Difference between revisions of "Team:BostonU HW/Demonstrate"

 
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background-image: url("https://static.igem.org/mediawiki/2017/9/94/LARGE_background_MARS.png");
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<div class="header" id="Header_Pic"><img src="https://static.igem.org/mediawiki/2017/9/94/LARGE_background_MARS.png" id="BACKGROUND">
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<img src="https://static.igem.org/mediawiki/2017/0/02/MARSbackground.png" id="BACKGROUND">
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<img src="https://static.igem.org/mediawiki/2017/2/22/MARSLogo2.png" id="MARS">
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<img src="https://static.igem.org/mediawiki/2017/e/e3/MARS_Repository.png" id="TITLE">
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<div class="col-md-9" style="color:#eef1f5; font-size:100px; font-family:Arial,Gadget,sans-serif; margin-top:1%;">
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MARS Repository
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</div>
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</div>
 
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<div class="main main-raised" id = "main_text" style="margin-bottom:5%;">
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<div class="main main-raised" style="margin-top:2%;" id = "main_text">
 
<div class="container">
 
<div class="container">
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<h1 class="text-center"> Explore the MARS Repository</h1>
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<h1>Summary</h1>
 
<div class="text_section col-md-12">
 
<div class="text_section col-md-12">
 
<div class="text">
 
<div class="text">
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The MARS Repository was created with the purpose of designing generalized chips relevant to synthetic biology.
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<br>
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Microfluidic devices are often highly specific, performing entire specialized experiments on a chip. This means that these chips are not useful or relevant to an average member of the synthetic biology community.
−
<br>
+
                                 <br>
−
Microfluidic devices are often highly specific, performing entire specialized experiments on a chip. This means that these chips are not useful or relevant to an average member of the synthetic biology community.  
+
−
                                 <br>  
+
 
                                 <br>
 
                                 <br>
 
With this in mind, we set out to make the MARS repository. This repository would consist of generalized microfluidic chips that could be useful to any synthetic biologist.
 
With this in mind, we set out to make the MARS repository. This repository would consist of generalized microfluidic chips that could be useful to any synthetic biologist.
−
<br>  
+
<br>
 
                                 <br>
 
                                 <br>
−
To determine what protocols synbiologist would find useful on a microfluidic devices, we reached out the the Biological Design Center located at Boston University. This community of synthetic biologists gave us insight as to what what procedures are performed every day in the average synbio lab. We were able to identify eight protocols integral to synthetic biology: Cell Lysis, DNA Digestion, Ligation, Transformation, PCR, Fluorescence Testing, Antibiotic Resistance Testing, and Cell Culturing. These eight protocols, as well as Cell Sorting, make up the nine chips currently located in the MARS repository.
+
To determine what protocols synbiologist would find useful on microfluidic devices, we reached out the the Biological Design Center located at Boston University. This community of synthetic biologists gave us insight as to what procedures are performed every day in the average synbio lab. We were able to identify eight protocols integral to synthetic biology: Cell Lysis, DNA Digestion, Ligation, Transformation, PCR, Fluorescence Testing, Antibiotic Resistance Testing, and Cell Culturing. These eight protocols, as well as Cell Sorting, make up the nine chips currently located in the MARS repository.
 
<br>
 
<br>
 
                                 <br>
 
                                 <br>
−
Each of the nine chips located in the MARS repository are located within one of three subcategories:
+
Each of the nine chips located in the MARS repository are located within one of three subcategories:
 
                                 <br>
 
                                 <br>
 
                                 <br></div>
 
                                 <br></div>
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<div class = "row" style="text-align: center">
 
<img src="https://static.igem.org/mediawiki/2017/4/41/Isomodqua.png" >
 
<img src="https://static.igem.org/mediawiki/2017/4/41/Isomodqua.png" >
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</div>
 
<br>
 
<br>
 
                                 <br><div class ="text">
 
                                 <br><div class ="text">
−
These nine chips, each performing a basic individual protocol, can be combined together to perform more complex synthetic biology experiments.  
+
These nine chips, each performing a basic individual protocol, can be combined together to perform more complex synthetic biology experiments.
−
                                <br>
+
 
                                 <br>
 
                                 <br>
−
                                Each chip is fully documented, providing all the necessary files for replication and usage.
 
