Difference between revisions of "Team:East Chapel Hill/Contribution"
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<li><a href="https://2017.igem.org/Team:East_Chapel_Hill/HP/Silver">HP Silver</a></li> | <li><a href="https://2017.igem.org/Team:East_Chapel_Hill/HP/Silver">HP Silver</a></li> | ||
<li><a href="https://2017.igem.org/Team:East_Chapel_Hill/HP/Gold_Integrated">HP Gold</a></li> | <li><a href="https://2017.igem.org/Team:East_Chapel_Hill/HP/Gold_Integrated">HP Gold</a></li> | ||
| − | <li><a href="https://2017.igem.org/Team:East_Chapel_Hill/Engagement">Engagement</a></li> | + | <li><a href="https://2017.igem.org/Team:East_Chapel_Hill/Engagement">Engagement</a></li> |
| + | <li><a href="https://2017.igem.org/Team:East_Chapel_Hill/Collaborations">Collaborations</a></li> | ||
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<b><a href="http://parts.igem.org/Part:BBa_B0010"><u>BBa_B0010</u></a></b> – This is the T1 terminator from <i>E. coli</i> rrnB, reportedly a strong terminator. | <b><a href="http://parts.igem.org/Part:BBa_B0010"><u>BBa_B0010</u></a></b> – This is the T1 terminator from <i>E. coli</i> rrnB, reportedly a strong terminator. | ||
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| − | We characterized and improved upon part <b><a href="http://parts.igem.org/Part:BBa_K911003"><u>BBa_K911003</u></a></b>. First, | + | We characterized and improved upon part <b><a href="http://parts.igem.org/Part:BBa_K911003"><u>BBa_K911003</u></a></b>. First, BBa_K911003 listed as a basic part in the iGEM repository is actually a composite part that consists of a promoter and the <i>B. cereus</i> fluoride riboswitch. We more accurately annotated the <i>B. cereus</i> fluoride riboswitch alone as <b><a href="http://parts.igem.org/Part:BBa_K2290008"><u>BBa_K2290008</u></a></b>. We then characterized the responsiveness of the the <i>B. cereus</i> riboswitch and found that the switch works best at 100uM fluoride. To our knowledge part BBa_K911003 was not characterized in iGEM. We also used part <b><a href="http://parts.igem.org/Part:BBa_J23100"><u>BBa_J23100</u></a></b> from the Anderson promoter collection to regulate the expression of our operon and the <b><a href="http://parts.igem.org/Part:BBa_B0010"><u>BBa_B0010</u></a></b> terminator to efficiently terminate transcription . Given, that our composite part works these other two parts are functioning as intended in our operon. |
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Latest revision as of 02:53, 2 November 2017
Contribution
Our new Part: BBa_KK2990000
Plasmid Backbone: pSB1A3
Below is a list of the basic parts that we used in our part BBa_KK2990000 :
BBa_J23100 – a constitutively active promoter from the Anderson collection.
BBa_K2290008 – The B. cereus riboswitch with an engineered terminator that results in less read through/background. (This part is highly similar to BBa_K911003, but the BBa_K911003 sequence includes both the Lys promoter and the riboswitch).
BBa_K2290004 – This is a sequence for a strong RBS that doesn't appear to be in the iGEM repository.
BBa_K2290005 – This is an E. coli optimized chloramphenicol resistance gene that we had synthesized.
BBa_B0010 – This is the T1 terminator from E. coli rrnB, reportedly a strong terminator.
We characterized and improved upon part BBa_K911003. First, BBa_K911003 listed as a basic part in the iGEM repository is actually a composite part that consists of a promoter and the B. cereus fluoride riboswitch. We more accurately annotated the B. cereus fluoride riboswitch alone as BBa_K2290008. We then characterized the responsiveness of the the B. cereus riboswitch and found that the switch works best at 100uM fluoride. To our knowledge part BBa_K911003 was not characterized in iGEM. We also used part BBa_J23100 from the Anderson promoter collection to regulate the expression of our operon and the BBa_B0010 terminator to efficiently terminate transcription . Given, that our composite part works these other two parts are functioning as intended in our operon.
