Difference between revisions of "Team:ZJUT-China/Description"
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<h5>What should this page contain?</h5> | <h5>What should this page contain?</h5> | ||
<ul> | <ul> | ||
| − | <li> A | + | <li> A brief description of our project.</li> |
<p> | <p> | ||
We designed a new kind of genetically engineered E.coli which has the function of light-induced self-lysis or self-flocculation. To achieve this goal, we found two light controlled switches that can work in E.coli, one self-lysis gene named lysis and one self-flocculation gene named bcsB now. We plan to put one light reaction system and one effect gene into the same E.coli cell so that it can react to red or blue light with certain strength and then begin to self-lyse or self-flocculate. And if time permits, we’ll try to put two switches and two functional genes in the same one E.coli cell and test it to see if it could achieve different functions as the reactions to different colors of light. | We designed a new kind of genetically engineered E.coli which has the function of light-induced self-lysis or self-flocculation. To achieve this goal, we found two light controlled switches that can work in E.coli, one self-lysis gene named lysis and one self-flocculation gene named bcsB now. We plan to put one light reaction system and one effect gene into the same E.coli cell so that it can react to red or blue light with certain strength and then begin to self-lyse or self-flocculate. And if time permits, we’ll try to put two switches and two functional genes in the same one E.coli cell and test it to see if it could achieve different functions as the reactions to different colors of light. | ||
<p> | <p> | ||
| − | <li> | + | <li>Why do we chose to work on this genetically engineered E.coli at first?</li> |
<p> | <p> | ||
The solid-liquid separation and cell disruption are two major challenges in pharmaceutical industry. Those expensive equipments and toxic chemicals they need are the most annoying parts for fermentation factories. To solve these, we students came out of an idea that if we can provide some kind of E.coli which can be broken or flocculate in a cheap and controllable condition. After searching the literature, we found that light-induced switch system perfectly meets the qualification. The self-lysis gene and the self-flocculation gene had been found during that time. | The solid-liquid separation and cell disruption are two major challenges in pharmaceutical industry. Those expensive equipments and toxic chemicals they need are the most annoying parts for fermentation factories. To solve these, we students came out of an idea that if we can provide some kind of E.coli which can be broken or flocculate in a cheap and controllable condition. After searching the literature, we found that light-induced switch system perfectly meets the qualification. The self-lysis gene and the self-flocculation gene had been found during that time. | ||
<p> | <p> | ||
| − | <li>References and sources | + | <li>References and sources we used in our research.</li> |
<p> | <p> | ||
①Jeffrey J. Tabor,Anselm Levskaya,and Christopher A. Voigt. Multichromatic control of gene expression in Escherichia coli.J Mol Biol. 2011 January 14; 405(2): 315–324. | ①Jeffrey J. Tabor,Anselm Levskaya,and Christopher A. Voigt. Multichromatic control of gene expression in Escherichia coli.J Mol Biol. 2011 January 14; 405(2): 315–324. | ||
Revision as of 17:25, 29 June 2017
ZJUT-China
Description
Tell us about your project, describe what moves you and why this is something important for your team.
What should this page contain?
- A brief description of our project.
- Why do we chose to work on this genetically engineered E.coli at first?
- References and sources we used in our research.
- Use illustrations and other visual resources to explain your project.
We designed a new kind of genetically engineered E.coli which has the function of light-induced self-lysis or self-flocculation. To achieve this goal, we found two light controlled switches that can work in E.coli, one self-lysis gene named lysis and one self-flocculation gene named bcsB now. We plan to put one light reaction system and one effect gene into the same E.coli cell so that it can react to red or blue light with certain strength and then begin to self-lyse or self-flocculate. And if time permits, we’ll try to put two switches and two functional genes in the same one E.coli cell and test it to see if it could achieve different functions as the reactions to different colors of light.
The solid-liquid separation and cell disruption are two major challenges in pharmaceutical industry. Those expensive equipments and toxic chemicals they need are the most annoying parts for fermentation factories. To solve these, we students came out of an idea that if we can provide some kind of E.coli which can be broken or flocculate in a cheap and controllable condition. After searching the literature, we found that light-induced switch system perfectly meets the qualification. The self-lysis gene and the self-flocculation gene had been found during that time.
①Jeffrey J. Tabor,Anselm Levskaya,and Christopher A. Voigt. Multichromatic control of gene expression in Escherichia coli.J Mol Biol. 2011 January 14; 405(2): 315–324.
②Wu H, Wang Y, Wang Y, Cao X, Wu Y, Meng Z, Su Q, Wang Z, Yang S, Xu W, Liu S, Cheng P, Wu J, Khan MR, He L, Ma G.Quantitatively relating gene expression to light intensity via the serial connection of blue light sensor and CRISPRi.ACS Synth Biol. 2014 Dec 19;3(12):979-82. doi: 10.1021/sb500059x.
③Yoshihiro Ojima, Minh Hong Nguyen, Reiki Yajima, and Masahito Taya.Flocculation of Escherichia coli Cells in Association with Enhanced Production of Outer Membrane Vesicles. Appl Environ Microbiol. 2015 Sep 1;81(17):5900-6. doi: 10.1128/AEM.01011-15. Epub 2015 Jun 19.
④Laura Grande, Valeria Michelacci, Rosangela Tozzoli, Paola Ranieri, Antonella Maugliani, Alfredo Caprioli, and Stefano Morabito. Whole genome sequence comparison of vtx2-converting phages from Enteroaggregative Haemorrhagic Escherichia coli strains. BMC Genomics. 2014; 15(1): 574.Published online 2014 Jul 8. doi: 10.1186/1471-2164-15-574.
Advice on writing your Project Description
We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be consist, accurate and unambiguous in your achievements.
Judges like to read your wiki and know exactly what you have achieved. This is how you should think about these sections; from the point of view of the judge evaluating you at the end of the year.
References
iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.
Inspiration
See how other teams have described and presented their projects:
