Difference between revisions of "Team:Heidelberg/Materials"
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| + | {{Heidelberg/accord|PACE|{{Heidelberg/templateus/Tablebox||{{#tag:html|<table class="table table-bordered mdl-shadow--4dp" XSSCleaned="overflow-x: scroll !important"> | ||
| + | <thead style="background-color: #005493 !important;"> <tr> | ||
| + | <th>Name</th> <th>Supplier</th> <th></th> </tr> | ||
| + | </thead><tbody> <tr> | ||
| + | <td>Flaschen-Mehrfachverteiler, aus PP</td> <td>Roth </td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Schraubkupplung, PBT, flexibel und frei drehbar, GL 14</td> <td>Roth </td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Schraubkupplung, PBT, flexibel und frei drehbar, GL 18</td> <td>Roth </td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Rotilabo-FEP-Schlauch, transparent, 5 m</td> <td>Roth </td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Rotilabo-FEP-Schlauch, transparent, 10 m</td> <td>Roth </td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Schraubkappe mit Bohrung, PBT, GL 18</td> <td>Roth </td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Schraubkappen mit Bohrung, PBT, GL 14</td> <td>Roth </td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Septen, aus Silikonkautschuk, GL 18</td> <td>Roth </td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Septen, aus Silikonkautschuk, GL 14</td> <td>Roth </td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Laborverschraubungen für Schläuche, Durchmesser 3,2 mm</td> <td>Roth </td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Druckausgleichset für GL 18</td> <td>Roth </td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Druckausgleichssatz für GL 14</td> <td>Roth </td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Ersatz-Schraubverschlusskappen für GL 18</td> <td>Roth </td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Ersatz-Schraubverschlusskappen für GL 14</td> <td>Roth </td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Laborverschraub. für 2- und 3-fach Verteiler, Durchmesser 1,6 mm</td> <td>Roth </td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Laborverschraub. für 2- und 3-fach Verteiler, Durchmesser 3,0 mm</td> <td>Roth </td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Rührreaktor-Set GLS 80 2000 ml</td> <td>Roth </td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Ballonflasche LDPE, Enghals, 10 L</td> <td>Roth</td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Doppelschlaucholiven-Montages, Durchmesser 13 mm</td> <td>Roth</td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Doppelschlaucholiven-Montages, Durchmesser 6 mm</td> <td>Roth</td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Ballonflasche PP, Enghals, 20 L</td> <td>Roth</td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Bottle-Top-Filter mit PES-Membran, 0,2 µm</td> <td>Roth</td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Schraubkappe mit Bohrung, PBT, GL 32</td> <td>Roth</td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Kleinmagnetrührer AGE</td> <td>Roth</td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Schraubkappe mit Bohrung, PBT, GL 45</td> <td>Roth</td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Septen, aus Silikonkautschuk, GL 45</td> <td>Roth</td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Rotilabo-Gewindeflaschen, 