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− | <style>
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| <html> | | <html> |
− | | + | <center> |
− | | + | <div style='padding-top: 40px;padding-bottom: 40px; font-size: 22px; font-family: "Optima";'>This part is designed to easily facilitate appending the protein degradation tag (pdt)#3 tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.</div> |
− | <style type="text/css">
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− |
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− | | + | |
− | #sideMenu, #top_title {display:none;}
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− | #bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 { margin-bottom: 0px; }
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− | | + | |
− | | + | |
− | | + | |
− | #page {
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− | width : 80%;
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− |
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− | margin-left : 10%;
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− |
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− | | + | |
− | }
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− | | + | |
− | | + | |
− | div.exp {font:normal 0.9em/1.5em; color:#A9A9A9;}
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− | a.exp {display:block;margin: 1em 0 0 0;text-decoration:none;border:0px solid black;border-radius:3px;background:#dce4ec;padding:10px;}
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− | a.exp::after {content:"»";float:right;}
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− | a.exp:focus {border-width: 1px 1px 0 1px;border-radius:4px 4px 0 0}
| + | |
− | a.exp + div {display:none;}
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− | a.exp:focus + div {display:block;border-width: 0 1px 1px 1px;border-style:solid; border-radius:0 0 4px 4px;border-color:black;}
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− | a.exp:focus::after {content:"";}
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− | div.exp *{padding:0.3em 10px 0em 10px;}
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− | div.exp *:last-child::after {content:"«";float:right;}
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− | div.exp *:first-child {margin-top:0;}
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− |
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− | ul {margin-bottom: 2em;}
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− | ul li {list-style-position: inside;}
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− | ul li:last-child::after{content:'' !important;}
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− | | + | |
− | | + | |
− | </style>
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− | | + | |
− | | + | |
− | <div class="exp">
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− | <p></p>
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− | </div>
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− | | + | |
− | | + | |
− | <div id=page>
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− |
| + | |
− | <!--UNS pdt#3 DT-->
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− | | + | |
− | <a class="exp" href="#url" tabindex="1">UNS pdt#3 DT</a>
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− | <div class="exp">
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− | <p>
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− | <div class="row">
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− | <div class="col-sm-4" style='padding-left: 8 px; padding-top: 70px;'><IMG SRC="https://static.igem.org/mediawiki/2017/8/81/T--William_and_Mary--pdttag.jpeg" width=320px></div> | + | |
− | <div class="col-sm-8"style='padding-top: 0px; padding-right: 20px; font-size: 18px; font-family: "Optima";'>This part is designed to easily facilitate appending the protein degradation tag (pdt)#3 tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.<br> </br><br> iGEM registry: <a href= "http://parts.igem.org/Part:BBa_K2333401">BBa_K2333401</a> </br></div>
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− | </div>
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− | </p>
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− | </div>
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− | | + | |
− | <!--UNS pdt#3a DT-->
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− | | + | |
− | <a class="exp" href="#url" tabindex="1">UNS pdt#3a DT</a>
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− | <div class="exp">
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− | <p>
| + | |
− | <div class="row">
