Difference between revisions of "Team:Heidelberg/SandboxMarita"

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Several secondary metabolites, such as commonly used antibiotics, pigments and detoxifying enzymes, are synthesized by non-ribosomal peptide synthetases (NRPSs). These enzymes beautifully reflect one of the fundamental principles of synthetic biology, as they are remarkably modular. We will assemble new NRPSs by combining individual domains and modules of different origin, thus setting the basis for novel and customized synthesis of non-ribosomal peptidesTo make the use of NRPSs amenable to a wider community, we will devise a new software-tool, called NRPS Designer, which predicts the optimal modular composition of synthetic NRPSs for production of any desired peptide and outputs a cloning.
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Bacon ipsum dolor amet tail cupim andouille ball tip, pancetta ham hock prosciutto beef ribs venison porchetta. Pork chop pork belly flank meatloaf beef kielbasa prosciutto meatball pork loin. Prosciutto tail short loin ham hock rump venison. Flank strip steak kevin chicken ribeye. Picanha tri-tip turducken andouille, pork chop fatback meatball boudin prosciutto tenderloin ribeye. Short ribs leberkas tenderloin, ham ground round beef ribs ball tip boudin short loin.
  
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Meatball pork chop kielbasa ball tip short loin. Beef ribs alcatra sirloin cow turducken spare ribs. Beef meatball ham flank cow sausage ham hock drumstick. Ribeye meatball kielbasa frankfurter, tongue brisket jowl cupim short loin drumstick. Ham hock doner turkey biltong bacon picanha ball tip burgdoggen shank salami prosciutto cow bresaola tri-tip. Meatloaf bacon doner, leberkas ground round picanha sausage pork. Bacon ipsum dolor amet tail cupim andouille ball tip, pancetta ham hock prosciutto beef ribs venison porchetta. Pork chop pork belly flank meatloaf beef kielbasa prosciutto meatball pork loin. Prosciutto tail short loin ham hock rump venison. Flank strip steak kevin chicken ribeye. Picanha tri-tip turducken andouille, pork chop fatback meatball boudin prosciutto tenderloin ribeye. Short ribs leberkas tenderloin, ham ground round beef ribs ball tip boudin short loin. Bacon ipsum dolor amet tail cupim andouille ball tip, pancetta ham hock prosciutto beef ribs venison porchetta. Pork chop pork belly flank meatloaf beef kielbasa prosciutto meatball pork loin. Prosciutto tail short loin ham hock rump venison.
  
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Flank strip steak kevin chicken ribeye. Picanha tri-tip turducken andouille, pork chop fatback meatball boudin prosciutto tenderloin ribeye. Short ribs leberkas tenderloin, ham ground round beef ribs ball tip boudin short loin. Bacon ipsum dolor amet tail cupim andouille ball tip, pancetta ham hock prosciutto beef ribs venison porchetta. Pork chop pork belly flank meatloaf beef kielbasa prosciutto meatball pork loin. Prosciutto tail short loin ham hock rump venison. Flank strip steak kevin chicken ribeye. Picanha tri-tip turducken andouille, pork chop fatback meatball boudin prosciutto tenderloin ribeye. Short ribs leberkas tenderloin, ham ground round beef ribs ball tip boudin short loin. Bacon ipsum dolor amet tail cupim andouille ball tip, pancetta ham hock prosciutto beef ribs venison porchetta. Pork chop pork belly flank meatloaf beef kielbasa prosciutto meatball pork loin. Prosciutto tail short loin ham hock rump venison. Flank strip steak kevin chicken ribeye. Picanha tri-tip turducken andouille, pork chop fatback meatball boudin prosciutto tenderloin ribeye. Short ribs leberkas tenderloin, ham ground round beef ribs ball tip boudin short loin. Flank strip steak kevin chicken ribeye. Picanha tri-tip turducken andouille, pork chop fatback meatball boudin prosciutto tenderloin ribeye. Short ribs leberkas tenderloin, ham ground round beef ribs ball tip boudin short loin. Bacon ipsum dolor amet tail cupim andouille ball tip, pancetta ham hock prosciutto beef ribs venison porchetta. Pork chop pork belly flank meatloaf beef kielbasa prosciutto meatball pork loin. Prosciutto tail short loin ham hock rump venison. Flank strip steak kevin chicken ribeye. Picanha tri-tip turducken andouille, pork chop fatback meatball boudin prosciutto tenderloin ribeye. Short ribs leberkas tenderloin, ham ground round beef ribs ball tip boudin short loin. Bacon ipsum dolor amet tail cupim andouille ball tip, pancetta ham hock prosciutto beef ribs venison porchetta. Pork chop pork belly flank meatloaf beef kielbasa prosciutto meatball pork loin. Prosciutto tail short loin ham hock rump venison. Flank strip steak kevin chicken ribeye. Picanha tri-tip turducken andouille, pork chop fatback meatball boudin prosciutto tenderloin ribeye. Short ribs leberkas tenderloin, ham ground round beef ribs ball tip boudin short loin.
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Meatball pork chop kielbasa ball tip short loin. Beef ribs alcatra sirloin cow turducken spare ribs. Beef meatball ham flank cow sausage ham hock drumstick. Ribeye meatball kielbasa frankfurter, tongue brisket jowl cupim short loin drumstick. Ham hock doner turkey biltong bacon picanha ball tip burgdoggen shank salami prosciutto cow bresaola tri-tip. Meatloaf bacon doner, leberkas ground round picanha sausage pork.
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Meatball pork chop kielbasa ball tip short loin. Beef ribs alcatra sirloin cow turducken spare ribs. Beef meatball ham flank cow sausage ham hock drumstick. Ribeye meatball kielbasa frankfurter, tongue brisket jowl cupim short loin drumstick. Ham hock doner turkey biltong bacon picanha ball tip burgdoggen shank salami prosciutto cow bresaola tri-tip. Meatloaf bacon doner, leberkas ground round picanha sausage pork.
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Flank strip steak kevin chicken ribeye. Picanha tri-tip turducken andouille, pork chop fatback meatball boudin prosciutto tenderloin ribeye. Short ribs leberkas tenderloin, ham ground round beef ribs ball tip boudin short loin. Bacon ipsum dolor amet tail cupim andouille ball tip, pancetta ham hock prosciutto beef ribs venison porchetta. Pork chop pork belly flank meatloaf beef kielbasa prosciutto meatball pork loin. Prosciutto tail short loin ham hock rump venison. Flank strip steak kevin chicken ribeye. Picanha tri-tip turducken andouille, pork chop fatback meatball boudin prosciutto tenderloin ribeye. Short ribs leberkas tenderloin, ham ground round beef ribs ball tip boudin short loin. Bacon ipsum dolor amet tail cupim andouille ball tip, pancetta ham hock prosciutto beef ribs venison porchetta. Pork chop pork belly flank meatloaf beef kielbasa prosciutto meatball pork loin. Prosciutto tail short loin ham hock rump venison.
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Flank strip steak kevin chicken ribeye. Picanha tri-tip turducken andouille, pork chop fatback meatball boudin prosciutto tenderloin ribeye. Short ribs leberkas tenderloin, ham ground round beef ribs ball tip boudin short loin. Flank strip steak kevin chicken ribeye. Picanha tri-tip turducken andouille, pork chop fatback meatball boudin prosciutto tenderloin ribeye. Short ribs leberkas tenderloin, ham ground round beef ribs ball tip boudin short loin. Bacon ipsum dolor amet tail cupim andouille ball tip, pancetta ham hock prosciutto beef ribs venison porchetta. Pork chop pork belly flank meatloaf beef kielbasa prosciutto meatball pork loin. Prosciutto tail short loin ham hock rump venison. Flank strip steak kevin chicken ribeye. Picanha tri-tip turducken andouille, pork chop fatback meatball boudin prosciutto tenderloin ribeye. Short ribs leberkas tenderloin, ham ground round beef ribs ball tip boudin short loin. Bacon ipsum dolor amet tail cupim andouille ball tip, pancetta ham hock prosciutto beef ribs venison porchetta. Pork chop pork belly flank meatloaf beef kielbasa prosciutto meatball pork loin. Prosciutto tail short loin ham hock rump venison. Flank strip steak kevin chicken ribeye. Picanha tri-tip turducken andouille, pork chop fatback meatball boudin prosciutto tenderloin ribeye. Short ribs leberkas tenderloin, ham ground round beef ribs ball tip boudin short loin.
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h1 { margin-top: 10px; font-family: 'Josefin Sans', sans-serif !important; font-size: 58px !important; font-weight: 300 !important; color: #393939 !important; line-height: 58px !important; opacity: 0.9; text-align:center; color: #393939;}
+
−
h2 { margin-top: 10px; font-family: 'Josefin Sans', sans-serif; font-size: 48px; font-weight: 300; color: #393939; line-height: 56px; opacity: 0.9; text-align:center}
+
−
 
