	Colony 1, Replicate 1	0,0652	0,0568	0,0599	0,0613	0,068	0,0521	0,0742	0,075	0,0465
Colony 1, Replicate 2	229	850	864	923	234	608	641	239	239	Colony 1, Replicate 2	0,06	0,0539	0,0569	0,057	0,0632	0,0496	0,0671	0,0713	0,0455
Colony 1, Replicate 3	230	741	810	897	236	551	545	254	236	Colony 1, Replicate 3	0,0671	0,057	0,0626	0,0637	0,0671	0,0547	0,0698	0,0754	0,0515
Colony 1, Replicate 4	227	758	942	893	240	564	613	241	240	Colony 1, Replicate 4	0,081	0,0695	0,082	0,0799	0,0833	0,0674	0,0836	0,085	0,0409
Colony 2, Replicate 1	233	913	1016	1045	246	521	661	244	215	Colony 2, Replicate 1	0,0667	0,0602	0,062	0,065	0,0704	0,0499	0,0729	0,0774	0,0491
Colony 2, Replicate 2	224	784	878	893	243	702	642	233	254	Colony 2, Replicate 2	0,0608	0,0558	0,0568	0,0592	0,0654	0,052	0,068	0,0721	0,0452
Colony 2, Replicate 3	217	712	765	877	228	597	571	219	232	Colony 2, Replicate 3	0,0642	0,0614	0,0624	0,0669	0,0719	0,0584	0,0732	0,0676	0,053
Colony 2, Replicate 4	227	706	748	904	237	598	576	252	247	Colony 2, Replicate 4	0,0684	0,057	0,071	0,0803	0,0959	0,0653	0,0843	0,0848	0,0732

Hour 4:	Neg. Control	Pos. Control	Device 1	Device 2	Device 3	Device 4	Device 5	Device 6	LB + Chlor (blank)	Hour 4:	Neg. Control	Pos. Control	Device 1	Device 2	Device 3	Device 4	Device 5	Device 6	LB + Chlor (blank)
Colony 1, Replicate 1	265	3725	4330	4534	327	1619	1493	293	224	Colony 1, Replicate 1	0,1765	0,1449	0,1431	0,1661	0,1677	0,0692	0,1766	0,193	0,047
Colony 1, Replicate 2	269	3980	3936	4087	334	1489	1551	275	229	Colony 1, Replicate 2	0,1804	0,1515	0,1365	0,1585	0,1861	0,07	0,19	0,167	0,0469
Colony 1, Replicate 3	245	2579	3110	3080	300	1280	1257	276	238	Colony 1, Replicate 3	0,1321	0,1194	0,1199	0,132	0,1482	0,0666	0,1573	0,1578	0,0516
Colony 1, Replicate 4	247	2934	3253	3214	313	1543	1358	304	234	Colony 1, Replicate 4	0,1354	0,1348	0,1296	0,1441	0,1762	0,0885	0,164	0,189	0,0456
Colony 2, Replicate 1	269	3980	3936	4087	334	1489	1551	275	229	Colony 2, Replicate 1	0,3007	0,2746	0,2586	0,308	0,3246	0,339	0,3438	0,3446	0,0473
Colony 2, Replicate 2	253	2825	3064	3547	321	1619	1294	308	237	Colony 2, Replicate 2	0,1467	0,1192	0,1135	0,1373	0,1643	0,0698	0,151	0,1849	0,0453
Colony 2, Replicate 3	249	3397	3055	3125	309	1516	1238	276	239	Colony 2, Replicate 3	0,1374	0,1356	0,1199	0,1344	0,1453	0,0763	0,1532	0,1626	0,0536
Colony 2, Replicate 4	261	2585	3302	3124	322	1589	1285	292	246	Colony 2, Replicate 4	0,1423	0,1255	0,138	0,1527	0,1686	0,0765	0,1522	0,1622	0,064

Hour 6:	Neg. Control	Pos. Control	Device 1	Device 2	Device 3	Device 4	Device 5	Device 6	LB + Chlor (blank)	Hour 6:	Neg. Control	Pos. Control	Device 1	Device 2	Device 3	Device 4	Device 5	Device 6	LB + Chlor (blank)
Colony 1, Replicate 1	289	7046	9753	10223	429	4924	5031	328	223	Colony 1, Replicate 1	0,2911	0,2572	0,2697	0,2843	0,311	0,1557	0,1581	0,3271	0,0473
Colony 1, Replicate 2	290	7161	9621	9685	429	4659	4916	322	233	Colony 1, Replicate 2	0,2735	0,2467	0,2558	0,2596	0,2984	0,1439	0,1471	0,3082	0,0492
Colony 1, Replicate 3	284	6900	8983	9620	420	4563	4830	335	236	Colony 1, Replicate 3	0,2694	0,2542	0,2557	0,2671	0,2856	0,1464	0,1531	0,3093	0,052
Colony 1, Replicate 4	320	7664	10384	10759	441	5092	5472	341	239	Colony 1, Replicate 4	0,3314	0,2762	0,2711	0,2787	0,2999	0,1738	0,1763	0,2938	0,0401
Colony 2, Replicate 1	285	7246	9059	10726	445	2372	2368	332	229	Colony 2, Replicate 1	0,3007	0,2746	0,2586	0,308	0,3246	0,339	0,3438	0,3446	0,0473
Colony 2, Replicate 2	286	7418	10409	10079	442	2357	2321	329	232	Colony 2, Replicate 2	0,2853	0,2574	0,2783	0,2668	0,2989	0,3188	0,3134	0,3161	0,0513
Colony 2, Replicate 3	285	6939	9332	10013	439	2297	2299	336	232	Colony 2, Replicate 3	0,282	0,2546	0,2629	0,2691	0,3006	0,3064	0,3063	0,3094	0,0538
Colony 2, Replicate 4	294	7882	9938	10980	446	2523	2513	330	253	Colony 2, Replicate 4	0,2851	0,2633	0,2614	0,2668	0,2911	0,3239	0,336	0,3191	0,0469

Table 5:

Unit Scaling Factors:
OD600/Abs600	4,30
uM Fluorescein/a.u.	6,82E-04

Table 6:

