Difference between revisions of "Team:TU Dresden/Experiments"

 
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<div class="contentbox">
 
<div class="contentbox">
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<h1 class="box-heading"> Protocols</h1>
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<h1 class="box-heading">Peptidosome Methods </h1>
  
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<h2> Cloning </h2>
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<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/8/87/Protocols_peptidosomes.pdf">Peptidosome Creation</a>
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<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/f/f6/T--TU_Dresden--EncapsulationofBsubtilis.pdf">Encapsulation of <i>B. subtilis</i></a>
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<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/4/41/Wellscan.pdf">Plate Reader Wellscan Peptidosomes</a>
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</div>
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<div class="contentbox">
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<h1 class="box-heading"> Cloning and Primer Lists</h1>
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<h2>Cloning</h2>
 
<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/d/dc/New_cloning.pdf">Cloning Procedure</a>
 
<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/d/dc/New_cloning.pdf">Cloning Procedure</a>
  
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<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/d/db/SOP_protocols_for_cloning_with_the_Evaluation_Vector_and_the_Signal_Peptide_Toolbox.pdf">SOP Protocols for Cloning with the Evaluation Vector and the Signal Peptide Toolbox</a>
 
<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/d/db/SOP_protocols_for_cloning_with_the_Evaluation_Vector_and_the_Signal_Peptide_Toolbox.pdf">SOP Protocols for Cloning with the Evaluation Vector and the Signal Peptide Toolbox</a>
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<p>
 
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<hr>
 
  
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<h2> Working with <i>Bacillus subtilis</i></h2>
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<h2>Primer</h2>
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<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/0/0f/Primer_list.pdf">Primer List General</a>
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<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/b/b8/Primer_list_signal_peptides.pdf">Primer List Signal Peptides</a>
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</div>
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<div class="contentbox">
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<h1 class="box-heading">Working with <i>Bacillus subtilis</i></h1>
 
<a class="pdf-resources" target="_blank" href= "https://static.igem.org/mediawiki/2017/0/05/Dna_isolation_for_pcr.pdf">DNA Isolation (for PCR)</a>
 
<a class="pdf-resources" target="_blank" href= "https://static.igem.org/mediawiki/2017/0/05/Dna_isolation_for_pcr.pdf">DNA Isolation (for PCR)</a>
  
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<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/5/5f/Bacillus_transformation.pdf">Transformation of <i>B. subtilis </i></a>
 
<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/5/5f/Bacillus_transformation.pdf">Transformation of <i>B. subtilis </i></a>
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<p>
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</div>
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<hr>
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<h2>Media and Solutions </h2>
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<div class="contentbox">
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<h1 class="box-heading">Media and Solutions </h1>
  
 
<a class="pdf-resources" target="_blank" href= "https://static.igem.org/mediawiki/2017/6/62/Media_antibiotics.pdf">Media, Solutions & Antibiotics</a>
 
<a class="pdf-resources" target="_blank" href= "https://static.igem.org/mediawiki/2017/6/62/Media_antibiotics.pdf">Media, Solutions & Antibiotics</a>
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<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/0/03/Mcse.pdf">MCSE Medium</a>
 
<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/0/03/Mcse.pdf">MCSE Medium</a>
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<p>
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</div>
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<hr>
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<h2>Primer</h2>
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<div class="contentbox">
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<h1 class="box-heading">Assays</h1>
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<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/0/0f/Primer_list.pdf">Primerlist General</a>
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<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/b/b8/Primer_list_signal_peptides.pdf">Primerlist Signalpeptides</a>
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<p>
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<hr>
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<h2>Assays</h2>
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<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/c/c8/Disc_diffusion.pdf">Disc Diffussion Assay</a>
 
<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/c/c8/Disc_diffusion.pdf">Disc Diffussion Assay</a>
  
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<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/3/3b/Secretion_assay.pdf">Fluorescence Assay</a>
 
<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/3/3b/Secretion_assay.pdf">Fluorescence Assay</a>
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</div>
  
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<hr>
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<div class="contentbox">
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<h1 class="box-heading">Protein Work</h1>
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<h2>Protein Work</h2>
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<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/d/d9/Proteins.pdf">His-Tag Purification with Agarose Beads</a>
 
<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/d/d9/Proteins.pdf">His-Tag Purification with Agarose Beads</a>
  
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<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/f/fa/Laemmli_SDS-PAGE.pdf">Laemmli SDS-PAGE</a
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<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/f/fa/Laemmli_SDS-PAGE.pdf">Laemmli SDS-PAGE</a>
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</div>
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<hr>
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<h2> Peptidosome Methods </h2>
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<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/8/87/Protocols_peptidosomes.pdf">Peptidosome Creation</a>
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<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/f/f6/T--TU_Dresden--EncapsulationofBsubtilis.pdf">Encapsulation of <i>B. subtilis</i></a>
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<a class="pdf-resources" target="_blank" href="https://static.igem.org/mediawiki/2017/4/41/Wellscan.pdf">Plate Reader Wellscan Peptidosomes</a>
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</main>
 
</main>

Latest revision as of 17:13, 13 December 2017