+
−
h3 { font-family: 'Josefin Sans', sans-serif; font-size: 40px; font-weight: 300; color: #555; line-height: 40px; }
+
−
h4 { font-family: 'Josefin Sans', sans-serif; font-size: 35px; font-weight: 300; color: #555; line-height: 40px; }
+
−
h5 { font-family: 'Josefin Sans', sans-serif; font-size: 30px; font-weight: 300; color: #555; line-height: 40px; }
+
−
h6 { font-family: 'Josefin Sans', sans-serif; font-size: 25px; font-weight: 300; color: #555; line-height: 40px; }
+
−
h7 { font-family: 'Josefin Sans', sans-serif; font-size: 22px; font-weight: 300; color: #555; line-height: 40px; }
+
−
 
+
−
img { max-width: 100%; }
+
−
 
+
−
.medium-paragraph { font-size: 18px; line-height: 34px; }
+
−
 
+
−
.colored { color: #df6482; }
+
−
 
+
−
::-moz-selection { background: #df6482; color: #fff; text-shadow: none; }
+
−
::selection { background: #df6482; color: #fff; text-shadow: none; }
+
−
 
+
−
/***** General style, all sections *****/
+
−
 
+
−
.section-container { margin: 0 auto; padding-bottom: 80px; }
+
−
.section-description { margin-top: 60px; padding-bottom: 10px; }
+
−
.section-description p { margin-top: 20px; padding: 0 120px; }
+
−
 
+
−
/***** Divider *****/
+
−
 
+
−
.divider-1 { padding: 25px 0 15px 0; }
+
−
.divider-1 .line { width: 160px; margin: 0 auto; border-bottom: 1px dashed #ccc; }
+
−
 
+
−
/***** Top menu *****/
+
−
.navbar {
+
−
margin-bottom: 0;
+
−
    z-index: 10;
+
−
    position: fixed;
+
−
background: white;
+
−
border-bottom: 1px solid white;
+
−
    box-shadow: 2px 0px 30px black ;
+
−
-moz-border-radius: 0; -webkit-border-radius: 0; border-radius: 0;
+
−
}
+
−
 
+
−
.navbar-no-bg { padding-top: 10px; background: white; }
+
−
 
+
−
ul.navbar-nav {
+
−
        padding-top: 10px;
+
−
font-family: 'Josefin Sans', sans-serif;
+
−
font-size: 14px;
+
−
color: #fff;
+
−
font-weight: 400;
+
−
    display: inline-block;
+
−
text-transform: uppercase;
+
−
}
+
−
.navbar-inverse ul.navbar-nav li a { color: grey; border: 0; }
+
−
.navbar-inverse ul.navbar-nav li a:hover { color: #000; border: 0; }
+
−
.navbar-inverse ul.navbar-nav li a:focus { color: #000; outline: 0; border: 0; }
+
−
 
