Difference between revisions of "Team:Heidelberg/Achievements"

 
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<div style="font-size: 25px !important; font-weight: 700 !important; padding-bottom: 50px !important">We learned a lot, found new friends and had great fun over the summer. In addition, we...</div>
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  <img src="https://static.igem.org/mediawiki/2017/5/53/T--Heidelberg--2017_PACE_AiGEM.jpg" style="width: 100%; height: auto;">
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  <div class="col-lg-10 col-md-10 col-xs-6" style="vertical-align: center; font-weight: 500 !important">
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Introduced a unique in vivo and <i>in silico</i> directed evolution interface as novel engineering paradigm to synthetic biology.</div>
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    </div>
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  <div class="heidel-form row">
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  <div class="col-lg-2 col-md-2 col-xs-6">
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  <img src="https://static.igem.org/mediawiki/2017/8/85/T--Heidelberg--2017_Pretzel.jpg" style="width: 100%; height: auto;">
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  </div>
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  <div class="col-lg-10 col-md-10 col-xs-6" style="vertical-align: center; font-weight: 500 !important">
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Developed PREDCEL, as simple, cheap and easy-to-implement PACE alternative.</div>
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    </div>
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  <div class="heidel-form row">
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  <div class="col-lg-2 col-md-2 col-xs-6">
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  <img src="https://static.igem.org/mediawiki/2017/3/3c/T--Heidelberg--2017_RiboswitchIcon.jpg" style="width: 100%; height: auto;">
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  </div>
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  <div class="col-lg-10 col-md-10 col-xs-6" style="vertical-align: center; font-weight: 500 !important">
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Outlined a unique workflow for in vivo enzyme evolution based on computationally predicted riboswitches.</div>
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    </div>
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  <div class="heidel-form row">
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  <div class="col-lg-2 col-md-2 col-xs-6">
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  <img src="https://static.igem.org/mediawiki/2017/d/d8/T--Heidelberg--2017.jpeg" style="width: 100%; height: auto;">
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  </div>
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  <div class="col-lg-10 col-md-10 col-xs-6" style="vertical-align: center; font-weight: 500 !important">
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First iGEM team to use an engineered cytochrome to catalyze carbon-silicon bond formation in whole cell extracts.</div>
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    </div>
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  <div class="heidel-form row">
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  <div class="col-lg-2 col-md-2 col-xs-6">
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  <img src="https://static.igem.org/mediawiki/2017/5/5e/T--Heidelberg--2017_Human_Practice2.png" style="width: 100%; height: auto;">
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  </div>
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  <div class="col-lg-10 col-md-10 col-xs-6" style="vertical-align: center; font-weight: 500 !important">
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Successfully integrated the feedback of experts and the broader public into our project design at diverse layers.</div>
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    </div>
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  <div class="heidel-form row">
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  <div class="col-lg-2 col-md-2 col-xs-6">
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  <img src="https://static.igem.org/mediawiki/2017/4/4a/T--Heidelberg--2017_LogoBackgr.jpg" style="width: 100%; height: auto;">
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  </div>
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  <div class="col-lg-10 col-md-10 col-xs-6" style="vertical-align: center; font-weight: 500 !important">
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Frist iGEM team to apply deep learning to protein engineering.</div>
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    </div>
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  <div class="heidel-form row">
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  <div class="col-lg-2 col-md-2 col-xs-6">
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  <img src="https://static.igem.org/mediawiki/2017/b/b7/T--Heidelberg--2017_DeeProtein_LOGO.jpg" style="width: 100%; height: auto;">
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  </div>
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  <div class="col-lg-10 col-md-10 col-xs-6" style="vertical-align: center; font-weight: 500 !important">
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Created and validated AiGEM, an intelligent software for generating protein functionality de novo.</div>
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    </div>
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  <div class="heidel-form row">
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  <div class="col-lg-2 col-md-2 col-xs-6">
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  <img src="https://static.igem.org/mediawiki/2017/c/cd/T--Heidelberg--2017_SafetyNetRedIcon.jpg" style="width: 100%; height: auto;">
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  </div>
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  <div class="col-lg-10 col-md-10 col-xs-6" style="vertical-align: center; font-weight: 500 !important">
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Implemented SafetyNET to safeguard directed evolution experiments.</div>
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    </div>
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      </div>
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</div>
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                     <div class="card-body">
 
