Difference between revisions of "Team:TU Dresden/Experiments"
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<div id="pagebanner" style="background-image: url(https://static.igem.org/mediawiki/2017/3/32/TU_Dresden_LabWork_Header.jpeg);"> | <div id="pagebanner" style="background-image: url(https://static.igem.org/mediawiki/2017/3/32/TU_Dresden_LabWork_Header.jpeg);"> | ||
<div id="bannerspace"> | <div id="bannerspace"> | ||
| − | <div id="bannerquote" style="text-shadow: 0 0 3px var(--logo-dark-blue);"> | + | <div id="bannerquote" style="text-shadow: 0 0 3px var(--logo-dark-blue);">Don´t try this at home</div> |
</div> | </div> | ||
<main> | <main> | ||
<div class="contentbox"> | <div class="contentbox"> | ||
| − | <h1 class="box-heading"> | + | <h1 class="box-heading">Peptidosome Methods </h1> |
| − | + | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/8/87/Protocols_peptidosomes.pdf">Peptidosome Creation</a> | |
| − | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/ | + | |
| − | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/ | + | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/f/f6/T--TU_Dresden--EncapsulationofBsubtilis.pdf">Encapsulation of <i>B. subtilis</i></a> |
| − | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/ | + | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/4/41/Wellscan.pdf">Plate Reader Wellscan Peptidosomes</a> |
| + | </div> | ||
| − | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/ | + | <div class="contentbox"> |
| + | <h1 class="box-heading"> Cloning and Primer Lists</h1> | ||
| + | <h2>Cloning</h2> | ||
| + | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/d/dc/New_cloning.pdf">Cloning Procedure</a> | ||
| − | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/ | + | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/3/39/Transformations.pdf">Transformation CaCl Method</a> |
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| − | + | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/0/09/T--TU_Dresden--M_OneTaqPCR.pdf">PCR with One Taq Polymerase</a> | |
| − | <a class="pdf-resources" target="_blank" href= "https://static.igem.org/mediawiki/2017/0/ | + | |
| − | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/ | + | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/1/17/T--TU_Dresden--M_Q5PCR.pdf">PCR with Q5 Polymerase</a> |
| − | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/ | + | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/b/bf/T--TU_Dresden--M_LFH.pdf">Long Flanking Homology PCR</a> |
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| − | < | + | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/d/db/SOP_protocols_for_cloning_with_the_Evaluation_Vector_and_the_Signal_Peptide_Toolbox.pdf">SOP Protocols for Cloning with the Evaluation Vector and the Signal Peptide Toolbox</a> |
| − | < | + | <h2>Primer</h2> |
| − | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/ | + | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/0/0f/Primer_list.pdf">Primer List General</a> |
| − | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/ | + | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/b/b8/Primer_list_signal_peptides.pdf">Primer List Signal Peptides</a> |
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| − | + | </div> | |
| − | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/0/ | + | <div class="contentbox"> |
| + | <h1 class="box-heading">Working with <i>Bacillus subtilis</i></h1> | ||
| + | <a class="pdf-resources" target="_blank" href= "https://static.igem.org/mediawiki/2017/0/05/Dna_isolation_for_pcr.pdf">DNA Isolation (for PCR)</a> | ||
| − | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/ | + | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/4/4c/Dna_isolation_for_transformation.pdf">DNA Isolation (for Transformation)</a> |
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| + | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/5/5f/Bacillus_transformation.pdf">Transformation of <i>B. subtilis </i></a> | ||
| + | </div> | ||
| + | <div class="contentbox"> | ||
| + | <h1 class="box-heading">Media and Solutions </h1> | ||
| + | <a class="pdf-resources" target="_blank" href= "https://static.igem.org/mediawiki/2017/6/62/Media_antibiotics.pdf">Media, Solutions & Antibiotics</a> | ||
| + | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/2/29/Jensens_media.pdf">Jensen's Medium</a> | ||
| + | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/0/03/Mcse.pdf">MCSE Medium</a> | ||
| + | </div> | ||
| − | < | + | <div class="contentbox"> |
| + | <h1 class="box-heading">Assays</h1> | ||
<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/c/c8/Disc_diffusion.pdf">Disc Diffussion Assay</a> | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/c/c8/Disc_diffusion.pdf">Disc Diffussion Assay</a> | ||
| − | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/c/c4/Pr.pdf">Plate | + | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/c/c4/Pr.pdf">Plate Reader Assay</a> |
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<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/3/3b/Secretion_assay.pdf">Fluorescence Assay</a> | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/3/3b/Secretion_assay.pdf">Fluorescence Assay</a> | ||
| + | </div> | ||
| − | < | + | <div class="contentbox"> |
| + | <h1 class="box-heading">Protein Work</h1> | ||
| − | + | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/d/d9/Proteins.pdf">His-Tag Purification with Agarose Beads</a> | |
| − | + | ||
| − | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/d/d9/Proteins.pdf">His-Tag | + | |
| + | <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/f/fa/Laemmli_SDS-PAGE.pdf">Laemmli SDS-PAGE</a> | ||
| + | </div> | ||
</main> | </main> | ||
Latest revision as of 17:13, 13 December 2017