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− | <div class="col-sm-4" style='padding-left: 8 px; padding-top: 70px;'><IMG SRC="https://static.igem.org/mediawiki/2017/2/23/T--William_and_Mary--pdttaga.jpeg" width=320px></div>
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− | <div class="col-sm-8"style='padding-top: 0px; padding-right: 20px; font-size: 18px; font-family: "Optima";'>This part is designed to easily facilitate appending the protein degradation tag (pdt)#3a tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.<br> </br><br> iGEM registry: <a href= "http://parts.igem.org/Part:BBa_K2333402">BBa_K2333402</a> </br></div>
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− | </div>
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− | </p>
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− | </div>
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− | | + | |
− | <!--UNS pdt#3b DT-->
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− | | + | |
− | <a class="exp" href="#url" tabindex="1">UNS pdt#3b DT</a>
| + | |
− | <div class="exp">
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− | <p>
| + | |
− | <div class="row">
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− | <div class="col-sm-4" style='padding-left: 8 px; padding-top: 70px;'><IMG SRC="https://static.igem.org/mediawiki/2017/9/9b/T--William_and_Mary--pdttagb.jpeg" width=320px></div>
| + | |
− | <div class="col-sm-8"style='padding-top: 0px; padding-right: 20px; font-size: 18px; font-family: "Optima";'>This part is designed to easily facilitate appending the protein degradation tag (pdt)#3b tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.<br> </br><br> iGEM registry: <a href= "http://parts.igem.org/Part:BBa_K2333403">BBa_K2333403</a> </br></div>
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− | </div>
| + | |
− | </p>
| + | |
− | </div>
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− | | + | |
− | <!--UNS pdt#3c DT-->
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− | | + | |
− | <a class="exp" href="#url" tabindex="1">UNS pdt#3c DT</a>
| + | |
− | <div class="exp">
| + | |
− | <p>
| + | |
− | <div class="row">
| + | |
− | <div class="col-sm-4" style='padding-left: 8 px; padding-top: 70px;'><IMG SRC="https://static.igem.org/mediawiki/2017/7/7b/T--William_and_Mary--pdttagc.jpeg" width=320px></div>
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− | <div class="col-sm-8"style='padding-top: 0px; padding-right: 20px; font-size: 18px; font-family: "Optima";'>This part is designed to easily facilitate appending the protein degradation tag (pdt)#3c tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.<br> </br><br> iGEM registry: <a href= "http://parts.igem.org/Part:BBa_K2333404">BBa_K2333404</a> </br></div>
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− | </div>
| + | |
− | </p>
| + | |
− | </div>
| + | |
− | | + | |
− | <!--UNS pdt#3d DT-->
| + | |
− | | + | |
− | <a class="exp" href="#url" tabindex="1">UNS pdt#3d DT</a>
| + | |
− | <div class="exp">
| + | |
− | <p>
| + | |
− | <div class="row">
| + | |
− | <div class="col-sm-4" style='padding-left: 8 px; padding-top: 70px;'><IMG SRC="https://static.igem.org/mediawiki/2017/c/c8/T--William_and_Mary--pdttagd.jpeg" width=320px></div>
| + | |
− | <div class="col-sm-8"style='padding-top: 0px; padding-right: 20px; font-size: 18px; font-family: "Optima";'>This part is designed to easily facilitate appending the protein degradation tag (pdt)#3d tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.<br> </br><br> iGEM registry: <a href= "http://parts.igem.org/Part:BBa_K2333405">BBa_K2333405</a> </br></div>
| + | |
− | </div>
| + | |
− | </p>
| + | |
− | </div>
| + | |
− | | + | |
− | <!--UNS pdt#3e DT-->
| + | |
− | | + | |
− | <a class="exp" href="#url" tabindex="1">UNS pdt#3e DT</a>
| + | |
− | <div class="exp">
| + | |
− | <p>
| + | |
− | <div class="row">
| + | |
− | <div class="col-sm-4" style='padding-left: 8 px; padding-top: 70px;'><IMG SRC="https://static.igem.org/mediawiki/2017/1/1f/T--William_and_Mary--pdttage.jpeg" width=320px></div>
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− | <div class="col-sm-8"style='padding-top: 0px; padding-right: 20px; font-size: 18px; font-family: "Optima";'>This part is designed to easily facilitate appending the protein degradation tag (pdt)#3e tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.<br> </br><br> iGEM registry: <a href= "http://parts.igem.org/Part:BBa_K2333406">BBa_K2333406</a> </br></div>
| + | |
− | </div>
| + | |
− | </p>
| + | |
− | </div>
| + | |
− | | + | |
− | | + | |
− | | + | |
| </div> | | </div> |
| + | </center> |
| | | |
| <div class="container"> | | <div class="container"> |