+
−
 
+
−
.navbar-brand {
+
−
width: 100%;
+
−
    position: absolute;
+
−
    top: 1px;
+
−
}
+
−
 
+
−
 
+
−
 
+
−
.navbar>.container .navbar-brand, .navbar>.container-fluid .navbar-brand { margin-left: 0; }
+
−
 
+
−
.dropdown:hover .dropdown-menu {
+
−
    display: block;
+
−
    background-color: white;
+
−
    font-family: 'Josefin Sans', sans-serif;
+
−
font-size: 14px;
+
−
font-weight: 400;
+
−
text-transform: uppercase;
+
−
    border-top: none;
+
−
}
+
−
.dropdown-menu>li>a {
+
−
    color: grey;
+
−
}
+
−
 
+
−
/* Animate */
+
−
 
+
−
 
+
−
 
+
−
.top-content .text h1 { color: #393939; }
+
−
 
+
−
.top-content .description { margin: 30px 0 10px 0; }
+
−
.top-content .description p { opacity: 0.8; }
+
−
.top-content .description a { color: #fff; border-bottom: 1px dotted #fff; }
+
−
.top-content .description a:hover, .top-content .description a:focus { color: #fff; border: 0; }
+
−
 
+
−
 
+
−
/***** Features *****/
+
−
 
+
−
.features-box { margin-top: 30px; text-align: left; }
+
−
.features-box .features-box-icon { font-size: 60px; color: #df6482; line-height: 60px; text-align: center; }
+
−
.features-box h3 { margin-top: 0; padding: 0 0 10px 0; }
+
−
 
+
−
 
+
−
#footer-sec {
+
−
    position: relative;
+
−
    background-color: #393939;
+
−
font-family: 'Josefin Sans', sans-serif;
+
−
}
+
−
#footer-sec h4 {
+
−
color: white;
+
−
 
+
−
}
+
−
#footer-sec  li > a {
+
−
color: grey;
+
−
font-size: 16x;
+
−
}
+
−
#footer-sec  span {
+
−
font-family: 'Josefin Sans', sans-serif;
+
−
color: grey;
+
−
font-size: 16px;
+
−
}
+
−
 
+
−
#footer-sec  li {
+
−
color: grey;
+
−
font-size: 16px;
+
−
list-style: none;
+
−
    padding-bottom: 2px;
+
−
}
+
−
 
+
−
 
+
−
#footer-sec li > a:hover{
+
−
    color: white;
+
−
    text-decoration: none;
+
−
    transition-delay: 0.1s;
+
−
    -webkit-transition: color 1s; /* Safari */
+
−
    transition: color 1s;
+
−
}
+
−
#facebook, #twitter {
+
−
color: grey
+
−
}
+
−
#facebook:hover,#twitter:hover {
+
−
    color: white;
+
−
    transition-delay: 0.1s;
+
−
    -webkit-transition: color 1s; /* Safari */
+
−
    transition: color 1s;
+
−
}
+
−
 
+
−
 
+
−
/***** MEDIA QUERIES *****/
+
−
 
+
−
@media (max-width: 1211px) {
+
−
    .navbar-header {
+
−
        float: none;
+
−
    }
+
−
    .navbar-toggle {
+
−
        display: block;
+
−
    }
+
−
    .navbar-collapse {
+
−
        border-top: 1px solid transparent;
+
−
        box-shadow: inset 0 1px 0 rgba(255,255,255,0.1);
+
−
    }
+
−
    .navbar-collapse.collapse {
+
−
        display: none!important;
+
−
    }
+
−
    .navbar-brand {position: absolute; left: 10px;top: 1px;}
+
−
    #circle:before {
+
−
        height: 61px !important;
+
−
    }
+
−
    .navbar {
+
−
        height: 77px;
+
−
    }
+
−
    .navbar-nav {
+
−
        float: none!important;
+
−
        margin: 7.5px -15px;
+
−
    }
+
−
    .navbar-nav>li {
+
−
        float: none;
+
−
    }
+
−
    .navbar-nav>li>a {
+
−
        padding-top: 10px;
+
−
        padding-bottom: 10px;
+
−
    }
+
−
    .navbar-text {
+
−
        float: none;
+
−
        margin: 15px 0;
+
−
    }
+
−
    /* since 3.1.0 */
+
−
    .navbar-collapse.collapse.in {
+
−
        display: block!important;
+
−
    }
+
−
    .collapsing {
+
−
        overflow: hidden!important;
+
−
    }
+
−
}
+
−
   
+
−
 
+
−
 
+
−
 
+
−
 
+
−
 
+
−
 
+
−
#circle {
+
−
    position: relative;
+
−
    box-shadow: 2px 0px 30px black ;
+
−
    background-color: blue;
+
−
    height: 120px;
+
−
    width: 120px;
+
−
    border-radius: 50%;
+
−
   
+
−
   
+
−
}
+
−
 
+
−
#circle:before{
+
−
    position: absolute;
+
−
    content: "";
+
−
    width: 260%;
+
−
    height: 60px;
+
−
   
+
−
    left: -90%;
+
−
    background: white;
+
−
}
+
−
 
+
−
 
+
−
#circle:after{
+
−
    position: absolute;
+
−
    content: "";
+
−
    width: 100%;
+
−
    height: 100%;
+
−
    left: 0;
+
−
    top: 0;
+
−
    background: white;
+
−
    border-radius: 50%;
+
−
    background-image: url('https://static.igem.org/mediawiki/2017/8/8f/Heidelberg_2017_phage_logo.svg');
+
−
    background-size: 90px 90px;
+
−
    background-repeat: no-repeat;
+
−
    background-position: center;
+
−
}
+
−
 
