Difference between revisions of "Team:Paris Bettencourt/Overview"

 
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<div id=header1 class="header">OVERVIEW</div>
  
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<div class=block><div class=leftparagraphe>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nam sit amet sodales ex. Sed tempus nisl at diam tempus facilisis. In pellentesque vulputate erat a vulputate. In maximus risus et accumsan scelerisque. Nullam vel porttitor metus. Donec varius risus eu urna sodales, ac convallis metus faucibus. Sed quis sapien tellus. Cras efficitur arcu et tellus vehicula maximus. Ut tincidunt dui ut sollicitudin malesuada. Quisque maximus, ex in ultrices tristique, purus felis ultrices mauris, ut sagittis lorem turpis et lorem. Etiam quis arcu aliquam, molestie risus vel, aliquam sapien. Ut metus arcu, sodales eget ex ac, lobortis venenatis lorem. Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Vivamus tempus mollis lorem, eget accumsan orci venenatis in. Fusce id sapien mi. Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nam et magna orci. Sed sodales ante elit, id porttitor arcu rutrum in. Nunc porta non tortor eu rutrum. Praesent mollis tincidunt tortor id sodales. Mauris nibh arcu, tincidunt sollicitudin mi nec, volutpat blandit sem. Aenean condimentum ante non interdum tincidunt. In vel nisl nulla. Morbi sodales massa eget justo tempus, sit amet tristique libero. </div><img class=rightimg src=""></div>
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<div class=textbody><h1>Why do we need 3D control?</h1>
  
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<div class=block><img class=leftimg src=""><div class=rightparagraphe>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Donec consectetur vitae enim suscipit rutrum. Phasellus aliquam, mi molestie pretium porta, orci turpis condimentum leo, sit amet consequat dui est vitae lorem. Morbi enim velit, euismod at leo malesuada, malesuada tempor justo. Cras vel augue nec leo hendrerit scelerisque. Praesent venenatis nunc sed tellus lacinia sodales. Pellentesque non sagittis magna, ac aliquet lacus. Suspendisse eu eros nisl. Nunc tincidunt tempor mauris. Vestibulum condimentum ac arcu ac pretium. Cras fermentum efficitur tristique. Fusce ac nibh a ex hendrerit vulputate. Aliquam aliquet a felis vitae ullamcorper. Suspendisse pellentesque libero id mi commodo ultrices. Nullam vel nisi dapibus, convallis ex sit amet, rhoncus mi. Etiam nec diam fermentum nulla vulputate volutpat vitae eu nulla. Pellentesque non dignissim arcu, accumsan dapibus ex. Donec rhoncus orci et quam maximus tempor. Vestibulum malesuada dui at dapibus dictum. Duis convallis faucibus sem eu iaculis. Vestibulum vel arcu ex. Sed porta lacus ut libero imperdiet, pellentesque tempor ante luctus. Mauris porta augue dui, sed commodo magna cursus vitae. Phasellus sollicitudin, ex in imperdiet dictum, mauris diam tempus libero, vel tincidunt nulla magna in enim. Donec et leo malesuada, sodales mi ac, vestibulum massa. Suspendisse potenti. Curabitur dignissim lorem id. </div></div>
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<div class =text1> Life exists in three dimensions but oftentimes, life sciences research remains very flat. Our limitation to control precise behaviour in a 3D space, abstracts what we can do and distances us from in vivo. Having power ful tools in the 3D allows us to both study and control life with more accuracy. </div>
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                <div class=text1><h1>A Bacterial 3D printer</h1></div>
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                <div class=text2left>To best demonstrate our 3D control tools, we created a intuitive way to bring our technologies into people’s every day life. We created a bacterial 3D printer, where we developed and characterise optogenetic tools in bacteria to produce a biomaterial when activated. This is a demonstration of our work where we showcase spatial control with a final product. This also allowed us to further characterise biomaterial </div>
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              <div class=text2right> production in micro-organisms. We chose to focus on three biomaterials: Calcium Carbonate, Poly-silicate and Poly-hydroalkanoates (PHA). The system also allows us to apply intracellular 3D control, which we achieve thanks to designing synthetic RNA organelles in the cell where local enzyme concentrations are generated. Thus, to perfect our 3D printer we focused on several main axes of research.  </div>
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<div class=text2left><h1>Optogenentic Control</h1>
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The Medusa optic control system activates bacteria in a target point with two light beams. Thanks to two photoreceptors integrated in an And-Gate logic, only the bacteria located at the intersection of the beams become activated. We implemented two kinds of photoreceptors: a couple of the <a href="https://2017.igem.org/Team:Paris_Bettencourt/Transmembrane_Proteins"><b>classical membrane photosensors</b></a> and two more recent <a href="https://2017.igem.org/Team:Paris_Bettencourt/Transmembrane_Proteins"><b> photocaging fluorescent proteins</b></a>. We processed the inhibitory input of our photosensors thanks to <a href="https://2017.igem.org/Team:Paris_Bettencourt/Logic_Circuit"><b>a newly designed Nor-Gate</b></a> .</div>
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To demonstrate the power of our control system, we placed the synthesis of three biomaterials downstream of our light sensing circuit. This turns our light controlled bacteria in a 3D-bioprinter able to print <a ref="https://2017.igem.org/Team:Paris_Bettencourt/Biomaterials#header2"><b> bioplastic </b></a> , <a href="https://2017.igem.org/Team:Paris_Bettencourt/Biomaterials#header4"><b> bioglass </b></a>  and, <a href="https://2017.igem.org/Team:Paris_Bettencourt/Biomaterials#header3"><b> limestone </b></a>.
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<div class=text2left><h1>RNA organelle</h1>RNA is a light cost nucleotide material in the cell,
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Latest revision as of 03:12, 2 November 2017