1000 ml</td> <td>Roth</td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Rotilabo-Gewindeflaschen, 100 ml</td> <td>Roth</td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Masterflex SC0350 BPT-Verlängerungsschlauch (2,06 mm, Innendurchmesser)</td> <td>Cole Parmer </td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Masterflex SC0346 BPT-Verlängerungsschlauch (1,42mm)</td> <td>Cole Parmer </td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Masterflex SC0342 BPT-Verlängerungsschlauch (0,89mm)</td> <td>Cole Parmer </td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Cole-Parmer Luer-Innenkegel 3/32''-Schlauchverbinder </td> <td>Cole Parmer </td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Cole-Parmer Luer-Innenkegel 1/16''-Schlauchverbinder </td> <td>Cole Parmer </td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Cole-Parmer Luer-Außenkegel mit Lock-Ring 3/32''-Schlauchverbinder</td> <td>Cole Parmer </td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Cole-Parmer Luer-Außenkegel mit Lock Ring 1/16''-Schlauchverbinder</td> <td>Cole Parmer </td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Masterflex Microbore Schlauchsatz zwei Stopper (0,89mm)</td> <td>Cole Parmer </td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Masterflex Microbore Schlauchsatz zwei Stopper (2,06mm)</td> <td>Cole Parmer </td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Masterflex L/S 8-channel multichannel pump head for microbore tubing</td> <td>Cole Parmer </td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Masterflex L/S computerkompatibler Digitalantrieb (bis 100 U/min</td> <td>Cole Parmer </td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Cole Palmer ADCF Adapter, Luer-Innenkegel 1/8''-L-Schlauchverbinder</td> <td>Cole Parmer </td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Cole Palmer ADCF Adapter, Luer-Außenkegel 1/8''-L-Schlauchverbinder</td> <td>Cole Parmer </td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Masterflex SC0353 BPT-Verlängerungsschlauch (2,79mm)</td> <td>Cole Parmer </td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Masterflex Tygon Pumpenschlauch zwei Stopper</td> <td>Cole Parmer </td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Masterflex L/S kleine Kassetten für Pumpenköpfe</td> <td>Cole Parmer </td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Masterflex L/S Achtkanal-Kassetten -Pumpenkopf</td> <td>Cole Parmer </td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Masterflex L/S Economy (7 bis 200 U/min)</td> <td>Cole Parmer </td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>3-Wege Quetschventil, Typ 100P3MP24-05S</td> <td>MSS Scientific</td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Tubing Pharmedbpt 1.85 MMID 100 ft SC0349A</td> <td>Cole Parmer</td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>"Y-Connectors PE 3/32"" 10/PK"</td> <td>Cole Parmer</td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>"Y-Connectors PE 1/16"" 10/PK"</td> <td>Cole Parmer</td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Cartridge L/S Small </td> <td>Cole Parmer</td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Luer w/locknut M 1/16 PP 25/PK</td> <td>Cole Parmer</td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Luer w/locknut M 3/32 PP 25/PK</td> <td>Cole