−
                                These files include:
 
 
                                 <br>
 
                                 <br>
 +
                                Each chip is fully documented, providing all the necessary files for replication and usage. These files include:
 +
                                </div> <br>
 
<ol>
 
<ol>
−
<li>Design Files</li>
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<b><li>Design Files</li></b>
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<li>Manufacturing Specifications and Instructions</li>
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<b><li>Manufacturing Specifications and Instructions</li></b>
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<li>Usage and Testing protocols</li>
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<b><li>Usage and Testing protocols</li></b>
−
</ol>
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</ol> <br>
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                                <br>
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                              <div class = "text" style="margin-bottom:3%;">
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as well as other useful tools and resources to make them as accessible as possible.
+
As well as other useful tools and resources to make them as accessible as possible. Full chip documentation can be found on this Wiki and will be available on <a href="https://github.com/CIDARLAB/MARS" style="padding-right:0px;">Github</a> and <a href ="https://metafluidics.org/" style="padding-right:0px;">Metafluidics</a> as well.  
 
<br>
 
<br>
 
<br>
 
<br>
−
These chips are currently in the water testing phase, but they provide a framework that can utilized and built upon by future iGEM teams and the synbio community to allow for biological testing and optimization.
+
These chips are currently in the water testing phase, but they provide a framework that can utilized and built upon by future iGEM teams and the synbio community to allow for biological testing and optimization. A good starting point to explore the repository from are the Cell Lysis, Cell Sorting, and Transformation chips!
 
</div>
 
</div>
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</div></div>
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<div class="col-md-4">
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</div>
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<div class="text_section row">
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<div class="main main-raised" style="margin-top:5%" id="Chips" >
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<h3 class="text-center"><u>Isolation</u></h3>
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<div class="container">
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<div class="text">
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<h1 class="text-center"> Explore the MARS Repository</h1>
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Lorem ipsum dolor sit amet, consectetur adipiscing elit. Integer volutpat, mi feugiat blandit feugiat, ante nisl dictum lacus, in egestas ligula nulla eu ex. Quisque vel justo congue, volutpat neque at, placerat nulla. Nam malesuada tellus sed justo pretium, eu vulputate mauris elementum. Donec elementum gravida ipsum id rhoncus.
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<div class = "text" style="text-align: center">Click on each chip's image to be directed to its specific page!</div>
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</div>
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</div>
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<div class="col-md-4">
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<div class="text_section row">
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<h3 class="text-center"><u>Modification</u></h3>
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<div class="text">
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Lorem ipsum dolor sit amet, consectetur adipiscing elit. Integer volutpat, mi feugiat blandit feugiat, ante nisl dictum lacus, in egestas ligula nulla eu ex. Quisque vel justo congue, volutpat neque at, placerat nulla. Nam malesuada tellus sed justo pretium, eu vulputate mauris elementum. Donec elementum gravida ipsum id rhoncus.
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<div class="col-md-4">
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<div class="text_section row">
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<h3 class="text-center"><u>Quantification</u></h3>
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<div class="text">
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Lorem ipsum dolor sit amet, consectetur adipiscing elit. Integer volutpat, mi feugiat blandit feugiat, ante nisl dictum lacus, in egestas ligula nulla eu ex. Quisque vel justo congue, volutpat neque at, placerat nulla. Nam malesuada tellus sed justo pretium, eu vulputate mauris elementum. Donec elementum gravida ipsum id rhoncus.
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</div>
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<div class="container">
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<div class="col-md-12">
 
<div class="col-md-12">
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<div class="row">
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<div class="row" style="text-align: center" >
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<h3><u>Isolation</u></h3>
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<h3><b>Isolation</b></h3>
 
<div class="column col-md-4">
 
<div class="column col-md-4">
 
<a href="https://2017.igem.org/Team:BostonU_HW/Lysis"><img src="https://static.igem.org/mediawiki/2017/0/09/MARS_Matrix_Lysis.png" alt="Picture"></a>
 
<a href="https://2017.igem.org/Team:BostonU_HW/Lysis"><img src="https://static.igem.org/mediawiki/2017/0/09/MARS_Matrix_Lysis.png" alt="Picture"></a>
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</div>
 
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<div class="row" style="text-align: center" >
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<h3><u>Modification</u></h3>
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<h3><b><br>Modification</b></h3>
 