Experimental Values:	Raw Abs600				Raw Fluorescence
Sample set:	Replicate 1	Replicate 2	Replicate 3	Replicate 4	Replicate 1	Replicate 2	Replicate 3	Replicate 4
Blank media	0,0435	0,043	0,0473	0,0412	221	242	205	235
Blank mean:	0,04375				225,75				OD - Background				Fluorescence - Background				uM Fluorescein / OD600				Summary Statistics
Hour 0:									Replicate 1	Replicate 2	Replicate 3	Replicate 4	Replicate 1	Replicate 2	Replicate 3	Replicate 4	Replicate 1	Replicate 2	Replicate 3	Replicate 4	Arith. Mean	Arith. Std.Dev.	Geo. Mean	Geo. Std. Dev.
Negative Control (Colony 1)	0,0473	0,0459	0,05	0,061	228	222	196	227	0,00355	0,00215	0,00625	0,01725	2,25	-3,75	-29,75	1,25	0,10	-0,28	-0,75	0,01	-0,23	0,39
Negative Control (Colony 2)	0,0473	0,0469	0,0512	0,0604	226	217	213	223	0,00355	0,00315	0,00745	0,01665	0,25	-8,75	-12,75	-2,75	0,01	-0,44	-0,27	-0,03	-0,18	0,21
Positive Control (Colony 1)	0,0467	0,0466	0,0499	0,0667	262	298	265	302	0,00295	0,00285	0,00615	0,02295	36,25	72,25	39,25	76,25	1,95	4,02	1,01	0,53	1,88	1,55	1,43	2,391
Positive Control (Colony 2)	0,0471	0,0472	0,0499	0,0696	258	277	261	267	0,00335	0,00345	0,00615	0,02585	32,25	51,25	35,25	41,25	1,53	2,36	0,91	0,25	1,26	0,90	0,95	2,628
Test Device 1: J23101.BCD2.E0040.B0015 (Colony 1)	0,0478	0,0464	0,0502	0,0691	294	270	269	285	0,00405	0,00265	0,00645	0,02535	68,25	44,25	43,25	59,25	2,67	2,65	1,06	0,37	1,69	1,16	1,29	2,555
Test Device 1: J23101.BCD2.E0040.B0015 (Colony 2)	0,0504	0,0492	0,0563	0,0686	297	301	287	294	0,00665	0,00545	0,01255	0,02485	71,25	75,25	61,25	68,25	1,70	2,19	0,77	0,44	1,27	0,81	1,06	2,094
Test Device 2: J23106.BCD2.E0040.B0015 (Colony 1)	0,0493	0,0468	0,0503	0,0714	371	302	285	312	0,00555	0,00305	0,00655	0,02765	145,25	76,25	59,25	86,25	4,15	3,96	1,43	0,49	2,51	1,83	1,85	2,736
Test Device 2: J23106.BCD2.E0040.B0015 (Colony 2)	0,0484	0,0485	0,0527	0,0617	287	311	285	303	0,00465	0,00475	0,00895	0,01795	61,25	85,25	59,25	77,25	2,09	2,85	1,05	0,68	1,67	0,99	1,44	1,912
Test Device 3: J23117.BCD2.E0040.B0015 (Colony 1)	0,0483	0,0471	0,0518	0,0725	227	214	227	225	0,00455	0,00335	0,00805	0,02875	1,25	-11,75	1,25	-0,75	0,04	-0,56	0,02	0,00	-0,12	0,29
Test Device 3: J23117.BCD2.E0040.B0015 (Colony 2)	0,0482	0,0473	0,052	0,0719	230	221	222	224	0,00445	0,00355	0,00825	0,02815	4,25	-4,75	-3,75	-1,75	0,15	-0,21	-0,07	-0,01	-0,04	0,15
Test Device 4: J23101+I13504 (Colony 1)	0,0486	0,0474	0,0513	0,0709	275	271	268	272	0,00485	0,00365	0,00755	0,02715	49,25	45,25	42,25	46,25	1,61	1,97	0,89	0,27	1,18	0,76	0,93	2,442
Test Device 4: J23101+I13504 (Colony 2)	0,0482	0,047	0,0523	0,0714	284	283	276	293	0,00445	0,00325	0,00855	0,02765	58,25	57,25	50,25	67,25	2,08	2,79	0,93	0,39	1,55	1,09	1,20	2,431
Test Device 5: J23106+I13504 (Colony 1)	0,0474	0,0459	0,0502	0,0596	249	237	231	244	0,00365	0,00215	0,00645	0,01585	23,25	11,25	5,25	18,25	1,01	0,83	0,13	0,18	0,54	0,45	0,37	2,840
Test Device 5: J23106+I13504 (Colony 2)	0,0496	0,0479	0,0539	0,0608	255	244	236	249	0,00585	0,00415	0,01015	0,01705	29,25	18,25	10,25	23,25	0,79	0,70	0,16	0,22	0,47	0,32	0,37	2,250
Test Device 6: J23117+I13504 (Colony 1)	0,0456	0,0442	0,0479	0,0624	230	230	219	232	0,00185	0,00045	0,00415	0,01865	4,25	4,25	-6,75	6,25	0,36	1,50	-0,26	0,05	0,41	0,77
Test Device 6: J23117+I13504 (Colony 2)	0,0482	0,0474	0,0533	0,0611	221	244	225	238	0,00445	0,00365	0,00955	0,01735	-4,75	18,25	-0,75	12,25	-0,17	0,79	-0,01	0,11	0,18	0,42
Hour 2:
Negative Control (Colony 1)	0,0652	0,06	0,0671	0,081	234	229	230	227	0,02145	0,01625	0,02335	0,03725	8,25	3,25	4,25	1,25	0,06	0,03	0,03	0,01	0,03	0,02	0,02	2,830
Negative Control (Colony 2)	0,0667	0,0608	0,0642	0,0684	233	224	217	227	0,02295	0,01705	0,02045	0,02465	7,25	-1,75	-8,75	1,25	0,05	-0,02	-0,07	0,01	-0,01	0,05
Positive Control (Colony 1)	0,0568	0,0539	0,057	0,0695	841	850	741	758	0,01305	0,01015	0,01325	0,02575	615,25	624,25	515,25	532,25	7,47	9,75	6,17	3,28	6,67	2,70	6,19	1,591
Positive Control (Colony 2)	0,0602	0,0558	0,0614	0,057	913	784	712	706	0,01645	0,01205	0,01765	0,01325	687,25	558,25	486,25	480,25	6,62	7,35	4,37	5,75	6,02	1,28	5,91	1,253
Test Device 1: J23101.BCD2.E0040.B0015 (Colony 1)	0,0599	0,0569	0,0626	0,082	916	864	810	942	0,01615	0,01315	0,01885	0,03825	690,25	638,25	584,25	716,25	6,78	7,70	4,91	2,97	5,59	2,09	5,25	1,529
Test Device 1: J23101.BCD2.E0040.B0015 (Colony 2)	0,062	0,0568	0,0624	0,071	1016	878	765	748	0,01825	0,01305	0,01865	0,02725	790,25	652,25	539,25	522,25	6,87	7,92	4,58	3,04	5,60	2,21	5,25	1,540
Test Device 2: J23106.BCD2.E0040.B0015 (Colony 1)	0,0613	0,057	0,0637	0,0799	973	923	897	893	0,01755	0,01325	0,01995	0,03615	747,25	697,25	671,25	667,25	6,75	8,34	5,33	2,93	5,84	2,30	5,45	1,572
Test Device 2: J23106.BCD2.E0040.B0015 (Colony 2)	0,065	0,0592	0,0669	0,0803	1045	893	877	904	0,02125	0,01545	0,02315	0,03655	819,25	667,25	651,25	678,25	6,11	6,85	4,46	2,94	5,09	1,75	4,84	1,460
Test Device 3: J23117.BCD2.E0040.B0015 (Colony 1)	0,068	0,0632	0,0671	0,0833	241	234	236	240	0,02425	0,01945	0,02335	0,03955	15,25	8,25	10,25	14,25	0,10	0,07	0,07	0,06	0,07	0,02	0,07	1,265
Test Device 3: J23117.BCD2.E0040.B0015 (Colony 2)	0,0704	0,0654	0,0719	0,0959	246	243	228	237	0,02665	0,02165	0,02815	0,05215	20,25	17,25	2,25	11,25	0,12	0,13	0,01	0,03	0,07	0,06	0,05	3,018
Test Device 4: J23101+I13504 (Colony 1)	0,0521	0,0496	0,0547	0,0674	640	608	551	564	0,00835	0,00585	0,01095	0,02365	414,25	382,25	325,25	338,25	7,87	10,36	4,71	2,27	6,30	3,55	5,43	1,950
Test Device 4: J23101+I13504 (Colony 2)	0,0499	0,052	0,0584	0,0653	521	702	597	598	0,00615	0,00825	0,01465	0,02155	295,25	476,25	371,25	372,25	7,61	9,15	4,02	2,74	5,88	3,00	5,26	1,751
Test Device 5: J23106+I13504 (Colony 1)	0,0742	0,0671	0,0698	0,0836	740	641	545	613	0,03045	0,02335	0,02605	0,03985	514,25	415,25	319,25	387,25	2,68	2,82	1,94	1,54	2,25	0,61	2,18	1,329
Test Device 5: J23106+I13504 (Colony 2)	0,0729	0,068	0,0732	0,0843	661	642	571	576	0,02915	0,02425	0,02945	0,04055	435,25	416,25	345,25	350,25	2,37	2,72	1,86	1,37	2,08	0,59	2,01	1,351
Test Device 6: J23117+I13504 (Colony 1)	0,075	0,0713	0,0754	0,085	242	239	254	241	0,03125	0,02755	0,03165	0,04125	16,25	13,25	28,25	15,25	0,08	0,08	0,14	0,06	0,09	0,04	0,08	1,448
Test Device 6: J23117+I13504 (Colony 2)	0,0774	0,0721	0,0676	0,0848	244	233	219	252	0,03365	0,02835	0,02385	0,04105	18,25	7,25	-6,75	26,25	0,09	0,04	-0,04	0,10	0,05	0,07
Hour 4:
Negative Control (Colony 1)	0,1765	0,1804	0,1321	0,1354	265	269	245	247	0,13275	0,13665	0,08835	0,09165	39,25	43,25	19,25	21,25	0,05	0,05	0,03	0,04	0,04	0,01	0,04	1,199
Negative Control (Colony 2)	0,3007	0,1467	0,1374	0,1423	269	253	249	261	0,25695	0,10295	0,09365	0,09855	43,25	27,25	23,25	35,25	0,03	0,04	0,04	0,06	0,04	0,01	0,04	1,363
Positive Control (Colony 1)	0,1449	0,1515	0,1194	0,1348	3725	3980	2579	2934	0,10115	0,10775	0,07565	0,09105	3499,25	3754,25	2353,25	2708,25	5,48	5,52	4,93	4,72	5,16	0,40	5,15	1,082
Positive Control (Colony 2)	0,2746	0,1192	0,1356	0,1255	3980	2825	3397	2585	0,23085	0,07545	0,09185	0,08175	3754,25	2599,25	3171,25	2359,25	2,58	5,46	5,47	4,58	4,52	1,36	4,33	1,428
Test Device 1: J23101.BCD2.E0040.B0015 (Colony 1)	0,1431	0,1365	0,1199	0,1296	4330	3936	3110	3253	0,09935	0,09275	0,07615	0,08585	4104,25	3710,25	2884,25	3027,25	6,55	6,34	6,01	5,59	6,12	0,42	6,11	1,072
Test Device 1: J23101.BCD2.E0040.B0015 (Colony 2)	0,2586	0,1135	0,1199	0,138	3936	3064	3055	3302	0,21485	0,06975	0,07615	0,09425	3710,25	2838,25	2829,25	3076,25	2,74	6,45	5,89	5,17	5,06	1,64	4,82	1,473
Test Device 2: J23106.BCD2.E0040.B0015 (Colony 1)	0,1661	0,1585	0,132	0,1441	4534	4087	3080	3214	0,12235	0,11475	0,08825	0,10035	4308,25	3861,25	2854,25	2988,25	5,58	5,33	5,13	4,72	5,19	0,36	5,18	1,074
Test Device 2: J23106.BCD2.E0040.B0015 (Colony 2)	0,308	0,1373	0,1344	0,1527	4087	3547	3125	3124	0,26425	0,09355	0,09065	0,10895	3861,25	3321,25	2899,25	2898,25	2,32	5,63	5,07	4,22	4,31	1,45	4,09	1,487
Test Device 3: J23117.BCD2.E0040.B0015 (Colony 1)	0,1677	0,1861	0,1482	0,1762	327	334	300	313	0,12395	0,14235	0,10445	0,13245	101,25	108,25	74,25	87,25	0,13	0,12	0,11	0,10	0,12	0,01	0,12	1,096
Test Device 3: J23117.BCD2.E0040.B0015 (Colony 2)	0,3246	0,1643	0,1453	0,1686	334	321	309	322	0,28085	0,12055	0,10155	0,12485	108,25	95,25	83,25	96,25	0,06	0,13	0,13	0,12	0,11	0,03	0,11	1,436
Test Device 4: J23101+I13504 (Colony 1)	0,0692	0,07	0,0666	0,0885	1619	1489	1280	1543	0,02545	0,02625	0,02285	0,04475	1393,25	1263,25	1054,25	1317,25	8,68	7,63	7,31	4,67	7,07	1,71	6,90	1,310
Test Device 4: J23101+I13504 (Colony 2)	0,339	0,0698	0,0763	0,0765	1489	1619	1516	1589	0,29525	0,02605	0,03255	0,03275	1263,25	1393,25	1290,25	1363,25	0,68	8,48	6,28	6,60	5,51	3,36	3,93	3,250
Test Device 5: J23106+I13504 (Colony 1)	0,1766	0,19	0,1573	0,164	1493	1551	1257	1358	0,13285	0,14625	0,11355	0,12025	1267,25	1325,25	1031,25	1132,25	1,51	1,44	1,44	1,49	1,47	0,04	1,47	1,026
Test Device 5: J23106+I13504 (Colony 2)	0,3438	0,151	0,1532	0,1522	1551	1294	1238	1285	0,30005	0,10725	0,10945	0,10845	1325,25	1068,25	1012,25	1059,25	0,70	1,58	1,47	1,55	1,32	0,42	1,26	1,480
Test Device 6: J23117+I13504 (Colony 1)	0,193	0,167	0,1578	0,189	293	275	276	304	0,14925	0,12325	0,11405	0,14525	67,25	49,25	50,25	78,25	0,07	0,06	0,07	0,09	0,07	0,01	0,07	1,133
Test Device 6: J23117+I13504 (Colony 2)	0,3446	0,1849	0,1626	0,1622	275	308	276	292	0,30085	0,14115	0,11885	0,11845	49,25	82,25	50,25	66,25	0,03	0,09	0,07	0,09	0,07	0,03	0,06	1,807
Hour 6:
Negative Control (Colony 1)	0,2911	0,2735	0,2694	0,3314	289	290	284	320	0,24735	0,22975	0,22565	0,28765	63,25	64,25	58,25	94,25	0,04	0,04	0,04	0,05	0,04	0,01	0,04	1,122
Negative Control (Colony 2)	0,3007	0,2853	0,282	0,2851	285	286	285	294	0,25695	0,24155	0,23825	0,24135	59,25	60,25	59,25	68,25	0,04	0,04	0,04	0,04	0,04	0,00	0,04	1,088
Positive Control (Colony 1)	0,2572	0,2467	0,2542	0,2762	7046	7161	6900	7664	0,21345	0,20295	0,21045	0,23245	6820,25	6935,25	6674,25	7438,25	5,07	5,42	5,03	5,07	5,15	0,18	5,14	1,035
Positive Control (Colony 2)	0,2746	0,2574	0,2546	0,2633	7246	7418	6939	7882	0,23085	0,21365	0,21085	0,21955	7020,25	7192,25	6713,25	7656,25	4,82	5,34	5,05	5,53	5,18	0,31	5,18	1,062
Test Device 1: J23101.BCD2.E0040.B0015 (Colony 1)	0,2697	0,2558	0,2557	0,2711	9753	9621	8983	10384	0,22595	0,21205	0,21195	0,22735	9527,25	9395,25	8757,25	10158,25	6,69	7,02	6,55	7,08	6,84	0,26	6,83	1,039
Test Device 1: J23101.BCD2.E0040.B0015 (Colony 2)	0,2586	0,2783	0,2629	0,2614	9059	10409	9332	9938	0,21485	0,23455	0,21915	0,21765	8833,25	10183,25	9106,25	9712,25	6,52	6,88	6,59	7,07	6,77	0,26	6,76	1,039
Test Device 2: J23106.BCD2.E0040.B0015 (Colony 1)	0,2843	0,2596	0,2671	0,2787	10223	9685	9620	10759	0,24055	0,21585	0,22335	0,23495	9997,25	9459,25	9394,25	10533,25	6,59	6,95	6,67	7,11	6,83	0,24	6,83	1,036
Test Device 2: J23106.BCD2.E0040.B0015 (Colony 2)	0,308	0,2668	0,2691	0,2668	10726	10079	10013	10980	0,26425	0,22305	0,22535	0,22305	10500,25	9853,25	9787,25	10754,25	6,30	7,00	6,89	7,64	6,96	0,55	6,94	1,082
Test Device 3: J23117.BCD2.E0040.B0015 (Colony 1)	0,311	0,2984	0,2856	0,2999	429	429	420	441	0,26725	0,25465	0,24185	0,25615	203,25	203,25	194,25	215,25	0,12	0,13	0,13	0,13	0,13	0,01	0,13	1,042
Test Device 3: J23117.BCD2.E0040.B0015 (Colony 2)	0,3246	0,2989	0,3006	0,2911	445	442	439	446	0,28085	0,25515	0,25685	0,24735	219,25	216,25	213,25	220,25	0,12	0,13	0,13	0,14	0,13	0,01	0,13	1,056
Test Device 4: J23101+I13504 (Colony 1)	0,1557	0,1439	0,1464	0,1738	4924	4659	4563	5092	0,11195	0,10015	0,10265	0,13005	4698,25	4433,25	4337,25	4866,25	6,65	7,02	6,70	5,93	6,58	0,46	6,56	1,074
Test Device 4: J23101+I13504 (Colony 2)	0,339	0,3188	0,3064	0,3239	2372	2357	2297	2523	0,29525	0,27505	0,26265	0,28015	2146,25	2131,25	2071,25	2297,25	1,15	1,23	1,25	1,30	1,23	0,06	1,23	1,052
Test Device 5: J23106+I13504 (Colony 1)	0,1581	0,1471	0,1531	0,1763	5031	4916	4830	5472	0,11435	0,10335	0,10935	0,13255	4805,25	4690,25	4604,25	5246,25	6,66	7,20	6,68	6,28	6,70	0,38	6,69	1,058
Test Device 5: J23106+I13504 (Colony 2)	0,3438	0,3134	0,3063	0,336	2368	2321	2299	2513	0,30005	0,26965	0,26255	0,29225	2142,25	2095,25	2073,25	2287,25	1,13	1,23	1,25	1,24	1,21	0,06	1,21	1,048
Test Device 6: J23117+I13504 (Colony 1)	0,3271	0,3082	0,3093	0,2938	328	322	335	341	0,28335	0,26445	0,26555	0,25005	102,25	96,25	109,25	115,25	0,06	0,06	0,07	0,07	0,06	0,01	0,06	1,123
Test Device 6: J23117+I13504 (Colony 2)	0,3446	0,3161	0,3094	0,3191	332	329	336	330	0,30085	0,27235	0,26565	0,27535	106,25	103,25	110,25	104,25	0,06	0,06	0,07	0,06	0,06	0,00	0,06	1,068

2. Discussion

The protocol was easy to follow and the constructs were nicely expressed which allowed reliable and comparable measurements. In the process of obtaining transformed DH5α cells we did not encounter any problems with transformation as bacteria showed exceptional growth. The specific ratiometric conversion factor of our plate reader could be determined without further adjustments using the LUDOX-HS40 solution. When measuring the Fluorescein standard curve on a logarithmic scale the data points showed a linear distribution. The final step, the measurement of the fluorescence emission and optical density of the cells, resulted in the raw data shown in table 1.5. To make this data visually accessible the mean values for OD and fluorescence were calculated and plotted against their respective time points which are seen in the following graphs.

Figure 1.3:

Calculated means from both replicates for the OD₆₀₀ after each time point

Figure 1.4:

Calculated means from both replicates for the fluorescence after each time point

Conclusively, these data suggest that the Interlab study was successful and that the provided protocol can be shared in the community for standardized fluorescence measurement with a plate reader.

Difference between revisions of "Team:Heidelberg/InterLab"

