Difference between revisions of "Team:Heidelberg/Materials"

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{{#tag:html|<script>{{Heidelberg/title|Materials}}</script>}}
 
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{{Heidelberg/main|Notebook|Materials|https://static.igem.org/mediawiki/2017/5/50/T--Heidelberg--2017_notebook-bg.png|orange|
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{{Heidelberg/templateus/Contentsection|{{Heidelberg/accpanel|#F8991D|{{Heidelberg/accord|Antibiotics|{{Heidelberg/templateus/Tablebox||{{#tag:html|<table class="table table-bordered mdl-shadow--4dp" XSSCleaned="overflow-x: scroll !important">
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<thead style="background-color: #005493 !important;"> <tr>
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<th>Name</th> <th>Supplier</th> <th></th> </tr>
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</thead><tbody> <tr>
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<td>Carbenicillin dissodium salt</td> <td>Carl Roth</td> <td></td> </tr>
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<tr>
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<td>Amicillin sodium salt</td> <td>Carl Roth</td> <td></td> </tr>
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<tr>
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<td>Chloramphenicol</td> <td>Carl Roth</td> <td></td> </tr>
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<tr>
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<td>Kanamycin sulfate</td> <td>Sigma Aldrich</td> <td></td> </tr>
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<tr>
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<td>Tetracycline</td> <td>Sigma Aldrich</td> <td></td> </tr>
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<tr>
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<td>Spectinomycine</td> <td>Sigma Aldrich</td> <td></td> </tr>
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</tbody></table>
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}}|
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}}|table_id-0}}
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{{Heidelberg/accord|Enzymes|{{Heidelberg/templateus/Tablebox||{{#tag:html|<table class="table table-bordered mdl-shadow--4dp" XSSCleaned="overflow-x: scroll !important">
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<thead style="background-color: #005493 !important;"> <tr>
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<th>Name</th> <th>Supplier</th> <th></th> <th></th> <th></th> <th></th> <th></th> <th></th> <th></th> <th></th> <th></th> </tr>
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</thead><tbody> <tr>
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<td>BamI </td> <td>New England BioLabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
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<tr>
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<td>BamHI-HF</td> <td>New England BioLabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
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<tr>
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<td>BgIII </td> <td>New England BioLabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
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<tr>
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<td>BsaI-HF</td> <td>New England BioLabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
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<tr>
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<td>BsmBI </td> <td>New England BioLabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
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<tr>
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<td>DpnI </td> <td>New England BioLabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
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<tr>
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<td>EcoRI </td> <td>New England BioLabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
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<tr>
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<td>EcoRI-HF </td> <td>New England BioLabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
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<tr>
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<td>Esp3I</td> <td>Thermo Fisher Scientific</td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
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<tr>
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<td>HindIII-HF </td> <td>New England BioLabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
