Difference between revisions of "Team:East Chapel Hill/Contribution"

 
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{{East_Chapel_Hill}}
 
 
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<h1>Contribution</h1>
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<h3>Bronze Medal Criterion #4</h3>
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<p><b>Standard Tracks:</b> Participate in the Interlab Measurement Study (to be documented on your InterLab page) and/or improve the characterization of an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2017 part number range. Teams who are working on improving the characterization of an existing part should document their experimental design here, along with an explanation for why they chose that part to improve. Data can also be shown here, but it MUST also be documented on the part's Main Page in the Registry.
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<b>Special Tracks:</b> Document at least one new substantial contribution to the iGEM community that showcases a project related to BioBricks. This contribution should be central to your project and equivalent in difficulty to making and submitting a BioBrick part.
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          <img src="https://static.igem.org/mediawiki/2017/6/65/T--East_Chapel_Hill--Icon-new.png">
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        <li><a href="https://2017.igem.org/Team:East_Chapel_Hill">Home</a></li>
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        <a class="dropdown-toggle" data-toggle="dropdown" href="https://2017.igem.org/Team:East_Chapel_Hill/Team">Team
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          <li><a href="https://2017.igem.org/Team:East_Chapel_Hill/Team">Members</a></li>
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          <li><a href="https://2017.igem.org/Team:East_Chapel_Hill/Attributions">Attributions</a></li>
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        <a class="dropdown-toggle" data-toggle="dropdown" href="https://2017.igem.org/Team:East_Chapel_Hill/Design">Project
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          <li><a href="https://2017.igem.org/Team:East_Chapel_Hill/Design">Design and Method</a></li>
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          <li><a href="https://2017.igem.org/Team:East_Chapel_Hill/Notebook">Lab Notebook</a></li>
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          <li><a href="https://2017.igem.org/Team:East_Chapel_Hill/Composite_Part">Composite Part</a></li>
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          <li><a href="https://2017.igem.org/Team:East_Chapel_Hill/Contribution">Contribution</a></li>
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          <li><a href="https://2017.igem.org/Team:East_Chapel_Hill/Safety">Safety</a></li>
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        <a class="dropdown-toggle" data-toggle="dropdown" href="https://2017.igem.org/Team:East_Chapel_Hill/Results">Results
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          <li><a href="https://2017.igem.org/Team:East_Chapel_Hill/Results">Results and Future Directions</a></li>
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          <li><a href="https://2017.igem.org/Team:East_Chapel_Hill/Demonstrate">Demonstrate</a></li>
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          <li><a href="https://2017.igem.org/Team:East_Chapel_Hill/Measurement">Measurement</a></li>
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        <a class="dropdown-toggle" data-toggle="dropdown" href="https://2017.igem.org/Team:East_Chapel_Hill/HP/Silver">Human Practices
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          <li><a href="https://2017.igem.org/Team:East_Chapel_Hill/HP/Silver">HP Silver</a></li>
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          <li><a href="https://2017.igem.org/Team:East_Chapel_Hill/HP/Gold_Integrated">HP Gold</a></li>
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          <li><a href="https://2017.igem.org/Team:East_Chapel_Hill/Engagement">Engagement</a></li>       
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          <li><a href="https://2017.igem.org/Team:East_Chapel_Hill/Collaborations">Collaborations</a></li>
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<h1>Contribution</h1>
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Our new Part: <a href="http://parts.igem.org/Part:BBa_K2290000"><u>BBa_KK2990000</u></a>
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<b>Plasmid Backbone:</b> <a href="http://parts.igem.org/wiki/index.php?title=Part:pSB1A3"><u>pSB1A3</u></a>
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Below is a list of the basic parts that we used in our part <b><a href="http://parts.igem.org/Part:BBa_K2290000"><u>BBa_KK2990000</u></a> </b>:
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<b><a href="http://parts.igem.org/Part:BBa_J23100"><u>BBa_J23100</u></a></b> – a constitutively active promoter from the Anderson collection.
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<b><a href="http://parts.igem.org/Part:BBa_K2290008"><u>BBa_K2290008</u></a></b> – The <i>B. cereus</i> riboswitch with an engineered terminator that results in less read through/background. (This part is highly similar to BBa_K911003, but the BBa_K911003 sequence includes both the Lys promoter and the riboswitch).
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<b><a href="http://parts.igem.org/Part:BBa_K2290004"><u>BBa_K2290004</u></a></b> – This is a sequence for a strong RBS that doesn't appear to be in the iGEM repository.
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<b><a href="http://parts.igem.org/Part:BBa_K2290005"><u>BBa_K2290005</u></a></b> – This is an <i>E. coli</i> optimized chloramphenicol resistance gene that we had synthesized.
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<b><a href="http://parts.igem.org/Part:BBa_B0010"><u>BBa_B0010</u></a></b> – This is the T1 terminator from <i>E. coli</i> rrnB, reportedly a strong terminator.
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We characterized and improved upon part <b><a href="http://parts.igem.org/Part:BBa_K911003"><u>BBa_K911003</u></a></b>. First, BBa_K911003 listed as a basic part in the iGEM repository is actually a composite part that consists of a promoter and the <i>B. cereus</i> fluoride riboswitch. We more accurately annotated the <i>B. cereus</i> fluoride riboswitch alone as <b><a href="http://parts.igem.org/Part:BBa_K2290008"><u>BBa_K2290008</u></a></b>. We then characterized the responsiveness of the the <i>B. cereus</i> riboswitch and found that the switch works best at 100uM fluoride. To our knowledge part BBa_K911003 was not characterized in iGEM. We also used part <b><a href="http://parts.igem.org/Part:BBa_J23100"><u>BBa_J23100</u></a></b> from the Anderson promoter collection to regulate the expression of our operon and the <b><a href="http://parts.igem.org/Part:BBa_B0010"><u>BBa_B0010</u></a></b> terminator to efficiently terminate transcription . Given, that our composite part works these other two parts are functioning as intended in our operon.
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Latest revision as of 02:53, 2 November 2017

Contribution

Our new Part: BBa_KK2990000

Plasmid Backbone: pSB1A3

Below is a list of the basic parts that we used in our part BBa_KK2990000 :

BBa_J23100 – a constitutively active promoter from the Anderson collection.

BBa_K2290008 – The B. cereus riboswitch with an engineered terminator that results in less read through/background. (This part is highly similar to BBa_K911003, but the BBa_K911003 sequence includes both the Lys promoter and the riboswitch).

BBa_K2290004 – This is a sequence for a strong RBS that doesn't appear to be in the iGEM repository.

BBa_K2290005 – This is an E. coli optimized chloramphenicol resistance gene that we had synthesized.

BBa_B0010 – This is the T1 terminator from E. coli rrnB, reportedly a strong terminator.

We characterized and improved upon part BBa_K911003. First, BBa_K911003 listed as a basic part in the iGEM repository is actually a composite part that consists of a promoter and the B. cereus fluoride riboswitch. We more accurately annotated the B. cereus fluoride riboswitch alone as BBa_K2290008. We then characterized the responsiveness of the the B. cereus riboswitch and found that the switch works best at 100uM fluoride. To our knowledge part BBa_K911003 was not characterized in iGEM. We also used part BBa_J23100 from the Anderson promoter collection to regulate the expression of our operon and the BBa_B0010 terminator to efficiently terminate transcription . Given, that our composite part works these other two parts are functioning as intended in our operon.