Difference between revisions of "Team:ZJUT-China/Description"

 
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<h1>Description</h1>
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<p> Here is a clear and concise introduction of our project. It only provides the most essential information and will be re-edit later. </p>
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<li> A brief description of our project.</li>
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We designed a new kind of genetically engineered E.coli which has the function of light-induced self-lysis or self-flocculation. To achieve this goal, we found two light controlled switches that can work in E.coli, one self-lysis gene named lysis and one self-flocculation gene named bcsB now. We plan to put one light reaction system and one effect gene into the same E.coli cell so that it can react to red or blue light with certain strength and then begin to self-lyse or self-flocculate. And if time permits, we’ll try to put two switches and two functional genes in the same one E.coli cell and test it to see if it could achieve different functions as the reactions to different colors of light.
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<li>Why do we chose to work on this genetically engineered E.coli at first?</li>
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The solid-liquid separation and cell disruption are two major challenges in pharmaceutical industry. Those expensive equipments and toxic chemicals they need are the most annoying parts for fermentation factories. To solve these, we students came out of an idea that if we can provide some kind of E.coli which can be broken or flocculate in a cheap and controllable condition. After searching the literature, we found that light-induced switch system perfectly meets the qualification. The self-lysis gene and the self-flocculation gene had been found during that time.
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<li>References and sources we used in our research.</li>
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①Jeffrey J. Tabor,Anselm Levskaya,and Christopher A. Voigt. Multichromatic control of gene expression in Escherichia coli.J Mol Biol. 2011 January 14; 405(2): 315–324.
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②Wu H, Wang Y, Wang Y, Cao X, Wu Y, Meng Z, Su Q, Wang Z, Yang S, Xu W, Liu S, Cheng P, Wu J, Khan MR, He L, Ma G.Quantitatively relating gene expression to light intensity via the serial connection of blue light sensor and CRISPRi.ACS Synth Biol. 2014 Dec 19;3(12):979-82. doi: 10.1021/sb500059x.
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③Yoshihiro Ojima, Minh Hong Nguyen, Reiki Yajima, and Masahito Taya.Flocculation of Escherichia coli Cells in Association with Enhanced Production of Outer Membrane Vesicles. Appl Environ Microbiol. 2015 Sep 1;81(17):5900-6. doi: 10.1128/AEM.01011-15. Epub 2015 Jun 19.
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④Laura Grande, Valeria Michelacci, Rosangela Tozzoli, Paola Ranieri, Antonella Maugliani, Alfredo Caprioli, and Stefano Morabito. Whole genome sequence comparison of vtx2-converting phages from Enteroaggregative Haemorrhagic Escherichia coli strains. BMC Genomics. 2014; 15(1): 574.Published online 2014 Jul 8. doi:  10.1186/1471-2164-15-574.
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<h5>Advice on writing your Project Description</h5>
 
  
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We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be consist, accurate and unambiguous in your achievements.
 
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Judges like to read your wiki and know exactly what you have achieved. This is how you should think about these sections; from the point of view of the judge evaluating you at the end of the year.
 
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<h2 class="pagetitle"> <strong>Description</strong></h2>
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<h5>References</h5>
 
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<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.</p>
 
