Difference between revisions of "Team:Heidelberg/Software/MAWS"

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         Image URL| As aptamer-based riboswitches are the most promising regulatory element to couple the PACE/PREDCEL selection system to the intracellular level of a small molecule, the availbability of suitable aptamers is the limiting factor for enzyme PACE/PREDCEL experiments. The state of the art method to generate aptamers and riboswitches is based on the <i>in vitro</i> selection of sequences from a random oligonucleotide library using the SELEX method. This requires the establishment of suitable protocols and can be labor and cost intensive. Therefore the 2015 iGEM Heidelberg team developed a aptamer design tool called MAWS (Making Aptamers Without SELEX) which tries to predict aptamers by progressive selection of appropriate bases using an entropic criterion. }}
 
         Image URL| As aptamer-based riboswitches are the most promising regulatory element to couple the PACE/PREDCEL selection system to the intracellular level of a small molecule, the availbability of suitable aptamers is the limiting factor for enzyme PACE/PREDCEL experiments. The state of the art method to generate aptamers and riboswitches is based on the <i>in vitro</i> selection of sequences from a random oligonucleotide library using the SELEX method. This requires the establishment of suitable protocols and can be labor and cost intensive. Therefore the 2015 iGEM Heidelberg team developed a aptamer design tool called MAWS (Making Aptamers Without SELEX) which tries to predict aptamers by progressive selection of appropriate bases using an entropic criterion. }}
 
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Revision as of 18:53, 31 October 2017

{{Heidelberg/main| 

   MAWS 2.0|
   Revised aptamer design software|
   URL to image|
   yellow|
   


    

    
 

         As aptamer-based riboswitches are the most promising regulatory element to couple the PACE/PREDCEL selection system to the intracellular level of a small molecule, the availbability of suitable aptamers is the limiting factor for enzyme PACE/PREDCEL experiments. The state of the art method to generate aptamers and riboswitches is based on the in vitro selection of sequences from a random oligonucleotide library using the SELEX method. This requires the establishment of suitable protocols and can be labor and cost intensive. Therefore the 2015 iGEM Heidelberg team developed a aptamer design tool called MAWS (Making Aptamers Without SELEX) which tries to predict aptamers by progressive selection of appropriate bases using an entropic criterion.

References