Difference between revisions of "Team:Heidelberg/Materials"
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Revision as of 21:11, 1 November 2017
Notebook
Materials
Antibiotics
| Name | Supplier | |
|---|---|---|
| Carbenicillin dissodium salt | Carl Roth | |
| Amicillin sodium salt | Carl Roth | |
| Chloramphenicol | Carl Roth | |
| Kanamycin sulfate | Sigma Aldrich | |
| Tetracycline | Sigma Aldrich | |
| Spectinomycine | Sigma Aldrich |
Enzymes
| Name | Supplier | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| BamI | New England BioLabs | |||||||||
| BamHI-HF | New England BioLabs | |||||||||
| BgIII | New England BioLabs | |||||||||
| BsaI-HF | New England BioLabs | |||||||||
| BsmBI | New England BioLabs | |||||||||
| DpnI | New England BioLabs | |||||||||
| EcoRI | New England BioLabs | |||||||||
| EcoRI-HF | New England BioLabs | |||||||||
| Esp3I | Thermo Fisher Scientific | |||||||||
| HindIII-HF | New England BioLabs | |||||||||
| Hinfl, recombinant | New England BioLabs | |||||||||
| HpaII | New England BioLabs | |||||||||
| KpnI-HF | New England BioLabs | |||||||||
| MfeI-HF | New England BioLabs | |||||||||
| MlyI, recombinant | New England BioLabs | |||||||||
| NheI-HF | New England BioLabs | |||||||||
| NotI-HF | New England BioLabs | |||||||||
| PacI | New England BioLabs | |||||||||
| PstI-HF | New England BioLabs | |||||||||
| PvuI-HF | New England BioLabs | |||||||||
| SalI-HF | New England BioLabs | |||||||||
| Sau3AI | New England BioLabs | |||||||||
| SpeI-HF | New England BioLabs | |||||||||
| XbaI | New England BioLabs |
Equipment
| Equipment | Name | Manufacturer | ||
|---|---|---|---|---|
| Table Centrifuge | Microfuge® 18 Centrifuge | Beckman CoulterTM | ||
| Table Centrifuge | Microfuge® 22R Centrifuge | Beckman CoulterTM | ||
| Centrifuge | Allegra X-12R | Beckman CoulterTM | ||
| Shaker | VORTEXGENIE 2 | Scientific Industries, SITM | ||
| Heating plate (magnet stirrer) | MR Hei-Standard | Heidolph | ||
| Heatblock | QBD2 | Grant | ||
| Heatblock | QBD4 | Grant | ||
| Heatblock (shaking function) | Thermomixer comfort | Eppendorf | ||
| PCR-Machine | MyCycler thermo cycler | BioRad | ||
| PCR-Machine | T100 Therma Cycler | BioRad | ||
| PCR-Machine | Mastercycler nexus X2 | Eppendorf | ||
| Gel documentation | Transluminator | Vilber Lourmat | ||
| Scale (fine) | PioneerTM PA114C | OHAUS | ||
| Scale | PioneerTM PA4101C | OHAUS | ||
| Incubator | KBW 400 | Binder | ||
| Incubator/ Shaker | ISF1-X | Kuhner shaker | ||
| Incubator / Shaker | HT Multitron Version 2 | INFORS | ||
| Plate Reader | Infinite M200 | Tecan | ||
| Plate Reader | GloMax® Discover | Promega | ||
| UV/VIS Spectrometer | Ultrospec 3300 pro | Amersham Biosciences | ||
| Microvulume spectrometer | NanoPhotometer NP80 | Implen | ||
| PH-Meter | PH-Meter 765 Calimatic | Knick | ||
| Lyophilizer | Gefriertrocknungsanlage ALPHA 1-2 LD Plus Best. Nr. 101521 | Christ | ||
| Sonificator | Sonoplus Gm 2070 (2002) | Bandelin | ||
| Vacuum Manifold | Qiavac 24 Plus | Qiagen | ||
| HPLC | HPLC system with binary pump, VWD detector and autosampler | Knauer | ||
| Nucleic acid purification system | QIAcube | Qiagen | ||
| PACE related equipment is specified here: |
Kits
| Name | Supplier | ||
|---|---|---|---|
| QIAquick® Gel Extraction Kit | Qiagen | ||
| QIAquick® PCR Purification Kit | Qiagen | ||
| QIAprep® Spin Miniprep Kit | Qiagen | ||
| DNA Clean & Concentrator™-5 | Zymo Research | ||
| ZymoPURE™ Plasmid Miniprep Kit | Zymo Research | ||
| ZymoPURE™ Plasmid Midiprep Kit | Zymo Research | ||
| NEB iGEM Support Kit 2 | New England Biolabs | ||
| NEB Quick Cloning Box-HF | New England Biolabs | ||
| HiScribe T7 Quick High Yield RNA Synthesis Kit | New England Biolabs | ||
| MinION Basic starter pack (1 MinION, 2 Flow cells, 1 Sequencing Kit, 1 Wash kit) | Oxford Nanopore |
Markers
| Name | Supplier | ||
|---|---|---|---|
| Quick-Load 2-Log DNA Ladder (0.1-10.0 kb) | New England BioLabs | ||
| Quick-Load 1 kb DNA Ladder | New England BioLabs | ||
| 100 bp DNA Ladder | New England BioLabs | ||
| Novex® Sharp Pre-stained Protein Standard | Invitrogen | ||
| SERVA Triple Color Protein Standard | SERVA Electrophoresis |
Media
| Media | Composition | |
|---|---|---|
| LB medium (Luria/Miller für d-2,5 kg Best. Nr.: X968.3 | 10 g NaCl, 10 g tryptone/ peptone, 5 g yeast extract in 1 L dH2O | |
| 2xYT medium für die Molekularb-2,5 kg Best. Nr.: X966.3 | 5 g NaCl, 16 g tryptone/ peptone, 10 g yeast extract in 1 L dH2O | |
| SOB medium | 0,5 % yeast extract, 2 % tryptone/peptone, 10 mM NaCl, 2.5 mM KCl, 10 mM MgCl2, 10 mM MgSO4 | |
| SOB medium | SOB supplemented with 20 mM glucose | |
| M9 minimal medium |
Other Enzymes
| Name | Supplier | ||||||
|---|---|---|---|---|---|---|---|
| Gibson Assembly® Master Mix | New England Biolabs | ||||||
| T4 DNA Ligase | New England Biolabs | ||||||
| T7 RNA Polymerase | New England Biolabs | ||||||
| T4 Polynucleotide Kinase (PNK) | New England Biolabs | ||||||
| T4 RNA Ligase 1 (ssRNA Ligase) | New England Biolabs | ||||||
| Alkaline Phosphatase (CIP) | New England Biolabs | ||||||
| Shrimp Alkaline Phosphatase (rSAP) | New England Biolabs |
Polymerases
| Name | Supplier | ||||||
|---|---|---|---|---|---|---|---|
| OneTaq® Quick-Load® 2X Master Mix with Standard Buffer | New England Biolabs | ||||||
| Phusion Flash High-Fidelity PCR Master Mix | Thermo Fisher Scientific | ||||||
| Q5® High-Fidelity DNA Polymerase 2X Master Mix | New England Biolabs |