Latest revision as of 20:57, 1 November 2017

1. Interlab Study

1.1 Transformation of 8 test devices into competent DH5α cells

1.2 Measurement of LUDOX-HS40 OD600 Reference Point

1.3 Graphing a Fluorescein fluorescence standard curve

1.4 Measurement of OD₆₀₀ and Fluorescence of Transformed Cells

2. Discussion

Quote

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+{{Heidelberg/header}}
+{{Heidelberg/navbar}}
+{{Heidelberg/main|Interlab||https://static.igem.org/mediawiki/2017/a/ae/T--Heidelberg--2017_Background_Tiger.jpg|green|
+    {{Heidelberg/templateus/Contentsection|
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+            <h1>1. Interlab Study</h1>
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+            As a first step to contribute to this year's iGEM competition we decided to participate in iGEM's fourth International Interlaboratory Study along with many other teams from around the world. This study is organized by iGEM's measurement committee in an effort to establish standardized, reliable and repeatable measurement tools for the iGEM community and the synthetic biology community as a whole. This year's iGEM InterLab study is about establishing a standardized protocol for the measurement of GFP using a plate reader. To start things off we needed a plate reader that is qualified to measure GFP fluorescence. Namely, Tecan Infinite M200 Pro plate reader. Additionally, we needed competent <i>E.coli</i> DH5&#945;. These were prepared from glycerol stocks. Together with the material iGEM had provided we were ready for work. Throughout our experiments, we tested 8 plasmids (2 controls and 6 test devices) by measuring the OD<sub>600</sub> and the fluorescence of the cells carrying the constructs.
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+            The workflow can be separated into four segments. The first segment is the transformation of all plasmids into competent  DH5&#945; cells.
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+            <h2>1.1 Transformation of 8 test devices into competent DH5&#945; cells</h2>
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+            The transformation was successful for all the plasmids and resulted in a sufficient amount of colonies on all plates. Therefore, two colonies from each plate were picked and inoculated in LB medium containing chloramphenicol overnight for approximately 16-18 hours at 37°C and 220 rpm.
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+            <h2>1.2 Measurement of LUDOX-HS40 OD600 Reference Point</h2>
+            The second segment is the measurement of the LUDOX-HS40 solution to obtain a ratiometric conversion factor which allows us to transform the absorbance data into a standard OD<sub>600</sub> measurement. This is necessary because plate readers, in general, do not have a 1 cm path length which means that measurements are volume dependent. By doing so, we obtained following data.
+              {{Heidelberg/templateus/Tablebox|
+                    Table 1:|
+                    {{#tag:html|
+                        <table class="table table-bordered mdl-shadow--4dp" XSSCleaned="overflow-x: auto !important">
+                            <thead style="background-color: #005493 !important;">
+                              <tr>
+                                <th></th>
+                                <th>LUDOX-HS40</th>
+                                <th>H2O</th>
+                              </tr>
+                            </thead>
+                            <tbody>
+                              <tr>
+                                <td>Replicate<br>  1</td>
+                                <td>0,0543</td>
+                                <td>0,0424</td>
+                              </tr>
+                              <tr>
+                                <td>Replicate<br>  2</td>
+                                <td>0,0508</td>
+                                <td>0,0413</td>
+                              </tr>
+                              <tr>
+                                <td>Replicate<br>  3</td>
+                                <td>0,0515</td>
+                                <td>0,0424</td>
+                              </tr>
+                              <tr>
+                                <td>Replicate<br>  4</td>
+                                <td>0,051</td>
+                                <td>0,042</td>
+                              </tr>
+                              <tr>
+                                <td>Arith.<br>  Mean</td>
+                                <td>0,0519</td>
+                                <td>0,042</td>
+                              </tr>
+                              <tr>
+                                <td>Corrected<br>  Abs600</td>
+                                <td>0,009875</td>
+                                <td></td>
+                              </tr>
+                              <tr>
+                                <td>Reference<br>  OD600</td>
+                                <td>0,0425</td>
+                                <td></td>
+                              </tr>
+                              <tr>
+                                <td>OD600/Abs600</td>
+                                <td>4,303797</td>
+                                <td></td>
+                              </tr>
+                             </tbody>
+                            </table>
+                            }}|{{#tag:html|With these replicates measured, we obtained a ratiometric conversion factor of 4.30 for our specific plate reader when measured 100 &#956;L of the provided LUDOX-HS40 to water.}}}}
+        }}
+    }}
+    {{Heidelberg/templateus/Contentsection|
+        {{#tag:html|
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+            <h2>1.3 Graphing a Fluorescein fluorescence standard curve</h2>
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+            In the third segment, we were instructed to measure a standard curve for fluorescence of Fluorescein which can be used to correct our cell-based readings to an equivalent Fluorescein concentration. Following the instructions, we could obtain following results for the serial dilution of Fluorescein in phosphate buffered saline.
-		<div id="content" class="mw-body" role="main">
-		    <a id="top"></a>
+   {{Heidelberg/templateus/Tablebox|
+                    Table 2:|
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+                        <table class="table table-bordered mdl-shadow--4dp" XSSCleaned="overflow-x: auto !important">
+                            <thead style="background-color: #005493 !important;">
+                                <th class="tg-031e">uM Fluorescein</th>
+                                <th class="tg-031e">50,00</th>
+                                <th class="tg-031e">25</th>
+                                <th>12,5</th>
+                                <th>6,25</th>
+                                <th>3,125</th>
+                                <th>1,5625</th>
+                                <th>0,7813</th>
+                                <th>0,3906</th>
+                                <th>0,1953</th>
+                                <th>0,0977</th>
+                                <th>0,0488</th>
+                                <th>0</th>
+                            </thead>
+                            <tbody>
+                            <tr>
+                                <td class="tg-031e">Replicate 1</td>
+                                <td class="tg-031e">43791</td>
+                                <td class="tg-031e">28969</td>
+                                <td>17384</td>
+                                <td>9562</td>
+                                <td>5001</td>
+                                <td>2591</td>
+                                <td>1355</td>
+                                <td>676</td>
+                                <td>344</td>
+                                <td>173</td>
+                                <td>87</td>
+                                <td>3</td>
+                              </tr>
+                              <tr>
+                                <td class="tg-031e">Replicate 2</td>
+                                <td class="tg-031e">44517</td>
+                                <td class="tg-031e">29931</td>
+                                <td>17243</td>
+                                <td>9266</td>
+                                <td>5010</td>
+                                <td>2575</td>
+                                <td>1325</td>
+                                <td>668</td>
+                                <td>324</td>
+                                <td>170</td>
+                                <td>84</td>
+                                <td>2</td>
+                              </tr>
+                              <tr>
+                                <td>Replicate 3</td>
+                                <td>42992</td>
+                                <td>28755</td>
+                                <td>17684</td>
+                                <td>10262</td>
+                                <td>5451</td>
+                                <td>2851</td>
+                                <td>1439</td>
+                                <td>733</td>
+                                <td>369</td>
+                                <td>184</td>
+                                <td>91</td>
+                                <td>2</td>
+                              </tr>
+                              <tr>
+                                <td>Replicate 4</td>
+                                <td>42990</td>
+                                <td>28734</td>
+                                <td>17280</td>
+                                <td>9617</td>
+                                <td>5261</td>
+                                <td>2667</td>
+                                <td>1381</td>
+                                <td>696</td>
+                                <td>337</td>
+                                <td>178</td>
+                                <td>90</td>
+                                <td>5</td>
+                              </tr>
+                              <tr>
+                                <td>Arith. Mean</td>
+                                <td>43573</td>
+                                <td>29097</td>
+                                <td>17398</td>
+                                <td>9676,8</td>
+                                <td>5180,8</td>
+                                <td>2671</td>
+                                <td>1375</td>
+                                <td>693,25</td>
+                                <td>343,5</td>
+                                <td>176,25</td>
+                                <td>88</td>
+                                <td>3</td>
+                              </tr>
+                              <tr>
+                                <td>Arith. Std.Dev.</td>
+                                <td>733,96</td>
+                                <td>565,88</td>
+                                <td>199,95</td>
+                                <td>419,51</td>
+                                <td>216,75</td>
+                                <td>126,53</td>
+                                <td>48,415</td>
+                                <td>28,999</td>
+                                <td>18,912</td>
+                                <td>6,1305</td>
+                                <td>3,1623</td>
+                                <td>1,4142</td>
+                              </tr>
+                              </tbody>
+                            </table>
+                            }}|}}
-            <div id="top_title">
+{{Heidelberg/templateus/Tablebox|
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+                    Table 3:|
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+                    {{#tag:html|
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-                    </a>
+                            <thead style="background-color: #005493 !important;">
-                </div>
+                                  <tr>
+                                    <th class="tg-031e">uM<br>  Fluorescein/a.u.</th>
+                                    <th class="tg-031e">50,00</th>
+                                    <th class="tg-031e">25</th>
+                                    <th>12,5</th>
+                                    <th>6,25</th>
+                                    <th>3,125</th>
+                                    <th>1,5625</th>
+                                    <th>0,7813</th>
+                                    <th>0,3906</th>
+                                    <th>0,1953</th>
+                                    <th>0,0977</th>
+                                    <th>0,0488</th>
+                                          </tr>
+                                  </thead>
+                                <tbody>
+                                  <tr>
+                                    <td class="tg-031e">Mean uM Fluorescein/a.u.</td>
+                                    <td class="tg-031e">0,0011</td>
+                                    <td class="tg-031e">0,0009</td>
+                                    <td>0,0007</td>
+                                    <td>0,0006</td>
+                                    <td>0,0006</td>
+                                    <td>0,0006</td>
+                                    <td>0,0006</td>
+                                    <td>0,0006</td>
+                                    <td>0,0006</td>
+                                    <td>0,0006</td>
