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We also amplify B series plasmid in miniMos system for microinjection. | We also amplify B series plasmid in miniMos system for microinjection. | ||
− | We integrated ord-10:: | + | We integrated ord-10::CoChR::GEM-GECO::mCherry and str-1::Chrimson::GEM-GECO::GFP fusion genes into C.elegans(Caenorhabditis elegans) by microinjection respectively. |
{{SUSTech_Image_Center_8 | filename=T--SUSTech_Shenzhen--Results-plasmid1.png|width=800px|caption=<B>ord-10::CoCHR::GEM-geco::mCherry</B>}} | {{SUSTech_Image_Center_8 | filename=T--SUSTech_Shenzhen--Results-plasmid1.png|width=800px|caption=<B>ord-10::CoCHR::GEM-geco::mCherry</B>}} |
Revision as of 07:25, 31 October 2017
Results
Project
Contents
Optical Experiments Resutls
Under construction
| | First | Second | |--|------|---------| | 3 | 43 | 4 |
Microfluidic Experiments
Here, we fixed the Caenorhabditis elegans in immobilization chip to observe the Odr10::CoChR::GEM-GECO::mCherry worms under the fluorescence microscope and saw the neuronal activity successfully, which can confirm that the worms can express our target genes. We also put the Odr10::CoChR::GEM-GECO::mCherry worms into the chip, after a few minutes the worms would be inactive, then we can "wake up" the worms by the blue light.
Then, we demonstrated that the insertion did not damage the olfactory receptor neuron pairs of the worms by testing their response to diacetyl and 2-nonanone in Gaussian Plate.
Behavioral Experiments
Here, we confirmed that the Odr10::CoChR::GEM-GECO::mCherry worms could sense the blue light by inducing the Odr10::CoChR::GEM-GECO::mCherry worms to crawl a cycle on NGM plate. The Odr10::CoChR::GEM-GECO::mCherry worms could follow the blue light spot just like the attract of the food.
Then, in order to study the worms' learning ability we put the worms in alcohol layer on the NGM plate and stimulated them by the blue light at the same time. After 2 hours' training we found that the worms could crawl towards to the alcohol.
Plasmid Construction Results
According to the design of plasmid construction, we constructed ord-10::CoCHR::GEM-geco::mCherry and str-1::Chrimson::GEM-GECO::GFP fusion genes in backbone pCFJ909 successfully. The fusion gene segments were all be sequenced.
We also amplify B series plasmid in miniMos system for microinjection. We integrated ord-10::CoChR::GEM-GECO::mCherry and str-1::Chrimson::GEM-GECO::GFP fusion genes into C.elegans(Caenorhabditis elegans) by microinjection respectively.