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<tr>
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<td>Hinfl, recombinant</td> <td>New England BioLabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
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<tr>
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<td>HpaII </td> <td>New England BioLabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
 +
<tr>
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<td>KpnI-HF </td> <td>New England BioLabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
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<tr>
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<td>MfeI-HF </td> <td>New England BioLabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
 +
<tr>
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<td>MlyI, recombinant</td> <td>New England BioLabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
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<tr>
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<td>NheI-HF </td> <td>New England BioLabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
 +
<tr>
 +
<td>NotI-HF </td> <td>New England BioLabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
 +
<tr>
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<td>PacI </td> <td>New England BioLabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
 +
<tr>
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<td>PstI-HF </td> <td>New England BioLabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
 +
<tr>
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<td>PvuI-HF </td> <td>New England BioLabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
 +
<tr>
 +
<td>SalI-HF </td> <td>New England BioLabs </td> <td></td> <td></td> <td></td> <td> </td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
 +
<tr>
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<td>Sau3AI</td> <td>New England BioLabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
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<tr>
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<td>SpeI-HF </td> <td>New England BioLabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
 +
<tr>
 +
<td>XbaI </td> <td>New England BioLabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
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</tbody></table>
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}}|
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}}|table_id-1}}
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{{Heidelberg/accord|Equipment|{{Heidelberg/templateus/Tablebox||{{#tag:html|<table class="table table-bordered mdl-shadow--4dp" XSSCleaned="overflow-x: scroll !important">
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<thead style="background-color: #005493 !important;"> <tr>
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<th>Equipment</th> <th>Name</th> <th>Manufacturer</th> <th></th> <th></th> </tr>
 +
</thead><tbody> <tr>
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<td></td> <td></td> <td></td> <td></td> <td></td> </tr>
 +
<tr>
 +
<td>Table Centrifuge </td> <td>Microfuge® 18 Centrifuge </td> <td>Beckman CoulterTM</td> <td></td> <td></td> </tr>
 +
<tr>
 +
<td>Table Centrifuge </td> <td>Microfuge® 22R Centrifuge </td> <td>Beckman CoulterTM</td> <td></td> <td></td> </tr>
 +
<tr>
 +
<td>Centrifuge </td> <td>Allegra X-12R </td> <td>Beckman CoulterTM</td> <td></td> <td></td> </tr>
 +
<tr>
 +
<td>Shaker </td> <td>VORTEXGENIE 2 </td> <td>Scientific Industries, SITM</td> <td></td> <td></td> </tr>
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<tr>
 +
<td>Heating plate (magnet stirrer)</td> <td>MR Hei-Standard </td> <td>Heidolph</td> <td></td> <td></td> </tr>
 +
<tr>
 +
<td>Heatblock </td> <td>QBD2</td> <td>Grant</td> <td></td> <td></td> </tr>
 +
<tr>
 +
<td>Heatblock </td> <td>QBD4 </td> <td>Grant</td> <td></td> <td></td> </tr>
 +
<tr>
 +
<td>Heatblock (shaking function) </td> <td>Thermomixer comfort </td> <td>Eppendorf</td> <td></td> <td></td> </tr>
 +
<tr>
 +
<td>PCR-Machine</td> <td>MyCycler thermo cycler</td> <td>BioRad</td> <td></td> <td></td> </tr>
 +
<tr>
 +
<td>PCR-Machine</td> <td>T100 Therma Cycler</td> <td>BioRad</td> <td></td> <td></td> </tr>
 +
<tr>
 +
<td>PCR-Machine</td> <td>Mastercycler nexus X2</td> <td>Eppendorf </td> <td></td> <td></td> </tr>
 +
<tr>
 +
<td>Gel documentation</td> <td>Transluminator </td> <td>Vilber Lourmat</td> <td></td> <td></td> </tr>
 +
<tr>
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<td>Scale (fine)</td> <td>PioneerTM PA114C </td> <td>OHAUS</td> <td></td> <td></td> </tr>
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<tr>