+                                    <td>0,0006</td>
+                                  </tr>
+                                  <tr>
+                                    <td class="tg-031e">Mean of<br>  med-high levels:</td>
+                                    <td class="tg-031e">0,0007</td>
+                                    <td class="tg-031e"></td>
+                                    <td></td>
+                                    <td></td>
+                                    <td></td>
+                                    <td></td>
+                                    <td></td>
+                                    <td></td>
+                                    <td></td>
+                                    <td></td>
+                                    <td></td>
+                                  </tr>
+                                </tbody>
+                                </table>
+                      }}|}}
- 		        <h1 id="firstHeading" class="firstHeading">
-		            <span dir="auto">Team:Heidelberg/internal tools</span>
+            {{Heidelberg/templateus/Imagebox|https://static.igem.org/mediawiki/2017/5/57/T--Heidelberg--FluoresceinStandardCurve.png|Figure 1.1:| Fluorescence is plotted against the Fluorescein concentration|pos = left}}
-		        </h1>
-            </div>
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+             {{Heidelberg/templateus/Imagebox|https://static.igem.org/mediawiki/2017/f/fa/T--Heidelberg--FluoresceinStandardCurveLogScale.png|Figure 1.2:| Fluorescence is plotted against the Fluorescein concentration on a logarithmic scale|pos = right}}
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+            These data give us an approximate linear regression line when plotted on a logarithmic scale and show an expected exponential course when plotted in a linear form.
+        }}
+    }}
+    {{Heidelberg/templateus/Contentsection|
+        {{#tag:html|
-     <div id="top-section">
+            <h2>1.4 Measurement of OD<sub>600</sub> and Fluorescence of Transformed Cells</h2>
-	<script type="text/javascript"> if (window.isMSIE55) fixalpha(); </script>
+            In the last of the four segments, the OD<sub>600</sub> and the fluorescence of the transformed cells were measured according to the protocol after 0, 2, 4 and 6 hours. Measurements of all these time points gave us the following data and calculations which were conducted with the values we obtained from the standard curves and the reference point.
+          {{Heidelberg/templateus/Tablebox|
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+                                  <tr>
+    <th class="tg-s6z2" colspan="10">Fluorescence Raw Readings:</th>
+    <th class="tg-baqh" colspan="10">Abs600Raw Readings:</th>
+  </tr>
+                            </thead>
+                            <tbody>
+  <tr>
+    <td class="tg-031e">Hour 0:</td>
+    <td class="tg-031e">Neg. Control</td>
+    <td class="tg-031e">Pos. Control</td>
+    <td>Device 1</td>
+    <td>Device 2</td>
+    <td>Device 3</td>
+    <td>Device 4</td>
+    <td>Device 5</td>
+    <td>Device 6</td>
+    <td>LB + Chlor (blank)</td>
+    <td>Hour 0:</td>
+    <td>Neg. Control</td>
+    <td>Pos. Control</td>
+    <td>Device 1</td>
+    <td>Device 2</td>
+    <td>Device 3</td>
+    <td>Device 4</td>
+    <td>Device 5</td>
+    <td>Device 6</td>
+    <td>LB + Chlor (blank)</td>
+  </tr>
+  <tr>
+    <td class="tg-031e">Colony 1, Replicate 1</td>
+    <td class="tg-031e">228</td>
+    <td class="tg-031e">262</td>
+    <td>294</td>
+    <td>371</td>
+    <td>227</td>
+    <td>275</td>
+    <td>249</td>
+    <td>230</td>
+    <td>221</td>
+    <td>Colony 1, Replicate 1</td>
+    <td>0,0473</td>
+    <td>0,0467</td>
+    <td>0,0478</td>
+    <td>0,0493</td>
+    <td>0,0483</td>
+    <td>0,0486</td>
+    <td>0,0474</td>
+    <td>0,0456</td>
+    <td>0,0435</td>
+  </tr>
+  <tr>
+    <td>Colony 1, Replicate 2</td>
+    <td>222</td>
+    <td>298</td>
+    <td>270</td>
+    <td>302</td>
+    <td>214</td>
+    <td>271</td>
+    <td>237</td>
+    <td>230</td>
+    <td>242</td>
+    <td>Colony 1, Replicate 2</td>
+    <td>0,0459</td>
+    <td>0,0466</td>
+    <td>0,0464</td>
+    <td>0,0468</td>
+    <td>0,0471</td>
+    <td>0,0474</td>
+    <td>0,0459</td>
+    <td>0,0442</td>
+    <td>0,043</td>
+  </tr>
+  <tr>
+    <td>Colony 1, Replicate 3</td>
+    <td>196</td>
+    <td>265</td>
+    <td>269</td>
+    <td>285</td>
+    <td>227</td>
+    <td>268</td>
+    <td>231</td>
+    <td>219</td>
+    <td>205</td>
+    <td>Colony 1, Replicate 3</td>
+    <td>0,05</td>
+    <td>0,0499</td>
+    <td>0,0502</td>
+    <td>0,0503</td>
+    <td>0,0518</td>
+    <td>0,0513</td>
+    <td>0,0502</td>
+    <td>0,0479</td>
+    <td>0,0473</td>
+  </tr>
+  <tr>
+    <td>Colony 1, Replicate 4</td>
+    <td>227</td>
+    <td>302</td>
+    <td>285</td>
+    <td>312</td>
+    <td>225</td>
+    <td>272</td>
+    <td>244</td>
+    <td>232</td>
+    <td>235</td>
+    <td>Colony 1, Replicate 4</td>
+    <td>0,061</td>
+    <td>0,0667</td>
+    <td>0,0691</td>
+    <td>0,0714</td>
+    <td>0,0725</td>
+    <td>0,0709</td>
+    <td>0,0596</td>
+    <td>0,0624</td>
+    <td>0,0412</td>
+  </tr>
+  <tr>
+    <td>Colony 2, Replicate 1</td>
+    <td>226</td>
+    <td>258</td>
+    <td>297</td>
+    <td>287</td>
+    <td>230</td>
+    <td>284</td>
+    <td>255</td>
+    <td>221</td>
+    <td>221</td>
+    <td>Colony 2, Replicate 1</td>
+    <td>0,0473</td>
+    <td>0,0471</td>
+    <td>0,0504</td>
+    <td>0,0484</td>
+    <td>0,0482</td>
+    <td>0,0482</td>
+    <td>0,0496</td>
+    <td>0,0482</td>
+    <td>0,0474</td>
+  </tr>
+  <tr>
+    <td>Colony 2, Replicate 2</td>
+    <td>217</td>
+    <td>277</td>
+    <td>301</td>
+    <td>311</td>
+    <td>221</td>
+    <td>283</td>
+    <td>244</td>
+    <td>244</td>
+    <td>231</td>
+    <td>Colony 2, Replicate 2</td>
+    <td>0,0469</td>
+    <td>0,0472</td>
+    <td>0,0492</td>
+    <td>0,0485</td>
+    <td>0,0473</td>
+    <td>0,047</td>
+    <td>0,0479</td>
+    <td>0,0474</td>
+    <td>0,0477</td>
+  </tr>
+  <tr>
+    <td>Colony 2, Replicate 3</td>
+    <td>213</td>
+    <td>261</td>
+    <td>287</td>
+    <td>285</td>
+    <td>222</td>
+    <td>276</td>
+    <td>236</td>
+    <td>225</td>
+    <td>234</td>
+    <td>Colony 2, Replicate 3</td>
+    <td>0,0512</td>
+    <td>0,0499</td>
+    <td>0,0563</td>
+    <td>0,0527</td>
+    <td>0,052</td>
+    <td>0,0523</td>
+    <td>0,0539</td>
+    <td>0,0533</td>
+    <td>0,0521</td>
+  </tr>
+  <tr>
+    <td>Colony 2, Replicate 4</td>
+    <td>223</td>
+    <td>267</td>
+    <td>294</td>
+    <td>303</td>
+    <td>224</td>
+    <td>293</td>
+    <td>249</td>
+    <td>238</td>
+    <td>243</td>
+    <td>Colony 2, Replicate 4</td>
+    <td>0,0604</td>
+    <td>0,0696</td>
+    <td>0,0686</td>
+    <td>0,0617</td>
+    <td>0,0719</td>
+    <td>0,0714</td>
+    <td>0,0608</td>
+    <td>0,0611</td>
+    <td>0,0646</td>
+  </tr>
+  <tr>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+  </tr>
+  <tr>
+    <td>Hour 2:</td>
+    <td>Neg. Control</td>
+    <td>Pos. Control</td>
+    <td>Device 1</td>
+    <td>Device 2</td>
+    <td>Device 3</td>
+    <td>Device 4</td>
+    <td>Device 5</td>
+    <td>Device 6</td>
+    <td>LB + Chlor (blank)</td>
+    <td>Hour 2:</td>
+    <td>Neg. Control</td>
+    <td>Pos. Control</td>
+    <td>Device 1</td>
+    <td>Device 2</td>
+    <td>Device 3</td>
+    <td>Device 4</td>
+    <td>Device 5</td>
+    <td>Device 6</td>
+    <td>LB + Chlor (blank)</td>
+  </tr>
+  <tr>
+    <td>Colony 1, Replicate 1</td>
+    <td>234</td>
+    <td>841</td>
+    <td>916</td>
+    <td>973</td>
+    <td>241</td>
+    <td>640</td>
+    <td>740</td>
+    <td>242</td>
+    <td>222</td>
+    <td>Colony 1, Replicate 1</td>
+    <td>0,0652</td>
+    <td>0,0568</td>
+    <td>0,0599</td>
+    <td>0,0613</td>
+    <td>0,068</td>
+    <td>0,0521</td>
+    <td>0,0742</td>
+    <td>0,075</td>
+    <td>0,0465</td>
+  </tr>
+  <tr>
+    <td>Colony 1, Replicate 2</td>
+    <td>229</td>
+    <td>850</td>
+    <td>864</td>
+    <td>923</td>
+    <td>234</td>
+    <td>608</td>
+    <td>641</td>
+    <td>239</td>
+    <td>239</td>
+    <td>Colony 1, Replicate 2</td>
+    <td>0,06</td>
+    <td>0,0539</td>
+    <td>0,0569</td>
+    <td>0,057</td>
+    <td>0,0632</td>
+    <td>0,0496</td>
+    <td>0,0671</td>
+    <td>0,0713</td>
+    <td>0,0455</td>
+  </tr>
+  <tr>
+    <td>Colony 1, Replicate 3</td>
+    <td>230</td>
+    <td>741</td>
+    <td>810</td>
+    <td>897</td>
+    <td>236</td>
+    <td>551</td>
+    <td>545</td>
+    <td>254</td>
+    <td>236</td>
+    <td>Colony 1, Replicate 3</td>
+    <td>0,0671</td>
+    <td>0,057</td>
+    <td>0,0626</td>
+    <td>0,0637</td>
+    <td>0,0671</td>
+    <td>0,0547</td>
+    <td>0,0698</td>
+    <td>0,0754</td>
+    <td>0,0515</td>
+  </tr>
+  <tr>
+    <td>Colony 1, Replicate 4</td>
+    <td>227</td>
+    <td>758</td>
+    <td>942</td>
+    <td>893</td>
+    <td>240</td>
+    <td>564</td>
+    <td>613</td>
+    <td>241</td>
+    <td>240</td>
+    <td>Colony 1, Replicate 4</td>
+    <td>0,081</td>
+    <td>0,0695</td>
+    <td>0,082</td>
+    <td>0,0799</td>
+    <td>0,0833</td>
+    <td>0,0674</td>
+    <td>0,0836</td>
+    <td>0,085</td>
+    <td>0,0409</td>
+  </tr>
+  <tr>
+    <td>Colony 2, Replicate 1</td>
+    <td>233</td>
+    <td>913</td>
+    <td>1016</td>
+    <td>1045</td>
+    <td>246</td>
+    <td>521</td>
+    <td>661</td>
+    <td>244</td>
+    <td>215</td>
+    <td>Colony 2, Replicate 1</td>
+    <td>0,0667</td>
+    <td>0,0602</td>
+    <td>0,062</td>
+    <td>0,065</td>
+    <td>0,0704</td>
+    <td>0,0499</td>
+    <td>0,0729</td>
+    <td>0,0774</td>
+    <td>0,0491</td>
+  </tr>
+  <tr>
+    <td>Colony 2, Replicate 2</td>
+    <td>224</td>
+    <td>784</td>
+    <td>878</td>
+    <td>893</td>
+    <td>243</td>
+    <td>702</td>
+    <td>642</td>
+    <td>233</td>
+    <td>254</td>
+    <td>Colony 2, Replicate 2</td>
+    <td>0,0608</td>
+    <td>0,0558</td>
+    <td>0,0568</td>
+    <td>0,0592</td>
+    <td>0,0654</td>
+    <td>0,052</td>
+    <td>0,068</td>
+    <td>0,0721</td>
+    <td>0,0452</td>
+  </tr>
+  <tr>
+    <td>Colony 2, Replicate 3</td>
+    <td>217</td>
+    <td>712</td>
+    <td>765</td>
+    <td>877</td>
+    <td>228</td>
+    <td>597</td>
+    <td>571</td>
+    <td>219</td>
+    <td>232</td>
+    <td>Colony 2, Replicate 3</td>
+    <td>0,0642</td>
+    <td>0,0614</td>
+    <td>0,0624</td>
+    <td>0,0669</td>
+    <td>0,0719</td>
+    <td>0,0584</td>
+    <td>0,0732</td>
+    <td>0,0676</td>
+    <td>0,053</td>
+  </tr>
+  <tr>
+    <td>Colony 2, Replicate 4</td>
+    <td>227</td>
+    <td>706</td>
+    <td>748</td>
+    <td>904</td>
+    <td>237</td>
+    <td>598</td>
+    <td>576</td>
+    <td>252</td>
+    <td>247</td>
+    <td>Colony 2, Replicate 4</td>
+    <td>0,0684</td>
+    <td>0,057</td>
+    <td>0,071</td>
+    <td>0,0803</td>
+    <td>0,0959</td>
+    <td>0,0653</td>
+    <td>0,0843</td>
+    <td>0,0848</td>
+    <td>0,0732</td>
+  </tr>
+  <tr>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+  </tr>
+  <tr>
+    <td>Hour 4:</td>
+    <td>Neg. Control</td>
+    <td>Pos. Control</td>
+    <td>Device 1</td>
+    <td>Device 2</td>
+    <td>Device 3</td>
+    <td>Device 4</td>
+    <td>Device 5</td>
+    <td>Device 6</td>
+    <td>LB + Chlor (blank)</td>
+    <td>Hour 4:</td>
+    <td>Neg. Control</td>
+    <td>Pos. Control</td>
+    <td>Device 1</td>
+    <td>Device 2</td>
+    <td>Device 3</td>
+    <td>Device 4</td>
+    <td>Device 5</td>
+    <td>Device 6</td>
+    <td>LB + Chlor (blank)</td>
+  </tr>
+  <tr>
+    <td>Colony 1, Replicate 1</td>
+    <td>265</td>
+    <td>3725</td>
+    <td>4330</td>
+    <td>4534</td>
+    <td>327</td>
+    <td>1619</td>
+    <td>1493</td>
+    <td>293</td>
+    <td>224</td>
+    <td>Colony 1, Replicate 1</td>
+    <td>0,1765</td>
+    <td>0,1449</td>
+    <td>0,1431</td>
+    <td>0,1661</td>
+    <td>0,1677</td>
+    <td>0,0692</td>
+    <td>0,1766</td>
+    <td>0,193</td>
+    <td>0,047</td>
+  </tr>
+  <tr>
+    <td>Colony 1, Replicate 2</td>
+    <td>269</td>
+    <td>3980</td>
+    <td>3936</td>
+    <td>4087</td>
+    <td>334</td>
+    <td>1489</td>
+    <td>1551</td>
+    <td>275</td>
+    <td>229</td>
+    <td>Colony 1, Replicate 2</td>
+    <td>0,1804</td>
+    <td>0,1515</td>
+    <td>0,1365</td>
+    <td>0,1585</td>
+    <td>0,1861</td>
+    <td>0,07</td>
+    <td>0,19</td>
+    <td>0,167</td>
+    <td>0,0469</td>
+  </tr>
+  <tr>
+    <td>Colony 1, Replicate 3</td>
+    <td>245</td>
+    <td>2579</td>
+    <td>3110</td>
+    <td>3080</td>
+    <td>300</td>
+    <td>1280</td>
+    <td>1257</td>
+    <td>276</td>
+    <td>238</td>
+    <td>Colony 1, Replicate 3</td>
+    <td>0,1321</td>
+    <td>0,1194</td>
+    <td>0,1199</td>
+    <td>0,132</td>
+    <td>0,1482</td>
+    <td>0,0666</td>
+    <td>0,1573</td>
+    <td>0,1578</td>
+    <td>0,0516</td>
+  </tr>
+  <tr>
+    <td>Colony 1, Replicate 4</td>
+    <td>247</td>
+    <td>2934</td>
+    <td>3253</td>
+    <td>3214</td>
+    <td>313</td>
+    <td>1543</td>
+    <td>1358</td>
+    <td>304</td>
+    <td>234</td>
+    <td>Colony 1, Replicate 4</td>
+    <td>0,1354</td>
+    <td>0,1348</td>
+    <td>0,1296</td>
+    <td>0,1441</td>
+    <td>0,1762</td>
+    <td>0,0885</td>
+    <td>0,164</td>
+    <td>0,189</td>
+    <td>0,0456</td>
+  </tr>
+  <tr>
+    <td>Colony 2, Replicate 1</td>
+    <td>269</td>
+    <td>3980</td>
+    <td>3936</td>
+    <td>4087</td>
+    <td>334</td>
+    <td>1489</td>
+    <td>1551</td>
+    <td>275</td>
+    <td>229</td>
+    <td>Colony 2, Replicate 1</td>
+    <td>0,3007</td>
+    <td>0,2746</td>
+    <td>0,2586</td>
+    <td>0,308</td>
+    <td>0,3246</td>
+    <td>0,339</td>
+    <td>0,3438</td>
+    <td>0,3446</td>
+    <td>0,0473</td>
+  </tr>