OVERVIEW

Why do we need 3D control?

Life exists in three dimensions but oftentimes, life sciences research remains very flat. Our limitation to control precise behaviour in a 3D space, abstracts what we can do and distances us from in vivo. Having power ful tools in the 3D allows us to both study and control life with more accuracy.

A Bacterial 3D printer

To best demonstrate our 3D control tools, we created a intuitive way to bring our technologies into people’s every day life. We created a bacterial 3D printer, where we developed and characterise optogenetic tools in bacteria to produce a biomaterial when activated. This is a demonstration of our work where we showcase spatial control with a final product. This also allowed us to further characterise biomaterial
production in micro-organisms. We chose to focus on three biomaterials: Calcium Carbonate, Poly-silicate and Poly-hydroalkanoates (PHA). The system also allows us to apply intracellular 3D control, which we achieve thanks to designing synthetic RNA organelles in the cell where local enzyme concentrations are generated. Thus, to perfect our 3D printer we focused on several main axes of research.

Optogenentic Control

The Medusa optic control system activates bacteria in a target point with two light beams. Thanks to two photoreceptors integrated in an And-Gate logic, only the bacteria located at the intersection of the beams become activated. We implemented two kinds of photoreceptors: a couple of the classical membrane photosensors and two more recent photocaging fluorescent proteins. We processed the inhibitory input of our photosensors thanks to a newly designed Nor-Gate .

Biomaterials

To demonstrate the power of our control system, we placed the synthesis of three biomaterials downstream of our light sensing circuit. This turns our light controlled bacteria in a 3D-bioprinter able to print bioplastic , bioglass and, limestone .

RNA organelle

RNA is a light cost nucleotide material in the cell, We aim to recreate RNA agglomerations as formed in mammalian cells with triple repeat disorders, which show liquid phase separation, forming a organelle-like vesicle, where local concentrations of enzymes can be created.


Centre for Research and Interdisciplinarity (CRI)
Faculty of Medicine Cochin Port-Royal, South wing, 2nd floor
Paris Descartes University
24, rue du Faubourg Saint Jacques
75014 Paris, France
bettencourt.igem2017@gmail.com