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<td>Scale</td> <td>PioneerTM PA4101C </td> <td>OHAUS</td> <td></td> <td></td> </tr>
 +
<tr>
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<td>Incubator</td> <td>KBW 400</td> <td>Binder</td> <td></td> <td></td> </tr>
 +
<tr>
 +
<td>Incubator/ Shaker</td> <td>ISF1-X</td> <td>Kuhner shaker</td> <td></td> <td></td> </tr>
 +
<tr>
 +
<td>Incubator / Shaker</td> <td>HT Multitron Version 2 </td> <td>INFORS</td> <td></td> <td></td> </tr>
 +
<tr>
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<td>Plate Reader </td> <td>Infinite M200 </td> <td>Tecan</td> <td></td> <td></td> </tr>
 +
<tr>
 +
<td>Plate Reader </td> <td>GloMax® Discover</td> <td>Promega</td> <td></td> <td></td> </tr>
 +
<tr>
 +
<td>UV/VIS Spectrometer </td> <td>Ultrospec 3300 pro </td> <td>Amersham Biosciences</td> <td></td> <td></td> </tr>
 +
<tr>
 +
<td>Microvulume spectrometer</td> <td>NanoPhotometer NP80</td> <td>Implen</td> <td></td> <td></td> </tr>
 +
<tr>
 +
<td>PH-Meter</td> <td>PH-Meter 765 Calimatic </td> <td>Knick</td> <td></td> <td></td> </tr>
 +
<tr>
 +
<td>Lyophilizer </td> <td>Gefriertrocknungsanlage ALPHA 1-2 LD Plus Best. Nr. 101521 </td> <td>Christ</td> <td></td> <td></td> </tr>
 +
<tr>
 +
<td>Sonificator</td> <td>Sonoplus Gm 2070 (2002)</td> <td>Bandelin</td> <td></td> <td></td> </tr>
 +
<tr>
 +
<td>Vacuum Manifold </td> <td>Qiavac 24 Plus</td> <td>Qiagen</td> <td></td> <td></td> </tr>
 +
<tr>
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<td>HPLC</td> <td>HPLC system with binary pump, VWD detector and autosampler</td> <td>Knauer</td> <td></td> <td></td> </tr>
 +
<tr>
 +
<td>Nucleic acid purification system</td> <td>QIAcube</td> <td>Qiagen</td> <td></td> <td></td> </tr>
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 +
</tbody></table>
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}}|
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}}|table_id-2}}
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{{Heidelberg/accord|Kits|{{Heidelberg/templateus/Tablebox||{{#tag:html|<table class="table table-bordered mdl-shadow--4dp" XSSCleaned="overflow-x: scroll !important">
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<thead style="background-color: #005493 !important;"> <tr>
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<th>Name</th> <th>Supplier</th> <th></th> <th></th> </tr>
 +
</thead><tbody> <tr>
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<td>QIAquick® Gel Extraction Kit</td> <td>Qiagen </td> <td></td> <td></td> </tr>
 +
<tr>
 +
<td>QIAquick® PCR Purification Kit </td> <td>Qiagen </td> <td></td> <td></td> </tr>
 +
<tr>
 +
<td>QIAprep® Spin Miniprep Kit </td> <td>Qiagen </td> <td></td> <td></td> </tr>
 +
<tr>
 +
<td>DNA Clean & Concentrator™-5</td> <td>Zymo Research</td> <td></td> <td></td> </tr>
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<tr>
 +
<td>ZymoPURE™ Plasmid Miniprep Kit</td> <td>Zymo Research</td> <td></td> <td></td> </tr>
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<tr>
 +
<td>ZymoPURE™ Plasmid Midiprep Kit</td> <td>Zymo Research</td> <td></td> <td></td> </tr>
 +
<tr>
 +
<td></td> <td></td> <td></td> <td></td> </tr>
 +
<tr>
 +
<td></td> <td></td> <td></td> <td></td> </tr>
 +
<tr>
 +
<td></td> <td></td> <td></td> <td></td> </tr>
 +
<tr>
 +
<td></td> <td></td> <td></td> <td></td> </tr>
 +
<tr>
 +
<td>NEB iGEM Support Kit 2</td> <td>New England Biolabs</td> <td></td> <td></td> </tr>
 +
<tr>
 +
<td>NEB Quick Cloning Box-HF</td> <td>New England Biolabs</td> <td></td> <td></td> </tr>
 +
<tr>
 +
<td>HiScribe T7 Quick High Yield RNA Synthesis Kit</td> <td>New England Biolabs</td> <td></td> <td></td> </tr>
 +
<tr>
 +
<td>MinION Basic starter pack (1 MinION, 2 Flow cells, 1 Sequencing Kit, 1 Wash kit)</td> <td>Oxford Nanopore </td> <td></td> <td></td> </tr>
 +
</tbody></table>
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}}|
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}}|table_id-3}}
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{{Heidelberg/accord|Markers|{{Heidelberg/templateus/Tablebox||{{#tag:html|<table class="table table-bordered mdl-shadow--4dp" XSSCleaned="overflow-x: scroll !important">
 +
<thead style="background-color: #005493 !important;"> <tr>
 +
<th>Name</th> <th>Supplier</th> <th></th> <th></th> </tr>
 +
</thead><tbody> <tr>
 +
<td>Quick-Load 2-Log DNA Ladder (0.1-10.0 kb) </td> <td>New England BioLabs </td> <td></td> <td></td> </tr>
 +
<tr>
 +
<td>Quick-Load 1 kb DNA Ladder </td> <td>New England BioLabs </td> <td></td> <td></td> </tr>
 +
<tr>
 +
<td>100 bp DNA Ladder</td> <td>New England BioLabs </td> <td></td> <td></td> </tr>
 +
<tr>
 +
<td>Novex® Sharp Pre-stained Protein Standard </td> <td>Invitrogen </td> <td></td> <td></td> </tr>
