Difference between revisions of "Team:BostonU HW/Demonstrate"

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{{BostonU_HW}}
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<meta charset="utf-8" />
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<link rel="apple-touch-icon" sizes="76x76" href="assets/img/apple-icon.png">
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<title>Repository</title>
  
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.Title{
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#page_background{
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background-image: url("https://static.igem.org/mediawiki/2017/9/94/LARGE_background_MARS.png");
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background-size:100%;
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.text{
  
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<h3>★  ALERT! </h3>
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.text_section{
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<div class="column full_size">
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<body>
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<h1>Demonstrate</h1>
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<!-- *************THIS IS WHERE PAGE STARTS************* -->
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<h3>Gold Medal Criterion #4</h3>
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<div class="landing-page">
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<div class="wrapper" id="page_background">
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<div class="header" id="Header_Pic"><img src="https://static.igem.org/mediawiki/2017/9/94/LARGE_background_MARS.png" id="BACKGROUND">
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<div class="container" margin-top:"2%;">
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<div class="col-md-3">
  
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<p>
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<img src="https://static.igem.org/mediawiki/2017/2/22/MARSLogo2.png" width="100%" style="margin-top:-37%;">
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Teams that can show their system working under real world conditions are usually good at impressing the judges in iGEM. To achieve gold medal criterion #4, convince the judges that your project works. There are many ways in which your project working could be demonstrated, so there is more than one way to meet this requirement. This gold medal criterion was introduced in 2016, so check our what 2016 teams did to achieve a their gold medals!
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<div class="col-md-9" style="color:#eef1f5; font-size:100px; font-family:Arial,Gadget,sans-serif; margin-top:1%;">
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MARS Repository
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</div>
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</div>
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</div>
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<div class="main main-raised" style="margin-top:2%;" id = "main_text">
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<div class="container">
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<h1>Summary</h1>
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<div class="text_section col-md-12">
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<div class="text">
  
