Difference between revisions of "Team:William and Mary/Composite Part"

(Prototype team page)
 
 
(9 intermediate revisions by 3 users not shown)
Line 1: Line 1:
−
{{William_and_Mary}}
+
{{Team:William_and_Mary/CSS_RESET}}
 +
{{Team:William_and_Mary/CSS_FIX}}
 +
{{Team:William_and_Mary/SOURCE}}
 +
{{Team:William_and_Mary/HEADER2}}
 +
 
 
<html>
 
<html>
 +
    <head>
  
  
−
<div class="column full_size judges-will-not-evaluate">
+
<style>
−
<h3>★  ALERT! </h3>
+
−
<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
+
−
<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
+
−
</div>
+
−
<div class="clear"></div>
+
  
 +
      body {
 +
        font-family: 'PT Sans', sans-serif;
 +
        font-size: 13px;
 +
      }
  
  
  
−
<div class="column full_size">
+
table, th, td {
−
<h1>Composite Parts</h1>
+
border-collapse: collapse;
 +
}
  
  
−
<p>
+
th, td {
−
A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_I13507">BBa_I13507</a> is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
+
padding: 1px;
−
</p>
+
}
  
−
<p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p>
+
</style>
  
−
<br>
+
</head>
−
<h3>Best Composite Part Special Prize</h3>
+
  
−
<p>New BioBrick devices can be made by combining existing BioBrick Parts. For example, Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on. To be eligible for this award, this part must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a part you wish to nominate your team for this <a href="https://2017.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.
+
<body>
  
−
<br><br>
+
<br></br>
−
<b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
+
<br></br>
−
<br>
+
−
<div class="highlight">
+
−
<h4>Note</h4>
+
−
<p>This page should list all the composite parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page. Remember judges will only look at the first part in the list for the Best Composite Part award, so put your best part first!</p>
+
  
−
</div>
+
<br></br>
 +
 
 +
<center><img src="https://static.igem.org/mediawiki/2017/f/fe/T--William_and_Mary--compositeee.jpeg" width="430px"/></center>
 +
 
 +
 
 +
<div style='padding-top: 50px;'></div>
 +
 
 +
<br></br>
  
 +
<div style='padding-left: 20%;padding-right: 20%;text-indent: 30px;'>We are nominating our pLac mf-Lon (<a href="http://parts.igem.org/Part:BBa_K2333434"style='text-decoration: underline;'>K2333434</a>) construct for best composite part. This part was a cornerstone of our efforts to produce a modular method to alter gene expression speed, enabling us to test a wide variety of protease concentrations with ease. All of our primary characterization was done using this part, and we think that it will prove invaluable to anyone who wants to test a variety of mf-Lon concentrations without having to undergo a large number of cloning steps. As we demonstrated with our <a href="https://2017.igem.org/Team:William_and_Mary/Dynamic_Control"style='text-decoration: underline;'>IFFL</a> circuit, this part can also be used to produce fully functional circuit motifs, and as such could be used as part of a proof of concept or final implementation of other projects. We'd like to note that this part should fulfill our silver medal criteria, for more information please see our  <a href="https://2017.igem.org/Team:William_and_Mary/For_Judges"style='text-decoration: underline;'>for judges</a> page.
 