 +
<tr>
 +
<td>SERVA Triple Color Protein Standard </td> <td>SERVA Electrophoresis </td> <td></td> <td></td> </tr>
 +
</tbody></table>
 +
}}|
 +
}}|table_id-4}}
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{{Heidelberg/accord|Media|{{Heidelberg/templateus/Tablebox||{{#tag:html|<table class="table table-bordered mdl-shadow--4dp" XSSCleaned="overflow-x: scroll !important">
 +
<thead style="background-color: #005493 !important;"> <tr>
 +
<th>Media</th> <th>Composition</th> <th></th> </tr>
 +
</thead><tbody> <tr>
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<td>LB medium (Luria/Miller für d-2,5 kg Best. Nr.: X968.3</td> <td>10 g NaCl, 10 g tryptone/ peptone, 5 g yeast extract in 1 L dH2O</td> <td></td> </tr>
 +
<tr>
 +
<td>2xYT medium für die Molekularb-2,5 kg Best. Nr.: X966.3</td> <td>5 g NaCl, 16 g tryptone/ peptone, 10 g yeast extract in 1 L dH2O</td> <td></td> </tr>
 +
<tr>
 +
<td>SOB medium</td> <td>0,5 % yeast extract, 2 % tryptone/peptone, 10 mM NaCl, 2.5 mM KCl, 10 mM MgCl2, 10 mM MgSO4</td> <td></td> </tr>
 +
<tr>
 +
<td>SOB medium</td> <td>SOB supplemented with 20 mM glucose</td> <td></td> </tr>
 +
<tr>
 +
<td>M9 minimal medium</td> <td></td> <td></td> </tr>
 +
</tbody></table>
 +
}}|
 +
}}|table_id-5}}
 +
{{Heidelberg/accord|Other Enzymes|{{Heidelberg/templateus/Tablebox||{{#tag:html|<table class="table table-bordered mdl-shadow--4dp" XSSCleaned="overflow-x: scroll !important">
 +
<thead style="background-color: #005493 !important;"> <tr>
 +
<th>Name</th> <th>Supplier</th> <th></th> <th></th> <th></th> <th></th> <th></th> <th></th> </tr>
 +
</thead><tbody> <tr>
 +
<td>Gibson Assembly® Master Mix </td> <td>New England Biolabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
 +
<tr>
 +
<td>T4 DNA Ligase </td> <td>New England Biolabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
 +
<tr>
 +
<td>T7 RNA Polymerase</td> <td>New England Biolabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
 +
<tr>
 +
<td>T4 Polynucleotide Kinase (PNK)</td> <td>New England Biolabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
 +
<tr>
 +
<td>T4 RNA Ligase 1 (ssRNA Ligase)</td> <td>New England Biolabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
 +
<tr>
 +
<td>Alkaline Phosphatase (CIP)</td> <td>New England Biolabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
 +
<tr>
 +
<td>Shrimp Alkaline Phosphatase (rSAP)</td> <td>New England Biolabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
 +
</tbody></table>
 +
}}|
 +
}}|table_id-6}}
 +
{{Heidelberg/accord|PACE|{{Heidelberg/templateus/Tablebox||{{#tag:html|<table class="table table-bordered mdl-shadow--4dp" XSSCleaned="overflow-x: scroll !important">
 +
<thead><tr class="tableizer-firstrow"><th>Product </th><th>Source </th><th>Catalog # </th></tr></thead><tbody>
 +
<tr><td>&nbsp;</td><td>&nbsp;</td><td>&nbsp;</td></tr>
 +
<tr><td>Turbidostat</td><td>&nbsp;</td><td>&nbsp;</td></tr>
 +
<tr><td>Bioreactor 2L MyFerm III </td><td>HLL</td><td>L14023002</td></tr>
 +
<tr><td>Screw caps with borehole GL45</td><td>Carl Roth </td><td>L994.1</td></tr>
 +
<tr><td>Septa for screw caps with borehole GL45</td><td>Carl Roth </td><td>LL52.1</td></tr>
 +
<tr><td>Septa for screw caps with borehole GL32</td><td>Carl Roth </td><td>LL51.1</td></tr>
 +
<tr><td>Screw caps with borehole GL32</td><td>Carl Roth </td><td>H396.1</td></tr>
 +
<tr><td>Tubing,pharmed,2.79mm ID, 100´</td><td>Cole Parmer, Inc</td><td>95809-48</td></tr>
 +
<tr><td>Male luer with lock ring x 1/8" hose barb, PP, 25/pk</td><td>Cole Parmer, Inc</td><td>45503-04</td></tr>
 +
<tr><td>Female luer x 1/8" hose barb adapter, PP, 25/pk</td><td>Cole Parmer, Inc</td><td>45500-04</td></tr>
 +
<tr><td>Screw caps with borehole GL18</td><td>Carl Roth </td><td>EL61.1</td></tr>
 +
<tr><td>Septa for screw caps with borehole GL18</td><td>Carl Roth </td><td>LL49.1</td></tr>
 +
<tr><td>GL 18 pressure equaliser set</td><td>Carl Roth </td><td>KE58.1</td></tr>
 +
<tr><td>GL 18 screw cap</td><td>Carl Roth </td><td>E696.1</td></tr>
 +
<tr><td>Lab screw fittings 3,2 mm outer diameter</td><td>Carl Roth </td><td>KE53.1</td></tr>
 +
<tr><td>Bottle multi-dispensers, made of PP</td><td>Carl Roth </td><td>HX85.1</td></tr>
 +
<tr><td>Rotilabo®-syringe filters, PTFE, sterile, Ø 25 mm, 0.2 µm</td><td>Carl Roth </td><td>KC76.1</td></tr>
 +
<tr><td>&nbsp;</td><td>&nbsp;</td><td>&nbsp;</td></tr>
 +
<tr><td>Lagoons</td><td>&nbsp;</td><td>&nbsp;</td></tr>
 +
<tr><td>Fisherbrand™ Reusable Glass Media Bottles with Cap 250 ml </td><td>Fisherscientific</td><td>15456113</td></tr>
 +
<tr><td>Septa for screw caps with borehole GL45</td><td>Carl Roth </td><td>LL52.1</td></tr>
 +
<tr><td>Screw caps with borehole GL45</td><td>Carl Roth </td><td>L994.1</td></tr>