+
−
 
+
−
 
+
−
  .input_form {
+
−
                        background-color: #939393;
+
−
                        border: none;
+
−
                        color: white;
+
−
                        padding: 5px 32px;
+
−
                        margin-top: 25px;
+
−
                        text-align: center;
+
−
                        text-decoration: none;
+
−
                        display: inline-block;
+
−
                        font-size: 16px; 
+
−
                        }
+
−
                       
+
−
                        input[type="text"] {
+
−
                          display: block;
+
−
                          margin: 0;
+
−
                          width: 100%;
+
−
                          height: 30px;
+
−
                          font-size: 18px;
+
−
                          -webkit-appearance: none;
+
−
                          -moz-appearance: none;
+
−
                          appearance: none;
+
−
                          -webkit-box-shadow: none;
+
−
                          -moz-box-shadow: none;
+
−
                          box-shadow: none;
+
−
                          -webkit-border-radius: none;
+
−
                          -moz-border-radius: none;
+
−
                          -ms-border-radius: none;
+
−
                          -o-border-radius: none;
+
−
                          border-radius: none;
+
−
                        }
+
−
                       
+
−
                        #sidenav {
+
−
                            font-family: 'Josefin Sans', sans-serif;
+
−
                            font-size: 14px;
+
−
                            color: #fff;
+
−
                            font-weight: 400;
+
−
                            text-transform: uppercase;
+
−
                           
+
−
                          list-style: none;
+
−
                        }
+
−
                        #sidenav {
+
−
                            font-family: 'Josefin Sans', sans-serif;
+
−
                            font-size: 14px;
+
−
                            color: #fff;
+
−
                            font-weight: 400;
+
−
                            text-transform: uppercase;
+
−
                           
+
−
                        }
+
−
                        #sidenav > li > ul > li {
+
−
                            list-style: none;
+
−
                        }
+
−
                        #sidenav > li > ul > li > a {
+
−
                            color: firebrick;
+
−
                        }
+
−
                        #sidenav > li > ul > li > a:hover {
+
−
                            color: #393939;
+
−
                            transition: color 1s ease;
+
−
                        }
+
−
                       
+
−
                        .navmenu-brand > img {
+
−
                            border-radius: 200px;
+
−
                            width: 100px;
+
−
                            height: auto;
+
−
                        }
+
−
                       
+
−
                        .navmenu-brand {
+
−
                            text-align: center;
+
−
                            font-size: 20px;
+
−
                            color: #fff;
+
−
                            font-weight: 400;
+
−
                           
+
−
                           
+
−
                        }
+
−
               
+
−
               
+
−
</style>
+
−
<link rel="stylesheet" type="text/css"
+
−
href="https://2017.igem.org/Template:Heidelberg/table_layout/CSS?action=raw&amp;ctype=text/css" />
+
−
                        <!-- Favicon and touch icons -->
+
−
<script src="https://ajax.googleapis.com/ajax/libs/jquery/3.2.1/jquery.min.js"></script>
+
−
 
+
−
 
+
−
 
+
−
 
+
−
<script type="text/javascript" src="https://2015.igem.org/Template:Heidelberg/js/jasnybootstrapmin?action=raw&amp;ctype=text/javascript"></script>
+
−
 
+
−
                        <link href="https://maxcdn.bootstrapcdn.com/font-awesome/4.2.0/css/font-awesome.min.css" rel="stylesheet">
+
−
 
+
−
<!-- jQuery CDN -->
+
−
        <script src="https://code.jquery.com/jquery-1.12.0.min.js"></script>
+
−
        <!-- Bootstrap Js CDN -->
+
−
        <script src="https://maxcdn.bootstrapcdn.com/bootstrap/3.3.7/js/bootstrap.min.js"></script>
+
−
        <!-- jQuery Nicescroll CDN -->
+
−
        <script src="https://cdnjs.cloudflare.com/ajax/libs/jquery.nicescroll/3.6.8-fix/jquery.nicescroll.min.js"></script>
+
−
<style>
+
−
   
+
−
    #sidebarCollapse {
+
−
        background-color: white;
+
−
    }
+
−
    #overall-wrapper {
+
−
        min-width: 700px;
+
−
    }
+
−
    body {
+
−
        background-color: #010101;
+
−
    }
+
−
      #textblock1 {
+
−
          position: absolute;
+
−
          top: 200px;
+
−
          left: 0px;
+
−
          width: 500px;
+
−
      }     
+
−
      #textblock2 {
+
−
          position: absolute;
+
−
          top: 1050px;
+
−
          left: 150px;
+
−
          width: 500px;
+
−
      } 
+
−
      #textblock3 {
+
−
            position: absolute;
+
−
          top: 950px;
+
−
          right: 50px;
+
−
          width: 500px;
+
−
          height: 400px;
+
−
         
+
−
      }
+
−
      #textblock4 {
+
−
            position: absolute;
+
−
          top: 1600px;
+
−
          left: 100px;
+
−
          width: 500px;
+
−
      }
+
−
      #textblock5 {
+
−
            position: absolute;
+
−
          top: 2150px;
+
−
          right: 100px;
+
−
          width: 600px;
+
−
      }
+
−
      #textblock6 {
+
−
            position: absolute;
+
−
          top: 2700px;
+
−
          left: 0px;
+
−
          width: 500px;
+
−
      },
+
−
        #textblock6 {
+
−
            position: absolute;
+
−
          top: 2800px;
+
−
          left: 0px;
+
−
          width: 400px;
+
−
      }
+
−
    #textblock7 {
+
−
            position: absolute;
+
−
          top: 3250px;
+
−
          right: 100px;
+
−
          width: 400px;
+
−
      }
+
−
#textblock1 > p, #textblock3 > p, #textblock5 > p,#textblock2 > p, #textblock4 > p, #textblock6 > p, #textblock7 > p {
+
−
    color: white !important;
+
−
    text-align: center;
+
−
    font-size: 28px !important;
+
−
    font-weight: 300 !important;
+
−
}
+
−
 
