Difference between revisions of "Team:William and Mary/Part Collection"

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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<h1> Part Collection </h1>
 
  
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<p>Did your team make a lot of great parts? Is there a theme that ties all your parts together? Do you have more than 10 parts in this collection? Did you make a CRISPR collection, a MoClo collection, or a collection of awesome pigment parts? Describe your parts collection on this page, so the judges can evaluate you for the Best Part Collection award.</p>
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<p>
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<center><img src="https://static.igem.org/mediawiki/2017/3/30/T--William_and_Mary--partcollect.jpeg" width="420px"/></center>
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While you should put all the characterization information for your parts on the Registry, you are encouraged to explain how all your parts form a collection on this page.
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</p>
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<br>
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<h3>Best Part Collection Special Prize</h3>
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<p>Did your team make a lot of great parts? Is there a team that ties all your parts together? Do you have more than 10 parts in this collection? Did you make a CRISPR collection, a MoClo collection or a collection of awesome pigment parts? Tell the judges you should be evaluated for the Best Parts Collection award! To be eligible for this award, these parts must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts.
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<br><br>
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If you have a collection of parts you wish to nominate your team for this <a href="https://2017.igem.org/Judging/Awards">special prize</a>, make sure you add your part numbers to your <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.</p>
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<div style = 'padding-left: 14%; padding-bottom: 10px;font-size: 25px' ><b>Modular Protein Degradation System for Speed Control</b></div>
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<h4>Note</h4>
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<p>This page should list all the parts in the collection your team made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page.</p>
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Our part collection provides future iGEM teams with the basic toolkit required to achieve modular, predictive speed control over their genetic circuits. We provide six distinct protein degradation tags (pdt) with distinct strengths and hence distinct effects on the speed of a tagged protein’s expression, in a ready-to-clone format for immediate use. We also provide constitutive and inducible reporter constructs tagged with each of the six tags. In total, this part collection represents the first experimental confirmation of a foundational mathematical model, as well as an accessible system that will allow future iGEM teams to access modular, predictive control over the temporal dynamics of their circuits by swapping parts at the genetic sequence level. We hope that our pdt system will, in addition to enabling this first foray into circuit speed control, serve as a foundation to drive continued innovations in controlling the dynamical properties of circuits.
  
 
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For more information on these, and the rest of our composite parts please see our <a href = "https://2017.igem.org/Team:William_and_Mary/Parts" style='text-decoration: underline;'>parts</a> page. We'd also like to ask that Judges consider these parts as fufillment of silver medal criteria I. For more information please see our <a href='https://2017.igem.org/Team:William_and_Mary/For_Judges' style='text-decoration: underline;'>for judges</a> page.
 
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<div style='padding-top: 50px;'></div>
  