Parmer</td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Fittings Luer F 1/16 PP 25/PK</td> <td>Cole Parmer</td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Fittings Luer F 1/16 PP 25/PK</td> <td>Cole Parmer</td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Tubings Silicone 2-Stop 1.42 6/PK</td> <td>Cole Parmer</td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td>Tubings Silicone 2-Stop 2.06 6/PK</td> <td>Cole Parmer</td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td></td> <td></td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td></td> <td></td> <td></td> </tr> | ||
| + | <tr> | ||
| + | <td></td> <td></td> <td></td> </tr> | ||
| + | </tbody></table> | ||
| + | }}| | ||
| + | }}|table_id-100}} | ||
| + | |||
{{Heidelberg/accord|Polymerases|{{Heidelberg/templateus/Tablebox||{{#tag:html|<table class="table table-bordered mdl-shadow--4dp" XSSCleaned="overflow-x: scroll !important"> | {{Heidelberg/accord|Polymerases|{{Heidelberg/templateus/Tablebox||{{#tag:html|<table class="table table-bordered mdl-shadow--4dp" XSSCleaned="overflow-x: scroll !important"> | ||
<thead style="background-color: #005493 !important;"> <tr> | <thead style="background-color: #005493 !important;"> <tr> | ||
Revision as of 00:37, 2 November 2017
Notebook
Materials
Antibiotics
| Name | Supplier | |
|---|---|---|
| Carbenicillin dissodium salt | Carl Roth | |
| Amicillin sodium salt | Carl Roth | |
| Chloramphenicol | Carl Roth | |
| Kanamycin sulfate | Sigma Aldrich | |
| Tetracycline | Sigma Aldrich | |
| Spectinomycine | Sigma Aldrich |
Enzymes
| Name | Supplier | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| BamI | New England BioLabs | |||||||||
| BamHI-HF | New England BioLabs | |||||||||
| BgIII | New England BioLabs | |||||||||
| BsaI-HF | New England BioLabs | |||||||||
| BsmBI | New England BioLabs | |||||||||
| DpnI | New England BioLabs | |||||||||
| EcoRI | New England BioLabs | |||||||||
| EcoRI-HF | New England BioLabs | |||||||||
| Esp3I | Thermo Fisher Scientific | |||||||||
| HindIII-HF | New England BioLabs | |||||||||
| Hinfl, recombinant | New England BioLabs | |||||||||
| HpaII | New England BioLabs | |||||||||
| KpnI-HF | New England BioLabs | |||||||||
| MfeI-HF | New England BioLabs | |||||||||
| MlyI, recombinant | New England BioLabs | |||||||||
| NheI-HF | New England BioLabs | |||||||||
| NotI-HF | New England BioLabs | |||||||||
| PacI | New England BioLabs | |||||||||
| PstI-HF | New England BioLabs | |||||||||
| PvuI-HF | New England BioLabs | |||||||||
| SalI-HF | New England BioLabs | |||||||||
| Sau3AI | New England BioLabs | |||||||||
| SpeI-HF | New England BioLabs | |||||||||
| XbaI | New England BioLabs |
Equipment
| Equipment | Name | Manufacturer | ||
|---|---|---|---|---|
| Table Centrifuge | Microfuge® 18 Centrifuge | Beckman CoulterTM | ||
| Table Centrifuge | Microfuge® 22R Centrifuge | Beckman CoulterTM | ||
| Centrifuge | Allegra X-12R | Beckman CoulterTM | ||
| Shaker | VORTEXGENIE 2 | Scientific Industries, SITM | ||
| Heating plate (magnet stirrer) | MR Hei-Standard | Heidolph | ||
| Heatblock | QBD2 | Grant | ||
| Heatblock | QBD4 | Grant | ||
| Heatblock (shaking function) | Thermomixer comfort | Eppendorf | ||
| PCR-Machine | MyCycler thermo cycler | BioRad | ||
| PCR-Machine | T100 Therma Cycler | BioRad | ||
| PCR-Machine | Mastercycler nexus X2 | Eppendorf | ||