+  <tr>
+    <td>Colony 2, Replicate 2</td>
+    <td>253</td>
+    <td>2825</td>
+    <td>3064</td>
+    <td>3547</td>
+    <td>321</td>
+    <td>1619</td>
+    <td>1294</td>
+    <td>308</td>
+    <td>237</td>
+    <td>Colony 2, Replicate 2</td>
+    <td>0,1467</td>
+    <td>0,1192</td>
+    <td>0,1135</td>
+    <td>0,1373</td>
+    <td>0,1643</td>
+    <td>0,0698</td>
+    <td>0,151</td>
+    <td>0,1849</td>
+    <td>0,0453</td>
+  </tr>
+  <tr>
+    <td>Colony 2, Replicate 3</td>
+    <td>249</td>
+    <td>3397</td>
+    <td>3055</td>
+    <td>3125</td>
+    <td>309</td>
+    <td>1516</td>
+    <td>1238</td>
+    <td>276</td>
+    <td>239</td>
+    <td>Colony 2, Replicate 3</td>
+    <td>0,1374</td>
+    <td>0,1356</td>
+    <td>0,1199</td>
+    <td>0,1344</td>
+    <td>0,1453</td>
+    <td>0,0763</td>
+    <td>0,1532</td>
+    <td>0,1626</td>
+    <td>0,0536</td>
+  </tr>
+  <tr>
+    <td>Colony 2, Replicate 4</td>
+    <td>261</td>
+    <td>2585</td>
+    <td>3302</td>
+    <td>3124</td>
+    <td>322</td>
+    <td>1589</td>
+    <td>1285</td>
+    <td>292</td>
+    <td>246</td>
+    <td>Colony 2, Replicate 4</td>
+    <td>0,1423</td>
+    <td>0,1255</td>
+    <td>0,138</td>
+    <td>0,1527</td>
+    <td>0,1686</td>
+    <td>0,0765</td>
+    <td>0,1522</td>
+    <td>0,1622</td>
+    <td>0,064</td>
+  </tr>
+  <tr>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+  </tr>
+  <tr>
+    <td>Hour 6:</td>
+    <td>Neg. Control</td>
+    <td>Pos. Control</td>
+    <td>Device 1</td>
+    <td>Device 2</td>
+    <td>Device 3</td>
+    <td>Device 4</td>
+    <td>Device 5</td>
+    <td>Device 6</td>
+    <td>LB + Chlor (blank)</td>
+    <td>Hour 6:</td>
+    <td>Neg. Control</td>
+    <td>Pos. Control</td>
+    <td>Device 1</td>
+    <td>Device 2</td>
+    <td>Device 3</td>
+    <td>Device 4</td>
+    <td>Device 5</td>
+    <td>Device 6</td>
+    <td>LB + Chlor (blank)</td>
+  </tr>
+  <tr>
+    <td>Colony 1, Replicate 1</td>
+    <td>289</td>
+    <td>7046</td>
+    <td>9753</td>
+    <td>10223</td>
+    <td>429</td>
+    <td>4924</td>
+    <td>5031</td>
+    <td>328</td>
+    <td>223</td>
+    <td>Colony 1, Replicate 1</td>
+    <td>0,2911</td>
+    <td>0,2572</td>
+    <td>0,2697</td>
+    <td>0,2843</td>
+    <td>0,311</td>
+    <td>0,1557</td>
+    <td>0,1581</td>
+    <td>0,3271</td>
+    <td>0,0473</td>
+  </tr>
+  <tr>
+    <td>Colony 1, Replicate 2</td>
+    <td>290</td>
+    <td>7161</td>
+    <td>9621</td>
+    <td>9685</td>
+    <td>429</td>
+    <td>4659</td>
+    <td>4916</td>
+    <td>322</td>
+    <td>233</td>
+    <td>Colony 1, Replicate 2</td>
+    <td>0,2735</td>
+    <td>0,2467</td>
+    <td>0,2558</td>
+    <td>0,2596</td>
+    <td>0,2984</td>
+    <td>0,1439</td>
+    <td>0,1471</td>
+    <td>0,3082</td>
+    <td>0,0492</td>
+  </tr>
+  <tr>
+    <td>Colony 1, Replicate 3</td>
+    <td>284</td>
+    <td>6900</td>
+    <td>8983</td>
+    <td>9620</td>
+    <td>420</td>
+    <td>4563</td>
+    <td>4830</td>
+    <td>335</td>
+    <td>236</td>
+    <td>Colony 1, Replicate 3</td>
+    <td>0,2694</td>
+    <td>0,2542</td>
+    <td>0,2557</td>
+    <td>0,2671</td>
+    <td>0,2856</td>
+    <td>0,1464</td>
+    <td>0,1531</td>
+    <td>0,3093</td>
+    <td>0,052</td>
+  </tr>
+  <tr>
+    <td>Colony 1, Replicate 4</td>
+    <td>320</td>
+    <td>7664</td>
+    <td>10384</td>
+    <td>10759</td>
+    <td>441</td>
+    <td>5092</td>
+    <td>5472</td>
+    <td>341</td>
+    <td>239</td>
+    <td>Colony 1, Replicate 4</td>
+    <td>0,3314</td>
+    <td>0,2762</td>
+    <td>0,2711</td>
+    <td>0,2787</td>
+    <td>0,2999</td>
+    <td>0,1738</td>
+    <td>0,1763</td>
+    <td>0,2938</td>
+    <td>0,0401</td>
+  </tr>
+  <tr>
+    <td>Colony 2, Replicate 1</td>
+    <td>285</td>
+    <td>7246</td>
+    <td>9059</td>
+    <td>10726</td>
+    <td>445</td>
+    <td>2372</td>
+    <td>2368</td>
+    <td>332</td>
+    <td>229</td>
+    <td>Colony 2, Replicate 1</td>
+    <td>0,3007</td>
+    <td>0,2746</td>
+    <td>0,2586</td>
+    <td>0,308</td>
+    <td>0,3246</td>
+    <td>0,339</td>
+    <td>0,3438</td>
+    <td>0,3446</td>
+    <td>0,0473</td>
+  </tr>
+  <tr>
+    <td>Colony 2, Replicate 2</td>
+    <td>286</td>
+    <td>7418</td>
+    <td>10409</td>
+    <td>10079</td>
+    <td>442</td>
+    <td>2357</td>
+    <td>2321</td>
+    <td>329</td>
+    <td>232</td>
+    <td>Colony 2, Replicate 2</td>
+    <td>0,2853</td>
+    <td>0,2574</td>
+    <td>0,2783</td>
+    <td>0,2668</td>
+    <td>0,2989</td>
+    <td>0,3188</td>
+    <td>0,3134</td>
+    <td>0,3161</td>
+    <td>0,0513</td>
+  </tr>
+  <tr>
+    <td>Colony 2, Replicate 3</td>
+    <td>285</td>
+    <td>6939</td>
+    <td>9332</td>
+    <td>10013</td>
+    <td>439</td>
+    <td>2297</td>
+    <td>2299</td>
+    <td>336</td>
+    <td>232</td>
+    <td>Colony 2, Replicate 3</td>
+    <td>0,282</td>
+    <td>0,2546</td>
+    <td>0,2629</td>
+    <td>0,2691</td>
+    <td>0,3006</td>
+    <td>0,3064</td>
+    <td>0,3063</td>
+    <td>0,3094</td>
+    <td>0,0538</td>
+  </tr>
+  <tr>
+    <td>Colony 2, Replicate 4</td>
+    <td>294</td>
+    <td>7882</td>
+    <td>9938</td>
+    <td>10980</td>
+    <td>446</td>
+    <td>2523</td>
+    <td>2513</td>
+    <td>330</td>
+    <td>253</td>
+    <td>Colony 2, Replicate 4</td>
+    <td>0,2851</td>
+    <td>0,2633</td>
+    <td>0,2614</td>
+    <td>0,2668</td>
+    <td>0,2911</td>
+    <td>0,3239</td>
+    <td>0,336</td>
+    <td>0,3191</td>
+    <td>0,0469</td>
+  </tr>
+</tbody>
+</table>
+}}|}}
+{{Heidelberg/templateus/Tablebox|
+                    Table 5:|
+                    {{#tag:html|
+                        <table class="table table-bordered mdl-shadow--4dp" XSSCleaned="overflow-x: auto !important">
+                            <thead style="background-color: #005493 !important;">
+  <tr>
+    <th class="tg-031e">Unit<br>  Scaling Factors:</th>
+    <th></th>
+  </tr>
+</thead>
+<tbody>
+  <tr>
+    <td class="tg-031e">OD600/Abs600</td>
+    <td class="tg-031e">4,30</td>
+  </tr>
+  <tr>
+    <td class="tg-031e">uM Fluorescein/a.u.</td>
+    <td class="tg-031e">6,82E-04</td>
+  </tr>
+</tbody>
+</table>
+}}|}}
+{{Heidelberg/templateus/Tablebox|
+                    Table 6:|
+                    {{#tag:html|
+                        <table class="table table-bordered mdl-shadow--4dp" XSSCleaned="overflow-x: auto !important">
+                            <thead style="background-color: #005493 !important;">
+                                <tr>
+    <th class="tg-031e">Experimental<br>  Values:</th>
+    <th class="tg-s6z2" colspan="4">Raw Abs600</th>
+    <th class="tg-baqh" colspan="4">Raw<br>  Fluorescence</th>
+    <th></th>
+    <th></th>
+    <th></th>
+    <th></th>
+    <th></th>
+    <th></th>
+    <th></th>
+    <th></th>
+    <th></th>
+    <th></th>
+    <th></th>
+    <th></th>
+    <th></th>
+    <th></th>
+    <th></th>
+    <th></th>
+  </tr>
+                            </thead>
+                            <tbody>
+  <tr>
+    <td class="tg-031e">Sample set:</td>
+    <td class="tg-031e">Replicate 1</td>
+    <td>Replicate 2</td>
+    <td>Replicate 3</td>
+    <td>Replicate 4</td>
+    <td>Replicate 1</td>
+    <td>Replicate 2</td>
+    <td>Replicate 3</td>
+    <td>Replicate 4</td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+  </tr>
+  <tr>
+    <td class="tg-031e">Blank media</td>
+    <td class="tg-031e">0,0435</td>
+    <td>0,043</td>
+    <td>0,0473</td>
+    <td>0,0412</td>
+    <td>221</td>
+    <td>242</td>
+    <td>205</td>
+    <td>235</td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+  </tr>
+  <tr>
+    <td>Blank mean:</td>
+    <td>0,04375</td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td>225,75</td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td class="tg-baqh" colspan="4">OD - Background</td>
+    <td colspan="4">Fluorescence - Background</td>
+    <td class="tg-baqh" colspan="4">uM Fluorescein / OD600</td>
+    <td class="tg-baqh" colspan="4">Summary Statistics</td>
+  </tr>
+  <tr>
+    <td>Hour 0:</td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td>Replicate 1</td>
+    <td>Replicate 2</td>
+    <td>Replicate 3</td>
+    <td>Replicate 4</td>
+    <td>Replicate 1</td>
+    <td>Replicate 2</td>
+    <td>Replicate 3</td>
+    <td>Replicate 4</td>
+    <td>Replicate 1</td>
+    <td>Replicate 2</td>
+    <td>Replicate 3</td>
+    <td>Replicate 4</td>
+    <td>Arith. Mean</td>
+    <td>Arith. Std.Dev.</td>
+    <td>Geo. Mean</td>
+    <td>Geo. Std. Dev.</td>
+  </tr>
+  <tr>
+    <td>Negative Control (Colony 1)</td>
+    <td>0,0473</td>
+    <td>0,0459</td>
+    <td>0,05</td>
+    <td>0,061</td>
+    <td>228</td>
+    <td>222</td>
+    <td>196</td>
+    <td>227</td>
+    <td>0,00355</td>
+    <td>0,00215</td>
+    <td>0,00625</td>
+    <td>0,01725</td>
+    <td>2,25</td>
+    <td>-3,75</td>
+    <td>-29,75</td>
+    <td>1,25</td>
+    <td>0,10</td>
+    <td>-0,28</td>
+    <td>-0,75</td>
+    <td>0,01</td>
+    <td>-0,23</td>
+    <td>0,39</td>
+    <td></td>
+    <td></td>
+  </tr>
+  <tr>
+    <td>Negative Control (Colony 2)</td>
+    <td>0,0473</td>
+    <td>0,0469</td>
+    <td>0,0512</td>
+    <td>0,0604</td>
+    <td>226</td>
+    <td>217</td>
+    <td>213</td>
+    <td>223</td>
+    <td>0,00355</td>
+    <td>0,00315</td>
+    <td>0,00745</td>
+    <td>0,01665</td>
+    <td>0,25</td>
+    <td>-8,75</td>
+    <td>-12,75</td>
+    <td>-2,75</td>
+    <td>0,01</td>
+    <td>-0,44</td>
+    <td>-0,27</td>
+    <td>-0,03</td>
+    <td>-0,18</td>
+    <td>0,21</td>
+    <td></td>
+    <td></td>
+  </tr>
+  <tr>
+    <td>Positive Control (Colony 1)</td>
+    <td>0,0467</td>
+    <td>0,0466</td>
+    <td>0,0499</td>
+    <td>0,0667</td>
+    <td>262</td>
+    <td>298</td>
+    <td>265</td>
+    <td>302</td>
+    <td>0,00295</td>
+    <td>0,00285</td>
+    <td>0,00615</td>
+    <td>0,02295</td>
+    <td>36,25</td>
+    <td>72,25</td>
+    <td>39,25</td>
+    <td>76,25</td>
+    <td>1,95</td>
+    <td>4,02</td>
+    <td>1,01</td>
+    <td>0,53</td>
+    <td>1,88</td>
+    <td>1,55</td>
+    <td>1,43</td>
+    <td>2,391</td>
+  </tr>
+  <tr>
+    <td>Positive Control (Colony 2)</td>
+    <td>0,0471</td>
+    <td>0,0472</td>
+    <td>0,0499</td>
+    <td>0,0696</td>
+    <td>258</td>
+    <td>277</td>
+    <td>261</td>
+    <td>267</td>
+    <td>0,00335</td>
+    <td>0,00345</td>
+    <td>0,00615</td>
+    <td>0,02585</td>
+    <td>32,25</td>
+    <td>51,25</td>
+    <td>35,25</td>
+    <td>41,25</td>
+    <td>1,53</td>
+    <td>2,36</td>
+    <td>0,91</td>
+    <td>0,25</td>
+    <td>1,26</td>
+    <td>0,90</td>
+    <td>0,95</td>
+    <td>2,628</td>
+  </tr>
+  <tr>
+    <td>Test Device 1: J23101.BCD2.E0040.B0015<br>  (Colony 1)</td>
+    <td>0,0478</td>
+    <td>0,0464</td>
+    <td>0,0502</td>
+    <td>0,0691</td>
+    <td>294</td>
+    <td>270</td>
+    <td>269</td>
+    <td>285</td>
+    <td>0,00405</td>
+    <td>0,00265</td>
+    <td>0,00645</td>
+    <td>0,02535</td>
+    <td>68,25</td>
+    <td>44,25</td>
+    <td>43,25</td>
+    <td>59,25</td>
+    <td>2,67</td>
+    <td>2,65</td>
+    <td>1,06</td>
+    <td>0,37</td>
+    <td>1,69</td>
+    <td>1,16</td>
+    <td>1,29</td>
+    <td>2,555</td>
+  </tr>
+  <tr>
+    <td>Test Device 1: J23101.BCD2.E0040.B0015<br>  (Colony 2)</td>
+    <td>0,0504</td>
+    <td>0,0492</td>
+    <td>0,0563</td>
+    <td>0,0686</td>
+    <td>297</td>
+    <td>301</td>
+    <td>287</td>
+    <td>294</td>
+    <td>0,00665</td>
+    <td>0,00545</td>
+    <td>0,01255</td>
+    <td>0,02485</td>
+    <td>71,25</td>
+    <td>75,25</td>
+    <td>61,25</td>
+    <td>68,25</td>
+    <td>1,70</td>
+    <td>2,19</td>
+    <td>0,77</td>
+    <td>0,44</td>
+    <td>1,27</td>
+    <td>0,81</td>
+    <td>1,06</td>
+    <td>2,094</td>
+  </tr>
+  <tr>
+    <td>Test Device 2: J23106.BCD2.E0040.B0015<br>  (Colony 1)</td>
+    <td>0,0493</td>
+    <td>0,0468</td>
+    <td>0,0503</td>
+    <td>0,0714</td>
+    <td>371</td>
+    <td>302</td>
+    <td>285</td>
+    <td>312</td>
+    <td>0,00555</td>
+    <td>0,00305</td>
+    <td>0,00655</td>
+    <td>0,02765</td>
+    <td>145,25</td>
+    <td>76,25</td>
+    <td>59,25</td>
+    <td>86,25</td>
+    <td>4,15</td>
+    <td>3,96</td>
+    <td>1,43</td>
+    <td>0,49</td>
+    <td>2,51</td>
+    <td>1,83</td>
+    <td>1,85</td>
+    <td>2,736</td>
+  </tr>
+  <tr>
+    <td>Test Device 2: J23106.BCD2.E0040.B0015<br>  (Colony 2)</td>
+    <td>0,0484</td>
+    <td>0,0485</td>
+    <td>0,0527</td>
+    <td>0,0617</td>
+    <td>287</td>
+    <td>311</td>
+    <td>285</td>
+    <td>303</td>
+    <td>0,00465</td>
+    <td>0,00475</td>
+    <td>0,00895</td>
+    <td>0,01795</td>
+    <td>61,25</td>
+    <td>85,25</td>
+    <td>59,25</td>
+    <td>77,25</td>
+    <td>2,09</td>
+    <td>2,85</td>
+    <td>1,05</td>
+    <td>0,68</td>
+    <td>1,67</td>
+    <td>0,99</td>
+    <td>1,44</td>
+    <td>1,912</td>
+  </tr>
+  <tr>
+    <td>Test Device 3: J23117.BCD2.E0040.B0015<br>  (Colony 1)</td>
+    <td>0,0483</td>
+    <td>0,0471</td>
+    <td>0,0518</td>
+    <td>0,0725</td>
+    <td>227</td>
+    <td>214</td>
+    <td>227</td>
+    <td>225</td>
+    <td>0,00455</td>
+    <td>0,00335</td>
+    <td>0,00805</td>
+    <td>0,02875</td>
+    <td>1,25</td>
+    <td>-11,75</td>
+    <td>1,25</td>
+    <td>-0,75</td>
+    <td>0,04</td>
+    <td>-0,56</td>
+    <td>0,02</td>
+    <td>0,00</td>
+    <td>-0,12</td>
+    <td>0,29</td>
+    <td></td>
+    <td></td>
+  </tr>
+  <tr>
+    <td>Test Device 3: J23117.BCD2.E0040.B0015<br>  (Colony 2)</td>
+    <td>0,0482</td>
+    <td>0,0473</td>
+    <td>0,052</td>
+    <td>0,0719</td>
+    <td>230</td>
+    <td>221</td>
+    <td>222</td>
+    <td>224</td>
+    <td>0,00445</td>
+    <td>0,00355</td>
+    <td>0,00825</td>
+    <td>0,02815</td>
+    <td>4,25</td>
+    <td>-4,75</td>
+    <td>-3,75</td>
+    <td>-1,75</td>
