Difference between revisions of "Team:Paris Bettencourt/Results"

 
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<h1>Results</h1>
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<p>Here you can describe the results of your project and your future plans. </p>
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<h5>What should this page contain?</h5>
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<li> Clearly and objectively describe the results of your work.</li>
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<li> Future plans for the project. </li>
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<li> Considerations for replicating the experiments. </li>
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<h5>You should also describe what your results mean: </h5>
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<li> Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for. </li>
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<li> Show data, but remember all measurement and characterization data must be on part pages in the Registry. </li>
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<li> Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project. </li>
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<div id=header1 class="header">RESULTS</div>
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<h1>Optogenetic</h1>
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<div class=text2><div class=text2left><h4>Membrane photosensor</h4><ul><li> PHA production under two light AND-gate.</li><li> Characterisation of slow growth in multi-plasmid system. <li> Chacterisation of AND-gate using transmembrane photoreceptors.</li></ul></li></li></div>
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      <div class=text2right><h4>Optics</h4><ul><li> Results confirm the feasibility of achieving a high targeting resolution at significant length within the gel medium.</li></ul></div></div>
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<h1>Biomaterial</h1>
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<div class=text2><div class=text2left><h4>Calcium Carbonate</h4><ul><li> Coral acid-rich proteins(CARP1-CARP4) were implemented into the expression vector and transformed into Escherichia coli BL21.</li><li> Right size proteins were expressed and checked for calcium carbonate precipitation ability.</li></ul><h4>Polysilicate</h4><ul><li> Characterized a biobrick from iGEM Pasteur</li></ul></div>
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      <div class=text2right><h4>PHA</h4><ul><li> Improved the characterization of part BBa_K1149051 using flow cytometry to better quantify P3HB production in E. coli.</li></ul></div></div>
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<h1>RNA organelle</h1>
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<div class=text1><ul><li> RNA organelle was shown to decrease background expression of GFP, giving evidence to our model.</li><li> The RNA organelle was long-living, as it was observed to irreversible and stable in culture 20h after induction.</li><ul></div>
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<h1>Logic Gate & Photo-Caging</h1>
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<div class=text1><ul><li> Successful testing in cell-free system with Dronpa.</li><li> Found evidence that Dronpa "sticks" the repressors to DNA.</li></ul></div>
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<h1>Human Practice</h1>
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<div class=text2><ul><li> Collected feedback from Makerspace and Fablabs to improve our product design.</li><li> Assessed the safety measures of 3D bio-printing through interviews with specialists.</li><li> Built an Escape Room game to teach a general audience about synthetic biology and safety issues</li></ul><div class=text2left></div>
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<h5> Project Achievements </h5>
 
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<p>You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.</p>
 
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<li>A list of linked bullet points of the successful results during your project</li>
 
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<li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li>
 
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<h5>Inspiration</h5>
 
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<p>See how other teams presented their results.</p>
 
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<li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results/Pathway">2014 TU Darmstadt </a></li>
 
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<li><a href="https://2014.igem.org/Team:Imperial/Results">2014 Imperial </a></li>
 
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<li><a href="https://2014.igem.org/Team:Paris_Bettencourt/Results">2014 Paris Bettencourt </a></li>
 
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Latest revision as of 02:18, 2 November 2017

RESULTS

Optogenetic

Membrane photosensor

  • PHA production under two light AND-gate.
  • Characterisation of slow growth in multi-plasmid system.
  • Chacterisation of AND-gate using transmembrane photoreceptors.

Optics

  • Results confirm the feasibility of achieving a high targeting resolution at significant length within the gel medium.

Biomaterial

Calcium Carbonate

  • Coral acid-rich proteins(CARP1-CARP4) were implemented into the expression vector and transformed into Escherichia coli BL21.
  • Right size proteins were expressed and checked for calcium carbonate precipitation ability.

Polysilicate

  • Characterized a biobrick from iGEM Pasteur

PHA

  • Improved the characterization of part BBa_K1149051 using flow cytometry to better quantify P3HB production in E. coli.

RNA organelle

  • RNA organelle was shown to decrease background expression of GFP, giving evidence to our model.
  • The RNA organelle was long-living, as it was observed to irreversible and stable in culture 20h after induction.

    Logic Gate & Photo-Caging

    • Successful testing in cell-free system with Dronpa.
    • Found evidence that Dronpa "sticks" the repressors to DNA.

    Human Practice

    • Collected feedback from Makerspace and Fablabs to improve our product design.
    • Assessed the safety measures of 3D bio-printing through interviews with specialists.
    • Built an Escape Room game to teach a general audience about synthetic biology and safety issues
    Centre for Research and Interdisciplinarity (CRI)
    Faculty of Medicine Cochin Port-Royal, South wing, 2nd floor
    Paris Descartes University
    24, rue du Faubourg Saint Jacques
    75014 Paris, France
    bettencourt.igem2017@gmail.com