Difference between revisions of "Team:Heidelberg/Demonstrate"
Lukas Adam (Talk | contribs) |
Lukas Adam (Talk | contribs) |
||
| (8 intermediate revisions by the same user not shown) | |||
| Line 2: | Line 2: | ||
{{Heidelberg/navbar}} | {{Heidelberg/navbar}} | ||
<html> | <html> | ||
| + | |||
<div style="background-color: white !important; padding-bottom: 50px !important; margin: 0 !important;"> | <div style="background-color: white !important; padding-bottom: 50px !important; margin: 0 !important;"> | ||
<div class="page-title" style="background-color: white !important; border-top: 0px solid #222;padding-top: 100px"> | <div class="page-title" style="background-color: white !important; border-top: 0px solid #222;padding-top: 100px"> | ||
| Line 10: | Line 11: | ||
Demonstrate </div> | Demonstrate </div> | ||
<div class="header-subtitle" style="color: #393939 !important;"> | <div class="header-subtitle" style="color: #393939 !important;"> | ||
| − | Our project | + | Our project works</div> |
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
| − | + | <div class="container" style="padding-top: 50px" > | |
<div class="col-lg-12 col-md-12 col-xs-12"> | <div class="col-lg-12 col-md-12 col-xs-12"> | ||
<div class="content" style="padding: 20px; width: 100%; height: auto; border-radius: 10px;border: 2px solid #393939 !important; background-color: white !important;color: #393939 !important;font-size: 70px !important;line-height: 70px !important; font-family: 'Josefin Sans', sans-serif !important; font-weight: 700 !important; text-align: center !important"> | <div class="content" style="padding: 20px; width: 100%; height: auto; border-radius: 10px;border: 2px solid #393939 !important; background-color: white !important;color: #393939 !important;font-size: 70px !important;line-height: 70px !important; font-family: 'Josefin Sans', sans-serif !important; font-weight: 700 !important; text-align: center !important"> | ||
In vivo Evolution | In vivo Evolution | ||
| − | </div> | + | <div class="content" style="padding: 20px; width: 100%; height: auto; color: #393939 !important;font-size: 25px !important;line-height: 25px !important; font-family: 'Open Sans', sans-serif !important; font-weight: 300 !important;" > |
| + | Demonstration of our PREDCEL Toolbox: | ||
| + | <a href="https://2017.igem.org/Team:Heidelberg/Cytochrome_Engineering#Results" class="demonstrate-button" style="margin: 0px auto !important; background-color: #f8991d !important; color: white !important;"> | ||
| + | <div style="font-size: 40px !important; color: #393939 !important; padding-top: 10px;text-align: left !important"><i class="fa fa-arrow-right" aria-hidden="true"></i> | ||
| + | Cytochrome engineering</div> | ||
| + | <div style="font-size: 25px !important; color: #393939 !important; padding-top: 10px;padding-left: 50px"> | ||
| + | We engineered the product specificity of cytochrome P450 1A2 using PREDCEL.</div> | ||
| + | </a> | ||
| + | |||
| + | <a href="https://2017.igem.org/Team:Heidelberg/Protein_Interaction#Results" class="demonstrate-button" style="margin: 0px auto !important; background-color: #f8991d !important; color: white !important;"> | ||
| + | <div style="font-size: 40px !important; color: #393939 !important; padding-top: 10px;text-align: left !important"><i class="fa fa-arrow-right" aria-hidden="true"></i> | ||
| + | Protein Interaction</div> | ||
| + | <div style="font-size: 25px !important; color: #393939 !important; padding-top: 10px;padding-left: 50px"> | ||
| + | We used PREDCEL to evolve a split T7 polymerase towards improved auto-reassembly.</div> | ||
| + | </a> | ||
| + | |||
| + | <a href="https://2017.igem.org/Team:Heidelberg/Optogenetics#Results" class="demonstrate-button" style="margin: 0px auto !important; background-color: #f8991d !important; color: white !important;"> | ||
| + | <div style="font-size: 40px !important; color: #393939 !important; padding-top: 10px;text-align: left !important"><i class="fa fa-arrow-right" aria-hidden="true"></i> | ||
| + | Optogenetics</div> | ||
| + | <div style="font-size: 25px !important; color: #393939 !important; padding-top: 10px;padding-left: 50px"> | ||
| + | We used light to control selection stringency during PREDCEL</div> | ||
| + | </a> | ||
</div> | </div> | ||
| + | </div> | ||
| + | </div> | ||
<div class="col-lg-12 col-md-12 col-xs-12"> | <div class="col-lg-12 col-md-12 col-xs-12"> | ||