+    <td>0,15</td>
+    <td>-0,21</td>
+    <td>-0,07</td>
+    <td>-0,01</td>
+    <td>-0,04</td>
+    <td>0,15</td>
+    <td></td>
+    <td></td>
+  </tr>
+  <tr>
+    <td>Test Device 4: J23101+I13504 (Colony 1)</td>
+    <td>0,0486</td>
+    <td>0,0474</td>
+    <td>0,0513</td>
+    <td>0,0709</td>
+    <td>275</td>
+    <td>271</td>
+    <td>268</td>
+    <td>272</td>
+    <td>0,00485</td>
+    <td>0,00365</td>
+    <td>0,00755</td>
+    <td>0,02715</td>
+    <td>49,25</td>
+    <td>45,25</td>
+    <td>42,25</td>
+    <td>46,25</td>
+    <td>1,61</td>
+    <td>1,97</td>
+    <td>0,89</td>
+    <td>0,27</td>
+    <td>1,18</td>
+    <td>0,76</td>
+    <td>0,93</td>
+    <td>2,442</td>
+  </tr>
+  <tr>
+    <td>Test Device 4: J23101+I13504 (Colony 2)</td>
+    <td>0,0482</td>
+    <td>0,047</td>
+    <td>0,0523</td>
+    <td>0,0714</td>
+    <td>284</td>
+    <td>283</td>
+    <td>276</td>
+    <td>293</td>
+    <td>0,00445</td>
+    <td>0,00325</td>
+    <td>0,00855</td>
+    <td>0,02765</td>
+    <td>58,25</td>
+    <td>57,25</td>
+    <td>50,25</td>
+    <td>67,25</td>
+    <td>2,08</td>
+    <td>2,79</td>
+    <td>0,93</td>
+    <td>0,39</td>
+    <td>1,55</td>
+    <td>1,09</td>
+    <td>1,20</td>
+    <td>2,431</td>
+  </tr>
+  <tr>
+    <td>Test Device 5: J23106+I13504 (Colony 1)</td>
+    <td>0,0474</td>
+    <td>0,0459</td>
+    <td>0,0502</td>
+    <td>0,0596</td>
+    <td>249</td>
+    <td>237</td>
+    <td>231</td>
+    <td>244</td>
+    <td>0,00365</td>
+    <td>0,00215</td>
+    <td>0,00645</td>
+    <td>0,01585</td>
+    <td>23,25</td>
+    <td>11,25</td>
+    <td>5,25</td>
+    <td>18,25</td>
+    <td>1,01</td>
+    <td>0,83</td>
+    <td>0,13</td>
+    <td>0,18</td>
+    <td>0,54</td>
+    <td>0,45</td>
+    <td>0,37</td>
+    <td>2,840</td>
+  </tr>
+  <tr>
+    <td>Test Device 5: J23106+I13504 (Colony 2)</td>
+    <td>0,0496</td>
+    <td>0,0479</td>
+    <td>0,0539</td>
+    <td>0,0608</td>
+    <td>255</td>
+    <td>244</td>
+    <td>236</td>
+    <td>249</td>
+    <td>0,00585</td>
+    <td>0,00415</td>
+    <td>0,01015</td>
+    <td>0,01705</td>
+    <td>29,25</td>
+    <td>18,25</td>
+    <td>10,25</td>
+    <td>23,25</td>
+    <td>0,79</td>
+    <td>0,70</td>
+    <td>0,16</td>
+    <td>0,22</td>
+    <td>0,47</td>
+    <td>0,32</td>
+    <td>0,37</td>
+    <td>2,250</td>
+  </tr>
+  <tr>
+    <td>Test Device 6: J23117+I13504 (Colony 1)</td>
+    <td>0,0456</td>
+    <td>0,0442</td>
+    <td>0,0479</td>
+    <td>0,0624</td>
+    <td>230</td>
+    <td>230</td>
+    <td>219</td>
+    <td>232</td>
+    <td>0,00185</td>
+    <td>0,00045</td>
+    <td>0,00415</td>
+    <td>0,01865</td>
+    <td>4,25</td>
+    <td>4,25</td>
+    <td>-6,75</td>
+    <td>6,25</td>
+    <td>0,36</td>
+    <td>1,50</td>
+    <td>-0,26</td>
+    <td>0,05</td>
+    <td>0,41</td>
+    <td>0,77</td>
+    <td></td>
+    <td></td>
+  </tr>
+  <tr>
+    <td>Test Device 6: J23117+I13504 (Colony 2)</td>
+    <td>0,0482</td>
+    <td>0,0474</td>
+    <td>0,0533</td>
+    <td>0,0611</td>
+    <td>221</td>
+    <td>244</td>
+    <td>225</td>
+    <td>238</td>
+    <td>0,00445</td>
+    <td>0,00365</td>
+    <td>0,00955</td>
+    <td>0,01735</td>
+    <td>-4,75</td>
+    <td>18,25</td>
+    <td>-0,75</td>
+    <td>12,25</td>
+    <td>-0,17</td>
+    <td>0,79</td>
+    <td>-0,01</td>
+    <td>0,11</td>
+    <td>0,18</td>
+    <td>0,42</td>
+    <td></td>
+    <td></td>
+  </tr>
+  <tr>
+    <td>Hour 2:</td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+  </tr>
+  <tr>
+    <td>Negative Control (Colony 1)</td>
+    <td>0,0652</td>
+    <td>0,06</td>
+    <td>0,0671</td>
+    <td>0,081</td>
+    <td>234</td>
+    <td>229</td>
+    <td>230</td>
+    <td>227</td>
+    <td>0,02145</td>
+    <td>0,01625</td>
+    <td>0,02335</td>
+    <td>0,03725</td>
+    <td>8,25</td>
+    <td>3,25</td>
+    <td>4,25</td>
+    <td>1,25</td>
+    <td>0,06</td>
+    <td>0,03</td>
+    <td>0,03</td>
+    <td>0,01</td>
+    <td>0,03</td>
+    <td>0,02</td>
+    <td>0,02</td>
+    <td>2,830</td>
+  </tr>
+  <tr>
+    <td>Negative Control (Colony 2)</td>
+    <td>0,0667</td>
+    <td>0,0608</td>
+    <td>0,0642</td>
+    <td>0,0684</td>
+    <td>233</td>
+    <td>224</td>
+    <td>217</td>
+    <td>227</td>
+    <td>0,02295</td>
+    <td>0,01705</td>
+    <td>0,02045</td>
+    <td>0,02465</td>
+    <td>7,25</td>
+    <td>-1,75</td>
+    <td>-8,75</td>
+    <td>1,25</td>
+    <td>0,05</td>
+    <td>-0,02</td>
+    <td>-0,07</td>
+    <td>0,01</td>
+    <td>-0,01</td>
+    <td>0,05</td>
+    <td></td>
+    <td></td>
+  </tr>
+  <tr>
+    <td>Positive Control (Colony 1)</td>
+    <td>0,0568</td>
+    <td>0,0539</td>
+    <td>0,057</td>
+    <td>0,0695</td>
+    <td>841</td>
+    <td>850</td>
+    <td>741</td>
+    <td>758</td>
+    <td>0,01305</td>
+    <td>0,01015</td>
+    <td>0,01325</td>
+    <td>0,02575</td>
+    <td>615,25</td>
+    <td>624,25</td>
+    <td>515,25</td>
+    <td>532,25</td>
+    <td>7,47</td>
+    <td>9,75</td>
+    <td>6,17</td>
+    <td>3,28</td>
+    <td>6,67</td>
+    <td>2,70</td>
+    <td>6,19</td>
+    <td>1,591</td>
+  </tr>
+  <tr>
+    <td>Positive Control (Colony 2)</td>
+    <td>0,0602</td>
+    <td>0,0558</td>
+    <td>0,0614</td>
+    <td>0,057</td>
+    <td>913</td>
+    <td>784</td>
+    <td>712</td>
+    <td>706</td>
+    <td>0,01645</td>
+    <td>0,01205</td>
+    <td>0,01765</td>
+    <td>0,01325</td>
+    <td>687,25</td>
+    <td>558,25</td>
+    <td>486,25</td>
+    <td>480,25</td>
+    <td>6,62</td>
+    <td>7,35</td>
+    <td>4,37</td>
+    <td>5,75</td>
+    <td>6,02</td>
+    <td>1,28</td>
+    <td>5,91</td>
+    <td>1,253</td>
+  </tr>
+  <tr>
+    <td>Test Device 1: J23101.BCD2.E0040.B0015<br>  (Colony 1)</td>
+    <td>0,0599</td>
+    <td>0,0569</td>
+    <td>0,0626</td>
+    <td>0,082</td>
+    <td>916</td>
+    <td>864</td>
+    <td>810</td>
+    <td>942</td>
+    <td>0,01615</td>
+    <td>0,01315</td>
+    <td>0,01885</td>
+    <td>0,03825</td>
+    <td>690,25</td>
+    <td>638,25</td>
+    <td>584,25</td>
+    <td>716,25</td>
+    <td>6,78</td>
+    <td>7,70</td>
+    <td>4,91</td>
+    <td>2,97</td>
+    <td>5,59</td>
+    <td>2,09</td>
+    <td>5,25</td>
+    <td>1,529</td>
+  </tr>
+  <tr>
+    <td>Test Device 1: J23101.BCD2.E0040.B0015<br>  (Colony 2)</td>
+    <td>0,062</td>
+    <td>0,0568</td>
+    <td>0,0624</td>
+    <td>0,071</td>
+    <td>1016</td>
+    <td>878</td>
+    <td>765</td>
+    <td>748</td>
+    <td>0,01825</td>
+    <td>0,01305</td>
+    <td>0,01865</td>
+    <td>0,02725</td>
+    <td>790,25</td>
+    <td>652,25</td>
+    <td>539,25</td>
+    <td>522,25</td>
+    <td>6,87</td>
+    <td>7,92</td>
+    <td>4,58</td>
+    <td>3,04</td>
+    <td>5,60</td>
+    <td>2,21</td>
+    <td>5,25</td>
+    <td>1,540</td>
+  </tr>
+  <tr>
+    <td>Test Device 2: J23106.BCD2.E0040.B0015<br>  (Colony 1)</td>
+    <td>0,0613</td>
+    <td>0,057</td>
+    <td>0,0637</td>
+    <td>0,0799</td>
+    <td>973</td>
+    <td>923</td>
+    <td>897</td>
+    <td>893</td>
+    <td>0,01755</td>
+    <td>0,01325</td>
+    <td>0,01995</td>
+    <td>0,03615</td>
+    <td>747,25</td>
+    <td>697,25</td>
+    <td>671,25</td>
+    <td>667,25</td>
+    <td>6,75</td>
+    <td>8,34</td>
+    <td>5,33</td>
+    <td>2,93</td>
+    <td>5,84</td>
+    <td>2,30</td>
+    <td>5,45</td>
+    <td>1,572</td>
+  </tr>
+  <tr>
+    <td>Test Device 2: J23106.BCD2.E0040.B0015<br>  (Colony 2)</td>
+    <td>0,065</td>
+    <td>0,0592</td>
+    <td>0,0669</td>
+    <td>0,0803</td>
+    <td>1045</td>
+    <td>893</td>
+    <td>877</td>
+    <td>904</td>
+    <td>0,02125</td>
+    <td>0,01545</td>
+    <td>0,02315</td>
+    <td>0,03655</td>
+    <td>819,25</td>
+    <td>667,25</td>
+    <td>651,25</td>
+    <td>678,25</td>
+    <td>6,11</td>
+    <td>6,85</td>
+    <td>4,46</td>
+    <td>2,94</td>
+    <td>5,09</td>
+    <td>1,75</td>
+    <td>4,84</td>
+    <td>1,460</td>
+  </tr>
+  <tr>
+    <td>Test Device 3: J23117.BCD2.E0040.B0015<br>  (Colony 1)</td>
+    <td>0,068</td>
+    <td>0,0632</td>
+    <td>0,0671</td>
+    <td>0,0833</td>
+    <td>241</td>
+    <td>234</td>
+    <td>236</td>
+    <td>240</td>
+    <td>0,02425</td>
+    <td>0,01945</td>
+    <td>0,02335</td>
+    <td>0,03955</td>
+    <td>15,25</td>
+    <td>8,25</td>
+    <td>10,25</td>
+    <td>14,25</td>
+    <td>0,10</td>
+    <td>0,07</td>
+    <td>0,07</td>
+    <td>0,06</td>
+    <td>0,07</td>
+    <td>0,02</td>
+    <td>0,07</td>
+    <td>1,265</td>
+  </tr>
+  <tr>
+    <td>Test Device 3: J23117.BCD2.E0040.B0015<br>  (Colony 2)</td>
+    <td>0,0704</td>
+    <td>0,0654</td>
+    <td>0,0719</td>
+    <td>0,0959</td>
+    <td>246</td>
+    <td>243</td>
+    <td>228</td>
+    <td>237</td>
+    <td>0,02665</td>
+    <td>0,02165</td>
+    <td>0,02815</td>
+    <td>0,05215</td>
+    <td>20,25</td>
+    <td>17,25</td>
+    <td>2,25</td>
+    <td>11,25</td>
+    <td>0,12</td>
+    <td>0,13</td>
+    <td>0,01</td>
+    <td>0,03</td>
+    <td>0,07</td>
+    <td>0,06</td>
+    <td>0,05</td>
+    <td>3,018</td>
+  </tr>
+  <tr>
+    <td>Test Device 4: J23101+I13504 (Colony 1)</td>
+    <td>0,0521</td>
+    <td>0,0496</td>
+    <td>0,0547</td>
+    <td>0,0674</td>
+    <td>640</td>
+    <td>608</td>
+    <td>551</td>
+    <td>564</td>
+    <td>0,00835</td>
+    <td>0,00585</td>
+    <td>0,01095</td>
+    <td>0,02365</td>
+    <td>414,25</td>
+    <td>382,25</td>
+    <td>325,25</td>
+    <td>338,25</td>
+    <td>7,87</td>
+    <td>10,36</td>
+    <td>4,71</td>
+    <td>2,27</td>
+    <td>6,30</td>
+    <td>3,55</td>
+    <td>5,43</td>
+    <td>1,950</td>
+  </tr>
+  <tr>
+    <td>Test Device 4: J23101+I13504 (Colony 2)</td>
+    <td>0,0499</td>
+    <td>0,052</td>
+    <td>0,0584</td>
+    <td>0,0653</td>
+    <td>521</td>
+    <td>702</td>
+    <td>597</td>
+    <td>598</td>
+    <td>0,00615</td>
+    <td>0,00825</td>
+    <td>0,01465</td>
+    <td>0,02155</td>
+    <td>295,25</td>
+    <td>476,25</td>
+    <td>371,25</td>
+    <td>372,25</td>
+    <td>7,61</td>
+    <td>9,15</td>
+    <td>4,02</td>
+    <td>2,74</td>
+    <td>5,88</td>
+    <td>3,00</td>
+    <td>5,26</td>
+    <td>1,751</td>
+  </tr>
+  <tr>
+    <td>Test Device 5: J23106+I13504 (Colony 1)</td>
+    <td>0,0742</td>
+    <td>0,0671</td>
+    <td>0,0698</td>
+    <td>0,0836</td>
+    <td>740</td>
+    <td>641</td>
+    <td>545</td>
+    <td>613</td>
+    <td>0,03045</td>
+    <td>0,02335</td>
+    <td>0,02605</td>
+    <td>0,03985</td>
+    <td>514,25</td>
+    <td>415,25</td>
+    <td>319,25</td>
+    <td>387,25</td>
+    <td>2,68</td>
+    <td>2,82</td>
+    <td>1,94</td>
+    <td>1,54</td>
+    <td>2,25</td>
+    <td>0,61</td>
+    <td>2,18</td>
+    <td>1,329</td>
+  </tr>
+  <tr>
+    <td>Test Device 5: J23106+I13504 (Colony 2)</td>
+    <td>0,0729</td>
+    <td>0,068</td>
+    <td>0,0732</td>
+    <td>0,0843</td>
+    <td>661</td>
+    <td>642</td>
+    <td>571</td>
+    <td>576</td>
+    <td>0,02915</td>
+    <td>0,02425</td>
+    <td>0,02945</td>
+    <td>0,04055</td>
+    <td>435,25</td>
+    <td>416,25</td>
+    <td>345,25</td>
+    <td>350,25</td>
+    <td>2,37</td>
+    <td>2,72</td>
+    <td>1,86</td>
+    <td>1,37</td>
+    <td>2,08</td>
+    <td>0,59</td>
+    <td>2,01</td>
+    <td>1,351</td>
+  </tr>
+  <tr>
+    <td>Test Device 6: J23117+I13504 (Colony 1)</td>
+    <td>0,075</td>
+    <td>0,0713</td>
+    <td>0,0754</td>
+    <td>0,085</td>
+    <td>242</td>
+    <td>239</td>
+    <td>254</td>
+    <td>241</td>
+    <td>0,03125</td>
+    <td>0,02755</td>
+    <td>0,03165</td>
+    <td>0,04125</td>
+    <td>16,25</td>
+    <td>13,25</td>
+    <td>28,25</td>
+    <td>15,25</td>
+    <td>0,08</td>
+    <td>0,08</td>
+    <td>0,14</td>
+    <td>0,06</td>
+    <td>0,09</td>
+    <td>0,04</td>
+    <td>0,08</td>
+    <td>1,448</td>
+  </tr>
+  <tr>
+    <td>Test Device 6: J23117+I13504 (Colony 2)</td>
+    <td>0,0774</td>
+    <td>0,0721</td>
+    <td>0,0676</td>
+    <td>0,0848</td>
+    <td>244</td>
+    <td>233</td>
+    <td>219</td>
+    <td>252</td>
+    <td>0,03365</td>
+    <td>0,02835</td>
+    <td>0,02385</td>
+    <td>0,04105</td>
+    <td>18,25</td>
+    <td>7,25</td>
+    <td>-6,75</td>
+    <td>26,25</td>
+    <td>0,09</td>
+    <td>0,04</td>
+    <td>-0,04</td>
+    <td>0,10</td>
+    <td>0,05</td>
+    <td>0,07</td>
+    <td></td>
+    <td></td>
+  </tr>
+  <tr>
+    <td>Hour 4:</td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+  </tr>
+  <tr>
+    <td>Negative Control (Colony 1)</td>
+    <td>0,1765</td>
+    <td>0,1804</td>
+    <td>0,1321</td>
+    <td>0,1354</td>
+    <td>265</td>
+    <td>269</td>
+    <td>245</td>
+    <td>247</td>
+    <td>0,13275</td>
+    <td>0,13665</td>
+    <td>0,08835</td>
+    <td>0,09165</td>
+    <td>39,25</td>
+    <td>43,25</td>
+    <td>19,25</td>
+    <td>21,25</td>
+    <td>0,05</td>
+    <td>0,05</td>
+    <td>0,03</td>
+    <td>0,04</td>
+    <td>0,04</td>
+    <td>0,01</td>
+    <td>0,04</td>
+    <td>1,199</td>
+  </tr>
+  <tr>
+    <td>Negative Control (Colony 2)</td>
+    <td>0,3007</td>
+    <td>0,1467</td>
+    <td>0,1374</td>
+    <td>0,1423</td>
+    <td>269</td>
+    <td>253</td>
+    <td>249</td>
+    <td>261</td>
+    <td>0,25695</td>
+    <td>0,10295</td>
+    <td>0,09365</td>
+    <td>0,09855</td>
+    <td>43,25</td>
+    <td>27,25</td>
+    <td>23,25</td>
+    <td>35,25</td>
+    <td>0,03</td>
+    <td>0,04</td>
+    <td>0,04</td>
+    <td>0,06</td>
+    <td>0,04</td>
+    <td>0,01</td>
+    <td>0,04</td>
+    <td>1,363</td>
+  </tr>
+  <tr>
+    <td>Positive Control (Colony 1)</td>
+    <td>0,1449</td>
+    <td>0,1515</td>
+    <td>0,1194</td>
+    <td>0,1348</td>
+    <td>3725</td>
+    <td>3980</td>
+    <td>2579</td>
+    <td>2934</td>
+    <td>0,10115</td>
+    <td>0,10775</td>
+    <td>0,07565</td>
+    <td>0,09105</td>
+    <td>3499,25</td>
+    <td>3754,25</td>
+    <td>2353,25</td>