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<p>
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Microfluidic devices are often highly specific, performing entire specialized experiments on a chip. This means that these chips are not useful or relevant to an average member of the synthetic biology community.
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Please see the <a href="https://2017.igem.org/Judging/Medals">2017 Medals Page</a> for more information.
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                                <br>
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</p>
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                                <br>
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With this in mind, we set out to make the MARS repository. This repository would consist of generalized microfluidic chips that could be useful to any synthetic biologist.
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<br>
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                                <br>
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To determine what protocols synbiologist would find useful on microfluidic devices, we reached out the the Biological Design Center located at Boston University. This community of synthetic biologists gave us insight as to what procedures are performed every day in the average synbio lab. We were able to identify eight protocols integral to synthetic biology: Cell Lysis, DNA Digestion, Ligation, Transformation, PCR, Fluorescence Testing, Antibiotic Resistance Testing, and Cell Culturing. These eight protocols, as well as Cell Sorting, make up the nine chips currently located in the MARS repository.
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<br>
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                                <br>
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Each of the nine chips located in the MARS repository are located within one of three subcategories:
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                                <br>
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                                <br></div>
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<div class = "row" style="text-align: center">
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<img src="https://static.igem.org/mediawiki/2017/4/41/Isomodqua.png" >
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</div>
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<br>
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                                <br><div class ="text">
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These nine chips, each performing a basic individual protocol, can be combined together to perform more complex synthetic biology experiments.
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                                <br>
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                                <br>
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                                Each chip is fully documented, providing all the necessary files for replication and usage. These files include:
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                                </div> <br>
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<ol>
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<b><li>Design Files</li></b>
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<b><li>Manufacturing Specifications and Instructions</li></b>
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<b><li>Usage and Testing protocols</li></b>
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</ol> <br>
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                              <div class = "text" style="margin-bottom:3%;">
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As well as other useful tools and resources to make them as accessible as possible. Full chip documentation can be found on this Wiki and will be available on <a href="https://github.com/CIDARLAB/MARS" style="padding-right:0px;">Github</a> and <a href ="https://metafluidics.org/" style="padding-right:0px;">Metafluidics</a> as well.
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<br>
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<br>
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These chips are currently in the water testing phase, but they provide a framework that can utilized and built upon by future iGEM teams and the synbio community to allow for biological testing and optimization. A good starting point to explore the repository from are the Cell Lysis, Cell Sorting, and Transformation chips!
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</div>
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</div></div>
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</div>
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<div class="main main-raised" style="margin-top:5%" id="Chips" >
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<div class="container">
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<h1 class="text-center"> Explore the MARS Repository</h1>
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<div class = "text" style="text-align: center">Click on each chip's image to be directed to its specific page!</div>
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<div class="col-md-12">
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<div class="row" style="text-align: center" >
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<h3><b>Isolation</b></h3>
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<div class="column col-md-4">
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<a href="https://2017.igem.org/Team:BostonU_HW/Lysis"><img src="https://static.igem.org/mediawiki/2017/0/09/MARS_Matrix_Lysis.png" alt="Picture"></a>
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<h4 class="text-center">Cellular Lysis</h4>
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</div>
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<div class="column col-md-4">
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<a href="https://2017.igem.org/Team:BostonU_HW/Digestion"><img src="https://static.igem.org/mediawiki/2017/c/cd/MARS_Matrix_Digest.png" alt="Picture"></a>
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<h4 class="text-center">DNA Digestion</h4>
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</div>
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<div class="column col-md-4">
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<a href="https://2017.igem.org/Team:BostonU_HW/Isolation"><img src="https://static.igem.org/mediawiki/2017/8/8e/MARS_Matrix_CS.png" alt="Picture"></a>
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<h4 class="text-center">Cell Sorting</h4>
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</div>
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</div>
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<div class="row" style="text-align: center" >
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<h3><b><br>Modification</b></h3>
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<div class="column col-md-4">
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<a href="https://2017.igem.org/Team:BostonU_HW/Transformation"><img src="https://static.igem.org/mediawiki/2017/5/5b/MARS_Matrix_Trans.png" alt="Picture"></a>
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<h4 class="text-center">Transformation</h4>
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</div>
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<div class="column col-md-4">
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<a href="https://2017.igem.org/Team:BostonU_HW/PCR"><img src="https://static.igem.org/mediawiki/2017/6/63/MARS_Matrix_PCR.png" alt="Picture"></a>
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<h4 class="text-center">PCR</h4>
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</div>
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<div class="column col-md-4">
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<a href="https://2017.igem.org/Team:BostonU_HW/Ligation"><img src="https://static.igem.org/mediawiki/2017/5/5e/MARS_Matrix_Ligation.png" alt="Picture"></a>
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<h4 class="text-center">Ligation</h4>
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</div>
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</div>
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<div class="row" style="text-align: center" >
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<h3><b><br>Quantification</b></h3>
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<div class="column col-md-4" style="margin-bottom:3%;">
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<a href="https://2017.igem.org/Team:BostonU_HW/Antibiotic"><img src="https://static.igem.org/mediawiki/2017/c/cc/MARS_Matrix_AR.png" alt="Picture"></a>
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<h4 class="text-center">Antibiotic Resistance</h4>
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</div>
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<div class="column col-md-4">
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<a href="https://2017.igem.org/Team:BostonU_HW/Fluorescence"><img src="https://static.igem.org/mediawiki/2017/d/d6/MARS_Matrix_Flo.png" alt="Picture"></a>
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<h4 class="text-center">Fluorescence</h4>
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</div>
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<div class="column col-md-4">
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<a href="https://2017.igem.org/Team:BostonU_HW/Culturing "><img src="https://static.igem.org/mediawiki/2017/f/fb/MARS_Matrix_CC.png" alt="Picture"></a>
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<h4 class="text-center">Cell Culturing</h4>
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</div>
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</div>
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</div>
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</div>
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</div>
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</div>
  
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<div class="wrapper" style="background:#1c1f1f; margin-top:0px;margin-right:0px !important; margin-left:0px !important;" id="Footer">
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<div class="container" style="text-align:center !important">
  
