Difference between revisions of "Team:Heidelberg/SandboxCathy"

(Created page with "{{Heidelberg/header}} {{Heidelberg/navbar}} {{Heidelberg/main| Title| Subtitle| URL to image| color|...")
 
Line 3: Line 3:
  
 
{{Heidelberg/main|               
 
{{Heidelberg/main|               
−
     Title|
+
     Optogenetics|
−
     Subtitle|
+
     Modulator of selection stringency|
 
     URL to image|
 
     URL to image|
 
     color|                           
 
     color|                           
 
     {{Heidelberg/abstract|       
 
     {{Heidelberg/abstract|       
 
         Image URL|
 
         Image URL|
−
        Content
+
The generation of proteins with radically altered or highly specific new activities is a very challenging task in the field of directed evolution. Modulation of selection stringency is indispensable for the optimization of proteins, which in their native form exhibit a lack of activity for the desired action. To extent the possibilities of PACE/PREDCEL based protein optimization and directly evolve new activities from an inactive starting gene without the use of intermediate evolutionary stepping stones, we provide an optogenetic modulator of selection stringency comprises the blue light-dependent transcription factor EL222 and a bidirectional promoter system that can induce or repress gIII expression under blue light in a non-toxic, rapidly delivered and reversible manner. In this subproject we were able to demonstrate a light-dependent increase in phage propagation using the pBLind-geneIII cassette under blue light. This part of our toolbox enables the scientific community to easily adapt the selection stringency in PACE and PREDCEL experiments and leads to a bright future for protein optimization. }}
−
    }}
+
 
     {{Heidelberg/templateus/Contentsection|
 
     {{Heidelberg/templateus/Contentsection|
 
         {{#tag:html|
 
         {{#tag:html|

Revision as of 12:28, 31 October 2017


Optogenetics
Modulator of selection stringency
The generation of proteins with radically altered or highly specific new activities is a very challenging task in the field of directed evolution. Modulation of selection stringency is indispensable for the optimization of proteins, which in their native form exhibit a lack of activity for the desired action. To extent the possibilities of PACE/PREDCEL based protein optimization and directly evolve new activities from an inactive starting gene without the use of intermediate evolutionary stepping stones, we provide an optogenetic modulator of selection stringency comprises the blue light-dependent transcription factor EL222 and a bidirectional promoter system that can induce or repress gIII expression under blue light in a non-toxic, rapidly delivered and reversible manner. In this subproject we were able to demonstrate a light-dependent increase in phage propagation using the pBLind-geneIII cassette under blue light. This part of our toolbox enables the scientific community to easily adapt the selection stringency in PACE and PREDCEL experiments and leads to a bright future for protein optimization.
Basic content, here texts are written. General rules: if html tags (the things starting with "<" and ending with ">") occur, the whole block in which they occur, have to be inside the following (replacing 'content') content Pages on the wiki can be edited or created by going the URL where the page shall be and choosing wiki tools -> Edit/Create Simply paste the text there and save the page. Images can be uploaded by choosing 'upload files' under 'wiki tools'. Filenames shall be T--Heidelberg--Team_Heidelberg_2017_real-name.png If a file was uploaded, a page showing the file occurs, click on 'original file' and take the URL you are getting to. This URL is the image URL used to embed the image. An editor that matches braces is pretty useful. Check if the braces of main are close properly and the chances are good thta the page will work. headers can be written as follows:

Large header

or in smaller:

Smaller Header

this is bold text this produces a line break
This text is italic Referencing works iGEMHD. Links to other pages work like this. Images can be embedded over the half page:
Title
Subtitle
Or over the full page:
Title
Subtitle
HTML tables can be generated from excel tables via http://tableizer.journalistopia.com/tableizer.php. Please delete the 'class="tableizer-table"' and 'class="tableizer-firstrow"' They are put in tableboxes as follows:

Title Subtitle

html table

References