Difference between revisions of "Team:East Chapel Hill/Demonstrate"

 
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<h1>Demonstrate</h1>
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<h3>Gold Medal Criterion #4</h3>
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Teams that can show their system working under real world conditions are usually good at impressing the judges in iGEM. To achieve gold medal criterion #4, convince the judges that your project works. There are many ways in which your project working could be demonstrated, so there is more than one way to meet this requirement. This gold medal criterion was introduced in 2016, so check our what 2016 teams did to achieve a their gold medals!
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Please see the <a href="https://2017.igem.org/Judging/Medals">2017 Medals Page</a> for more information.
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<h4> What should we do for our demonstration?</h4>
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<h5> Standard teams </h5>
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If you have built a proof of concept system, you can demonstrate it working under real world conditions. If you have built a biological device that is intended to be a sensor, can you show it detecting whatever it is intended to sense. If it is intended to work in the field, you can show how this might work using a simulated version in the lab, or a simulation of your device in the field.<strong> Please note biological materials must not be taken out of the lab</strong>.
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          <img src="https://static.igem.org/mediawiki/2017/6/65/T--East_Chapel_Hill--Icon-new.png">
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        <li><a href="https://2017.igem.org/Team:East_Chapel_Hill">Home</a></li>
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          <li><a href="https://2017.igem.org/Team:East_Chapel_Hill/Team">Members</a></li>
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          <li><a href="https://2017.igem.org/Team:East_Chapel_Hill/Design">Design and Method</a></li>
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          <li><a href="https://2017.igem.org/Team:East_Chapel_Hill/Composite_Part">Composite Part</a></li>
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          <li><a href="https://2017.igem.org/Team:East_Chapel_Hill/Safety">Safety</a></li>
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        <a class="dropdown-toggle" data-toggle="dropdown" href="https://2017.igem.org/Team:East_Chapel_Hill/Results">Results
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          <li><a href="https://2017.igem.org/Team:East_Chapel_Hill/Results">Results and Future Directions</a></li>
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          <li><a href="https://2017.igem.org/Team:East_Chapel_Hill/Demonstrate">Demonstrate</a></li>
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          <li><a href="https://2017.igem.org/Team:East_Chapel_Hill/Measurement">Measurement</a></li>
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          <li><a href="https://2017.igem.org/Team:East_Chapel_Hill/HP/Silver">HP Silver</a></li>
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          <li><a href="https://2017.igem.org/Team:East_Chapel_Hill/HP/Gold_Integrated">HP Gold</a></li>
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          <li><a href="https://2017.igem.org/Team:East_Chapel_Hill/Engagement">Engagement</a></li>       
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          <li><a href="https://2017.igem.org/Team:East_Chapel_Hill/Collaborations">Collaborations</a></li>
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<h1> Demonstration That Our Part Works!</h1>
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Our project pertains to measuring concentrations of fluoride and characterizing fluoride riboswitches with higher affinity to fluoride; these technologies can be used to determine methods to sequester, bioremediate, and detect fluoride.<br>
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We developed the "Fluoride Riboswitch Regulated Chloramphenicol Acetyltransferase Operon" (CHOP) regulated by the fluoride riboswitch as a system to characterize the fluoride riboswitch. <br>
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Since bacteria does not grow in the presence of chloramphenicol, the protein bacteria needs the chloramphenicol acetyltransferase protein in order to grow on chloramphenicol. We plated serial dilutions of CHOP and ΔcrcB, which is our control <i>E. coli</i> without the resistance to chloramphenicol, onto plates with varying concentrations of fluoride in order to test the best level the fluoride that CHOP can stimulate growth. <br>
 
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<h5> Special track teams </h5>
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Our results demonstrated that with no fluoride there only growth of CHOP containing bacteria on chloramphenicol plates at the highest dilutions <b>TOP</b> and no growth when plated at lower density <b>BOTTOM</b>. However, as the fluoride concentration of the plates increased, the growth of CHOP containing bacteria also increased. In other words, CHOP's ability to allow <i>E. coli</i> to grow on chloramphenicol is fluoride dependent. This result demonstrates that the fluoride riboswitch was able to regulate the expression of chloramphenicol acetyltransferase as intended; the concentration with the most growth of CHOP would represent the best activating concentration of fluoride for the riboswitch. Since the growth of bacteria containing the CHOP vector is correlated with the activation of the fluoride riboswitch, CHOP can be used to find other riboswitches that have a better affinity to fluoride, by screening for fluoride-dependent bacterial growth at lower concentrations of fluoride.<br><br>
  
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Special track teams can achieve this medal criterion by bringing their work to the Jamboree and showcasing it in the track event. Art & Design, Measurement, Hardware and Software tracks will all have showcase events at the Giant Jamboree.<strong> Please note biological materials must not be taken out of the lab</strong>.
 
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Latest revision as of 02:54, 2 November 2017

Demonstration That Our Part Works!

Our project pertains to measuring concentrations of fluoride and characterizing fluoride riboswitches with higher affinity to fluoride; these technologies can be used to determine methods to sequester, bioremediate, and detect fluoride.
We developed the "Fluoride Riboswitch Regulated Chloramphenicol Acetyltransferase Operon" (CHOP) regulated by the fluoride riboswitch as a system to characterize the fluoride riboswitch.
Since bacteria does not grow in the presence of chloramphenicol, the protein bacteria needs the chloramphenicol acetyltransferase protein in order to grow on chloramphenicol. We plated serial dilutions of CHOP and ΔcrcB, which is our control E. coli without the resistance to chloramphenicol, onto plates with varying concentrations of fluoride in order to test the best level the fluoride that CHOP can stimulate growth.


Our results demonstrated that with no fluoride there only growth of CHOP containing bacteria on chloramphenicol plates at the highest dilutions TOP and no growth when plated at lower density BOTTOM. However, as the fluoride concentration of the plates increased, the growth of CHOP containing bacteria also increased. In other words, CHOP's ability to allow E. coli to grow on chloramphenicol is fluoride dependent. This result demonstrates that the fluoride riboswitch was able to regulate the expression of chloramphenicol acetyltransferase as intended; the concentration with the most growth of CHOP would represent the best activating concentration of fluoride for the riboswitch. Since the growth of bacteria containing the CHOP vector is correlated with the activation of the fluoride riboswitch, CHOP can be used to find other riboswitches that have a better affinity to fluoride, by screening for fluoride-dependent bacterial growth at lower concentrations of fluoride.