+
−
#textblock1 > a {
+
−
    color: #F8991D;
+
−
    font-size: 50px;
+
−
}
+
−
#textblock1 > a:hover, #textblock2 > a:hover, #textblock3 > a:hover, #textblock4 > a:hover, #textblock5 > a:hover, #textblock6 > a:hover, #textblock7 > a:hover, #textblock8 > a:hover  {
+
−
    text-decoration: underline;
+
−
}
+
−
#textblock2 > a {
+
−
    color: whitesmoke;
+
−
    font-size: 50px;
+
−
    line-height: 50px;
+
−
}
+
−
 
+
−
#textblock4 > a {
+
−
  color:#FEE000;
+
−
    font-size: 50px;
+
−
    line-height: 50px;
+
−
}
+
−
#textblock5 > a {
+
−
  color:whitesmoke;
+
−
    font-size: 50px;
+
−
    line-height: 50px;
+
−
}
+
−
#textblock6 > a {
+
−
  color:#9D1C20;
+
−
    font-size: 50px;
+
−
    line-height: 50px;
+
−
}
+
−
 
+
−
 
+
−
#textblock7 > a {
+
−
    color: #6698be;
+
−
    font-size: 50px;
+
−
    line-height: 50px;
+
−
}
+
−
#textblock8 > a {
+
−
    color: #393939;
+
−
    font-size: 50px;
+
−
}
+
−
 
+
−
    #toolbox-icon1 {
+
−
position: absolute;
+
−
top: 4460px;
+
−
left: 430px;
+
−
} 
+
−
   
+
−
    #toolbox-row1 {
+
−
        top: 0px;
+
−
    }
+
−
    #toolbox-row2 {
+
−
        top: 200px;
+
−
    }
+
−
    #toolbox-row3 {
+
−
        top: 400px;
+
−
    }
+
−
    wrapper {
+
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−
                         
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+
−
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      }
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−
     
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−
      #Icon1:hover > .icon-text > p,#Icon2:hover > .icon-text > p,#Icon3:hover > .icon-text > p,#Icon4:hover > .icon-text-right > p,#Icon5:hover > .icon-text-right > p,#Icon6:hover > .icon-text-right > p {
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          transition: color 1s ease;
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          color: white !important;
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      #Icon1 > .icon-text > p,#Icon2 > .icon-text > p,#Icon3 > .icon-text > p,#Icon4 > .icon-text-right > p,#Icon5 > .icon-text-right > p,#Icon6 > .icon-text-right > p  {
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          visibility: hidden!important;
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      #Icon2 > .icon-text > h5 {
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          font-weight: 400 !important;
+
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          line-height: 40px !important;
+
−
      } 
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+
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      #Icon3 > .icon-text > h5 {
+
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          text-transform: uppercase;
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          color: #6698BE !important;
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          font-weight: 400 !important;
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          line-height: 40px !important;
+
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+
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      #Icon4 > .icon-text-right > h5 {
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          text-transform: uppercase;
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          color: #FEE000 !important;
+
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          font-weight: 400 !important;
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          line-height: 40px !important;
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      }
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+
−
     
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      #Icon5 > .icon-text-right > h5 {
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          text-transform: uppercase;
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          font-weight: 400 !important;
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          line-height: 40px !important;
+
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      }
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−
 
+
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      #Icon6 > .icon-text-right > h5 {
+
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          text-transform: uppercase;
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          color: #9D1C20 !important;
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          font-weight: 400 !important;
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          line-height: 40px !important;
+
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      }
+
−
     
+
−
      .phage-scroller {
+
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              width: 150px;
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              height: auto;
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+
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      #toolbox {
+
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          position: absolute;
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          top: 3800px;
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          min-height: 600px ;
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          width: 100%;
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          max-width: 1200px;
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          margin: 0 auto;
+
 
            
 
            
−
      }
+
        </tr>
−
     
+
    </thead>
−
      #toolbox-image {
+
     <tbody>
−
          position: absolute;
+
        <tr>
−
          left: 380px;
+
             <td>Initial denaturation</td>
−
          width: 500px;
+
            <td>98</td>
−
          top: 10%
+
            <td>30 seconds</td>
−
      }
+
        </tr>
−
      
+
        <tr>
−
      .icon {
+
            <td>25-35 cycles</td>
−
             cursor: pointer;
+
            <td>98 (denaturation)<br>
−
      }
+
                45-72 (annealing) <a href=""#Note1">see" Note 1</a><br>
−
      .toolbox-icon {
+
                72 (extension)</td>
−
          width: 120px;
+
             <td>5-10 seconds <br>
−
          height: 120px;
+
                10-30 seconds<br>
−
          border-radius: 100%;
+
                15-30 seconds per kb</td>
−
          float: left;
+
        </tr>
−
          background-color: white;
+
        <tr>
−
          position: absolute;
+
             <td>Final extension</td>
−
         
+
            <td>72</td>
−
      }
+
             <td>2-5 minutes</td>
−
     
+
         </tr>
−
      .icon:hover > .toolbox-icon {
+
        <tr>
−
         
+
             <td>Hold</td>
−
          transform: scale(1.2,1.2);
+
             <td>4</td>
−
          transition: all 1s ease-out;
+
             <td>Indefinitely</td>
−
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+
         </tr>
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          #toolbox {
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              top: 3600px;
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−
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          #toolbox-image {
+
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              position: relative;
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              left: 30px !important;
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          #Icon1 {
+
−
              left: 100px;
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+
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+
−
              left: 100px;
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              left: 100px;
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+
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              right: 200px;
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+
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              right: 200px;
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+
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              right: 200px;
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      @media (max-width: 960px) {
+
−
       