+    <td>2708,25</td>
+    <td>5,48</td>
+    <td>5,52</td>
+    <td>4,93</td>
+    <td>4,72</td>
+    <td>5,16</td>
+    <td>0,40</td>
+    <td>5,15</td>
+    <td>1,082</td>
+  </tr>
+  <tr>
+    <td>Positive Control (Colony 2)</td>
+    <td>0,2746</td>
+    <td>0,1192</td>
+    <td>0,1356</td>
+    <td>0,1255</td>
+    <td>3980</td>
+    <td>2825</td>
+    <td>3397</td>
+    <td>2585</td>
+    <td>0,23085</td>
+    <td>0,07545</td>
+    <td>0,09185</td>
+    <td>0,08175</td>
+    <td>3754,25</td>
+    <td>2599,25</td>
+    <td>3171,25</td>
+    <td>2359,25</td>
+    <td>2,58</td>
+    <td>5,46</td>
+    <td>5,47</td>
+    <td>4,58</td>
+    <td>4,52</td>
+    <td>1,36</td>
+    <td>4,33</td>
+    <td>1,428</td>
+  </tr>
+  <tr>
+    <td>Test Device 1: J23101.BCD2.E0040.B0015<br>  (Colony 1)</td>
+    <td>0,1431</td>
+    <td>0,1365</td>
+    <td>0,1199</td>
+    <td>0,1296</td>
+    <td>4330</td>
+    <td>3936</td>
+    <td>3110</td>
+    <td>3253</td>
+    <td>0,09935</td>
+    <td>0,09275</td>
+    <td>0,07615</td>
+    <td>0,08585</td>
+    <td>4104,25</td>
+    <td>3710,25</td>
+    <td>2884,25</td>
+    <td>3027,25</td>
+    <td>6,55</td>
+    <td>6,34</td>
+    <td>6,01</td>
+    <td>5,59</td>
+    <td>6,12</td>
+    <td>0,42</td>
+    <td>6,11</td>
+    <td>1,072</td>
+  </tr>
+  <tr>
+    <td>Test Device 1: J23101.BCD2.E0040.B0015<br>  (Colony 2)</td>
+    <td>0,2586</td>
+    <td>0,1135</td>
+    <td>0,1199</td>
+    <td>0,138</td>
+    <td>3936</td>
+    <td>3064</td>
+    <td>3055</td>
+    <td>3302</td>
+    <td>0,21485</td>
+    <td>0,06975</td>
+    <td>0,07615</td>
+    <td>0,09425</td>
+    <td>3710,25</td>
+    <td>2838,25</td>
+    <td>2829,25</td>
+    <td>3076,25</td>
+    <td>2,74</td>
+    <td>6,45</td>
+    <td>5,89</td>
+    <td>5,17</td>
+    <td>5,06</td>
+    <td>1,64</td>
+    <td>4,82</td>
+    <td>1,473</td>
+  </tr>
+  <tr>
+    <td>Test Device 2: J23106.BCD2.E0040.B0015<br>  (Colony 1)</td>
+    <td>0,1661</td>
+    <td>0,1585</td>
+    <td>0,132</td>
+    <td>0,1441</td>
+    <td>4534</td>
+    <td>4087</td>
+    <td>3080</td>
+    <td>3214</td>
+    <td>0,12235</td>
+    <td>0,11475</td>
+    <td>0,08825</td>
+    <td>0,10035</td>
+    <td>4308,25</td>
+    <td>3861,25</td>
+    <td>2854,25</td>
+    <td>2988,25</td>
+    <td>5,58</td>
+    <td>5,33</td>
+    <td>5,13</td>
+    <td>4,72</td>
+    <td>5,19</td>
+    <td>0,36</td>
+    <td>5,18</td>
+    <td>1,074</td>
+  </tr>
+  <tr>
+    <td>Test Device 2: J23106.BCD2.E0040.B0015<br>  (Colony 2)</td>
+    <td>0,308</td>
+    <td>0,1373</td>
+    <td>0,1344</td>
+    <td>0,1527</td>
+    <td>4087</td>
+    <td>3547</td>
+    <td>3125</td>
+    <td>3124</td>
+    <td>0,26425</td>
+    <td>0,09355</td>
+    <td>0,09065</td>
+    <td>0,10895</td>
+    <td>3861,25</td>
+    <td>3321,25</td>
+    <td>2899,25</td>
+    <td>2898,25</td>
+    <td>2,32</td>
+    <td>5,63</td>
+    <td>5,07</td>
+    <td>4,22</td>
+    <td>4,31</td>
+    <td>1,45</td>
+    <td>4,09</td>
+    <td>1,487</td>
+  </tr>
+  <tr>
+    <td>Test Device 3: J23117.BCD2.E0040.B0015<br>  (Colony 1)</td>
+    <td>0,1677</td>
+    <td>0,1861</td>
+    <td>0,1482</td>
+    <td>0,1762</td>
+    <td>327</td>
+    <td>334</td>
+    <td>300</td>
+    <td>313</td>
+    <td>0,12395</td>
+    <td>0,14235</td>
+    <td>0,10445</td>
+    <td>0,13245</td>
+    <td>101,25</td>
+    <td>108,25</td>
+    <td>74,25</td>
+    <td>87,25</td>
+    <td>0,13</td>
+    <td>0,12</td>
+    <td>0,11</td>
+    <td>0,10</td>
+    <td>0,12</td>
+    <td>0,01</td>
+    <td>0,12</td>
+    <td>1,096</td>
+  </tr>
+  <tr>
+    <td>Test Device 3: J23117.BCD2.E0040.B0015<br>  (Colony 2)</td>
+    <td>0,3246</td>
+    <td>0,1643</td>
+    <td>0,1453</td>
+    <td>0,1686</td>
+    <td>334</td>
+    <td>321</td>
+    <td>309</td>
+    <td>322</td>
+    <td>0,28085</td>
+    <td>0,12055</td>
+    <td>0,10155</td>
+    <td>0,12485</td>
+    <td>108,25</td>
+    <td>95,25</td>
+    <td>83,25</td>
+    <td>96,25</td>
+    <td>0,06</td>
+    <td>0,13</td>
+    <td>0,13</td>
+    <td>0,12</td>
+    <td>0,11</td>
+    <td>0,03</td>
+    <td>0,11</td>
+    <td>1,436</td>
+  </tr>
+  <tr>
+    <td>Test Device 4: J23101+I13504 (Colony 1)</td>
+    <td>0,0692</td>
+    <td>0,07</td>
+    <td>0,0666</td>
+    <td>0,0885</td>
+    <td>1619</td>
+    <td>1489</td>
+    <td>1280</td>
+    <td>1543</td>
+    <td>0,02545</td>
+    <td>0,02625</td>
+    <td>0,02285</td>
+    <td>0,04475</td>
+    <td>1393,25</td>
+    <td>1263,25</td>
+    <td>1054,25</td>
+    <td>1317,25</td>
+    <td>8,68</td>
+    <td>7,63</td>
+    <td>7,31</td>
+    <td>4,67</td>
+    <td>7,07</td>
+    <td>1,71</td>
+    <td>6,90</td>
+    <td>1,310</td>
+  </tr>
+  <tr>
+    <td>Test Device 4: J23101+I13504 (Colony 2)</td>
+    <td>0,339</td>
+    <td>0,0698</td>
+    <td>0,0763</td>
+    <td>0,0765</td>
+    <td>1489</td>
+    <td>1619</td>
+    <td>1516</td>
+    <td>1589</td>
+    <td>0,29525</td>
+    <td>0,02605</td>
+    <td>0,03255</td>
+    <td>0,03275</td>
+    <td>1263,25</td>
+    <td>1393,25</td>
+    <td>1290,25</td>
+    <td>1363,25</td>
+    <td>0,68</td>
+    <td>8,48</td>
+    <td>6,28</td>
+    <td>6,60</td>
+    <td>5,51</td>
+    <td>3,36</td>
+    <td>3,93</td>
+    <td>3,250</td>
+  </tr>
+  <tr>
+    <td>Test Device 5: J23106+I13504 (Colony 1)</td>
+    <td>0,1766</td>
+    <td>0,19</td>
+    <td>0,1573</td>
+    <td>0,164</td>
+    <td>1493</td>
+    <td>1551</td>
+    <td>1257</td>
+    <td>1358</td>
+    <td>0,13285</td>
+    <td>0,14625</td>
+    <td>0,11355</td>
+    <td>0,12025</td>
+    <td>1267,25</td>
+    <td>1325,25</td>
+    <td>1031,25</td>
+    <td>1132,25</td>
+    <td>1,51</td>
+    <td>1,44</td>
+    <td>1,44</td>
+    <td>1,49</td>
+    <td>1,47</td>
+    <td>0,04</td>
+    <td>1,47</td>
+    <td>1,026</td>
+  </tr>
+  <tr>
+    <td>Test Device 5: J23106+I13504 (Colony 2)</td>
+    <td>0,3438</td>
+    <td>0,151</td>
+    <td>0,1532</td>
+    <td>0,1522</td>
+    <td>1551</td>
+    <td>1294</td>
+    <td>1238</td>
+    <td>1285</td>
+    <td>0,30005</td>
+    <td>0,10725</td>
+    <td>0,10945</td>
+    <td>0,10845</td>
+    <td>1325,25</td>
+    <td>1068,25</td>
+    <td>1012,25</td>
+    <td>1059,25</td>
+    <td>0,70</td>
+    <td>1,58</td>
+    <td>1,47</td>
+    <td>1,55</td>
+    <td>1,32</td>
+    <td>0,42</td>
+    <td>1,26</td>
+    <td>1,480</td>
+  </tr>
+  <tr>
+    <td>Test Device 6: J23117+I13504 (Colony 1)</td>
+    <td>0,193</td>
+    <td>0,167</td>
+    <td>0,1578</td>
+    <td>0,189</td>
+    <td>293</td>
+    <td>275</td>
+    <td>276</td>
+    <td>304</td>
+    <td>0,14925</td>
+    <td>0,12325</td>
+    <td>0,11405</td>
+    <td>0,14525</td>
+    <td>67,25</td>
+    <td>49,25</td>
+    <td>50,25</td>
+    <td>78,25</td>
+    <td>0,07</td>
+    <td>0,06</td>
+    <td>0,07</td>
+    <td>0,09</td>
+    <td>0,07</td>
+    <td>0,01</td>
+    <td>0,07</td>
+    <td>1,133</td>
+  </tr>
+  <tr>
+    <td>Test Device 6: J23117+I13504 (Colony 2)</td>
+    <td>0,3446</td>
+    <td>0,1849</td>
+    <td>0,1626</td>
+    <td>0,1622</td>
+    <td>275</td>
+    <td>308</td>
+    <td>276</td>
+    <td>292</td>
+    <td>0,30085</td>
+    <td>0,14115</td>
+    <td>0,11885</td>
+    <td>0,11845</td>
+    <td>49,25</td>
+    <td>82,25</td>
+    <td>50,25</td>
+    <td>66,25</td>
+    <td>0,03</td>
+    <td>0,09</td>
+    <td>0,07</td>
+    <td>0,09</td>
+    <td>0,07</td>
+    <td>0,03</td>
+    <td>0,06</td>
+    <td>1,807</td>
+  </tr>
+  <tr>
+    <td>Hour 6:</td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+    <td></td>
+  </tr>
+  <tr>
+    <td>Negative Control (Colony 1)</td>
+    <td>0,2911</td>
+    <td>0,2735</td>
+    <td>0,2694</td>
+    <td>0,3314</td>
+    <td>289</td>
+    <td>290</td>
+    <td>284</td>
+    <td>320</td>
+    <td>0,24735</td>
+    <td>0,22975</td>
+    <td>0,22565</td>
+    <td>0,28765</td>
+    <td>63,25</td>
+    <td>64,25</td>
+    <td>58,25</td>
+    <td>94,25</td>
+    <td>0,04</td>
+    <td>0,04</td>
+    <td>0,04</td>
+    <td>0,05</td>
+    <td>0,04</td>
+    <td>0,01</td>
+    <td>0,04</td>
+    <td>1,122</td>
+  </tr>
+  <tr>
+    <td>Negative Control (Colony 2)</td>
+    <td>0,3007</td>
+    <td>0,2853</td>
+    <td>0,282</td>
+    <td>0,2851</td>
+    <td>285</td>
+    <td>286</td>
+    <td>285</td>
+    <td>294</td>
+    <td>0,25695</td>
+    <td>0,24155</td>
+    <td>0,23825</td>
+    <td>0,24135</td>
+    <td>59,25</td>
+    <td>60,25</td>
+    <td>59,25</td>
+    <td>68,25</td>
+    <td>0,04</td>
+    <td>0,04</td>
+    <td>0,04</td>
+    <td>0,04</td>
+    <td>0,04</td>
+    <td>0,00</td>
+    <td>0,04</td>
+    <td>1,088</td>
+  </tr>
+  <tr>
+    <td>Positive Control (Colony 1)</td>
+    <td>0,2572</td>
+    <td>0,2467</td>
+    <td>0,2542</td>
+    <td>0,2762</td>
+    <td>7046</td>
+    <td>7161</td>
+    <td>6900</td>
+    <td>7664</td>
+    <td>0,21345</td>
+    <td>0,20295</td>
+    <td>0,21045</td>
+    <td>0,23245</td>
+    <td>6820,25</td>
+    <td>6935,25</td>
+    <td>6674,25</td>
+    <td>7438,25</td>
+    <td>5,07</td>
+    <td>5,42</td>
+    <td>5,03</td>
+    <td>5,07</td>
+    <td>5,15</td>
+    <td>0,18</td>
+    <td>5,14</td>
+    <td>1,035</td>
+  </tr>
+  <tr>
+    <td>Positive Control (Colony 2)</td>
+    <td>0,2746</td>
+    <td>0,2574</td>
+    <td>0,2546</td>
+    <td>0,2633</td>
+    <td>7246</td>
+    <td>7418</td>
+    <td>6939</td>
+    <td>7882</td>
+    <td>0,23085</td>
+    <td>0,21365</td>
+    <td>0,21085</td>
+    <td>0,21955</td>
+    <td>7020,25</td>
+    <td>7192,25</td>
+    <td>6713,25</td>
+    <td>7656,25</td>
+    <td>4,82</td>
+    <td>5,34</td>
+    <td>5,05</td>
+    <td>5,53</td>
+    <td>5,18</td>
+    <td>0,31</td>
+    <td>5,18</td>
+    <td>1,062</td>
+  </tr>
+  <tr>
+    <td>Test Device 1: J23101.BCD2.E0040.B0015<br>  (Colony 1)</td>
+    <td>0,2697</td>
+    <td>0,2558</td>
+    <td>0,2557</td>
+    <td>0,2711</td>
+    <td>9753</td>
+    <td>9621</td>
+    <td>8983</td>
+    <td>10384</td>
+    <td>0,22595</td>
+    <td>0,21205</td>
+    <td>0,21195</td>
+    <td>0,22735</td>
+    <td>9527,25</td>
+    <td>9395,25</td>
+    <td>8757,25</td>
+    <td>10158,25</td>
+    <td>6,69</td>
+    <td>7,02</td>
+    <td>6,55</td>
+    <td>7,08</td>
+    <td>6,84</td>
+    <td>0,26</td>
+    <td>6,83</td>
+    <td>1,039</td>
+  </tr>
+  <tr>
+    <td>Test Device 1: J23101.BCD2.E0040.B0015<br>  (Colony 2)</td>
+    <td>0,2586</td>
+    <td>0,2783</td>
+    <td>0,2629</td>
+    <td>0,2614</td>
+    <td>9059</td>
+    <td>10409</td>
+    <td>9332</td>
+    <td>9938</td>
+    <td>0,21485</td>
+    <td>0,23455</td>
+    <td>0,21915</td>
+    <td>0,21765</td>
+    <td>8833,25</td>
+    <td>10183,25</td>
+    <td>9106,25</td>
+    <td>9712,25</td>
+    <td>6,52</td>
+    <td>6,88</td>
+    <td>6,59</td>
+    <td>7,07</td>
+    <td>6,77</td>
+    <td>0,26</td>
+    <td>6,76</td>
+    <td>1,039</td>
+  </tr>
+  <tr>
+    <td>Test Device 2: J23106.BCD2.E0040.B0015<br>  (Colony 1)</td>
+    <td>0,2843</td>
+    <td>0,2596</td>
+    <td>0,2671</td>
+    <td>0,2787</td>
+    <td>10223</td>
+    <td>9685</td>
+    <td>9620</td>
+    <td>10759</td>
+    <td>0,24055</td>
+    <td>0,21585</td>
+    <td>0,22335</td>
+    <td>0,23495</td>
+    <td>9997,25</td>
+    <td>9459,25</td>
+    <td>9394,25</td>
+    <td>10533,25</td>
+    <td>6,59</td>
+    <td>6,95</td>
+    <td>6,67</td>
+    <td>7,11</td>
+    <td>6,83</td>
+    <td>0,24</td>
+    <td>6,83</td>
+    <td>1,036</td>
+  </tr>
+  <tr>
+    <td>Test Device 2: J23106.BCD2.E0040.B0015<br>  (Colony 2)</td>
+    <td>0,308</td>
+    <td>0,2668</td>
+    <td>0,2691</td>
+    <td>0,2668</td>
+    <td>10726</td>
+    <td>10079</td>
+    <td>10013</td>
+    <td>10980</td>
+    <td>0,26425</td>
+    <td>0,22305</td>
+    <td>0,22535</td>
+    <td>0,22305</td>
+    <td>10500,25</td>
+    <td>9853,25</td>
+    <td>9787,25</td>
+    <td>10754,25</td>
+    <td>6,30</td>
+    <td>7,00</td>
+    <td>6,89</td>
+    <td>7,64</td>
+    <td>6,96</td>
+    <td>0,55</td>
+    <td>6,94</td>
+    <td>1,082</td>
+  </tr>
+  <tr>
+    <td>Test Device 3: J23117.BCD2.E0040.B0015<br>  (Colony 1)</td>
+    <td>0,311</td>
+    <td>0,2984</td>
+    <td>0,2856</td>
+    <td>0,2999</td>
+    <td>429</td>
+    <td>429</td>
+    <td>420</td>
+    <td>441</td>
+    <td>0,26725</td>
+    <td>0,25465</td>
+    <td>0,24185</td>
+    <td>0,25615</td>
+    <td>203,25</td>
+    <td>203,25</td>
+    <td>194,25</td>
+    <td>215,25</td>
+    <td>0,12</td>
+    <td>0,13</td>
+    <td>0,13</td>
+    <td>0,13</td>
+    <td>0,13</td>
+    <td>0,01</td>
+    <td>0,13</td>
+    <td>1,042</td>
+  </tr>
+  <tr>
+    <td>Test Device 3: J23117.BCD2.E0040.B0015<br>  (Colony 2)</td>
+    <td>0,3246</td>
+    <td>0,2989</td>
+    <td>0,3006</td>
+    <td>0,2911</td>
+    <td>445</td>
+    <td>442</td>
+    <td>439</td>
+    <td>446</td>
+    <td>0,28085</td>
+    <td>0,25515</td>
+    <td>0,25685</td>
+    <td>0,24735</td>
+    <td>219,25</td>
+    <td>216,25</td>
+    <td>213,25</td>
+    <td>220,25</td>
+    <td>0,12</td>
+    <td>0,13</td>
+    <td>0,13</td>
+    <td>0,14</td>
+    <td>0,13</td>
+    <td>0,01</td>
+    <td>0,13</td>
+    <td>1,056</td>
+  </tr>
+  <tr>
+    <td>Test Device 4: J23101+I13504 (Colony 1)</td>
+    <td>0,1557</td>
+    <td>0,1439</td>
+    <td>0,1464</td>
+    <td>0,1738</td>
+    <td>4924</td>
+    <td>4659</td>
+    <td>4563</td>
+    <td>5092</td>
+    <td>0,11195</td>
+    <td>0,10015</td>
+    <td>0,10265</td>
+    <td>0,13005</td>
+    <td>4698,25</td>
+    <td>4433,25</td>
+    <td>4337,25</td>
+    <td>4866,25</td>
+    <td>6,65</td>
+    <td>7,02</td>
+    <td>6,70</td>
+    <td>5,93</td>
+    <td>6,58</td>
+    <td>0,46</td>
+    <td>6,56</td>
+    <td>1,074</td>
+  </tr>
+  <tr>
+    <td>Test Device 4: J23101+I13504 (Colony 2)</td>
+    <td>0,339</td>