| Gel documentation | Transluminator | Vilber Lourmat | ||
| Scale (fine) | PioneerTM PA114C | OHAUS | ||
| Scale | PioneerTM PA4101C | OHAUS | ||
| Incubator | KBW 400 | Binder | ||
| Incubator/ Shaker | ISF1-X | Kuhner shaker | ||
| Incubator / Shaker | HT Multitron Version 2 | INFORS | ||
| Plate Reader | Infinite M200 | Tecan | ||
| Plate Reader | GloMax® Discover | Promega | ||
| UV/VIS Spectrometer | Ultrospec 3300 pro | Amersham Biosciences | ||
| Microvulume spectrometer | NanoPhotometer NP80 | Implen | ||
| PH-Meter | PH-Meter 765 Calimatic | Knick | ||
| Lyophilizer | Gefriertrocknungsanlage ALPHA 1-2 LD Plus Best. Nr. 101521 | Christ | ||
| Sonificator | Sonoplus Gm 2070 (2002) | Bandelin | ||
| Vacuum Manifold | Qiavac 24 Plus | Qiagen | ||
| HPLC | HPLC system with binary pump, VWD detector and autosampler | Knauer | ||
| Nucleic acid purification system | QIAcube | Qiagen | ||
| PACE related equipment is specified here: |
Kits
| Name | Supplier | ||
|---|---|---|---|
| QIAquick® Gel Extraction Kit | Qiagen | ||
| QIAquick® PCR Purification Kit | Qiagen | ||
| QIAprep® Spin Miniprep Kit | Qiagen | ||
| DNA Clean & Concentrator™-5 | Zymo Research | ||
| ZymoPURE™ Plasmid Miniprep Kit | Zymo Research | ||
| ZymoPURE™ Plasmid Midiprep Kit | Zymo Research | ||
| NEB iGEM Support Kit 2 | New England Biolabs | ||
| NEB Quick Cloning Box-HF | New England Biolabs | ||
| HiScribe T7 Quick High Yield RNA Synthesis Kit | New England Biolabs | ||
| MinION Basic starter pack (1 MinION, 2 Flow cells, 1 Sequencing Kit, 1 Wash kit) | Oxford Nanopore |
Markers
| Name | Supplier | ||
|---|---|---|---|
| Quick-Load 2-Log DNA Ladder (0.1-10.0 kb) | New England BioLabs | ||
| Quick-Load 1 kb DNA Ladder | New England BioLabs | ||
| 100 bp DNA Ladder | New England BioLabs | ||
| Novex® Sharp Pre-stained Protein Standard | Invitrogen | ||
| SERVA Triple Color Protein Standard | SERVA Electrophoresis |
Media
| Media | Composition | |
|---|---|---|
| LB medium (Luria/Miller für d-2,5 kg Best. Nr.: X968.3 | 10 g NaCl, 10 g tryptone/ peptone, 5 g yeast extract in 1 L dH2O | |
| 2xYT medium für die Molekularb-2,5 kg Best. Nr.: X966.3 | 5 g NaCl, 16 g tryptone/ peptone, 10 g yeast extract in 1 L dH2O | |
| SOB medium | 0,5 % yeast extract, 2 % tryptone/peptone, 10 mM NaCl, 2.5 mM KCl, 10 mM MgCl2, 10 mM MgSO4 | |
| SOB medium | SOB supplemented with 20 mM glucose | |
| M9 minimal medium |
Other Enzymes
| Name | Supplier | ||||||
|---|---|---|---|---|---|---|---|
| Gibson Assembly® Master Mix | New England Biolabs | ||||||
| T4 DNA Ligase | New England Biolabs | ||||||
| T7 RNA Polymerase | New England Biolabs | ||||||
| T4 Polynucleotide Kinase (PNK) | New England Biolabs | ||||||
| T4 RNA Ligase 1 (ssRNA Ligase) | New England Biolabs | ||||||
| Alkaline Phosphatase (CIP) | New England Biolabs | ||||||
| Shrimp Alkaline Phosphatase (rSAP) | New England Biolabs |
PACE
| Name | Supplier | |
|---|---|---|
| Flaschen-Mehrfachverteiler, aus PP | Roth | |
| Schraubkupplung, PBT, flexibel und frei drehbar, GL 14 | Roth | |
| Schraubkupplung, PBT, flexibel und frei drehbar, GL 18 | Roth | |
| Rotilabo-FEP-Schlauch, transparent, 5 m | Roth | |
| Rotilabo-FEP-Schlauch, transparent, 10 m | Roth | |
| Schraubkappe mit Bohrung, PBT, GL 18 | Roth | |
| Schraubkappen mit Bohrung, PBT, GL 14 | Roth | |
