Difference between revisions of "Team:Heidelberg/Demonstrate"
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Reprogramming of \(\beta\)-Glucoronidase</div> | Reprogramming of \(\beta\)-Glucoronidase</div> | ||
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| − | </div> | + | We used AiGEM to inform the design of a \(\beta\)-glucoronidase mutant with near-wildtype \(\beta\)-galactosidase activity.</div> |
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| + | Organosilicons | ||
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| + | Demonstration of Carbon-Silicon-Bond formation: | ||
| + | <a href="https://2017.igem.org/Team:Heidelberg/Organosilicons#Results" class="demonstrate-button" style="margin: 0px auto !important; background-color: #f8991d !important; color: white !important;"> | ||
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| + | Organosilicons</div> | ||
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| + | We used an engineered cytochrome to catalyze carbon-silicon bond formation in whole-cell extracts.</div> | ||
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Revision as of 00:33, 2 November 2017
Demonstrate
Our concepts & application
In vivo Evolution
Demonstration of our PREDCEL Toolbox:
Cytochrome engineering
We engineered the product specificity of cytochrome P450 1A2 using PREDCEL.
Protein Interaction
We used PREDCEL to evolve a split T7 polymerase towards improved auto-reassembly.
Optogenetics
We used light to control selection stringency during PREDCEL
In silico Evolution
Demonstration of our AiGEM Software:
Evolution of \(\beta\)-Lactamases
We observed a correlation of the measured \(\beta\)-lactamase activities with the protein label scores from AiGEM
Reprogramming of \(\beta\)-Glucoronidase
We used AiGEM to inform the design of a \(\beta\)-glucoronidase mutant with near-wildtype \(\beta\)-galactosidase activity.
Organosilicons
Demonstration of Carbon-Silicon-Bond formation:
Organosilicons
We used an engineered cytochrome to catalyze carbon-silicon bond formation in whole-cell extracts.