</div>
 
</div>
 +
 +
 +
<div style='padding-top: 50px;'></div>
 +
<figure style='padding-left: 200px;'>
 +
<img src='https://static.igem.org/mediawiki/2017/3/3e/T--William_and_Mary--circuit6.jpeg' height = "80%" width = "80%"/>
 +
<figcaption><div style='padding-left: 5px;padding-top: 15px; color: #808080; font-size: 14px;'>Figure 1: Schematic of K2333434, mf-Lon is produced in the presense of IPTG. Part is typically used on backbone psb3K3  </div></figcaption>
 +
</figure>
 +
 +
<div style='padding-top: 50px;'></div>
 +
 +
 +
 +
 +
<center>
 +
<div style = 'padding-left: 0px; padding-bottom: 5px;font-size: 25px' ><b>All Composite Parts:</b></div></center>
 +
 +
<div style='background: #808080; margin: 0px 190px 20px 190px; height:1px;></div>
 +
 +
<div style='padding-top: 0px;'></div>
 +
<center>
 +
<table>
 +
<tr>
 +
<th style='background-color: #BEB9C7;column-width: 150px;'><center>Part</center></th>
 +
<th style='background-color: #BEB9C7;column-width: 350px;'><center>Description</center></th>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2333401"> K2333401 </a></td>
 +
<td style='background-color: #ECE7F2;column-width: 400px; font-size: 15px;'>Cloning Ready pdt #3A with Double Terminator</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2333402"> K2333402 </a></td>
 +
<td style='background-color: #DED9E5;column-width: 400px; font-size: 15px;'>Cloning Ready pdt #3B with Double Terminator</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2333403"> K2333403 </a></td>
 +
<td style='background-color: #ECE7F2;column-width: 400px; font-size: 15px;'>Cloning Ready pdt #3C with Double Terminator</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2333404"> K2333404 </a></td>
 +
<td style='background-color: #DED9E5;column-width: 400px; font-size: 15px;'>Cloning Ready pdt #3D with Double Terminator</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2333405"> K2333405 </a></td>
 +
<td style='background-color: #ECE7F2;column-width: 400px; font-size: 15px;'>Cloning Ready pdt #3D with Double Terminator</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2333406"> K2333406 </a></td>
 +
<td style='background-color: #DED9E5;column-width: 400px; font-size: 15px;'>Cloning Ready pdt #3F with Double Terminator</td>
 +
</tr>
 +
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 150px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2333407"> K2333407 </a></td>
 +
<td style='background-color: #DED9E5;column-width: 350px; font-size: 15px;'>UNS J23100 sfGFP pdt #3A</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 150px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2333408"> K2333408 </a></td>
 +
<td style='background-color: #ECE7F2;column-width: 350px; font-size: 15px;'>UNS J23100 sfGFP pdt #3B</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 150px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2333409"> K2333409 </a></td>
 +
<td style='background-color: #DED9E5;column-width: 350px; font-size: 15px;'>UNS J23100 sfGFP pdt #3C</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 150px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2333410"> K2333410 </a></td>
 +
<td style='background-color: #ECE7F2;column-width: 350px; font-size: 15px;'>UNS J23100 sfGFP pdt #3D</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 150px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2333411"> K2333411 </a></td>
 +
<td style='background-color: #DED9E5;column-width: 350px; font-size: 15px;'>UNS J23100 sfGFP pdt #3E</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 150px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2333412"> K2333412 </a></td>
 +
<td style='background-color: #DED9E5;column-width: 350px; font-size: 15px;'>UNS J23100 sfGFP pdt #3F</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2333413"> K2333413 </a></td>
 +
<td style='background-color: #ECE7F2;column-width: 250px; font-size: 15px;'>J23100 mScarlet-I</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2333414"> K2333414 </a></td>
 +
<td style='background-color: #DED9E5;column-width: 250px; font-size: 15px;'>J23100 mScarlet-I pdt #3A</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2333415"> K2333415 </a></td>
 +
<td style='background-color: #ECE7F2;column-width: 250px; font-size: 15px;'>J23100 mScarlet-I pdt #3B</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2333416"> K2333416 </a></td>
 +
<td style='background-color: #DED9E5;column-width: 250px; font-size: 15px;'>J23100 mScarlet-I pdt #3C</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2333417"> K2333417 </a></td>
 +
<td style='background-color: #ECE7F2;column-width: 250px; font-size: 15px;'>J23100 mScarlet-I pdt #3D</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2333418"> K2333418 </a></td>
 +