                     <div class="card-body">
 
                         <h4 class="card-title">Bronze</h4>
 
                         <h4 class="card-title">Bronze</h4>
−
                         <p class="card-text content"><i class="fa fa-check" aria-hidden="true"></i> Find a comprehensive compilation of sponsors, partners and scientific contributors on our <a href="https://2017.igem.org/Team:Heidelberg/Attributions">Attributions page</a></p>
+
                         <p class="card-text content"><i class="fa fa-check" aria-hidden="true"></i> Find a comprehensive compilation of sponsors, partners and scientific contributors on our <a href="https://2017.igem.org/Team:Heidelberg/Attributions" style="color: #CD7F32 !important">Attributions page</a></p>
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                         <p class="card-text content"><i class="fa fa-check" aria-hidden="true"></i> We submitted <a href="https://2017.igem.org/Team:Heidelberg/Parts#Part_List">94 parts</a> as part of our <a href="https://2017.igem.org/Team:Heidelberg/Toolbox">PREDCEL/PACE toolbox</a> with the necessary documentation.</p>
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                         <p class="card-text content"><i class="fa fa-check" aria-hidden="true"></i> We submitted <a href="https://2017.igem.org/Team:Heidelberg/Parts#Part_List" style="color: #CD7F32 !important">94 parts</a> as part of our <a href="https://2017.igem.org/Team:Heidelberg/Toolbox" style="color: #CD7F32 !important">PREDCEL/PACE toolbox</a> with the necessary documentation.</p>
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                         <p class="card-text content"><i class="fa fa-check" aria-hidden="true"></i> Find <a href="https://2017.igem.org/Team:Heidelberg/InterLab">our results for the InterLab Measurement Study</a></p>
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                         <p class="card-text content"><i class="fa fa-check" aria-hidden="true"></i> Find <a href="https://2017.igem.org/Team:Heidelberg/InterLab" style="color: #CD7F32 !important">our results for the InterLab Measurement Study</a></p>
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                         <p class="card-text content"><i class="fa fa-check" aria-hidden="true"></i> We complied with all requirements and regulations set by the iGEM competition</a></p>
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                         <p class="card-text content"><i class="fa fa-check" aria-hidden="true"></i> We complied with all requirements and regulations set by the iGEM competition</p>
 
                         <p class="card-text content"><i class="fa fa-check" aria-hidden="true"></i> We had a great summer and are very much looking forward to meet you at the Giant Jamboree</a></p>
 
                         <p class="card-text content"><i class="fa fa-check" aria-hidden="true"></i> We had a great summer and are very much looking forward to meet you at the Giant Jamboree</a></p>
 
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                     <div class="card-body">
 
                     <div class="card-body">
 
                         <h4 class="card-title">Silver</h4>
 
                         <h4 class="card-title">Silver</h4>
−
                         <p class="card-text content"><i class="fa fa-check" aria-hidden="true"></i> We experimentally validated that our BioBricks (e.g. <a href="http://parts.igem.org/Part:BBa_K2398019">BBa_K2398019</a>, BBa_K2398555 and BBa_K2398557) work as expected! Visit our toolbox collection for more than 90 parts</p>
+
                         <p class="card-text content"><i class="fa fa-check" aria-hidden="true"></i> We experimentally validated that our BioBricks (e.g. <a href="http://parts.igem.org/Part:BBa_K2398019" style="color: #C0C0C0 !important">BBa_K2398019</a>, <a href="http://parts.igem.org/Part:BBa_K2398555" style="color: #C0C0C0 !important">BBa_K2398555</a> and <a href="http://parts.igem.org/Part:BBa_K2398557" style="color: #C0C0C0 !important">BBa_K2398557</a>) work as expected! Visit our toolbox collection for more than 90 parts</p>
−
                         <p class="card-text content"><i class="fa fa-check" aria-hidden="true"></i> We collaborated with other iGEM teams from all over the world in a bi-directional and significant way. We took over complicated clonings for the iGEM team Freiburg and initiated our own Heidelberg Interlab Study to validate our Mutagenesis Plasmids.</p>
+
                         <p class="card-text content"><i class="fa fa-check" aria-hidden="true"></i> We collaborated with other iGEM teams from all over the world in a bi-directional and significant way. We initiated our own Heidelberg Interlab Study to validate our Mutagenesis Plasmids.</p>
 
