Difference between revisions of "Team:East Chapel Hill/Contribution"
Cecilialee (Talk | contribs) |
|||
| (12 intermediate revisions by 2 users not shown) | |||
| Line 105: | Line 105: | ||
<li><a href="https://2017.igem.org/Team:East_Chapel_Hill/HP/Silver">HP Silver</a></li> | <li><a href="https://2017.igem.org/Team:East_Chapel_Hill/HP/Silver">HP Silver</a></li> | ||
<li><a href="https://2017.igem.org/Team:East_Chapel_Hill/HP/Gold_Integrated">HP Gold</a></li> | <li><a href="https://2017.igem.org/Team:East_Chapel_Hill/HP/Gold_Integrated">HP Gold</a></li> | ||
| − | <li><a href="https://2017.igem.org/Team:East_Chapel_Hill/Engagement">Engagement</a></li> | + | <li><a href="https://2017.igem.org/Team:East_Chapel_Hill/Engagement">Engagement</a></li> |
| + | <li><a href="https://2017.igem.org/Team:East_Chapel_Hill/Collaborations">Collaborations</a></li> | ||
</ul> | </ul> | ||
</li> | </li> | ||
| − | |||
| − | |||
</ul> | </ul> | ||
</div> | </div> | ||
| − | </nav> | + | </nav> |
<div class="container" style="color:#feffff; margin-top:50px;"> | <div class="container" style="color:#feffff; margin-top:50px;"> | ||
| − | <div class="row | + | <div class="row"> |
| − | |||
<h1>Contribution</h1> | <h1>Contribution</h1> | ||
<p style="font-size:18px;"> | <p style="font-size:18px;"> | ||
| − | Part: BBa_K2290000 </ | + | Our new Part: <a href="http://parts.igem.org/Part:BBa_K2290000"><u>BBa_KK2990000</u></a> |
| − | < | + | <br><br> |
| − | < | + | <b>Plasmid Backbone:</b> <a href="http://parts.igem.org/wiki/index.php?title=Part:pSB1A3"><u>pSB1A3</u></a> |
| − | Below is a list of the basic parts that we used in our <b> | + | <br><br> |
| − | < | + | Below is a list of the basic parts that we used in our part <b><a href="http://parts.igem.org/Part:BBa_K2290000"><u>BBa_KK2990000</u></a> </b>: |
| − | < | + | <br><br> |
| − | < | + | <b><a href="http://parts.igem.org/Part:BBa_J23100"><u>BBa_J23100</u></a></b> – a constitutively active promoter from the Anderson collection. |
| − | < | + | <br><br> |
| − | < | + | <b><a href="http://parts.igem.org/Part:BBa_K2290008"><u>BBa_K2290008</u></a></b> – The <i>B. cereus</i> riboswitch with an engineered terminator that results in less read through/background. (This part is highly similar to BBa_K911003, but the BBa_K911003 sequence includes both the Lys promoter and the riboswitch). |
| − | < | + | <br><br> |
| + | <b><a href="http://parts.igem.org/Part:BBa_K2290004"><u>BBa_K2290004</u></a></b> – This is a sequence for a strong RBS that doesn't appear to be in the iGEM repository. | ||
| + | <br><br> | ||
| + | <b><a href="http://parts.igem.org/Part:BBa_K2290005"><u>BBa_K2290005</u></a></b> – This is an <i>E. coli</i> optimized chloramphenicol resistance gene that we had synthesized. | ||
| + | <br><br> | ||
| + | <b><a href="http://parts.igem.org/Part:BBa_B0010"><u>BBa_B0010</u></a></b> – This is the T1 terminator from <i>E. coli</i> rrnB, reportedly a strong terminator. | ||
| + | <br><br> | ||
| + | We characterized and improved upon part <b><a href="http://parts.igem.org/Part:BBa_K911003"><u>BBa_K911003</u></a></b>. First, BBa_K911003 listed as a basic part in the iGEM repository is actually a composite part that consists of a promoter and the <i>B. cereus</i> fluoride riboswitch. We more accurately annotated the <i>B. cereus</i> fluoride riboswitch alone as <b><a href="http://parts.igem.org/Part:BBa_K2290008"><u>BBa_K2290008</u></a></b>. We then characterized the responsiveness of the the <i>B. cereus</i> riboswitch and found that the switch works best at 100uM fluoride. To our knowledge part BBa_K911003 was not characterized in iGEM. We also used part <b><a href="http://parts.igem.org/Part:BBa_J23100"><u>BBa_J23100</u></a></b> from the Anderson promoter collection to regulate the expression of our operon and the <b><a href="http://parts.igem.org/Part:BBa_B0010"><u>BBa_B0010</u></a></b> terminator to efficiently terminate transcription . Given, that our composite part works these other two parts are functioning as intended in our operon. | ||
| + | <br><br> | ||
| + | </p> | ||
| − | </ | + | <center><img src="https://static.igem.org/mediawiki/2017/5/53/T--East_Chapel_Hill--part.png" style="width:70%"></center> |
| + | |||
| + | <br><br> | ||
| + | </div> | ||
| + | </div> | ||
</html> | </html> | ||
Latest revision as of 02:53, 2 November 2017
Contribution
Our new Part: BBa_KK2990000
Plasmid Backbone: pSB1A3
Below is a list of the basic parts that we used in our part BBa_KK2990000 :
BBa_J23100 – a constitutively active promoter from the Anderson collection.
BBa_K2290008 – The B. cereus riboswitch with an engineered terminator that results in less read through/background. (This part is highly similar to BBa_K911003, but the BBa_K911003 sequence includes both the Lys promoter and the riboswitch).
BBa_K2290004 – This is a sequence for a strong RBS that doesn't appear to be in the iGEM repository.
BBa_K2290005 – This is an E. coli optimized chloramphenicol resistance gene that we had synthesized.
BBa_B0010 – This is the T1 terminator from E. coli rrnB, reportedly a strong terminator.
We characterized and improved upon part BBa_K911003. First, BBa_K911003 listed as a basic part in the iGEM repository is actually a composite part that consists of a promoter and the B. cereus fluoride riboswitch. We more accurately annotated the B. cereus fluoride riboswitch alone as BBa_K2290008. We then characterized the responsiveness of the the B. cereus riboswitch and found that the switch works best at 100uM fluoride. To our knowledge part BBa_K911003 was not characterized in iGEM. We also used part BBa_J23100 from the Anderson promoter collection to regulate the expression of our operon and the BBa_B0010 terminator to efficiently terminate transcription . Given, that our composite part works these other two parts are functioning as intended in our operon.