<td style='background-color: #DED9E5;column-width: 250px; font-size: 15px;'>J23100 mScarlet-I pdt #3E</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2333419"> K2333419 </a></td>
 +
<td style='background-color: #DED9E5;column-width: 250px; font-size: 15px;'>J23100 mScarlet-I pdt #3F</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 150px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2333420"> K2333420 </a></td>
 +
<td style='background-color: #ECE7F2;column-width: 350px; font-size: 15px;'>UNS pTet sfGFP</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 150px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2333421"> K2333421 </a></td>
 +
<td style='background-color: #DED9E5;column-width: 350px; font-size: 15px;'>UNS pTet sfGFP pdt #3A</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 150px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2333422"> K2333422 </a></td>
 +
<td style='background-color: #ECE7F2;column-width: 350px; font-size: 15px;'>UNS pTet sfGFP pdt #3B</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 150px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2333423"> K2333423 </a></td>
 +
<td style='background-color: #DED9E5;column-width: 350px; font-size: 15px;'>UNS pTet sfGFP pdt #3C</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 150px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2333424"> K2333424 </a></td>
 +
<td style='background-color: #ECE7F2;column-width: 350px; font-size: 15px;'>UNS pTet sfGFP pdt #3D</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 150px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2333425"> K2333425 </a></td>
 +
<td style='background-color: #DED9E5;column-width: 350px; font-size: 15px;'>UNS pTet sfGFP pdt #3E</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 150px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2333426"> K2333426 </a></td>
 +
<td style='background-color: #DED9E5;column-width: 350px; font-size: 15px;'>UNS pTet sfGFP pdt #3F</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2333427"> K2333427 </a></td>
 +
<td style='background-color: #ECE7F2;column-width: 250px; font-size: 15px;'>UNS pTet mScarlet-I</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2333428"> K2333428 </a></td>
 +
<td style='background-color: #DED9E5;column-width: 250px; font-size: 15px;'>UNS pTet mScarlet-I pdt #3A</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2333429"> K2333429 </a></td>
 +
<td style='background-color: #ECE7F2;column-width: 250px; font-size: 15px;'>UNS pTet mScarlet-I pdt #3B</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2333430"> K2333430 </a></td>
 +
<td style='background-color: #DED9E5;column-width: 250px; font-size: 15px;'>UNS pTet mScarlet-I pdt #3C</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2333431"> K2333431 </a></td>
 +
<td style='background-color: #ECE7F2;column-width: 250px; font-size: 15px;'>UNS pTet mScarlet-I pdt #3D</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2333432"> K2333432 </a></td>
 +
<td style='background-color: #DED9E5;column-width: 250px; font-size: 15px;'>UNS pTet mScarlet-I pdt #3E</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2333433"> K2333433 </a></td>
 +
<td style='background-color: #DED9E5;column-width: 250px; font-size: 15px;'>UNS pTet mScarlet-I pdt #3F</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2333434"> K2333434 </a></td>
 +
<td style='background-color: #ECE7F2;column-width: 200px; font-size: 15px;'>pLac0-1 mf-Lon</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2333435"> K2333435 </a></td>
 +
<td style='background-color: #DED9E5;column-width: 200px; font-size: 15px;'>pBad mf-Lon</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2333437"> K2333437 </a></td>
 +
<td style='background-color: #DED9E5;column-width: 250px; font-size: 15px;'>Copper Sensor pdt #3A</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2333438"> K2333438 </a></td>
 +
<td style='background-color: #ECE7F2;column-width: 250px; font-size: 15px;'>Copper Sensor pdt #3B</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2333439"> K2333439 </a></td>
 +
<td style='background-color: #DED9E5;column-width: 250px; font-size: 15px;'>Copper Sensor pdt #3C</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2333440"> K2333440 </a></td>
 +
<td style='background-color: #ECE7F2;column-width: 250px; font-size: 15px;'>Copper Sensor pdt #3D</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2333441"> K2333441 </a></td>
 +
<td style='background-color: #DED9E5;column-width: 250px; font-size: 15px;'>Copper Sensor pdt #3E</td>
 +
</tr>
 +
 +
<tr>
 +
<td style='background-color: #9892A1;column-width: 50px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2333442"> K2333442 </a></td>
 +
<td style='background-color: #DED9E5;column-width: 250px; font-size: 15px;'>Copper Sensor pdt #3F</td>
 +
</tr>
 +
 +
 +
</table>
 +
</center>
 +
 +
 +
 +
</body>
  
