Difference between revisions of "Team:Heidelberg/Demonstrate"
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Demonstrate </div> | Demonstrate </div> | ||
<div class="header-subtitle" style="color: #393939 !important;"> | <div class="header-subtitle" style="color: #393939 !important;"> | ||
| − | Our | + | Our project works</div> |
</div> | </div> | ||
</div> | </div> | ||
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Cytochrome engineering</div> | Cytochrome engineering</div> | ||
<div style="font-size: 25px !important; color: #393939 !important; padding-top: 10px;padding-left: 50px"> | <div style="font-size: 25px !important; color: #393939 !important; padding-top: 10px;padding-left: 50px"> | ||
| − | + | We engineered the product specificity of cytochrome P450 1A2 using PREDCEL.</div> | |
</a> | </a> | ||
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Protein Interaction</div> | Protein Interaction</div> | ||
<div style="font-size: 25px !important; color: #393939 !important; padding-top: 10px;padding-left: 50px"> | <div style="font-size: 25px !important; color: #393939 !important; padding-top: 10px;padding-left: 50px"> | ||
| − | + | We used PREDCEL to evolve a split T7 polymerase towards improved auto-reassembly.</div> | |
</a> | </a> | ||
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Optogenetics</div> | Optogenetics</div> | ||
<div style="font-size: 25px !important; color: #393939 !important; padding-top: 10px;padding-left: 50px"> | <div style="font-size: 25px !important; color: #393939 !important; padding-top: 10px;padding-left: 50px"> | ||
| − | + | We used light to control selection stringency during PREDCEL</div> | |
</a> | </a> | ||
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
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<div class="col-lg-12 col-md-12 col-xs-12"> | <div class="col-lg-12 col-md-12 col-xs-12"> | ||
<div class="content" style="padding: 20px; width: 100%; height: auto; border-radius: 10px;border: 2px solid #393939 !important; background-color: #393939 !important;color: white !important;font-size: 70px !important;line-height: 70px !important; font-family: 'Josefin Sans', sans-serif !important; font-weight: 700 !important; text-align: center !important"> | <div class="content" style="padding: 20px; width: 100%; height: auto; border-radius: 10px;border: 2px solid #393939 !important; background-color: #393939 !important;color: white !important;font-size: 70px !important;line-height: 70px !important; font-family: 'Josefin Sans', sans-serif !important; font-weight: 700 !important; text-align: center !important"> | ||
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Evolution of \(\beta\)-Lactamases</div> | Evolution of \(\beta\)-Lactamases</div> | ||
<div style="font-size: 25px !important; color: white !important; padding-top: 10px;padding-left: 50px"> | <div style="font-size: 25px !important; color: white !important; padding-top: 10px;padding-left: 50px"> | ||
| − | + | We observed a correlation of the measured \(\beta\)-lactamase activities with the protein label scores from AiGEM</div> | |
</a> | </a> | ||
<a href="https://2017.igem.org/Team:Heidelberg/Validation#gusgalResults" class="demonstrate-button" style="margin: 0px auto !important; background-color: #f8991d !important; color: white !important;"> | <a href="https://2017.igem.org/Team:Heidelberg/Validation#gusgalResults" class="demonstrate-button" style="margin: 0px auto !important; background-color: #f8991d !important; color: white !important;"> | ||
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Reprogramming of \(\beta\)-Glucoronidase</div> | Reprogramming of \(\beta\)-Glucoronidase</div> | ||
<div style="font-size: 25px !important; color: white !important; padding-top: 10px;padding-left: 50px"> | <div style="font-size: 25px !important; color: white !important; padding-top: 10px;padding-left: 50px"> | ||
| − | + | We used AiGEM to inform the design of a \(\beta\)-glucoronidase mutant with near-wildtype \(\beta\)-galactosidase activity.</div> | |
</a> | </a> | ||
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
| + | <div class="col-lg-12 col-md-12 col-xs-12"> | ||
| + | <div class="content" style="padding: 20px; width: 100%; height: auto; border-radius: 10px;border: 2px solid #f8991d !important; background-color: #f8991d !important;color: white !important;font-size: 70px !important;line-height: 70px !important; font-family: 'Josefin Sans', sans-serif !important; font-weight: 700 !important; text-align: center !important"> | ||
| + | Organosilicons | ||
| + | <div class="content" style="padding: 20px; width: 100%; height: auto; color: white !important;font-size: 25px !important;line-height: 25px !important; font-family: 'Open Sans', sans-serif !important; font-weight: 300 !important;" > | ||
| + | Demonstration of Carbon-Silicon-Bond formation: | ||
| + | <a href="https://2017.igem.org/Team:Heidelberg/Organosilicons#Results" class="demonstrate-button" style="margin: 0px auto !important; background-color: #f8991d !important; color: white !important;"> | ||
| + | <div style="font-size: 40px !important; color: white !important; padding-top: 10px;text-align: left !important"><i class="fa fa-arrow-right" aria-hidden="true"></i> | ||
| + | Organosilicons</div> | ||
| + | <div style="font-size: 25px !important; color: white !important; padding-top: 10px;padding-left: 50px"> | ||
| + | We used an engineered cytochrome to catalyze carbon-silicon bond formation in whole-cell extracts.</div> | ||
| + | </a> | ||
| + | </div> | ||
| + | </div> | ||
| + | |||
| + | </div> | ||
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Latest revision as of 00:35, 2 November 2017
Demonstrate
Our project works
In vivo Evolution
Demonstration of our PREDCEL Toolbox:
Cytochrome engineering
We engineered the product specificity of cytochrome P450 1A2 using PREDCEL.
Protein Interaction
We used PREDCEL to evolve a split T7 polymerase towards improved auto-reassembly.
Optogenetics
We used light to control selection stringency during PREDCEL
In silico Evolution
Demonstration of our AiGEM Software:
Evolution of \(\beta\)-Lactamases
We observed a correlation of the measured \(\beta\)-lactamase activities with the protein label scores from AiGEM
Reprogramming of \(\beta\)-Glucoronidase
We used AiGEM to inform the design of a \(\beta\)-glucoronidase mutant with near-wildtype \(\beta\)-galactosidase activity.
Organosilicons
Demonstration of Carbon-Silicon-Bond formation:
Organosilicons
We used an engineered cytochrome to catalyze carbon-silicon bond formation in whole-cell extracts.
