Latest revision as of 17:13, 13 December 2017

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Peptidosome Methods

Peptidosome Creation Encapsulation of B. subtilis Plate Reader Wellscan Peptidosomes

Cloning and Primer Lists

Cloning

Cloning Procedure Transformation CaCl Method PCR with One Taq Polymerase PCR with Q5 Polymerase Long Flanking Homology PCR SOP Protocols for Cloning with the Evaluation Vector and the Signal Peptide Toolbox

Primer

Primer List General Primer List Signal Peptides

Working with Bacillus subtilis

DNA Isolation (for PCR) DNA Isolation (for Transformation) Transformation of B. subtilis

Media and Solutions

Media, Solutions & Antibiotics Jensen's Medium MCSE Medium

Assays

Disc Diffussion Assay Plate Reader Assay Fluorescence Assay

Protein Work

His-Tag Purification with Agarose Beads Laemmli SDS-PAGE

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 <div class="contentbox">
-<h1 class="box-heading"> Protocols</h1>
+<h1 class="box-heading">Peptidosome Methods </h1>
-<h2> Cloning </h2>
+<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/8/87/Protocols_peptidosomes.pdf">Peptidosome Creation</a>
-<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/d/dc/New_cloning.pdf">Cloning procedure</a>
-<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/3/39/Transformations.pdf">Transformation CaCl method</a>
+<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/f/f6/T--TU_Dresden--EncapsulationofBsubtilis.pdf">Encapsulation of <i>B. subtilis</i></a>
-<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/0/09/T--TU_Dresden--M_OneTaqPCR.pdf">PCR with One Taq Polymerase</a>
+<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/4/41/Wellscan.pdf">Plate Reader Wellscan Peptidosomes</a>
+</div>
-<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/1/17/T--TU_Dresden--M_Q5PCR.pdf">PCR with Q5 Polymerase</a>
+<div class="contentbox">
+<h1 class="box-heading"> Cloning and Primer Lists</h1>
+<h2>Cloning</h2>
+<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/d/dc/New_cloning.pdf">Cloning Procedure</a>
-<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/b/bf/T--TU_Dresden--M_LFH.pdf">Long flanking homology PCR</a>
+<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/3/39/Transformations.pdf">Transformation CaCl Method</a>
-<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/d/db/SOP_protocols_for_cloning_with_the_Evaluation_Vector_and_the_Signal_Peptide_Toolbox.pdf">SOP protocols for cloning with the Evaluation Vector and the Signal Peptide Toolbox</a>
+<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/0/09/T--TU_Dresden--M_OneTaqPCR.pdf">PCR with One Taq Polymerase</a>
-<p>
-<hr>
-<h2> Working with <i>Bacillus subtilis</i></h2>
+<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/1/17/T--TU_Dresden--M_Q5PCR.pdf">PCR with Q5 Polymerase</a>
-<a class="pdf-resources" target="_blank" href= "https://static.igem.org/mediawiki/2017/0/05/Dna_isolation_for_pcr.pdf">DNA isolation (for PCR)</a>
-<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/4/4c/Dna_isolation_for_transformation.pdf">DNA isolation (for transformation)</a>
+<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/b/bf/T--TU_Dresden--M_LFH.pdf">Long Flanking Homology PCR</a>
-<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/5/5f/Bacillus_transformation.pdf">Transformation of <i>B. subtilis </i></a>
+<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/d/db/SOP_protocols_for_cloning_with_the_Evaluation_Vector_and_the_Signal_Peptide_Toolbox.pdf">SOP Protocols for Cloning with the Evaluation Vector and the Signal Peptide Toolbox</a>
-<p>
-<hr>
-<h2>Media and Solutions </h2>
+<h2>Primer</h2>
-<a class="pdf-resources" target="_blank" href= "https://static.igem.org/mediawiki/2017/6/62/Media_antibiotics.pdf">Media, Solutions & Antibiotics</a>
+<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/0/0f/Primer_list.pdf">Primer List General</a>
-<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/2/29/Jensens_media.pdf">Jensen's Medium</a>
+<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/b/b8/Primer_list_signal_peptides.pdf">Primer List Signal Peptides</a>
-<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/0/03/Mcse.pdf">MCSE Medium</a>
+</div>
-<p>
-<hr>
-<h2>Primer</h2>
+<div class="contentbox">
+<h1 class="box-heading">Working with <i>Bacillus subtilis</i></h1>
+<a class="pdf-resources" target="_blank" href= "https://static.igem.org/mediawiki/2017/0/05/Dna_isolation_for_pcr.pdf">DNA Isolation (for PCR)</a>
-<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/0/0f/Primer_list.pdf">Primerlist General</a>
+<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/4/4c/Dna_isolation_for_transformation.pdf">DNA Isolation (for Transformation)</a>
-<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/b/b8/Primer_list_signal_peptides.pdf">Primerlist Signalpeptides</a>
+<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/5/5f/Bacillus_transformation.pdf">Transformation of <i>B. subtilis </i></a>
-<p>
+</div>
-<hr>
+<div class="contentbox">
+<h1 class="box-heading">Media and Solutions </h1>
+<a class="pdf-resources" target="_blank" href= "https://static.igem.org/mediawiki/2017/6/62/Media_antibiotics.pdf">Media, Solutions & Antibiotics</a>
+<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/2/29/Jensens_media.pdf">Jensen's Medium</a>
+<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/0/03/Mcse.pdf">MCSE Medium</a>
+</div>
+<div class="contentbox">
-<h2>Assays</h2>
+<h1 class="box-heading">Assays</h1>
 <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/c/c8/Disc_diffusion.pdf">Disc Diffussion Assay</a>
@@ Line 65: / Line 68: @@
 <a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/3/3b/Secretion_assay.pdf">Fluorescence Assay</a>
+</div>
-<hr>
+<div class="contentbox">
+<h1 class="box-heading">Protein Work</h1>
-<h2>Protein work</h2>
-<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/d/d9/Proteins.pdf">His-Tag purification with agarose beads</a>
-<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/f/fa/Laemmli_SDS-PAGE.pdf">Laemmli SDS-PAGE</a
-<hr>
-<h2> Peptidosome methods </h2>
-<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/8/87/Protocols_peptidosomes.pdf">Peptidosome creation</a>
-<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/f/f6/T--TU_Dresden--EncapsulationofBsubtilis.pdf">Encapsulation of <i>B. subtilis</i></a>
-<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/4/41/Wellscan.pdf">Plate reader Wellscan Peptidosomes</a>
+<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/d/d9/Proteins.pdf">His-Tag Purification with Agarose Beads</a>
+<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/f/fa/Laemmli_SDS-PAGE.pdf">Laemmli SDS-PAGE</a>
+</div>
 </main>

Difference between revisions of "Team:TU Dresden/Experiments"

Latest revision as of 17:13, 13 December 2017

Peptidosome Methods

Cloning and Primer Lists

Cloning

Primer

Working with Bacillus subtilis

Media and Solutions

Assays

Protein Work