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−
            #wrapper {
+
−
              padding-bottom: 980px;
+
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−
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             .phage-scroller {
+
−
              width: 100px;
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              height: auto;
+
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+
−
              #textblock1 {
+
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          position: absolute;
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        #textblock1 > p,#textblock2 > p, #textblock3 > p,#textblock4 > p, #textblock5 > p,#textblock6 > p, #textblock7 > p {
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+
−
            font-size: 20px !important;
+
−
            font-weight: 300 !important;
+
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+
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          #textblock1 > a,#textblock2 > a, #textblock4 > a,  #textblock6 > a, #textblock7 > a {
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+
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+
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+
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+
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+
−
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+
−
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+
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+
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+
−
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+
−
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+
−
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+
−
          width: 300px;
+
−
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+
−
         
+
−
          #toolbox {
+
−
              top: 2300px;
+
−
          }
+
−
         
+
−
         
+
−
          #toolbox-image {
+
−
              position: relative;
+
−
              left: 30px !important;
+
−
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+
−
          #Icon1 {
+
−
              left: 100px;
+
−
              top: 0px;
+
−
          }
+
−
          #Icon2 {
+
−
              left: 100px;
+
−
              top: 150px;
+
−
          }
+
−
          #Icon3 {
+
−
              left: 100px;
+
−
              top: 300px;
+
−
          }
+
−
          #Icon4 {
+
−
              right: 200px;
+
−
              top: 150px;
+
−
          }
+
−
          #Icon5 {
+
−
              right: 200px;
+
−
              top: 300px; 
+
−
          }
+
−
          #Icon6 {
+
−
              right: 200px;
+
−
              top: 450px;
+
−
          }
+
−
           
+
−
      }
+
−
     
+
−
   
+
−
     
+
−
     
+
  
−
     
+
    </tbody>
−
     
+
</table>
−
     
+
}} |
−
     
+
Here you can see a whole lot of shite in a table.
−
+
Here you can see a whole lot of shite in a table.
−
.fa-twitter{
+
Here you can see a whole lot of shite in a table.
−
background: #393939;
+
Here you can see a whole lot of shite in a table.
−
color: #FFFFFF;
+
Here you can see a whole lot of shite in a table.
−
  padding: 0.1em 0.1em;
+
Here you can see a whole lot of shite in a table.
−
    font-size: 2em;
+
Here you can see a whole lot of shite in a table.
−
}
+
Here you can see a whole lot of shite in a table.
−
    .fa-facebook{
+
Here you can see a whole lot of shite in a table.
−
background: #393939;
+
Here you can see a whole lot of shite in a table.
−
color: #FFFFFF;
+
Here you can see a whole lot of shite in a table.
−
  padding: 0.1em 0.3em;
+
Here you can see a whole lot of shite in a table.
−
        font-size: 2em;
+
Here you can see a whole lot of shite in a table.
−
}
+
Here you can see a whole lot of shite in a table.
 +
Here you can see a whole lot of shite in a table.
 +
Here you can see a whole lot of shite in a table.
 +
Here you can see a whole lot of shite in a table.
 +
}}
 +
}}
  
−
                    </style>
+
{{#tag:html|
−
         
+
<div class="row content">
−
</head>
+
<div class="col-lg-12 col-md-12 col-xs-12" style="padding-top: 20px; padding-bottom: 50px; display: block">
−
<body class="t-body">
+
<div class="mdl-shadow--4dp" style="padding: 20px">
−
      <div id="overall-wrapper">
+
<h3> References </h3>
−
    <!-- Top menu -->
+
<p id="references"></p>
−
<nav class="navbar navbar-inverse navbar-fixed-top navbar-no-bg " role="navigation">
+
<p>[1]<a>Here comes a link</a></p>
−
<div class="container">
+
−
<div class="navbar-header">
+
−
                 
+
−
                      <a class="navbar-brand" href="https://2017.igem.org/Team:Heidelberg"><div id="circle">
+
−
                        </div></a>
+
−
              <a type="button" data-canvas="body" id="sidebarCollapse" class="navbar-toggle" data-toggle="offcanvas" data-target="#sidebar" style="color: #393939;margin-top: 10px;">SIDEBAR 
+
−
                    </a>
+
−
             