 
</html>
 
</html>
 +
 +
{{Team:William_and_Mary/JS_SOURCE}}
 +
{{Team:William_and_Mary/FOOTER_SMALL}}

Latest revision as of 02:45, 2 November 2017









We are nominating our pLac mf-Lon (K2333434) construct for best composite part. This part was a cornerstone of our efforts to produce a modular method to alter gene expression speed, enabling us to test a wide variety of protease concentrations with ease. All of our primary characterization was done using this part, and we think that it will prove invaluable to anyone who wants to test a variety of mf-Lon concentrations without having to undergo a large number of cloning steps. As we demonstrated with our IFFL circuit, this part can also be used to produce fully functional circuit motifs, and as such could be used as part of a proof of concept or final implementation of other projects. We'd like to note that this part should fulfill our silver medal criteria, for more information please see our for judges page.
Figure 1: Schematic of K2333434, mf-Lon is produced in the presense of IPTG. Part is typically used on backbone psb3K3
All Composite Parts:
Part
Description
K2333401 Cloning Ready pdt #3A with Double Terminator
K2333402 Cloning Ready pdt #3B with Double Terminator
K2333403 Cloning Ready pdt #3C with Double Terminator
K2333404 Cloning Ready pdt #3D with Double Terminator
K2333405 Cloning Ready pdt #3D with Double Terminator
K2333406 Cloning Ready pdt #3F with Double Terminator
K2333407 UNS J23100 sfGFP pdt #3A
K2333408 UNS J23100 sfGFP pdt #3B
K2333409 UNS J23100 sfGFP pdt #3C
K2333410 UNS J23100 sfGFP pdt #3D
K2333411 UNS J23100 sfGFP pdt #3E
K2333412 UNS J23100 sfGFP pdt #3F
K2333413 J23100 mScarlet-I
K2333414 J23100 mScarlet-I pdt #3A
K2333415 J23100 mScarlet-I pdt #3B
K2333416 J23100 mScarlet-I pdt #3C
K2333417 J23100 mScarlet-I pdt #3D
K2333418 J23100 mScarlet-I pdt #3E
K2333419 J23100 mScarlet-I pdt #3F
K2333420 UNS pTet sfGFP
K2333421 UNS pTet sfGFP pdt #3A
K2333422 UNS pTet sfGFP pdt #3B
K2333423 UNS pTet sfGFP pdt #3C
K2333424 UNS pTet sfGFP pdt #3D
K2333425 UNS pTet sfGFP pdt #3E
K2333426 UNS pTet sfGFP pdt #3F
K2333427 UNS pTet mScarlet-I
K2333428 UNS pTet mScarlet-I pdt #3A
K2333429 UNS pTet mScarlet-I pdt #3B
K2333430 UNS pTet mScarlet-I pdt #3C
K2333431 UNS pTet mScarlet-I pdt #3D
K2333432 UNS pTet mScarlet-I pdt #3E
K2333433 UNS pTet mScarlet-I pdt #3F
K2333434 pLac0-1 mf-Lon
K2333435 pBad mf-Lon
K2333437 Copper Sensor pdt #3A
K2333438 Copper Sensor pdt #3B
K2333439 Copper Sensor pdt #3C
K2333440 Copper Sensor pdt #3D
K2333441 Copper Sensor pdt #3E
K2333442 Copper Sensor pdt #3F