 +
<tr><td>BD 23G 1 in. - 0.6 mm x 25 mm</td><td>BD</td><td>305892</td></tr>
 +
<tr><td>Rotilabo®-syringe filters, PTFE, sterile, Ø 25 mm, 0.2 µm</td><td>Carl Roth </td><td>KC76.1</td></tr>
 +
<tr><td>Tubing,pharmed,0.89mm ID, 100ft</td><td>Cole Parmer, Inc</td><td>95809-26</td></tr>
 +
<tr><td>Screw caps with borehole GL14</td><td>Carl Roth </td><td>L993.1</td></tr>
 +
<tr><td>Septa for screw caps with borehole GL14</td><td>Carl Roth </td><td>LL48.1</td></tr>
 +
<tr><td>GL 14 pressure equaliser set</td><td>Carl Roth </td><td>HX90.1</td></tr>
 +
<tr><td>GL 14 screw cap</td><td>Carl Roth </td><td>E695.1</td></tr>
 +
<tr><td>GL 14 laboratory screw joint for hose with 1,6 outer diameter</td><td>Carl Roth </td><td>HX86.1</td></tr>
 +
<tr><td>GL 14 laboratory screw joint for hose with 3,0 mm outer diameter</td><td>Carl Roth </td><td>HX87.1</td></tr>
 +
<tr><td>&nbsp;</td><td>&nbsp;</td><td>&nbsp;</td></tr>
 +
<tr><td>Waste</td><td>&nbsp;</td><td>&nbsp;</td></tr>
 +
<tr><td>Flaschen mit Quetschhahn 20 lt- Weich-Polyethylen (PE-LD), viereckig</td><td>Semadeni Plastic Group</td><td>1035</td></tr>
 +
<tr><td>BD 23G 1 in. - 0.6 mm x 25 mm</td><td>BD</td><td>305892</td></tr>
 +
<tr><td>&nbsp;</td><td>&nbsp;</td><td>&nbsp;</td></tr>
 +
<tr><td>Peristaltic pump (lagoon line)</td><td>&nbsp;</td><td>&nbsp;</td></tr>
 +
<tr><td>L/S brushless programmable drive </td><td>Cole Parmer, Inc</td><td>07550-50</td></tr>
 +
<tr><td>L/S 8-channel pump head for microbore tubing</td><td>Cole Parmer, Inc</td><td>07534-08</td></tr>
 +
<tr><td>&nbsp;</td><td>&nbsp;</td><td>&nbsp;</td></tr>
 +
<tr><td>Peristaltic pump (media line)</td><td>&nbsp;</td><td>&nbsp;</td></tr>
 +
<tr><td>Masterflex L/S economy variable drive </td><td>Cole Parmer, Inc</td><td>07554-80</td></tr>
 +
<tr><td>L/S 8-channel, 3-roller pump head</td><td>Cole Parmer, Inc</td><td>07519-05</td></tr>
 +
<tr><td>Masterflex L/S small catridges</td><td>Cole Parmer, Inc</td><td>07519-80</td></tr>
 +
<tr><td>&nbsp;</td><td>&nbsp;</td><td>&nbsp;</td></tr>
 +
<tr><td>Esvelt medium </td><td>&nbsp;</td><td>&nbsp;</td></tr>
 +
<tr><td>Carboys, made of PP 20 L </td><td>Carl Roth</td><td>C480.1</td></tr>
 +
<tr><td>&nbsp;</td><td>&nbsp;</td><td>&nbsp;</td></tr>
 +
<tr><td>valve control </td><td>&nbsp;</td><td>&nbsp;</td></tr>
 +
<tr><td>3-way valve</td><td>Conrad</td><td>100P3MP24-05S </td></tr>
 +
<tr><td>&nbsp;</td><td>&nbsp;</td><td>&nbsp;</td></tr>
 +
<tr><td>syringe pump </td><td>&nbsp;</td><td>&nbsp;</td></tr>
 +
<tr><td>Tubing,pharmed,0.89mm ID, 100ft</td><td>Cole Parmer, Inc</td><td>95809-26</td></tr>
 +
<tr><td>Microbore two-stop tube sets, silicone; 0.89 mm ID</td><td>Cole Parmer, Inc</td><td>GZ-06421-26</td></tr>
 +
<tr><td>BD 23G 1 in. - 0.6 mm x 25 mm</td><td>BD</td><td>305892</td></tr>
 +
<tr><td>Perfusor® compact</td><td>Bbraun</td><td>8714827</td></tr>
 +
<tr><td>&nbsp;</td><td>&nbsp;</td><td>&nbsp;</td></tr>
 +
<tr><td>media line</td><td>&nbsp;</td><td>&nbsp;</td></tr>
 +
<tr><td>Microbore two-stop tube sets, silicone; 2.06 mm ID</td><td>Cole Parmer, Inc</td><td>06421-42</td></tr>
 +
<tr><td>Tubing,pharmed,2.06mm ID, 100ft</td><td>Cole Parmer, Inc</td><td>95809-42</td></tr>
 +
<tr><td>Female luer x 1/16" hose barb adapter, PP, 25/pk </td><td>Cole Parmer, Inc</td><td>45500-00</td></tr>
 +
<tr><td>Male luer with lock ring x 1/16" hose barb, PP, 25/pk</td><td>Cole Parmer, Inc</td><td>45503-00</td></tr>
 +
<tr><td>TuFlux® SIL - TUBE 1/8 ID X 1/4 OD</td><td>Sartorius</td><td>FSA117425</td></tr>
 +
<tr><td>&nbsp;</td><td>&nbsp;</td><td>&nbsp;</td></tr>
 +
<tr><td>lagoon line</td><td>&nbsp;</td><td>&nbsp;</td></tr>
 +
<tr><td>Microbore two-stop tube sets, silicone; 1.42 mm ID</td><td>Cole Parmer, Inc</td><td>06421-34</td></tr>
 +
<tr><td>Male luer with lock ring x 3/32" hose barb, PP, 25/pk</td><td>Cole Parmer, Inc</td><td>45503-02</td></tr>
 +
<tr><td>Female luer x 3/32" hose barb adapter, PP, 25/pk</td><td>Cole Parmer, Inc</td><td>45500-02</td></tr>
 +
<tr><td>Tubing,pharmed,1.42mm ID, 100ft</td><td>Cole Parmer, Inc</td><td>SI-06460-34</td></tr>
 +
<tr><td>&nbsp;</td><td>&nbsp;</td><td>&nbsp;</td></tr>
 +
<tr><td>O2 line </td><td>&nbsp;</td><td>&nbsp;</td></tr>
 +
<tr><td>Sartopore® Air Midisart®</td><td>Sartorius</td><td>17805--------BVN</td></tr>
 +
<tr><td>&nbsp;</td><td>&nbsp;</td><td>&nbsp;</td></tr>
 +
<tr><td>ON-Line measurement</td><td>&nbsp;</td><td>&nbsp;</td></tr>
 +
<tr><td>5 m Sensor Cable </td><td>Hamilton</td><td>243870</td></tr>
 +
<tr><td>Incyte Pre-Amp</td><td>Hamilton</td><td>243720</td></tr>
 +
<tr><td>Cell Density ComBox</td><td>Hamilton</td><td>243810</td></tr>
 +
<tr><td>Incyte sensor DN12 - 220 mm </td><td>Hamilton</td><td>243733</td></tr>
  