  
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<h5>Inspiration</h5>
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<p>See how other teams have described and presented their projects: </p>
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<h3>Conception</h3>
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<li><a href="https://2016.igem.org/Team:Imperial_College/Description">2016 Imperial College</a></li>
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<li><a href="https://2016.igem.org/Team:Wageningen_UR/Description">2016 Wageningen UR</a></li>
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Nowadays intracellular products take up a great share o the market, and with the increase of people’s need, they will create a growing market value. But the way to <b>extra the target products from inside of the cells </b> has always been a problem. Chemical disruption ways will mix in new impurities which need to be separated later, and physical ways may bring high temperature leading to unstable products so they need chillers. Considering of  this problem, we came up with an idea that if <b>cells could self-lyse under the  control of some convenient conditions provided by environment, for example, the light</b>, because light serves as an excellent trigger to achieve precise control on synthetic systems as it can regulate in wavelength, timing, intensity and location. Based on this thought, the project showed in the video just came out. In particular, we focused on Escherichia coli in the project because it’s common used and easy to operate. The project may fit other bacteria in further study.
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<li><a href="https://2014.igem.org/Team:UC_Davis/Project_Overview"> 2014 UC Davis</a></li>
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<li><a href="https://2014.igem.org/Team:SYSU-Software/Overview">2014 SYSU Software</a></li>
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<h3>References and sources we used in our research.</h3>
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<li> Wu H, Wang Y, Wang Y, Cao X, Wu Y, Meng Z, Su Q, Wang Z, Yang S, Xu W, Liu S, Cheng P, Wu J, Khan MR, He L, Ma G., Quantitatively relating gene expression to light intensity via the serial connection of blue light sensor and CRISPRi. ACS Synth Biol. 2014, 3(12): 979-982 </li>
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<li> Gardner L, Deiters A. Light-controlled synthetic gene circuits. Curr Opin Chem Biol. 2012, 16(3-4):292-299 </li>  
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<li> Camsund D, Lindblad P, Jaramillo A. Genetically engineered light sensors for control of bacterial gene expression. Biotechnol J. 2011, 6(7):826-836 </li>  
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<li> Ohlendorf R, Vidavski RR, Eldar A, Moffat K, Möglich A., From dusk till dawn: one-plasmid systems for light-regulated gene expression. J Mol Biol. 2012, 416(4): 534-542 </li>  
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<li> Sun Q, Kuty GF, Arockiasamy A, Xu M, Young R, Sacchettini JC., Regulation of a muralytic enzyme by dynamic membrane topology. Nat Struct Mol Biol. 2009 Nov;16(11):1192-1194 </li>
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Latest revision as of 23:05, 1 November 2017

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ZJUT-China

IGEM

Description


Conception

Nowadays intracellular products take up a great share o the market, and with the increase of people’s need, they will create a growing market value. But the way to extra the target products from inside of the cells has always been a problem. Chemical disruption ways will mix in new impurities which need to be separated later, and physical ways may bring high temperature leading to unstable products so they need chillers. Considering of this problem, we came up with an idea that if cells could self-lyse under the control of some convenient conditions provided by environment, for example, the light, because light serves as an excellent trigger to achieve precise control on synthetic systems as it can regulate in wavelength, timing, intensity and location. Based on this thought, the project showed in the video just came out. In particular, we focused on Escherichia coli in the project because it’s common used and easy to operate. The project may fit other bacteria in further study.




References and sources we used in our research.

  1. Wu H, Wang Y, Wang Y, Cao X, Wu Y, Meng Z, Su Q, Wang Z, Yang S, Xu W, Liu S, Cheng P, Wu J, Khan MR, He L, Ma G., Quantitatively relating gene expression to light intensity via the serial connection of blue light sensor and CRISPRi. ACS Synth Biol. 2014, 3(12): 979-982
  2. Gardner L, Deiters A. Light-controlled synthetic gene circuits. Curr Opin Chem Biol. 2012, 16(3-4):292-299
  3. Camsund D, Lindblad P, Jaramillo A. Genetically engineered light sensors for control of bacterial gene expression. Biotechnol J. 2011, 6(7):826-836
  4. Ohlendorf R, Vidavski RR, Eldar A, Moffat K, Möglich A., From dusk till dawn: one-plasmid systems for light-regulated gene expression. J Mol Biol. 2012, 416(4): 534-542
  5. Sun Q, Kuty GF, Arockiasamy A, Xu M, Young R, Sacchettini JC., Regulation of a muralytic enzyme by dynamic membrane topology. Nat Struct Mol Biol. 2009 Nov;16(11):1192-1194