<div class="column col-md-4">
 
<div class="column col-md-4">
 
<a href="https://2017.igem.org/Team:BostonU_HW/Transformation"><img src="https://static.igem.org/mediawiki/2017/5/5b/MARS_Matrix_Trans.png" alt="Picture"></a>
 
<a href="https://2017.igem.org/Team:BostonU_HW/Transformation"><img src="https://static.igem.org/mediawiki/2017/5/5b/MARS_Matrix_Trans.png" alt="Picture"></a>
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<h3><u>Quantification</u></h3>
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<h3><b><br>Quantification</b></h3>
 
<div class="column col-md-4" style="margin-bottom:3%;">
 
<div class="column col-md-4" style="margin-bottom:3%;">
 
<a href="https://2017.igem.org/Team:BostonU_HW/Antibiotic"><img src="https://static.igem.org/mediawiki/2017/c/cc/MARS_Matrix_AR.png" alt="Picture"></a>
 
<a href="https://2017.igem.org/Team:BostonU_HW/Antibiotic"><img src="https://static.igem.org/mediawiki/2017/c/cc/MARS_Matrix_AR.png" alt="Picture"></a>
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<!-- THIS IS FOOTER -->
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<div class="col-md-2" style="color:white; margin-bottom:30px; margin-top:5px;">
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<h3>CONTACT US</h3>
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<div style="text-align:center;">
 +
<a href="mailto:igembuhw@gmail.com">
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<img src="https://static.igem.org/mediawiki/2017/7/74/MARS_WHITEEmail.png" style="height:60px; margin-top:20px;">
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</a>
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<a href="https://www.instagram.com/buigemhardware/?hl=en">
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<img src="https://static.igem.org/mediawiki/2017/9/93/MARS_Final_insta.png" style="height:60px; margin-top:20px;">
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</a>
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<a href="https://twitter.com/igemhwbu">
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<img src="https://static.igem.org/mediawiki/2017/b/b6/MARS_Twitter_White.png" style="height:60px; margin-top:20px;">
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</a>
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</div>
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</div>
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<div class="col-md-10" style="margin-bottom:30px;">
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<img src="https://static.igem.org/mediawiki/2017/0/0e/MARS_SponsorsFinal.png" style="width:100%; margin-top:30px;" usemap="#image-map">
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Latest revision as of 23:21, 1 November 2017

BostonU_HW

Repository
MARS Repository

Summary

Microfluidic devices are often highly specific, performing entire specialized experiments on a chip. This means that these chips are not useful or relevant to an average member of the synthetic biology community.

With this in mind, we set out to make the MARS repository. This repository would consist of generalized microfluidic chips that could be useful to any synthetic biologist.

To determine what protocols synbiologist would find useful on microfluidic devices, we reached out the the Biological Design Center located at Boston University. This community of synthetic biologists gave us insight as to what procedures are performed every day in the average synbio lab. We were able to identify eight protocols integral to synthetic biology: Cell Lysis, DNA Digestion, Ligation, Transformation, PCR, Fluorescence Testing, Antibiotic Resistance Testing, and Cell Culturing. These eight protocols, as well as Cell Sorting, make up the nine chips currently located in the MARS repository.

Each of the nine chips located in the MARS repository are located within one of three subcategories:



These nine chips, each performing a basic individual protocol, can be combined together to perform more complex synthetic biology experiments.

Each chip is fully documented, providing all the necessary files for replication and usage. These files include:

  1. Design Files
    2.
  2. Manufacturing Specifications and Instructions
    3.
  3. Usage and Testing protocols
    4.

As well as other useful tools and resources to make them as accessible as possible. Full chip documentation can be found on this Wiki and will be available on Github and Metafluidics as well.

These chips are currently in the water testing phase, but they provide a framework that can utilized and built upon by future iGEM teams and the synbio community to allow for biological testing and optimization. A good starting point to explore the repository from are the Cell Lysis, Cell Sorting, and Transformation chips!

Explore the MARS Repository

Click on each chip's image to be directed to its specific page!

Isolation

Picture

Cellular Lysis

Picture

DNA Digestion

Picture

Cell Sorting


Modification

Picture

Transformation

Picture

PCR

Picture

Ligation


Quantification

Picture

Antibiotic Resistance

Picture

Fluorescence

Picture

Cell Culturing