 +
</tbody></table>
 +
}}|
 +
}}|table_id-100}}
  
 +
{{Heidelberg/accord|Polymerases|{{Heidelberg/templateus/Tablebox||{{#tag:html|<table class="table table-bordered mdl-shadow--4dp" XSSCleaned="overflow-x: scroll !important">
 +
<thead style="background-color: #005493 !important;"> <tr>
 +
<th>Name</th> <th>Supplier</th> <th></th> <th></th> <th></th> <th></th> <th></th> <th></th> </tr>
 +
</thead><tbody> <tr>
 +
<td>OneTaq® Quick-Load® 2X Master Mix with Standard Buffer</td> <td>New England Biolabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
 +
<tr>
 +
<td>Phusion Flash High-Fidelity PCR Master Mix</td> <td>Thermo Fisher Scientific</td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
 +
<tr>
 +
<td>Q5® High-Fidelity DNA Polymerase 2X Master Mix</td> <td>New England Biolabs </td> <td></td> <td></td> <td></td> <td></td> <td></td> <td></td> </tr>
 +
</tbody></table>
 +
}}|
 +
}}|table_id-7}}
  
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{{Heidelberg/footer}}
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{{Heidelberg/accord|Strains and Phages|{{#tag:html|<a href="https://2017.igem.org/Team:Heidelberg/Database">Visit our Notebook Database and have a look at the Strains section</a>
 +
}}|table_id-8}}
 +
 
 +
{{Heidelberg/accord|Plasmids|{{#tag:html|<a href="https://2017.igem.org/Team:Heidelberg/Database">Visit our Notebook Database and have a look at the Plasmids section</a>
 +
}}|table_id-9}}
 +
 
 +
 
 +
{{Heidelberg/accord|Primers and Oligonucleotides|{{#tag:html|<a href="https://2017.igem.org/Team:Heidelberg/Database">Visit our Notebook Database and have a look at the Primers section</a>
 +
}}|table_id-10}}
 +
 
 +
}}
 +
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 +
}}{{Heidelberg/footer}}

Latest revision as of 23:59, 15 December 2017

Notebook
Materials
Antibiotics

Name Supplier
Carbenicillin dissodium salt Carl Roth
Amicillin sodium salt Carl Roth
Chloramphenicol Carl Roth
Kanamycin sulfate Sigma Aldrich
Tetracycline Sigma Aldrich
Spectinomycine Sigma Aldrich
Enzymes