| Septen, aus Silikonkautschuk, GL 18 | Roth | |
| Septen, aus Silikonkautschuk, GL 14 | Roth | |
| Laborverschraubungen für Schläuche, Durchmesser 3,2 mm | Roth | |
| Druckausgleichset für GL 18 | Roth | |
| Druckausgleichssatz für GL 14 | Roth | |
| Ersatz-Schraubverschlusskappen für GL 18 | Roth | |
| Ersatz-Schraubverschlusskappen für GL 14 | Roth | |
| Laborverschraub. für 2- und 3-fach Verteiler, Durchmesser 1,6 mm | Roth | |
| Laborverschraub. für 2- und 3-fach Verteiler, Durchmesser 3,0 mm | Roth | |
| Rührreaktor-Set GLS 80 2000 ml | Roth | |
| Ballonflasche LDPE, Enghals, 10 L | Roth | |
| Doppelschlaucholiven-Montages, Durchmesser 13 mm | Roth | |
| Doppelschlaucholiven-Montages, Durchmesser 6 mm | Roth | |
| Ballonflasche PP, Enghals, 20 L | Roth | |
| Bottle-Top-Filter mit PES-Membran, 0,2 µm | Roth | |
| Schraubkappe mit Bohrung, PBT, GL 32 | Roth | |
| Kleinmagnetrührer AGE | Roth | |
| Schraubkappe mit Bohrung, PBT, GL 45 | Roth | |
| Septen, aus Silikonkautschuk, GL 45 | Roth | |
| Rotilabo-Gewindeflaschen, 1000 ml | Roth | |
| Rotilabo-Gewindeflaschen, 100 ml | Roth | |
| Masterflex SC0350 BPT-Verlängerungsschlauch (2,06 mm, Innendurchmesser) | Cole Parmer | |
| Masterflex SC0346 BPT-Verlängerungsschlauch (1,42mm) | Cole Parmer | |
| Masterflex SC0342 BPT-Verlängerungsschlauch (0,89mm) | Cole Parmer | |
| Cole-Parmer Luer-Innenkegel 3/32''-Schlauchverbinder | Cole Parmer | |
| Cole-Parmer Luer-Innenkegel 1/16''-Schlauchverbinder | Cole Parmer | |
| Cole-Parmer Luer-Außenkegel mit Lock-Ring 3/32''-Schlauchverbinder | Cole Parmer | |
| Cole-Parmer Luer-Außenkegel mit Lock Ring 1/16''-Schlauchverbinder | Cole Parmer | |
| Masterflex Microbore Schlauchsatz zwei Stopper (0,89mm) | Cole Parmer | |
| Masterflex Microbore Schlauchsatz zwei Stopper (2,06mm) | Cole Parmer | |
| Masterflex L/S 8-channel multichannel pump head for microbore tubing | Cole Parmer | |
| Masterflex L/S computerkompatibler Digitalantrieb (bis 100 U/min | Cole Parmer | |
| Cole Palmer ADCF Adapter, Luer-Innenkegel 1/8''-L-Schlauchverbinder | Cole Parmer | |
| Cole Palmer ADCF Adapter, Luer-Außenkegel 1/8''-L-Schlauchverbinder | Cole Parmer | |
| Masterflex SC0353 BPT-Verlängerungsschlauch (2,79mm) | Cole Parmer | |
| Masterflex Tygon Pumpenschlauch zwei Stopper | Cole Parmer | |
| Masterflex L/S kleine Kassetten für Pumpenköpfe | Cole Parmer | |
| Masterflex L/S Achtkanal-Kassetten -Pumpenkopf | Cole Parmer | |
| Masterflex L/S Economy (7 bis 200 U/min) | Cole Parmer | |
| 3-Wege Quetschventil, Typ 100P3MP24-05S | MSS Scientific | |
| Tubing Pharmedbpt 1.85 MMID 100 ft SC0349A | Cole Parmer | |
| "Y-Connectors PE 3/32"" 10/PK" | Cole Parmer | |
| "Y-Connectors PE 1/16"" 10/PK" | Cole Parmer | |
| Cartridge L/S Small | Cole Parmer | |
| Luer w/locknut M 1/16 PP 25/PK | Cole Parmer | |
| Luer w/locknut M 3/32 PP 25/PK | Cole Parmer | |
| Fittings Luer F 1/16 PP 25/PK | Cole Parmer | |
| Fittings Luer F 1/16 PP 25/PK | Cole Parmer | |
| Tubings Silicone 2-Stop 1.42 6/PK | Cole Parmer | |
| Tubings Silicone 2-Stop 2.06 6/PK | Cole Parmer | |
Polymerases
| Name | Supplier | ||||||
|---|---|---|---|---|---|---|---|
| OneTaq® Quick-Load® 2X Master Mix with Standard Buffer | New England Biolabs | ||||||
| Phusion Flash High-Fidelity PCR Master Mix | Thermo Fisher Scientific | ||||||
| Q5® High-Fidelity DNA Polymerase 2X Master Mix | New England Biolabs |
