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                 <h1>Synthesis of the Repeats</h1>
 
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Revision as of 21:15, 28 October 2017

RNA Organelle

RNA Aggregation

In synthetic biology, we are often introducing new pathways to bacteria that do not naturally express them. The novel pathway will produce exotic enzymes and proteins which the host bacteria will not necessarily have the internal environment to organise these macromolecular products, this could be detrimental to the performance of both the pathway and the organism itself. Additionally, depending on the organism used, the activity of the pathway can vary and be difficult to characterise against other models used. Thus, we aim to standardize the microenvironmental activity of different pathways within the cell by localising the associated enzymes/proteins in an RNA based structure, leading to the pathway to act in a predictable way, regardless of the organism.
Fig. 1: A) Representation of single-stranded RNA that forms the liquid-liquid phase separated-like structure. B) Images of RNA aggregates formed inside the nucleus of different mutant mammalian cells that express RNA containing either the repeats CUG or CAG
In mammalian cells, RNA containing triplet repeats of nucleotides such as CAGCAGCAGCAG have been observed to aggregate in the nucleus. The properties of the RNA aggregation has been observed to be similar to those seen in liquid-liquid phase separated molecules, which can be visualized as oil droplets in water. The densely compact RNA strands will allow small molecules or substrates to pass through the structure while maintaining a different internal environment. Using this idea, we aimed to express RNA containing repeats in bacterial cells, in order to develop an intracellular scaffold.

Synthesis of the Repeats


Centre for Research and Interdisciplinarity (CRI)
Faculty of Medicine Cochin Port-Royal, South wing, 2nd floor
Paris Descartes University
24, rue du Faubourg Saint Jacques
75014 Paris, France
bettencourt.igem2017@gmail.com