+
−
</div>
+
−
<!-- Collect the nav links, forms, and other content for toggling -->
+
−
<div class="collapse navbar-collapse" id="bs-example-navbar-collapse-1">
+
−
<ul class="nav navbar-nav navbar-right">
+
−
<li><a href="https://2017.igem.org/Team:Heidelberg/Achievements">Achievements</a></li>
+
−
<li class="dropdown"><a href="https://2017.igem.org/Team:Heidelberg/Description">Project <span class="caret"></span></a>
+
−
                        <ul class="dropdown-menu">
+
−
                        <li><a href="https://2017.igem.org/Team:Heidelberg/Description">Overview</a></li> 
+
−
                        <li><a href="https://2017.igem.org/Team:Heidelberg/Results">Results</a></li>
+
−
                        <li><a href="https://2017.igem.org/Team:Heidelberg/Parts">Parts</a></li>
+
−
                        </ul>
+
−
                        </li>
+
−
<li class="dropdown"><a href="https://2017.igem.org/Team:Heidelberg/Model">Modeling <span class="caret"></span></a>
+
−
                        <ul class="dropdown-menu">
+
−
                        <li><a href="https://2017.igem.org/Team:Heidelberg/iGEMGoesGreen">iGEM goes Green</a></li> 
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                                                                    <a href="#" id="accelerated_evolution"><strong>Accelerated evolution</strong></a>
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                                                                    <p>by using Phage-assisted continious evolution (PACE) and our newly created method called PREDCEL</p>
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                                                                    <p>Let us introduce you to the next generation of directed evolution...</p>
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                                                                    <a href="#"><strong>Come discover the diversity of Nature</strong></a>
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                                                                    <p>As finding the right startersequence is quite difficult we used</p>
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                                                                    <a href="#" id="artificial_intelligence"><strong>Artificial Intelligence</strong></a>
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                                                                    <p>for protein classification & generation of entirely new functional sequences</p>
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                                                                    <a href="#"><strong>Carbon-Silicon Bond Formation</strong></a>
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                                                                    <p> which could ultimately affect a lot of different aspects of our life and life in general</p>
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                                                                <p>In order to make PREDCEL work, we also had a lot to model</p><br><p> Therefore check out our</p>
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                                                                    <a href="#"><strong>Interactive modeling</strong></a>
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                                                        <p> As we affect the world & <br> the world affects us, we need</p>
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                                                                <div id="Icon5" class="icon"><img src="https://static.igem.org/mediawiki/2017/4/4b/T--Heidelberg--2017_T--Heidelberg--2017_Icon_Protein_Interaction.svg" class="toolbox-icon" style="left: 270px;"><div class="icon-text-right"><h5>Protein Interaction</h5><p>Interact</p></div></div>
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                                                                  <div id="Icon6" class="icon"><img src="https://static.igem.org/mediawiki/2017/9/9c/T--Heidelberg--2017_T--Heidelberg--2017_Icon_Product_Specificity.svg" class="toolbox-icon" style="left: 250px;"><div class="icon-text-right"><h5>Protein Specificity</h5><p>Specify</p></div></div>
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Revision as of 14:12, 27 October 2017

Our Project.

Foundational Advance in Peptide Synthesis.

TITLE

Several secondary metabolites, such as commonly used antibiotics, pigments and detoxifying enzymes, are synthesized by non-ribosomal peptide synthetases (NRPSs). These enzymes beautifully reflect one of the fundamental principles of synthetic biology, as they are remarkably modular. We will assemble new NRPSs by combining individual domains and modules of different origin, thus setting the basis for novel and customized synthesis of non-ribosomal peptidesTo make the use of NRPSs amenable to a wider community, we will devise a new software-tool, called NRPS Designer, which predicts the optimal modular composition of synthetic NRPSs for production of any desired peptide and outputs a cloning.

Heading 1

Eye of the Tiger
Bacon ipsum dolor amet tail cupim andouille ball tip, pancetta ham hock prosciutto beef ribs venison porchetta. Pork chop pork belly flank meatloaf beef kielbasa prosciutto meatball pork loin. Prosciutto tail short loin ham hock rump venison. Flank strip steak kevin chicken ribeye. Picanha tri-tip turducken andouille, pork chop fatback meatball boudin prosciutto tenderloin ribeye. Short ribs leberkas tenderloin, ham ground round beef ribs ball tip boudin short loin.
Bacon ipsum dolor amet tail cupim andouille ball tip, pancetta ham hock prosciutto beef ribs venison porchetta. Pork chop pork belly flank meatloaf beef kielbasa prosciutto meatball pork loin. Prosciutto tail short loin ham hock rump venison. Flank strip steak kevin chicken ribeye. Picanha tri-tip turducken andouille, pork chop fatback meatball boudin prosciutto tenderloin ribeye. Short ribs leberkas tenderloin, ham ground round beef ribs ball tip boudin short loin.
Fig: 1 Telling you something about stuff
No I´m going to describe the graphic. Hey there what´s ging on No I´m going to describe the graphic. Hey there what´s ging on
Meatball pork chop kielbasa ball tip short loin. Beef ribs alcatra sirloin cow turducken spare ribs. Beef meatball ham flank cow sausage ham hock drumstick. Ribeye meatball kielbasa frankfurter, tongue brisket jowl cupim short loin drumstick. Ham hock doner turkey biltong bacon picanha ball tip burgdoggen shank salami prosciutto cow bresaola tri-tip. Meatloaf bacon doner, leberkas ground round picanha sausage pork. Bacon ipsum dolor amet tail cupim andouille ball tip, pancetta ham hock prosciutto beef ribs venison porchetta. Pork chop pork belly flank meatloaf beef kielbasa prosciutto meatball pork loin. Prosciutto tail short loin ham hock rump venison. Flank strip steak kevin chicken ribeye. Picanha tri-tip turducken andouille, pork chop fatback meatball boudin prosciutto tenderloin ribeye. Short ribs leberkas tenderloin, ham ground round beef ribs ball tip boudin short loin. Bacon ipsum dolor amet tail cupim andouille ball tip, pancetta ham hock prosciutto beef ribs venison porchetta. Pork chop pork belly flank meatloaf beef kielbasa prosciutto meatball pork loin. Prosciutto tail short loin ham hock rump venison.
Fig: 1 Telling you something about stuff
No I´m going to describe the graphic. Hey there what´s ging on No I´m going to describe the graphic. Hey there what´s ging on
Flank strip steak kevin chicken ribeye. Picanha tri-tip turducken andouille, pork chop fatback meatball boudin prosciutto tenderloin ribeye. Short ribs leberkas tenderloin, ham ground round beef ribs ball tip boudin short loin. Bacon ipsum dolor amet tail cupim andouille ball tip, pancetta ham hock prosciutto beef ribs venison porchetta. Pork chop pork belly flank meatloaf beef kielbasa prosciutto meatball pork loin. Prosciutto tail short loin ham hock rump venison. Flank strip steak kevin chicken ribeye. Picanha tri-tip turducken andouille, pork chop fatback meatball boudin prosciutto tenderloin ribeye. Short ribs leberkas tenderloin, ham ground round beef ribs ball tip boudin short loin. Bacon ipsum dolor amet tail cupim andouille ball tip, pancetta ham hock prosciutto beef ribs venison porchetta. Pork chop pork belly flank meatloaf beef kielbasa prosciutto meatball pork loin. Prosciutto tail short loin ham hock rump venison. Flank strip steak kevin chicken ribeye. Picanha tri-tip turducken andouille, pork chop fatback meatball boudin prosciutto tenderloin ribeye. Short ribs leberkas tenderloin, ham ground round beef ribs ball tip boudin short loin. Flank strip steak kevin chicken ribeye. Picanha tri-tip turducken andouille, pork chop fatback meatball boudin prosciutto tenderloin ribeye. Short ribs leberkas tenderloin, ham ground round beef ribs ball tip boudin short loin. Bacon ipsum dolor amet tail cupim andouille ball tip, pancetta ham hock prosciutto beef ribs venison porchetta. Pork chop pork belly flank meatloaf beef kielbasa prosciutto meatball pork loin. Prosciutto tail short loin ham hock rump venison. Flank strip steak kevin chicken ribeye. Picanha tri-tip turducken andouille, pork chop fatback meatball boudin prosciutto tenderloin ribeye. Short ribs leberkas tenderloin, ham ground round beef ribs ball tip boudin short loin. Bacon ipsum dolor amet tail cupim andouille ball tip, pancetta ham hock prosciutto beef ribs venison porchetta. Pork chop pork belly flank meatloaf beef kielbasa prosciutto meatball pork loin. Prosciutto tail short loin ham hock rump venison. Flank strip steak kevin chicken ribeye. Picanha tri-tip turducken andouille, pork chop fatback meatball boudin prosciutto tenderloin ribeye. Short ribs leberkas tenderloin, ham ground round beef ribs ball tip boudin short loin.