| − | <div class="content" style="padding: 20px; width: 100%; height: auto; border-radius: 10px;border: 2px solid # | + | <div class="content" style="padding: 20px; width: 100%; height: auto; border-radius: 10px;border: 2px solid #393939 !important; background-color: #393939 !important;color: white !important;font-size: 70px !important;line-height: 70px !important; font-family: 'Josefin Sans', sans-serif !important; font-weight: 700 !important; text-align: center !important"> |
| − | + | In silico Evolution | |
| + | <div class="content" style="padding: 20px; width: 100%; height: auto; color: white !important;font-size: 25px !important;line-height: 25px !important; font-family: 'Open Sans', sans-serif !important; font-weight: 300 !important;" > | ||
| + | Demonstration of our AiGEM Software: | ||
| + | <a href="https://2017.igem.org/Team:Heidelberg/Validation#lacResults" class="demonstrate-button" style="margin: 0px auto !important; background-color: #f8991d !important; color: white !important;"> | ||
| + | <div style="font-size: 40px !important; color: white !important; padding-top: 10px;text-align: left !important"><i class="fa fa-arrow-right" aria-hidden="true"></i> | ||
| + | Evolution of \(\beta\)-Lactamases</div> | ||
| + | <div style="font-size: 25px !important; color: white !important; padding-top: 10px;padding-left: 50px"> | ||
| + | We observed a correlation of the measured \(\beta\)-lactamase activities with the protein label scores from AiGEM</div> | ||
| + | </a> | ||
| + | <a href="https://2017.igem.org/Team:Heidelberg/Validation#gusgalResults" class="demonstrate-button" style="margin: 0px auto !important; background-color: #f8991d !important; color: white !important;"> | ||
| + | <div style="font-size: 40px !important; color: white !important; padding-top: 10px;text-align: left !important"><i class="fa fa-arrow-right" aria-hidden="true"></i> | ||
| + | Reprogramming of \(\beta\)-Glucoronidase</div> | ||
| + | <div style="font-size: 25px !important; color: white !important; padding-top: 10px;padding-left: 50px"> | ||
| + | We used AiGEM to inform the design of a \(\beta\)-glucoronidase mutant with near-wildtype \(\beta\)-galactosidase activity.</div> | ||
| + | </a> | ||
| + | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
<div class="col-lg-12 col-md-12 col-xs-12"> | <div class="col-lg-12 col-md-12 col-xs-12"> | ||
| − | <div class="content" style="padding: 20px; width: 100%; height: auto; border-radius: 10px;border: 2px solid # | + | <div class="content" style="padding: 20px; width: 100%; height: auto; border-radius: 10px;border: 2px solid #f8991d !important; background-color: #f8991d !important;color: white !important;font-size: 70px !important;line-height: 70px !important; font-family: 'Josefin Sans', sans-serif !important; font-weight: 700 !important; text-align: center !important"> |
| − | + | Organosilicons | |
| + | <div class="content" style="padding: 20px; width: 100%; height: auto; color: white !important;font-size: 25px !important;line-height: 25px !important; font-family: 'Open Sans', sans-serif !important; font-weight: 300 !important;" > | ||
| + | Demonstration of Carbon-Silicon-Bond formation: | ||
| + | <a href="https://2017.igem.org/Team:Heidelberg/Organosilicons#Results" class="demonstrate-button" style="margin: 0px auto !important; background-color: #f8991d !important; color: white !important;"> | ||
| + | <div style="font-size: 40px !important; color: white !important; padding-top: 10px;text-align: left !important"><i class="fa fa-arrow-right" aria-hidden="true"></i> | ||
| + | Organosilicons</div> | ||
| + | <div style="font-size: 25px !important; color: white !important; padding-top: 10px;padding-left: 50px"> | ||
| + | We used an engineered cytochrome to catalyze carbon-silicon bond formation in whole-cell extracts.</div> | ||
| + | </a> | ||
| + | </div> | ||
</div> | </div> | ||
| + | |||
</div> | </div> | ||
| + | |||
Latest revision as of 00:35, 2 November 2017
Demonstrate
Our project works
In vivo Evolution
Demonstration of our PREDCEL Toolbox:
Cytochrome engineering
We engineered the product specificity of cytochrome P450 1A2 using PREDCEL.
Protein Interaction
We used PREDCEL to evolve a split T7 polymerase towards improved auto-reassembly.
Optogenetics
We used light to control selection stringency during PREDCEL
In silico Evolution
Demonstration of our AiGEM Software:
Evolution of \(\beta\)-Lactamases
We observed a correlation of the measured \(\beta\)-lactamase activities with the protein label scores from AiGEM
Reprogramming of \(\beta\)-Glucoronidase
We used AiGEM to inform the design of a \(\beta\)-glucoronidase mutant with near-wildtype \(\beta\)-galactosidase activity.
Organosilicons
Demonstration of Carbon-Silicon-Bond formation:
Organosilicons
We used an engineered cytochrome to catalyze carbon-silicon bond formation in whole-cell extracts.