+    <td>0,3188</td>
+    <td>0,3064</td>
+    <td>0,3239</td>
+    <td>2372</td>
+    <td>2357</td>
+    <td>2297</td>
+    <td>2523</td>
+    <td>0,29525</td>
+    <td>0,27505</td>
+    <td>0,26265</td>
+    <td>0,28015</td>
+    <td>2146,25</td>
+    <td>2131,25</td>
+    <td>2071,25</td>
+    <td>2297,25</td>
+    <td>1,15</td>
+    <td>1,23</td>
+    <td>1,25</td>
+    <td>1,30</td>
+    <td>1,23</td>
+    <td>0,06</td>
+    <td>1,23</td>
+    <td>1,052</td>
+  </tr>
+  <tr>
+    <td>Test Device 5: J23106+I13504 (Colony 1)</td>
+    <td>0,1581</td>
+    <td>0,1471</td>
+    <td>0,1531</td>
+    <td>0,1763</td>
+    <td>5031</td>
+    <td>4916</td>
+    <td>4830</td>
+    <td>5472</td>
+    <td>0,11435</td>
+    <td>0,10335</td>
+    <td>0,10935</td>
+    <td>0,13255</td>
+    <td>4805,25</td>
+    <td>4690,25</td>
+    <td>4604,25</td>
+    <td>5246,25</td>
+    <td>6,66</td>
+    <td>7,20</td>
+    <td>6,68</td>
+    <td>6,28</td>
+    <td>6,70</td>
+    <td>0,38</td>
+    <td>6,69</td>
+    <td>1,058</td>
+  </tr>
+  <tr>
+    <td>Test Device 5: J23106+I13504 (Colony 2)</td>
+    <td>0,3438</td>
+    <td>0,3134</td>
+    <td>0,3063</td>
+    <td>0,336</td>
+    <td>2368</td>
+    <td>2321</td>
+    <td>2299</td>
+    <td>2513</td>
+    <td>0,30005</td>
+    <td>0,26965</td>
+    <td>0,26255</td>
+    <td>0,29225</td>
+    <td>2142,25</td>
+    <td>2095,25</td>
+    <td>2073,25</td>
+    <td>2287,25</td>
+    <td>1,13</td>
+    <td>1,23</td>
+    <td>1,25</td>
+    <td>1,24</td>
+    <td>1,21</td>
+    <td>0,06</td>
+    <td>1,21</td>
+    <td>1,048</td>
+  </tr>
+  <tr>
+    <td>Test Device 6: J23117+I13504 (Colony 1)</td>
+    <td>0,3271</td>
+    <td>0,3082</td>
+    <td>0,3093</td>
+    <td>0,2938</td>
+    <td>328</td>
+    <td>322</td>
+    <td>335</td>
+    <td>341</td>
+    <td>0,28335</td>
+    <td>0,26445</td>
+    <td>0,26555</td>
+    <td>0,25005</td>
+    <td>102,25</td>
+    <td>96,25</td>
+    <td>109,25</td>
+    <td>115,25</td>
+    <td>0,06</td>
+    <td>0,06</td>
+    <td>0,07</td>
+    <td>0,07</td>
+    <td>0,06</td>
+    <td>0,01</td>
+    <td>0,06</td>
+    <td>1,123</td>
+  </tr>
+  <tr>
+    <td>Test Device 6: J23117+I13504 (Colony 2)</td>
+    <td>0,3446</td>
+    <td>0,3161</td>
+    <td>0,3094</td>
+    <td>0,3191</td>
+    <td>332</td>
+    <td>329</td>
+    <td>336</td>
+    <td>330</td>
+    <td>0,30085</td>
+    <td>0,27235</td>
+    <td>0,26565</td>
+    <td>0,27535</td>
+    <td>106,25</td>
+    <td>103,25</td>
+    <td>110,25</td>
+    <td>104,25</td>
+    <td>0,06</td>
+    <td>0,06</td>
+    <td>0,07</td>
+    <td>0,06</td>
+    <td>0,06</td>
+    <td>0,00</td>
+    <td>0,06</td>
+    <td>1,068</td>
+  </tr>
+</tbody>
+</table>
+}}|}}
+            <h2>2. Discussion</h2>
+            The protocol was easy to follow and the constructs were nicely expressed which allowed reliable and comparable measurements. In the process of obtaining transformed DH5&#945; cells we did not encounter any problems with transformation as bacteria showed exceptional growth. The specific ratiometric conversion factor of our plate reader could be determined without further adjustments using the LUDOX-HS40 solution. When measuring the Fluorescein standard curve on a logarithmic scale the data points showed a linear distribution. The final step, the measurement of the fluorescence emission and optical density of the cells, resulted in the raw data shown in table 1.5. To make this data visually accessible the mean values for OD and fluorescence were calculated and plotted against their respective time points which are seen in the following graphs.
+        }}
+    }}
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+            {{Heidelberg/templateus/Imagesection|https://static.igem.org/mediawiki/2017/e/eb/T--Heidelberg--ODGraph.png|Figure 1.3:| Calculated means from both replicates for the OD<sub>600</sub> after each time point|}}
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+            {{Heidelberg/templateus/Imagesection|https://static.igem.org/mediawiki/2017/0/07/T--Heidelberg--FluorescenceGraph.png|Figure 1.4:| Calculated means from both replicates for the fluorescence after each time point|}}
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-                                                                <h1>1. Interlab Study</h1>
-                                                                 <hr>
-<p>As a first step to contribute to this year's iGEM competition we decided to participate in iGEM's fourth International Interlaboratory Study along with many other teams from around the world. This study is organized by iGEM's measurement committee in an effort to establish standardized, reliable and repeatable measurement tools for the iGEM community and the synthetic biology community as a whole. This year's iGEM InterLab study is about establishing a standardized protocol for the measurement of GFP using a plate reader. To start things off we needed a plate reader that is qualified to measure GFP fluorescence. Namely, Tecan Infinite M200 Pro plate reader. Additionally, we needed competent <i>E.coli</i> DH5&alpha;. These were prepared from glycerol stocks. Together with the material iGEM had provided we were ready for work. Throughout our experiments, we tested 8 plasmids (2 controls and 6 test devices) by measuring the OD<sub>600</sub> and the fluorescence of the cells carrying the constructs.
-The workflow can be separated into four segments. The first segment is the transformation of all plasmids into competent  DH5&alpha; cells.
-                                                            <h2>1.1 Transformation of 8 test devices into competent DH5&alpha; cells</h2>
-<p>The transformation was successful for all the plasmids and resulted in a sufficient amount of colonies on all plates. Therefore, two colonies from each plate were picked and inoculated in LB medium containing chloramphenicol over night for approximately 16-18 hours at 37°C and 220 rpm.</p>
-                                                         <h2>1.2 Measurement of LUDOX-HS40 OD600 Reference Point</h2>
-<p>The second segment is the measurement of the LUDOX-HS40 solution to obtain a ratiometric conversion factor which allows us to transform the absorbance data into a standard OD<sub>600</sub> measurement. This is necessary because plate readers in general do not have a 1 cm path length which means that measurements are volume dependent. By doing so, we obtained following data.</p>
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-                                                                  <a href="#" target="_blank" ><img src="https://static.igem.org/mediawiki/2017/4/48/T--Heidelberg--LUDOX-HS40.PNG"  alt="#"></a>
-                                                                    <p>Figure 1.1: Measurement of the LUDOX-HS40 and water in four replicates and calculation of the ratiometric conversion factor</p>
-                                                                </div>
-                                                                </div>
-                                                                </div>
-                                                            <p>
-                                                                With these replicates measured, we obtained a ratiometric conversion factor of 4.30 for our specific plate reader when measured 100 μL of the provided LUDOX-HS40 to water.</p>
-                                                            <h2>1.3 Graphing a fluorescein fluorescence standard curve</h2>
-                                                            <p>
-                                                            In the third segment we were instructed to measure a standard curve for fluorescence of fluorescein which can be used to correct our cell based readings to an
-                                                            equivalent fluorescein concentration. Following the instructions, we could obtain following results for the serial dilution of fluorescein in phosphate buffered
-                                                            saline.
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-                                                                  <a href="#" target="_blank" ><img src="https://static.igem.org/mediawiki/2017/a/a0/T--Heidelberg--FluoresceinSerialDilution.PNG"  alt="#"></a>
-                                                                  <div class="caption">
-                                                                    <p>Figure 1.2: Measurement of a standard curve with four replicates by serial dilution of fluorescein in phosphate buffered saline</p>
-                                                                  </div>
-                                                                </div>
-                                                                </div>
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-                                                                  <a href="#" target="_blank" ><img src="https://static.igem.org/mediawiki/2017/5/57/T--Heidelberg--FluoresceinStandardCurve.png"  alt="#"></a>
-                                                                  <div class="caption">
-                                                                    <p>Figure 1.3: Fluorescence is plotted against the fluorescein concentration</p>
-                                                                  </div>
-                                                                </div>
-                                                                </div>
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-                                                                  <a href="#" target="_blank" ><img src="https://static.igem.org/mediawiki/2017/f/fa/T--Heidelberg--FluoresceinStandardCurveLogScale.png"  alt="#"></a>
-                                                                  <div class="caption">
-                                                                    <p>Figure 1.4: Fluorescence is plotted against the fluorescein concentration on a logarithmic scale</p>
-                                                                  </div>
-                                                                </div>
-                                                                </div>
-                                                                </div>
-                                                            <p>
-                                                            The data give us a more or less linear regression line when plotted in a logarithmic scale but behave more like a quadratic equation when plotted normally.                                                                  </p>
-                                                            <h2>1.4 Measurement of OD600 and Fluorescence of Transformed Cells</h2>
-                                                            <p>
-                                                            In the last of the four segments the OD600 and the fluorescence of the transformed cells were measured according to the protocol after 0, 2, 4 and 6 hours.
-                                                            Measurements of all these time points gave us the following tables and calculations which were conducted with the values we obtained from the standard
-                                                            curves and the reference point.
-                                                            </p>
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-                                                                  <a href="#" target="_blank" ><img src="C:\\Users\\Wolle\\Documents\\iGEM\\Interlab\\Calculations3.png"  alt="#"></a>
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-                                                                    <p>Figure 1.5: Raw data</p>
-                                                                  </div>
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-                                                                  <a href="#" target="_blank" ><img src="https://2017.igem.org/File:RawPlateReadings.PNG"  alt="#"></a>
-                                                                  <div class="caption">
-                                                                    <p>Figure 1.6: Raw data and measurements for the first two time points</p>
-                                                                  </div>
-                                                                </div>
-                                                                </div>
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-                                                                  <a href="#" target="_blank" ><img src="C:\\Users\\Wolle\\Documents\\iGEM\\Interlab\\Calculations2.png"  alt="#"></a>
-                                                                  <div class="caption">
-                                                                    <p>Figure 1.7: Raw data and measurements for the last two time points</p>
-                                                                  </div>
-                                                                </div>
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-                                                                <h2>References</h2>
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+             Conclusively, these data suggest that the Interlab study was successful and that the provided protocol can be shared in the community for standardized fluorescence measurement with a plate reader.
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