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<div style = 'padding-left: 14%; padding-bottom: 10px;font-size: 25px' ><b>Part Collection</b></div>
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<table style='width: 75%; margin: 0px 14% 20px 14%;'>
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<thead>
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  <tr>
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<th>BioBrick ID</th>
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<th>Construct</th>
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  </tr>
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  </thead>
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  <tbody>
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  <tr>
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<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2333401"> K2333401 </a></td>
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<td>Cloning Ready pdt A with Double Terminator
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</td>
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  </tr>
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  <tr>
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<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2333402"> K2333402 </a></td>
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<td>Cloning Ready pdt B with Double Terminator</td>
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  </tr>
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    <tr>
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<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2333403"> K2333403 </a></td>
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<td>Cloning Ready pdt C with Double Terminator </td>
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  </tr>
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  <tr>
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<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2333404"> K2333404 </a></td>
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<td> Cloning Ready pdt D with Double Terminator </td>
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  </tr>
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  <tr>
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<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2333405"> K2333405 </a></td>
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<td>Cloning Ready pdt E with Double Terminator </td>
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  </tr>
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          <tr>
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<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2333406"> K2333406 </a></td>
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        <td>Cloning Ready pdt F with Double Terminator </td>
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  </tr>
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<tr>
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<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2333413"> K2333413 </a></td>
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        <td>UNS J23100 mScarlet-I</td>
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  </tr>
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<tr>
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<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2333414"> K2333414</a></td>
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        <td>UNS J23100 mScarlet-I pdt A</td>
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  </tr>
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<tr>
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<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2333415"> K2333415</a></td>
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        <td>UNS J23100 mScarlet-I pdt B</td>
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  </tr>
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<tr>
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<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2333416"> K2333416</a></td>
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        <td>UNS J23100 mScarlet-I pdt C</td>
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  </tr>
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<tr>
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<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2333417"> K2333417</a></td>
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        <td>UNS J23100 mScarlet-I pdt D</td>
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  </tr>
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<tr>
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<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2333418"> K2333418</a></td>
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        <td>UNS J23100 mScarlet-I pdt E</td>
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  </tr>
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<tr>
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<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2333419"> K2333419</a></td>
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        <td>UNS J23100 mScarlet-I pdt F</td>
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  </tr>
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<tr>
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<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2333427"> K2333427</a></td>
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        <td>UNS pTet mScarlet-I</td>
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  </tr>
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<tr>
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<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2333428"> K2333428</a></td>
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        <td>UNS pTet mScarlet-I pdt A</td>
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  </tr>
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<tr>
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<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2333429"> K2333429</a></td>
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        <td>UNS pTet mScarlet-I pdt B</td>
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  </tr>
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<tr>
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<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2333430"> K2333430</a></td>
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        <td>UNS pTet mScarlet-I pdt C</td>
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  </tr>
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<tr>
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<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2333431"> K2333431</a></td>
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        <td>UNS pTet mScarlet-I pdt D</td>
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  </tr>
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<tr>
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<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2333432"> K2333432</a></td>
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        <td>UNS pTet mScarlet-I pdt E</td>
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  </tr>
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<tr>
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<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2333433"> K2333433</a></td>
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        <td>UNS pTet mScarlet-I pdt F</td>
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  </tr>
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<tr>
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<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2333434"> K2333434</a></td>
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        <td>UNS plac mf-Lon</td>
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  </tr>
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<tr>
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<td><a href = "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2333435"> K2333435</a></td>
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        <td>UNS pBad mf-Lon</td>
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  </tr>
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  </tbody>
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</table>
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Latest revision as of 03:44, 2 November 2017



Modular Protein Degradation System for Speed Control
Our part collection provides future iGEM teams with the basic toolkit required to achieve modular, predictive speed control over their genetic circuits. We provide six distinct protein degradation tags (pdt) with distinct strengths and hence distinct effects on the speed of a tagged protein’s expression, in a ready-to-clone format for immediate use. We also provide constitutive and inducible reporter constructs tagged with each of the six tags. In total, this part collection represents the first experimental confirmation of a foundational mathematical model, as well as an accessible system that will allow future iGEM teams to access modular, predictive control over the temporal dynamics of their circuits by swapping parts at the genetic sequence level. We hope that our pdt system will, in addition to enabling this first foray into circuit speed control, serve as a foundation to drive continued innovations in controlling the dynamical properties of circuits.
For more information on these, and the rest of our composite parts please see our parts page. We'd also like to ask that Judges consider these parts as fufillment of silver medal criteria I. For more information please see our for judges page.
Part Collection
BioBrick ID Construct
K2333401 Cloning Ready pdt A with Double Terminator
K2333402 Cloning Ready pdt B with Double Terminator
K2333403 Cloning Ready pdt C with Double Terminator
K2333404 Cloning Ready pdt D with Double Terminator
K2333405 Cloning Ready pdt E with Double Terminator
K2333406 Cloning Ready pdt F with Double Terminator
K2333413 UNS J23100 mScarlet-I
K2333414 UNS J23100 mScarlet-I pdt A
K2333415 UNS J23100 mScarlet-I pdt B
K2333416 UNS J23100 mScarlet-I pdt C
K2333417 UNS J23100 mScarlet-I pdt D
K2333418 UNS J23100 mScarlet-I pdt E
K2333419 UNS J23100 mScarlet-I pdt F
K2333427 UNS pTet mScarlet-I
K2333428 UNS pTet mScarlet-I pdt A
K2333429 UNS pTet mScarlet-I pdt B
K2333430 UNS pTet mScarlet-I pdt C
K2333431 UNS pTet mScarlet-I pdt D
K2333432 UNS pTet mScarlet-I pdt E
K2333433 UNS pTet mScarlet-I pdt F
K2333434 UNS plac mf-Lon
K2333435 UNS pBad mf-Lon