Name Supplier
BamI  New England BioLabs 
BamHI-HF New England BioLabs 
BgIII  New England BioLabs 
BsaI-HF New England BioLabs 
BsmBI  New England BioLabs 
DpnI  New England BioLabs 
EcoRI  New England BioLabs 
EcoRI-HF  New England BioLabs 
Esp3I Thermo Fisher Scientific
HindIII-HF  New England BioLabs 
Hinfl, recombinant New England BioLabs 
HpaII  New England BioLabs 
KpnI-HF  New England BioLabs 
MfeI-HF  New England BioLabs 
MlyI, recombinant New England BioLabs 
NheI-HF  New England BioLabs 
NotI-HF  New England BioLabs 
PacI  New England BioLabs 
PstI-HF  New England BioLabs 
PvuI-HF  New England BioLabs 
SalI-HF  New England BioLabs 
Sau3AI New England BioLabs 
SpeI-HF  New England BioLabs 
XbaI  New England BioLabs 
Equipment

Equipment Name Manufacturer
Table Centrifuge  Microfuge® 18 Centrifuge  Beckman CoulterTM
Table Centrifuge  Microfuge® 22R Centrifuge  Beckman CoulterTM
Centrifuge  Allegra X-12R  Beckman CoulterTM
Shaker  VORTEXGENIE 2  Scientific Industries, SITM
Heating plate (magnet stirrer) MR Hei-Standard  Heidolph
Heatblock  QBD2 Grant
Heatblock  QBD4  Grant
Heatblock (shaking function)  Thermomixer comfort  Eppendorf
PCR-Machine MyCycler thermo cycler BioRad
PCR-Machine T100 Therma Cycler BioRad
PCR-Machine Mastercycler nexus X2 Eppendorf
Gel documentation Transluminator  Vilber Lourmat
Scale (fine) PioneerTM PA114C  OHAUS
Scale PioneerTM PA4101C  OHAUS
Incubator KBW 400 Binder
Incubator/ Shaker ISF1-X Kuhner shaker
Incubator / Shaker HT Multitron Version 2  INFORS
Plate Reader  Infinite M200  Tecan
Plate Reader  GloMax® Discover Promega
UV/VIS Spectrometer  Ultrospec 3300 pro  Amersham Biosciences
Microvulume spectrometer NanoPhotometer NP80 Implen
PH-Meter PH-Meter 765 Calimatic  Knick
Lyophilizer  Gefriertrocknungsanlage ALPHA 1-2 LD Plus Best. Nr. 101521  Christ
Sonificator Sonoplus Gm 2070 (2002) Bandelin
Vacuum Manifold  Qiavac 24 Plus Qiagen
HPLC HPLC system with binary pump, VWD detector and autosampler Knauer
Nucleic acid purification system QIAcube Qiagen
Kits

Name Supplier
QIAquick® Gel Extraction Kit Qiagen 
QIAquick® PCR Purification Kit Qiagen 
QIAprep® Spin Miniprep Kit Qiagen 
DNA Clean & Concentrator™-5 Zymo Research
ZymoPURE™ Plasmid Miniprep Kit Zymo Research
ZymoPURE™ Plasmid Midiprep Kit Zymo Research
NEB iGEM Support Kit 2 New England Biolabs
NEB Quick Cloning Box-HF New England Biolabs
HiScribe T7 Quick High Yield RNA Synthesis Kit New England Biolabs
MinION Basic starter pack (1 MinION, 2 Flow cells, 1 Sequencing Kit, 1 Wash kit) Oxford Nanopore
Markers

Name Supplier
Quick-Load 2-Log DNA Ladder (0.1-10.0 kb)  New England BioLabs 
Quick-Load 1 kb DNA Ladder  New England BioLabs 
100 bp DNA Ladder New England BioLabs 
Novex® Sharp Pre-stained Protein Standard  Invitrogen 
SERVA Triple Color Protein Standard  SERVA Electrophoresis 
Media

Media Composition
LB medium (Luria/Miller für d-2,5 kg Best. Nr.: X968.3 10 g NaCl, 10 g tryptone/ peptone, 5 g yeast extract in 1 L dH2O
2xYT medium für die Molekularb-2,5 kg Best. Nr.: X966.3 5 g NaCl, 16 g tryptone/ peptone, 10 g yeast extract in 1 L dH2O
SOB medium 0,5 % yeast extract, 2 % tryptone/peptone, 10 mM NaCl, 2.5 mM KCl, 10 mM MgCl2, 10 mM MgSO4
SOB medium SOB supplemented with 20 mM glucose
M9 minimal medium
Other Enzymes

Name Supplier
Gibson Assembly® Master Mix  New England Biolabs 
T4 DNA Ligase  New England Biolabs
T7 RNA Polymerase New England Biolabs
T4 Polynucleotide Kinase (PNK) New England Biolabs
T4 RNA Ligase 1 (ssRNA Ligase) New England Biolabs
Alkaline Phosphatase (CIP) New England Biolabs
Shrimp Alkaline Phosphatase (rSAP) New England Biolabs
PACE