  <p class="card-text content"><i class="fa fa-check" aria-hidden="true"></i> We critically thought about the impact of our project on society, environment and safety. To this end, we engaged with the public, experts and pupils.  </p>
 
  <p class="card-text content"><i class="fa fa-check" aria-hidden="true"></i> We critically thought about the impact of our project on society, environment and safety. To this end, we engaged with the public, experts and pupils.  </p>
 
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                         <p class="card-text content"><i class="fa fa-check" aria-hidden="true"></i> We improved the function of an existing biobrick by calculated improved beta-lactamase variants in silico and evaluating the superior functionality in the wet lab. </p>
 
                         <p class="card-text content"><i class="fa fa-check" aria-hidden="true"></i> We improved the function of an existing biobrick by calculated improved beta-lactamase variants in silico and evaluating the superior functionality in the wet lab. </p>
 
  <p class="card-text content"><i class="fa fa-check" aria-hidden="true"></i> We intensively modeled our PREDCEL experiments and provide web-based interactive tools that enable our users to model their own systems!  </p>
 
  <p class="card-text content"><i class="fa fa-check" aria-hidden="true"></i> We intensively modeled our PREDCEL experiments and provide web-based interactive tools that enable our users to model their own systems!  </p>
−
<p class="card-text content"><i class="fa fa-check" aria-hidden="true"></i> We developed PREDCEL to enable phage-assisted directed evolution in batch mode that is much easier to handle and less expensive compared to the original PACE method. We demonstrate the feasibility of our technology on a protein-interaction experiment based on the split T7 polymerase and in evolving the enzyme cytochrome P450 1A2. </p>
+
<p class="card-text content"><i class="fa fa-check" aria-hidden="true"></i> We developed PREDCEL as easy-to-handle and cheap PACE alternative. We demonstrate the feasibility of our technology by several applications including CYP1A2. </p>
 
                     </div>
 
                     </div>
 
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                 </div>

Latest revision as of 03:32, 2 November 2017

Achievements
The essence of our work
We learned a lot, found new friends and had great fun over the summer. In addition, we...
Introduced a unique in vivo and in silico directed evolution interface as novel engineering paradigm to synthetic biology.
Developed PREDCEL, as simple, cheap and easy-to-implement PACE alternative.
Outlined a unique workflow for in vivo enzyme evolution based on computationally predicted riboswitches.
First iGEM team to use an engineered cytochrome to catalyze carbon-silicon bond formation in whole cell extracts.
Successfully integrated the feedback of experts and the broader public into our project design at diverse layers.
Frist iGEM team to apply deep learning to protein engineering.
Created and validated AiGEM, an intelligent software for generating protein functionality de novo.
Implemented SafetyNET to safeguard directed evolution experiments.
.
Card image cap

Bronze

Find a comprehensive compilation of sponsors, partners and scientific contributors on our Attributions page

We submitted 94 parts as part of our PREDCEL/PACE toolbox with the necessary documentation.

Find our results for the InterLab Measurement Study

We complied with all requirements and regulations set by the iGEM competition

We had a great summer and are very much looking forward to meet you at the Giant Jamboree

Card image cap

Silver

We experimentally validated that our BioBricks (e.g. BBa_K2398019, BBa_K2398555 and BBa_K2398557) work as expected! Visit our toolbox collection for more than 90 parts

We collaborated with other iGEM teams from all over the world in a bi-directional and significant way. We initiated our own Heidelberg Interlab Study to validate our Mutagenesis Plasmids.

We critically thought about the impact of our project on society, environment and safety. To this end, we engaged with the public, experts and pupils.

Card image cap

Gold

We extended the Engineering Cycle for Foundational Advance projects by including sophisticated concepts for safety measures and the responsible use of new technologies.

We improved the function of an existing biobrick by calculated improved beta-lactamase variants in silico and evaluating the superior functionality in the wet lab.

We intensively modeled our PREDCEL experiments and provide web-based interactive tools that enable our users to model their own systems!

We developed PREDCEL as easy-to-handle and cheap PACE alternative. We demonstrate the feasibility of our technology by several applications including CYP1A2.