Buttageddon

Meatball pork chop kielbasa ball tip short loin. Beef ribs alcatra sirloin cow turducken spare ribs. Beef meatball ham flank cow sausage ham hock drumstick. Ribeye meatball kielbasa frankfurter, tongue brisket jowl cupim short loin drumstick. Ham hock doner turkey biltong bacon picanha ball tip burgdoggen shank salami prosciutto cow bresaola tri-tip. Meatloaf bacon doner, leberkas ground round picanha sausage pork.

Formageddon

Meatball pork chop kielbasa ball tip short loin. Beef ribs alcatra sirloin cow turducken spare ribs. Beef meatball ham flank cow sausage ham hock drumstick. Ribeye meatball kielbasa frankfurter, tongue brisket jowl cupim short loin drumstick. Ham hock doner turkey biltong bacon picanha ball tip burgdoggen shank salami prosciutto cow bresaola tri-tip. Meatloaf bacon doner, leberkas ground round picanha sausage pork.

Listmageddon

  1. FOO
  2. BAR
  3. BAZ
    1. FOOBAR
    2. FOOBAZ
    3. QUUX

BACON Ipsum!

Flank strip steak kevin chicken ribeye. Picanha tri-tip turducken andouille, pork chop fatback meatball boudin prosciutto tenderloin ribeye. Short ribs leberkas tenderloin, ham ground round beef ribs ball tip boudin short loin. Bacon ipsum dolor amet tail cupim andouille ball tip, pancetta ham hock prosciutto beef ribs venison porchetta. Pork chop pork belly flank meatloaf beef kielbasa prosciutto meatball pork loin. Prosciutto tail short loin ham hock rump venison. Flank strip steak kevin chicken ribeye. Picanha tri-tip turducken andouille, pork chop fatback meatball boudin prosciutto tenderloin ribeye. Short ribs leberkas tenderloin, ham ground round beef ribs ball tip boudin short loin. Bacon ipsum dolor amet tail cupim andouille ball tip, pancetta ham hock prosciutto beef ribs venison porchetta. Pork chop pork belly flank meatloaf beef kielbasa prosciutto meatball pork loin. Prosciutto tail short loin ham hock rump venison.

BACON Ipsum!

Flank strip steak kevin chicken ribeye. Picanha tri-tip turducken andouille, pork chop fatback meatball boudin prosciutto tenderloin ribeye. Short ribs leberkas tenderloin, ham ground round beef ribs ball tip boudin short loin. Flank strip steak kevin chicken ribeye. Picanha tri-tip turducken andouille, pork chop fatback meatball boudin prosciutto tenderloin ribeye. Short ribs leberkas tenderloin, ham ground round beef ribs ball tip boudin short loin. Bacon ipsum dolor amet tail cupim andouille ball tip, pancetta ham hock prosciutto beef ribs venison porchetta. Pork chop pork belly flank meatloaf beef kielbasa prosciutto meatball pork loin. Prosciutto tail short loin ham hock rump venison. Flank strip steak kevin chicken ribeye. Picanha tri-tip turducken andouille, pork chop fatback meatball boudin prosciutto tenderloin ribeye. Short ribs leberkas tenderloin, ham ground round beef ribs ball tip boudin short loin. Bacon ipsum dolor amet tail cupim andouille ball tip, pancetta ham hock prosciutto beef ribs venison porchetta. Pork chop pork belly flank meatloaf beef kielbasa prosciutto meatball pork loin. Prosciutto tail short loin ham hock rump venison. Flank strip steak kevin chicken ribeye. Picanha tri-tip turducken andouille, pork chop fatback meatball boudin prosciutto tenderloin ribeye. Short ribs leberkas tenderloin, ham ground round beef ribs ball tip boudin short loin.

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Step Temperature (°C) Time
Initial denaturation 98 30 seconds
25-35 cycles 98 (denaturation)
45-72 (annealing) see" Note 1
72 (extension)		 5-10 seconds
10-30 seconds
15-30 seconds per kb
Final extension 72 2-5 minutes
Hold 4 Indefinitely

References

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