Difference between revisions of "Team:ZJUT-China/Results"

 
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<h2 class="pagetitle"> <strong>Results</strong></h2>
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<h1>Results</h1>
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<li> The whole light-controlled lysis system (BBa_K2277666) is constructed successfully and was tested using OD600 testing. </li>
 
<li> The whole light-controlled lysis system (BBa_K2277666) is constructed successfully and was tested using OD600 testing. </li>
 
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<p>The whole system’s constitution is shown in the picture.</p>
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<img src="https://static.igem.org/mediawiki/2017/a/a4/ZJUT-System.png ">
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<p> Cells will self-lyse in the absence of blue light.</p>
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<br>
  
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<h5>You should also describe what your results mean: </h5>
 
  
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<h3>Device</h3>
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<li> Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for. </li>
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<li> Show data, but remember all measurement and characterization data must be on part pages in the Registry. </li>
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<li> Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project. </li>
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<th> Light Source</th> <th> Light Intensity</th>
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<td> When we were making the circuit boards using as the light source, function had a priority.According to the requirements of our cells culture, which is, a fixed wavelength of the blue light irradiation which could start our lysin gene.Therefore, in the process of experimental variables involved, we controlled the wavelength and strength of light source. Then we built bottom fixed wavelength light source in the completely dark black case. In order to control conveniently, we designed a multi paralleled DC circuit which was simple, stable and switchable. Battery powered LED lamps were chosen as the light source so that the device would be easy to carry out. </td> <td> 34lux </td>
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<th> Compositions </th> <th> Parameters </th> <th> Notes </th>
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<td> Bottom: pvc cover</td> <td>ф7.6cmcm*0.5cm</td> <td></td>
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<td>Bottom: thick cardboard</td> <td>ф7.6cm*0.5cm</td>  <td></td>
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<td> Cover </td> <td> ф7.6cm*0.5cm</td> <td></td>
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<td> Tube</td> <td>Cylindrical shell ф7.5cm*0.2cm pvc height 17cm</td>  <td>"Wrapped with tin foil, completely opaque.
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With strong and proper diameter, it can be placed in the shaker to work."</td>
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<td> Internal fixing plate </td> <td>"ф7.5cm*0.5cm,Within the decentralized 4 * ф2.5 hollow hole" </td>  <td>Four test tubes can be central placed tightly, tube bottom keep direct contact with the cardboard.</td>
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<td>Light source plate.</td> <td> ф7.5cm*0.5cm</td>  <td>Light (circuit board) is fixed at the bottom, and the switch is fixed outside.When being used, the test tube should be placed from the top into the device, then cap the tube in order to make sure it’s completely opaque.</td>
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<h5> Project Achievements </h5>
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<p>You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.</p>
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<ul>
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<li>A list of linked bullet points of the successful results during your project</li>
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<li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li>
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<h3>Modeling</h3>
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<p> Based on our experiments data and minimized stimulation device, we created a fermenter model of our light-induced self-lysis system. Our model informed the possible positions for company to input the light source. And it also provide cost budget in fermentation process for reference. This model, presenting below, consists of the ideal figure of fermenter and calculating process of the cost budget. </p>
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<b>More details are in the Modeling Part.</b>
  
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<h5>Inspiration</h5>
 
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<p>See how other teams presented their results.</p>
 
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<ul>
 
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<li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results/Pathway">2014 TU Darmstadt </a></li>
 
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<li><a href="https://2014.igem.org/Team:Imperial/Results">2014 Imperial </a></li>
 
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<li><a href="https://2014.igem.org/Team:Paris_Bettencourt/Results">2014 Paris Bettencourt </a></li>
 
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Latest revision as of 23:15, 1 November 2017

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ZJUT-China

IGEM

Results

Experiments

  1. The functional gene lysin (BBa_K2277000) was tested using OD600 testing&dilution-spread method and works well.
  2. The functional gene bcsB (BBa_K2277110) has been tested and being abandoned.
  3. The reporter gene eGFP (BBa_K2277033) works well as reporter gene.
  4. The new FixK2 promoter (BBa_K2277233) has improved the efficiency of the existing part BBa_K1065303.
  5. The light-controlled part (BBa_K2277555) has been tested using eGFP as the reporter gene and works well.
  6. The whole light-controlled lysis system (BBa_K2277666) is constructed successfully and was tested using OD600 testing.

The whole system’s constitution is shown in the picture.

Cells will self-lyse in the absence of blue light.


Device

Light Source Light Intensity
When we were making the circuit boards using as the light source, function had a priority.According to the requirements of our cells culture, which is, a fixed wavelength of the blue light irradiation which could start our lysin gene.Therefore, in the process of experimental variables involved, we controlled the wavelength and strength of light source. Then we built bottom fixed wavelength light source in the completely dark black case. In order to control conveniently, we designed a multi paralleled DC circuit which was simple, stable and switchable. Battery powered LED lamps were chosen as the light source so that the device would be easy to carry out. 34lux
Compositions Parameters Notes
Bottom: pvc cover ф7.6cmcm*0.5cm
Bottom: thick cardboard ф7.6cm*0.5cm
Cover ф7.6cm*0.5cm
Tube Cylindrical shell ф7.5cm*0.2cm pvc height 17cm "Wrapped with tin foil, completely opaque. With strong and proper diameter, it can be placed in the shaker to work."
Internal fixing plate "ф7.5cm*0.5cm,Within the decentralized 4 * ф2.5 hollow hole" Four test tubes can be central placed tightly, tube bottom keep direct contact with the cardboard.
Light source plate. ф7.5cm*0.5cm Light (circuit board) is fixed at the bottom, and the switch is fixed outside.When being used, the test tube should be placed from the top into the device, then cap the tube in order to make sure it’s completely opaque.

Modeling

Based on our experiments data and minimized stimulation device, we created a fermenter model of our light-induced self-lysis system. Our model informed the possible positions for company to input the light source. And it also provide cost budget in fermentation process for reference. This model, presenting below, consists of the ideal figure of fermenter and calculating process of the cost budget.

More details are in the Modeling Part.