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<div class="col-md-2" style="color:white; margin-bottom:30px; margin-top:5px;">
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<h3>CONTACT US</h3>
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<div style="text-align:center;">
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<a href="mailto:igembuhw@gmail.com">
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<img src="https://static.igem.org/mediawiki/2017/7/74/MARS_WHITEEmail.png" style="height:60px; margin-top:20px;">
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</a>
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<a href="https://www.instagram.com/buigemhardware/?hl=en">
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<img src="https://static.igem.org/mediawiki/2017/9/93/MARS_Final_insta.png" style="height:60px; margin-top:20px;">
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</a>
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<a href="https://twitter.com/igemhwbu">
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<img src="https://static.igem.org/mediawiki/2017/b/b6/MARS_Twitter_White.png" style="height:60px; margin-top:20px;">
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</a>
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</div>
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</div>
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<div class="col-md-10" style="margin-bottom:30px;">
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<img src="https://static.igem.org/mediawiki/2017/0/0e/MARS_SponsorsFinal.png" style="width:100%; margin-top:30px;" usemap="#image-map">
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</div>
 
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<h4> What should we do for our demonstration?</h4>
 
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<h5> Standard teams </h5>
 
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<p>
 
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If you have built a proof of concept system, you can demonstrate it working under real world conditions. If you have built a biological device that is intended to be a sensor, can you show it detecting whatever it is intended to sense. If it is intended to work in the field, you can show how this might work using a simulated version in the lab, or a simulation of your device in the field.<strong> Please note biological materials must not be taken out of the lab</strong>.
 
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<br>
 
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<h5> Special track teams </h5>
 
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<p>
 
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Special track teams can achieve this medal criterion by bringing their work to the Jamboree and showcasing it in the track event. Art & Design, Measurement, Hardware and Software tracks will all have showcase events at the Giant Jamboree.<strong> Please note biological materials must not be taken out of the lab</strong>.
 
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Latest revision as of 23:21, 1 November 2017

BostonU_HW

Repository
MARS Repository

Summary

Microfluidic devices are often highly specific, performing entire specialized experiments on a chip. This means that these chips are not useful or relevant to an average member of the synthetic biology community.

With this in mind, we set out to make the MARS repository. This repository would consist of generalized microfluidic chips that could be useful to any synthetic biologist.

To determine what protocols synbiologist would find useful on microfluidic devices, we reached out the the Biological Design Center located at Boston University. This community of synthetic biologists gave us insight as to what procedures are performed every day in the average synbio lab. We were able to identify eight protocols integral to synthetic biology: Cell Lysis, DNA Digestion, Ligation, Transformation, PCR, Fluorescence Testing, Antibiotic Resistance Testing, and Cell Culturing. These eight protocols, as well as Cell Sorting, make up the nine chips currently located in the MARS repository.

Each of the nine chips located in the MARS repository are located within one of three subcategories:



These nine chips, each performing a basic individual protocol, can be combined together to perform more complex synthetic biology experiments.

Each chip is fully documented, providing all the necessary files for replication and usage. These files include:

  1. Design Files
    2.
  2. Manufacturing Specifications and Instructions
    3.
  3. Usage and Testing protocols
    4.

As well as other useful tools and resources to make them as accessible as possible. Full chip documentation can be found on this Wiki and will be available on Github and Metafluidics as well.

These chips are currently in the water testing phase, but they provide a framework that can utilized and built upon by future iGEM teams and the synbio community to allow for biological testing and optimization. A good starting point to explore the repository from are the Cell Lysis, Cell Sorting, and Transformation chips!

Explore the MARS Repository

Click on each chip's image to be directed to its specific page!

Isolation

Picture

Cellular Lysis

Picture

DNA Digestion

Picture

Cell Sorting


Modification

Picture

Transformation

Picture

PCR

Picture

Ligation


Quantification

Picture

Antibiotic Resistance

Picture

Fluorescence

Picture

Cell Culturing