Product Source Catalog #
   
Turbidostat  
Bioreactor 2L MyFerm III HLLL14023002
Screw caps with borehole GL45Carl Roth L994.1
Septa for screw caps with borehole GL45Carl Roth LL52.1
Septa for screw caps with borehole GL32Carl Roth LL51.1
Screw caps with borehole GL32Carl Roth H396.1
Tubing,pharmed,2.79mm ID, 100´Cole Parmer, Inc95809-48
Male luer with lock ring x 1/8" hose barb, PP, 25/pkCole Parmer, Inc45503-04
Female luer x 1/8" hose barb adapter, PP, 25/pkCole Parmer, Inc45500-04
Screw caps with borehole GL18Carl Roth EL61.1
Septa for screw caps with borehole GL18Carl Roth LL49.1
GL 18 pressure equaliser setCarl Roth KE58.1
GL 18 screw capCarl Roth E696.1
Lab screw fittings 3,2 mm outer diameterCarl Roth KE53.1
Bottle multi-dispensers, made of PPCarl Roth HX85.1
Rotilabo®-syringe filters, PTFE, sterile, Ø 25 mm, 0.2 µmCarl Roth KC76.1
   
Lagoons  
Fisherbrand™ Reusable Glass Media Bottles with Cap 250 ml Fisherscientific15456113
Septa for screw caps with borehole GL45Carl Roth LL52.1
Screw caps with borehole GL45Carl Roth L994.1
BD 23G 1 in. - 0.6 mm x 25 mmBD305892
Rotilabo®-syringe filters, PTFE, sterile, Ø 25 mm, 0.2 µmCarl Roth KC76.1
Tubing,pharmed,0.89mm ID, 100ftCole Parmer, Inc95809-26
Screw caps with borehole GL14Carl Roth L993.1
Septa for screw caps with borehole GL14Carl Roth LL48.1
GL 14 pressure equaliser setCarl Roth HX90.1
GL 14 screw capCarl Roth E695.1
GL 14 laboratory screw joint for hose with 1,6 outer diameterCarl Roth HX86.1
GL 14 laboratory screw joint for hose with 3,0 mm outer diameterCarl Roth HX87.1
   
Waste  
Flaschen mit Quetschhahn 20 lt- Weich-Polyethylen (PE-LD), viereckigSemadeni Plastic Group1035
BD 23G 1 in. - 0.6 mm x 25 mmBD305892
   
Peristaltic pump (lagoon line)  
L/S brushless programmable drive Cole Parmer, Inc07550-50
L/S 8-channel pump head for microbore tubingCole Parmer, Inc07534-08
   
Peristaltic pump (media line)  
Masterflex L/S economy variable drive Cole Parmer, Inc07554-80
L/S 8-channel, 3-roller pump headCole Parmer, Inc07519-05
Masterflex L/S small catridgesCole Parmer, Inc07519-80
   
Esvelt medium   
Carboys, made of PP 20 L Carl RothC480.1
   
valve control   
3-way valveConrad100P3MP24-05S
   
syringe pump   
Tubing,pharmed,0.89mm ID, 100ftCole Parmer, Inc95809-26
Microbore two-stop tube sets, silicone; 0.89 mm IDCole Parmer, IncGZ-06421-26
BD 23G 1 in. - 0.6 mm x 25 mmBD305892
Perfusor® compactBbraun8714827
   
media line  
Microbore two-stop tube sets, silicone; 2.06 mm IDCole Parmer, Inc06421-42
Tubing,pharmed,2.06mm ID, 100ftCole Parmer, Inc95809-42
Female luer x 1/16" hose barb adapter, PP, 25/pk Cole Parmer, Inc45500-00
Male luer with lock ring x 1/16" hose barb, PP, 25/pkCole Parmer, Inc45503-00
TuFlux® SIL - TUBE 1/8 ID X 1/4 ODSartoriusFSA117425
   
lagoon line  
Microbore two-stop tube sets, silicone; 1.42 mm IDCole Parmer, Inc06421-34
Male luer with lock ring x 3/32" hose barb, PP, 25/pkCole Parmer, Inc45503-02
Female luer x 3/32" hose barb adapter, PP, 25/pkCole Parmer, Inc45500-02
Tubing,pharmed,1.42mm ID, 100ftCole Parmer, IncSI-06460-34
   
O2 line   
Sartopore® Air Midisart®Sartorius17805--------BVN
   
ON-Line measurement  
5 m Sensor Cable Hamilton243870
Incyte Pre-AmpHamilton243720
Cell Density ComBoxHamilton243810
Incyte sensor DN12 - 220 mm Hamilton243733
Polymerases

Name Supplier
OneTaq® Quick-Load® 2X Master Mix with Standard Buffer New England Biolabs 
Phusion Flash High-Fidelity PCR Master Mix Thermo Fisher Scientific
Q5® High-Fidelity DNA Polymerase 2X Master Mix New England Biolabs 
Strains and Phages
Plasmids
Primers and Oligonucleotides