Difference between revisions of "Team:TU Dresden/Experiments"

 
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{{TU_Dresden/Header}}
 
{{TU_Dresden/Header}}
 
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<div id="pagebanner" style="background-image: url(https://static.igem.org/mediawiki/2017/3/32/TU_Dresden_LabWork_Header.jpeg);">
 
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   <div id="bannerquote" style="text-shadow: 0 0 3px var(--logo-dark-blue);">Don´t try this at home!</div>
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   <div id="bannerquote" style="text-shadow: 0 0 3px var(--logo-dark-blue);">Don´t try this at home</div>
 
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<main>
 
<main>
  
 
<div class="contentbox">
 
<div class="contentbox">
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<h1 class="box-heading"> Protocols</h1>
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<h1 class="box-heading">Peptidosome Methods </h1>
  
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<h2> Cloning </h2>
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<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/8/87/Protocols_peptidosomes.pdf">Peptidosome Creation</a>
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<a class="pdf-resources" "https://static.igem.org/mediawiki/2017/e/e0/T--TU_Dresden--M_Cloning.pdf">Cloning procedure</a>
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<a class="pdf-resources" href="https://static.igem.org/mediawiki/2017/6/6d/T--TU_Dresden--M_TransformationCaCl.pdf">Transformation CaCl method</a>
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<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/f/f6/T--TU_Dresden--EncapsulationofBsubtilis.pdf">Encapsulation of <i>B. subtilis</i></a>
  
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<a class="pdf-resources" href="https://static.igem.org/mediawiki/2017/0/09/T--TU_Dresden--M_OneTaqPCR.pdf">PCR with One Taq Polymerase</a>
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<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/4/41/Wellscan.pdf">Plate Reader Wellscan Peptidosomes</a>
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</div>
  
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<a class="pdf-resources" href="https://static.igem.org/mediawiki/2017/1/17/T--TU_Dresden--M_Q5PCR.pdf">PCR with Q5 Polymerase</a>
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<div class="contentbox">
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<h1 class="box-heading"> Cloning and Primer Lists</h1>
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<h2>Cloning</h2>
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<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/d/dc/New_cloning.pdf">Cloning Procedure</a>
  
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<a class="pdf-resources" href="https://static.igem.org/mediawiki/2017/b/bf/T--TU_Dresden--M_LFH.pdf">Long flanking homology PCR</a>
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<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/3/39/Transformations.pdf">Transformation CaCl Method</a>
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<p>
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<hr>
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<h2> Working with <i>Bacillus subtilis</i></h2>
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<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/0/09/T--TU_Dresden--M_OneTaqPCR.pdf">PCR with One Taq Polymerase</a>
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<a class="pdf-resources" "https://static.igem.org/mediawiki/2017/3/3c/T--TU_Dresden--M_DNA_Isolation.pdf">DNA isolation (for PCR)</a>
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<a class="pdf-resources" href="https://static.igem.org/mediawiki/2017/f/f7/T--TU_Dresden--M_IsolationDNATrafo.pdf">DNA isolation (for transformation)</a>
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<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/1/17/T--TU_Dresden--M_Q5PCR.pdf">PCR with Q5 Polymerase</a>
  
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<a class="pdf-resources" href="https://static.igem.org/mediawiki/2017/5/5e/T--TU_Dresden--M_Transformation_Bacillus.pdf">Transformation of <i>B. subtilis </i></a>
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<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/b/bf/T--TU_Dresden--M_LFH.pdf">Long Flanking Homology PCR</a>
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<p>
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<hr>
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<h2>Media and Solutions </h2>
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<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/d/db/SOP_protocols_for_cloning_with_the_Evaluation_Vector_and_the_Signal_Peptide_Toolbox.pdf">SOP Protocols for Cloning with the Evaluation Vector and the Signal Peptide Toolbox</a>
  
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<a class="pdf-resources" "https://static.igem.org/mediawiki/2017/9/9d/T--TU_Dresden--M_Media_Antibiotics.pdf">Media, Solutions & Antibiotics</a>
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<h2>Primer</h2>
  
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<a class="pdf-resources" href="https://static.igem.org/mediawiki/2017/f/f2/T--TU_Dresden--M_JensensMedia.pdf">Jensen's Medium</a>
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<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/0/0f/Primer_list.pdf">Primer List General</a>
  
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<a class="pdf-resources" href="https://static.igem.org/mediawiki/2017/4/45/T--TU_Dresden--M_MCSE.pdf">MCSE Medium</a>
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<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/b/b8/Primer_list_signal_peptides.pdf">Primer List Signal Peptides</a>
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<p>
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<hr>
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<h2>Primer</h2>
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</div>
  
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<a class="pdf-resources" href="https://static.igem.org/mediawiki/2017/b/b9/T--TU_Dresden--M_Primerlist.pdf">Primerlist general</a>
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<div class="contentbox">
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<h1 class="box-heading">Working with <i>Bacillus subtilis</i></h1>
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<a class="pdf-resources" target="_blank" href= "https://static.igem.org/mediawiki/2017/0/05/Dna_isolation_for_pcr.pdf">DNA Isolation (for PCR)</a>
  
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<a class="pdf-resources" href="https://static.igem.org/mediawiki/2017/9/9b/T--TU_Dresden--M_Primerlist_Signalpeptides.pdf">Primerlist Signalpeptides</a>
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<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/4/4c/Dna_isolation_for_transformation.pdf">DNA Isolation (for Transformation)</a>
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<p>
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<hr>
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<h2>Assays</h2>
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<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/5/5f/Bacillus_transformation.pdf">Transformation of <i>B. subtilis </i></a>
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<a class="pdf-resources" href="https://static.igem.org/mediawiki/2017/0/07/T--TU_Dresden--M_DiscDiffusion.pdf">Disc Diffussion Assay</a>
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</div>
  
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<a class="pdf-resources" href="https://static.igem.org/mediawiki/2017/7/71/T--TU_Dresden--M_PR_Luminescence.pdf">PR Luminescence Assay</a>
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<div class="contentbox">
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<p>
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<h1 class="box-heading">Media and Solutions </h1>
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<hr>
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<h2>Protein work</h2>
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<a class="pdf-resources" target="_blank" href= "https://static.igem.org/mediawiki/2017/6/62/Media_antibiotics.pdf">Media, Solutions & Antibiotics</a>
  
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<a class="pdf-resources" href="https://static.igem.org/mediawiki/2017/8/81/T--TU_Dresden--M_Proteins.pdf">His-Tag purification with agarose beads</a>
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<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/2/29/Jensens_media.pdf">Jensen's Medium</a>
  
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<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/0/03/Mcse.pdf">MCSE Medium</a>
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</div>
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<div class="contentbox">
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<h1 class="box-heading">Assays</h1>
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<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/c/c8/Disc_diffusion.pdf">Disc Diffussion Assay</a>
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<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/c/c4/Pr.pdf">Plate Reader Assay</a>
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<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/3/3b/Secretion_assay.pdf">Fluorescence Assay</a>
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</div>
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<div class="contentbox">
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<h1 class="box-heading">Protein Work</h1>
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<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/d/d9/Proteins.pdf">His-Tag Purification with Agarose Beads</a>
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<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/f/fa/Laemmli_SDS-PAGE.pdf">Laemmli SDS-PAGE</a>
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Latest revision as of 17:13, 13 December 2017