Difference between revisions of "Team:William and Mary/Parts"

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<!--UNS pdt#3a DT-->
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<a class="exp" href="#url" tabindex="1">UNS pdt#3a DT</a>
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<div class="exp">
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<div class="row">
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  <div class="col-sm-4" style='padding-left: 8 px; padding-top: 30px;'><IMG SRC="https://static.igem.org/mediawiki/2017/2/2f/T--William_and_Mary--part-1.jpeg" width=320px></div>
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  <div class="col-sm-8"style='padding-top: 0px; padding-right: 20px; font-size: 18px; font-family: "Optima";'>This part is designed to easily facilitate appending the protein degradation tag (pdt)#3 tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.<br> </br><br> iGEM registry: <a href= "http://parts.igem.org/Part:BBa_K2333401">BBa_K2333401</a> </br></div>
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<a class="exp" href="#url" tabindex="1">Part 1</a>
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<a class="exp" href="#url" tabindex="1">UNS pdt#3b DT</a>
 
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<div class="exp">
 
<p>
 
<p>
 
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   <div class="col-sm-3" style='padding-left: 8 px;'><IMG SRC="https://static.igem.org/mediawiki/2017/5/55/T--William_and_Mary--blank.jpeg" width=220px></div>
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   <div class="col-sm-4" style='padding-left: 8 px; padding-top: 30px;'><IMG SRC="https://static.igem.org/mediawiki/2017/2/2f/T--William_and_Mary--part-1.jpeg" width=320px></div>
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   <div class="col-sm-9"style='padding-top: 0px; padding-right: 20px; font-size: 22px; font-family: "Optima";'>Upcoming</div>
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   <div class="col-sm-8"style='padding-top: 0px; padding-right: 20px; font-size: 18px; font-family: "Optima";'>This part is designed to easily facilitate appending the protein degradation tag (pdt)#3 tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.<br> </br><br> iGEM registry: <a href= "http://parts.igem.org/Part:BBa_K2333401">BBa_K2333401</a> </br></div>
 
</div>
 
</div>
 
</p>
 
</p>
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</div>
  
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<!--UNS pdt#3c DT-->
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<a class="exp" href="#url" tabindex="1">UNS pdt#3c DT</a>
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<div class="exp">
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<p>
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<div class="row">
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  <div class="col-sm-4" style='padding-left: 8 px; padding-top: 30px;'><IMG SRC="https://static.igem.org/mediawiki/2017/2/2f/T--William_and_Mary--part-1.jpeg" width=320px></div>
 +
  <div class="col-sm-8"style='padding-top: 0px; padding-right: 20px; font-size: 18px; font-family: "Optima";'>This part is designed to easily facilitate appending the protein degradation tag (pdt)#3 tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.<br> </br><br> iGEM registry: <a href= "http://parts.igem.org/Part:BBa_K2333401">BBa_K2333401</a> </br></div>
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</div>
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</p>
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</div>
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<!--UNS pdt#3d DT-->
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<a class="exp" href="#url" tabindex="1">UNS pdt#3d DT</a>
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<div class="exp">
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<p>
 +
<div class="row">
 +
  <div class="col-sm-4" style='padding-left: 8 px; padding-top: 30px;'><IMG SRC="https://static.igem.org/mediawiki/2017/2/2f/T--William_and_Mary--part-1.jpeg" width=320px></div>
 +
  <div class="col-sm-8"style='padding-top: 0px; padding-right: 20px; font-size: 18px; font-family: "Optima";'>This part is designed to easily facilitate appending the protein degradation tag (pdt)#3 tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.<br> </br><br> iGEM registry: <a href= "http://parts.igem.org/Part:BBa_K2333401">BBa_K2333401</a> </br></div>
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</div>
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</p>
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</div>
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<!--UNS pdt#3e DT-->
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<a class="exp" href="#url" tabindex="1">UNS pdt#3e DT</a>
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<div class="exp">
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<p>
 +
<div class="row">
 +
  <div class="col-sm-4" style='padding-left: 8 px; padding-top: 30px;'><IMG SRC="https://static.igem.org/mediawiki/2017/2/2f/T--William_and_Mary--part-1.jpeg" width=320px></div>
 +
  <div class="col-sm-8"style='padding-top: 0px; padding-right: 20px; font-size: 18px; font-family: "Optima";'>This part is designed to easily facilitate appending the protein degradation tag (pdt)#3 tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.<br> </br><br> iGEM registry: <a href= "http://parts.igem.org/Part:BBa_K2333401">BBa_K2333401</a> </br></div>
 +
</div>
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</p>
 
</div>
 
</div>
  

Revision as of 03:32, 5 July 2017

UNS pdt#3 DT

This part is designed to easily facilitate appending the protein degradation tag (pdt)#3 tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.


iGEM registry: BBa_K2333401

UNS pdt#3a DT

This part is designed to easily facilitate appending the protein degradation tag (pdt)#3 tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.


iGEM registry: BBa_K2333401

UNS pdt#3b DT

This part is designed to easily facilitate appending the protein degradation tag (pdt)#3 tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.


iGEM registry: BBa_K2333401

UNS pdt#3c DT

This part is designed to easily facilitate appending the protein degradation tag (pdt)#3 tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.


iGEM registry: BBa_K2333401

UNS pdt#3d DT

This part is designed to easily facilitate appending the protein degradation tag (pdt)#3 tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.


iGEM registry: BBa_K2333401

UNS pdt#3e DT

This part is designed to easily facilitate appending the protein degradation tag (pdt)#3 tag to the end of an arbitrary protein using Gibson assembly, without requiring multiple cloning steps. UNS pdt#3 DT contains a tail that can be degrade Mesoplasma florum’s Lon protease, which is orthogonal to E. Coli’s own degradation machinery. As this part contains both a double stop codon and the B0015 double terminator, it can be added before the stop codons of an arbitrary protein, preventing a multistep assembly to incorporate double stop codons and a double terminator. Of this pdt tag series, this part has the highest degradation rate.


iGEM registry: BBa_K2333401